-
Expression and catalytic activity of the tyrosine phosphatase PTP1C is severely impaired in motheaten and viable motheaten miceKozlowski, M... ...Mutations in the gene encoding the phosphotyrosine phosphatase PTP1C, a cytoplasmic protein containing a COOH-terminal catalytic and two NH2-terminal Src homology 2 (SH2) domains, have been ... identified in motheaten (me) and viable motheaten (mev) mice and are associated with severe hemopoietic dysregulation. The me mutation is predicted to result in termination of the PTP1C polypeptide within the first SH2 domain, whereas the mev mutation creates an insertion or deletion in the phosphatase domain. No PTP1C RNA or protein could be detected in the hemopoietic tissues of me mice, nor could PTP1C phosphotyrosine phosphatase activity be isolated from cells homozygous for the me mutation. In contrast, mice homozygous for the less severe mev mutation expressed levels of full-length PTP1C protein comparable to those detected in wild type mice and the SH2 domains of mev PTP1C bound normally to phosphotyrosine-containing ligands in vitro. Nevertheless, the mev mutation induced a marked reduction in PTP1C activity. These observations provide strong evidence that the motheaten phenotypic results from loss-of-function mutations in the PTP1C gene and imply a critical role for PTP1C in the regulation of hemopoietic differentiation and immune function.Source: The Journal of experimental medicine. - ISSN 0022-1007 (Letn. 178, št. 6, 1993, str. 2157-2163)Type of material - article, component partPublish date - 1993Language - englishCOBISS.SI-ID - 2612441
Author
Kozlowski, M... |
Mlinarič-Raščan, Irena |
Feng, G... S. |
Shen, R... |
Pawson, T... |
Siminovitch, K... A.
Topics
Hematopoiesis |
Mice, mutant strains |
Genetics |
Protein-tyrosine-phosphatase |
Metabolism |
Base sequence |
Dna primers |
Chemistry |
Gene expression |
Mice |
Mice, inbred c3h |
Mice, inbred c57bl |
Molecular sequence data |
Pedigree |
Phosphoproteins |
Metabolism |
Point mutation |
Protein-tyrosine-phosphatase |
Genetics |
Receptors, epidermal growth factor-urogastrone |
Metabolism |
Receptors, platelet-derived growth factor |
Metabolism |
Rna, messenger |
Genetics |
Signal transduction
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|---|
Mlinarič-Raščan, Irena | 12443 |
Select pickup location:
Material pickup by post
Notification
Subject headings in COBISS General List of Subject Headings
Select pickup location
Pickup location | Material status | Reservation |
---|
Please wait a moment.