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6-arylpyrido[2,3-d]pyrimidines as novel ATP-competitive inhibitors of bacterial D-alanine: D-alanine ligaseŠkedelj, Veronika, 1983- ...Background: ATP-dependent D-alanine:D-alanine ligase (Ddl) is a part of biochemical machinery involved in peptidoglycan biosynthesis, as it catalyzes the formation of the terminal D-ala-D-ala ... dipeptide of the peptidoglycan precursor UDPMurNAc pentapeptide. Inhibition of Ddl prevents bacterial growth, which makes this enzyme an attractive and viable target in the urgent search of novel effective antimicrobial drugs. To address the problem of a relentless increase in resistance to known antimicrobial agents we focused our attention to discovery of novel ATP-competitive inhibitors of Ddl. Methodology/Principal Findings: Encouraged by recent successful attempts to find selective ATP-competitive inhibitors of bacterial enzymes we designed, synthesized and evaluated a library of 6-arylpyrido[2,3-d]pyrimidine-based compounds as inhibitors of Escherichia coli DdlB. Inhibitor binding to the target enzyme was subsequently confirmed by surface plasmon resonance and studied with isothermal titration calorimetry. Since kinetic analysis indicated that 6-arylpyrido[2,3- d]pyrimidines compete with the enzyme substrate ATP, inhibitor binding to the ATP-binding site was additionally studied with docking. Some of these inhibitors were found to possess antibacterial activity against membrane-compromised and efflux pump-deficient strains of E. coli. Conclusions/Significance: We discovered new ATP-competitive inhibitors of DdlB, which may serve as a starting point for development of more potent inhibitors of DdlB that could include both, an ATP-competitive and D-Ala competitive moiety.Source: PloS one [Elektronski vir]. - ISSN 1932-6203 (Vol. 7, no. 8, 2012, str. e39922-1-e39922-14)Type of material - e-articlePublish date - 2012Language - englishCOBISS.SI-ID - 3286897
Author
Škedelj, Veronika, 1983- |
Arsovska, Emilija |
Tomašič, Tihomir |
Kroflič, Ana, KI |
Hodnik, Vesna, 1979- |
Hrast, Martina |
Bešter-Rogač, Marija |
Anderluh, Gregor, 1969- |
Gobec, Stanislav, 1970- |
Bostock, Julieanne M. |
Chopra, Ian |
O'Neill, Alex |
Randall, Christopher |
Zega, Anamarija
Topics
D-alanin ligaze |
bakterijska odpornost |
Ddl inhibitorji |
antibiotiki |
sinteza
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Škedelj, Veronika, 1983- | 29903 |
Arsovska, Emilija | 92267 |
Tomašič, Tihomir | 28334 |
Kroflič, Ana, KI | 31996 |
Hodnik, Vesna, 1979- | 29235 |
Hrast, Martina | 32036 |
Bešter-Rogač, Marija | 04657 |
Anderluh, Gregor, 1969- | 15686 |
Gobec, Stanislav, 1970- | 15284 |
Bostock, Julieanne M. | |
Chopra, Ian | |
O'Neill, Alex | |
Randall, Christopher | |
Zega, Anamarija | 18633 |
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