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The ESR kinetic study of lipid phase in HDLBrnjas-Kraljević, Jasminka ...Two main high-density lipoprotein subfractions, HDL2 and HDL3, were spin labelled with MPO which partitions both in aqueous and lipid phase. The dynamics of the lipid phase was monitored via the ... reduction of incorporated TEMPO with ascorbic acid. The reduction of the paramagnetic nitroxide into the nonparamagnetic hydroxylamine form decreases the ESR signal with time. The reduction curves show complex behaviour while the partition coeffcients of TEMPO remain unchanged during thereaction. The reduction process in the samples containing HDL particles proceeds faster than in the aqueous solution of pure reactants, e.g. spin label and ascorrbic acid. In order to explain experimental data the model for reduction of TEMPO by ascorbic acid is proposed. It assumes that the processes taking place in these heterogeneous systems are determined by the overall reaction rate, which depends on the local concentrations of the reactants, as well as on their transport properties in the particular phases.Source: Croatica chemica acta. - ISSN 0011-1643 (Vol. 74, no. 1, 2001, str. 147-160)Type of material - article, component partPublish date - 2001Language - englishCOBISS.SI-ID - 888689
Author
Brnjas-Kraljević, Jasminka |
Kveder, Marina |
Pifat, Greta |
Pečar, Slavko |
Schara, Milan Valter
Topics
ESR |
lipoproteini |
HDL |
kinetika |
redukcija
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Database name | Field | Year |
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Pečar, Slavko | 03070 |
Schara, Milan Valter | 00393 |
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