BF, Dept. of Food Science and Technology, Lj. (BFZIV)
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Antigenska in molekularna določitev humanih rotavirusov : diplomska naloga = Antigenic and molecular determination of human rotaviruses : graduation thesisKoblič, AndrejaRotavirusi so pomembnejǴi virusni enteropatogeni in predstavljajo glavni vzrokza akutno drisko pri otrocih. Vzorce iztrebkov smo dobili od otrok z akutnim gastroenteritisom, pri katerih smo z ... encimsko imunskim testom ali elektronsko mikroskopijo ugotovili rotavirusno okuÍbo. Z encimsko imunskim testom smo dolořili skupinsko specifiřen antigen (VP6) za rotaviruse skupine A. Z veriÍno reakcijo s polimerazo smo iz vzorcev iztrebka pomnoÍili odsek gena, ki kodira osnovni zunanji kapsidni glikoprotein VP7. Iz vzorcev iztrebkov smo izolirali dvovijařno RNA in jo uporabili kot matrico v reverzni transkripciji s pomnoÍevanjem, kjer smo uporabili zařetni oligonukleotid, s katerim smo pomnoÍili celoten odsek. Z uporabo Ǵestih tipsko specifiřnih zařetnih oligonukleotidov, ki pomnoÍijo le dolořeno podrořje celotnega gena 9,smo dolořili serotipe G. V obdobju jesen/zima 1999/2000 smo pri vseh vzorcih(46) dolořili serotip G1, pri vzorcih iz leta 1993 serotipa G1 in G4 (9 vzorcev spada v serotip G1, 5 vzorcev spada v serotip G4 in 6 vzorcev predstavlja serotip G1 in G4).Type of material - undergraduate thesis ; adult, seriousPublication and manufacture - Ljubljana : [A. Koblič] ; [BF, Enota medoddelčnega študija mikrobiologije], 2000Language - slovenianCOBISS.SI-ID - 2387320
Author
Koblič, Andreja
Other authors
Drinovec, Borut |
Poljšak-Prijatelj, Mateja
Topics
gastroenteritis |
virusi |
humani rotavirusi |
serotipi G (VP7) |
RT-PCR |
RT-PCR |
gastroenteritis |
viruses |
human rotaviruses |
serotypes G (VP7) |
RT-PCR |
RT-PCR
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Študij mikrobiologije DN 0000000049 a IN: 0012056 Študij mikrobiologije DN 49 a IN: 0012056 |
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Študij mikrobiologije DN 0000000049 IN: 0012055 Študij mikrobiologije DN 49 IN: 0012055 |
available - outside loan, loan period: 1 months
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Drinovec, Borut | 03174 |
Poljšak-Prijatelj, Mateja | 08755 |
Source: Personal bibliographies
and: SICRIS
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