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In vitro mutagenesis of the GGS1/TPS1 gene of Sacharomyces cerevisiae : graduation thesis = In vitro mutacija gena GGS1/TPS1 kvasovke Saccharomyces cerevisiae : diplomska nalogaBuzeti, TatjanaIn vitro mutacije na genu GGS1/TPS1 kvasovke Saccharomyces cerevisiae so potekale z naključno in s ciljano mutagenezo. Rezultat ciljane mutageneze so bile 3 mutacije, D395A, H185A in N295A. S ... plazmidi Ycplac33/TPS1, ki so vsebovali mutagene alele, sem transformirala seve W303-1Atps1[delta] in W303-1Atps1[delta]tps2[delta]. Na podlagi opazovanja fenotipskih sprememb mutantov na selektivnem gojišču sem opazovala spremembo v glikolitičnem fluksu oz. njihovo rast na glukozi ter termotoleranco. Aktivnost trehaloza-6-fosfat sintaze (aktivnost Tps1) in vsebnost trehaloze sem spremljala z biokemičnimi metodami. Pri vseh treh mutacijah je bila popolnoma uničena aktivnost Tps1, posledica česar je tudi minimalna vsebnost trehaloze v primeru mutacij D395A in H185A. V primeru mutacije N295A je bila vsebnost trehaloze nekoliko višja, s čimer bi lahko razlagali še vedno obstoječo regulacijo glukoznega fluksa v glikolizo in s tem rast na glukoznem gojišču. Rezultati potrjujejo vlogo gena GGS1/TPS1 v glikolitičnem fluksu, vendar ne moremo potrditi niti zavreči nobene izmed treh predlaganih razlag njegove regulatorne vloge v omenjeni metabolni poti.Type of material - undergraduate thesis ; adult, seriousPublication and manufacture - Ljubljana : [BF, Oddelek za živilstvo] : [T. Buzeti], 1999Language - englishCOBISS.SI-ID - 2262904
Author
Buzeti, Tatjana
Other authors
Raspor, Peter |
Smole Možina, Sonja
Topics
Saccharomyces cerevisiae |
mutacije |
Gen GGS1/TPS1 |
trehaloza-6-fosfat sintaza |
trehaloza |
glikoliza |
termotoleranca mutantov |
termotoleranca mutantov |
Saccharomyces cerevisiae |
mutations |
GGS1/TPS1 gene |
trehalose-6-phosphate synthase |
trehalose |
glycolysis |
thermotolerance of the mutants |
thermotolerance of the mutants
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BF, Dept. of Food Science and Technology, Lj. |
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Call number – location, accession no. ... |
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Hodnik DN 0000000915 IN: 0011570 Hodnik DN 915 IN: 0011570 |
available - outside loan, loan period: 1 months
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Hodnik DN 0000000915 a IN: 0011571 Hodnik DN 915 a IN: 0011571 |
available - outside loan, loan period: 1 months
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Raspor, Peter | 04001 |
Smole Možina, Sonja | 07030 |
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