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Faculty of Pharmacy, Lj. (FFALJ)
  • Simultaneous determination of gabapentin, pregabalin, vigabatrin, and topiramate in plasma by HPLC with fluorescence detection
    Martinc, Boštjan ...
    Therapeutic drug monitoring (TDM) of antiepileptic drugs (AEDs) has been recognized as a useful tool in management of epilepsy. We developed a simple analytical method for simultaneous determination ... of four second generation AEDs, including gabapentin (GBP), pregabalin (PGB), vigabatrin (VGB), and topiramate (TOP). Analytes were extracted from human plasma using universal solid phase extraction, derivatized with 4-chloro-7-nitrobenzofurazan (NBD-Cl)and analysed by HPLC with fluorescence detection. Using mass spectrometry we confirmed that NBD-Cl reacts with sulfamate group of TOP similarly as with amine group of the other three analytes. The method is linear (r2 > 0.998) across investigated analytical ranges (0.375 - 30.0 micro g/mL for GBP, PGB, and VGB; 0.50 - 20.0 micg/mL for TOP). Intraday and interday precision do not exceed 9.40%. The accuracy is from 95.6% to 106%. The recovery is higher than 80.6%, and the lower limit of quantification is at least 0.5 microg/mL. The method is selective and robust. For TOP determination method was compared to a previously published method and the results obtained by the two methods were in good agreement. The developed method is suitable for routine TDM.
    Source: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. - ISSN 1570-0232 (Vol. 962, Jul. 2014, str. 82-88)
    Type of material - article, component part
    Publish date - 2014
    Language - english
    COBISS.SI-ID - 3657841

    Link(s):

    http://www.sciencedirect.com/science/article/pii/S1570023214003389#
    DOI

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