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Intermediate filaments attenuate stimulation-dependent mobility of endosomes/lysosomes in astrocytesPotokar, Maja, 1973- ...Intermediate filament (IF) proteins upregulation is a hallmark of astrocyte activation and reactive gliosis, but its pathophysiological implications remain incompletely understood. A recently ... reported association between IFs and directional mobility of peptidergic vesicles allows us to hypothesize thatIFs affect vesicle dynamics and exocytosis-mediated astrocyte communication with neighboring cells. Here, we ask whether the trafficking of recycling vesicles (i.e., those fused to and then retrieved from the plasma membrane) and endosomes/lysosomes depends on IFs. Recycling vesicles were labeled by antibodies against vesicle glutamate transporter 1 (VGLUT1) and atrial natriuretic peptide (ANP), respectively, and by lysotracker, which labels endosomes/lysosomes. Quantitative fluorescence microscopy was used to monitor the mobility of labeled vesicles in astrocytes, derived from either wild-type (WT) mice or mice deficient in glial fibrillary acidic protein and vimentin (GFAP-/-Vim-/-), the latter lacking astrocyte IFs. Stimulation with ionomycin or ATP enhanced the mobility of VGLUT1-positive vesicles and reducedthe mobility of ANP-positive vesicles in WT astrocytes. In GFAP-/-Vim-/- astrocytes, both vesicle types responded to stimulation, but the relative increase in mobility of VGLUT1-positive vesicles was more prominent compared with nonstimulated cells, whereas the stimulation-dependentattenuation of ANP-positive vesicles mobility was reducedcompared with nonstimulated cells. The mobility of endosomes/lysosomes decreased following stimulation in WT astrocytes. However, in GFAP-/-Vim-/- astrocytes, a small increase in the mobility of endosomes/lysosomes was observed. These findings show that astrocyte IFs differentially affect the stimulation-dependent mobility of vesicles. We propose that upregulation of IFs in pathologic states may alter the function of astrocytes by deregulating vesicle trafficking.Source: Glia. - ISSN 0894-1491 (Letn. 58, št. 10, 2010, str. 1208-1219)Type of material - article, component partPublish date - 2010Language - englishCOBISS.SI-ID - 27046873
Author
Potokar, Maja, 1973- |
Stenovec, Matjaž |
Gabrijel, Mateja |
Li, Lizhen |
Kreft, Marko |
Grilc, Sonja |
Penky, Miloš |
Zorec, Robert, 1958-
Topics
Astrocytes |
Drug Effects |
Endosomes |
Lysosomes |
Intermediate Filament Proteins |
Immunohistochemistry |
Atrial Natriuretic Factor |
Glial Fibrillary Acidic Protein |
Vimentin |
Microscopy, Confocal |
Ionomycin |
Cells, Cultured |
Mice, Inbred Strains |
Lizosomi |
Imunohistokemija |
Astrociti |
Endosomi |
Srednje vlakno beljakovine |
Glialna, fibrilarna, kisla beljakovina |
Vimentin |
Mikroskopija konfokalna |
Ionomicin |
Celične kulture |
Miši, čiste linije |
Preddvorni, natriuretični faktor
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Potokar, Maja, 1973- | 21390 |
Stenovec, Matjaž | 15467 |
Gabrijel, Mateja | 23345 |
Li, Lizhen | |
Kreft, Marko | 15666 |
Grilc, Sonja | 06846 |
Penky, Miloš | |
Zorec, Robert, 1958- | 03702 |
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