-
Cholesterol and regulated exocytosis : a requirement for unitary exocytotic eventsRituper, Boštjan ...Since the 1970s, much effort was been expended researching mechanisms of regulated exocytosis. Early work focused mainly on the role of proteins. Most notably the discovery of SNARE proteins in the ... 1980s and the zippering hypothesis brought us much closer to understanding the complex interactions inmembrane fusion between vesicle and plasma membranes, a pivotal component ofregulated exocytosis. However, most likely due to the predictions of the Singer-Nicholson fluid mosaic membrane model, the lipid components of the exocytotic machinery remained largely overlooked. Lipids were considered passive constituents of cellular membranes, not contributing much, if anything, to the process of exocytosis and membrane fusion. Since the 1990s, this so-called proteocentric view has been gradually giving way to the new perspective best described with the term proteolipidic. Many lipids were foundto be of great importance in the regulation of exocytosis. Here we highlight the role of cholesterol. Furthermore, by using high-resolution cell-attached membrane capacitance measurements, we have monitored unitary exocytotic events in cholesterol-depleted membranes. We show that the frequency of these events is attenuated, providing evidence at the single vesicle level that cholesterol directly influences the merger of the vesicle and the plasma membranes.Source: Cell calcium. - ISSN 0143-4160 (Vol. 52, iss. 3/4, 2012, str. 250-258)Type of material - article, component partPublish date - 2012Language - englishCOBISS.SI-ID - 30118361
Author
Rituper, Boštjan |
Flašker, Ajda |
Guček, Alenka, 1987- |
Chowdhury Haque, Helena |
Zorec, Robert, 1958-
Topics
Exocytosis |
Cholesterol |
Metabolism |
Membrane Fusion |
Cyclodextrins |
Membrane Lipids |
Calcium |
Electric Conductivity |
Eukaryotic Cells |
Eksocitoza |
Holesterol |
Membrana, zlitje |
Ciklodekstrini |
Membranski lipidi |
Električna prevodnost |
Kalcij |
Evkariontske celice
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|---|
Rituper, Boštjan | 32000 |
Flašker, Ajda | 32068 |
Guček, Alenka, 1987- | 34319 |
Chowdhury Haque, Helena | 18548 |
Zorec, Robert, 1958- | 03702 |
Select pickup location:
Material pickup by post
Notification
Subject headings in COBISS General List of Subject Headings
Select pickup location
Pickup location | Material status | Reservation |
---|
Please wait a moment.