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BF, Dept. of Animal Science, Ljubljana (BFZOO)
  • Characterization of the mouse lanosterol 14[alfa]-demetylase (CYP51), a new member of the evolutionarily most conserved cytochrome P450 family
    Debeljak, Nataša ...
    Genes encoding sterol 14[alfa]-demethylases in eukaryotes and in Mycobacterium to the CYP51 family which is evolutionary the most conserved gene family within the cytochrome P450 superfamily. We have ... characterized a new member of this family, the mouse lanosterol 14]alfa]-demethylase, with the aim to study the in vivo role of this gene in spermatogenesis in mammals. The amino acid sequence of mouse Cyp51 is 96% to human. Comparion of all known CYP51 proteins by the neighbor-joining method suggests that fungal and animal CYP51 genes arose from a common ancestral gene (98.3% probability) and interestingly, that plant and bacterial CYP51 genes share a common progenitor (88.8% probability). This suggests that the first CYP51 gene may have arisen in eukaryotes and has been transferred horizontally from plants to Mycobacterium. The mouse CYP51 gene is ~17-kb long and contains 10 exons. Transcription starts at several locations within the CpG island, which is characteristic for the TATA-less housekeeping genes. The mouse 5ʹ-untranslatedregion (800 bp) contains putative cAMP-responsive elements (CRE), sterol regulatory elements (SRE) and GC-boxes at positions similar to human and rat, sugesting an evolutionary conserved mechanism of CYP51 transcriptional regulation in mammals. The mouse Cyp51 gene resides on chromosome 5, region A2, close to the centromere. No signals, outside this region were detected as well as no evidence of processed pseudogenes using long PCR was found. This indicates that the mouse genome most likely lacksCYP51 processed pseudogenes.
    Source: Archives of biochemistry and biophysics. - ISSN 0003-9861 (Vol. 379, no. 1, 2000, str. 37-45)
    Type of material - article, component part
    Publish date - 2000
    Language - english
    COBISS.SI-ID - 1000072

source: Archives of biochemistry and biophysics. - ISSN 0003-9861 (Vol. 379, no. 1, 2000, str. 37-45)

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