Maize pests like Ostrinia nubilalis and Diabrotica virgifera virgifera are eradicated using genetically modified maize. This study’s goal was to see if the genetically modified maize MON810 is also ...toxic to thrips communities on maize. The impact of Bt maize on thrips diversity and abundance, as well as yield losses, was studied in the field in Borovce for three years (Slovakia). The study used 10 Bt and 10 non-Bt maize cultivars. Thrips were monitored every two weeks during the season using transparent sticky traps installed on the experimental plots (one per plot, 20 per year). In total, 3426 thrips were caught. Thrips populations usually peak around the end of July at BBCH55. Among the species identified were Limothrips denticornis, Limothrips cerealium, Haplothrips aculeatus, Frankliniella schultzei, Frankliniella occidentalis, Thrips tabaci, Aeolothrips fasciatus, Frankliniella tenuicornis, and Chirothrips spp. We found that MON810 maize had no effect on the occurrence or composition of thrips. Their presence was affected by the maize growth phase and growing seasons and partially by the weather. The direct effect on the grain yield was not confirmed. Our research contributed to scientific knowledge of thrips communities found on maize plants in Central Europe, including Bt maize.
Scaphoideus titanus Ball, the main vector of Flavescence dorée phytoplasma, was monitored in Slovakia during 2013 and 2014. The species was present in the vineyards of eastern Slovakia but it was ...rarely found in the south of central part of the country. It was also found at many locations of western Slovakia but not in the southeast of western Slovakia. Higher abundance of S. titanus was detected in the vineyards with biological control. Varietal preference of the leafhopper was not confirmed. The first nymphs were found on June 5 and the last nymphs on July 17, adults occurred from the end of June to the end of September. There is one generation of S. titanus per year in Slovakia.
•Male Wistar were exposed to repeated ip injection with nicotine for 7 days.•On the 8th and the 9th day rats were injected icv with antalarmin or astressin2B.•Horizontal and vertical locomotor ...activities changed on the 8th and the 9th day.•Dorsal and ventral striatal dopamine releases changed on the 8th and the 9th day.•All the changes observed were attenuated by antalarmin, but not astressin2B.
The aim of the present study was to investigate the participation of corticotropin-releasing factor (CRF) receptors (CRF1 and CRF2) in the alterations of the dorsal and ventral striatal dopamine release and the vertical and horizontal locomotor activity observed in rats following chronic nicotine treatment and consequent acute withdrawal. In this purpose, male Wistar rats were exposed to repeated intraperitoneal (ip) injection with nicotine or saline solution for 7 days. On the 8th day or the 9th day the rats were injected intracerebroventricularly (icv) with selective CRF1 antagonist antalarmin or selective CRF2 antagonist astressin2B or saline solution. Thirty minutes after the icv injection the changes of the horizontal and vertical locomotor activity were recorded in an in vivo conducta system. Immediately after the behavioral recordings the changes of the dorsal and ventral striatal dopamine release were determined in an in vitro superfusion system. On the 8th day, the horizontal and vertical locomotor activities and the dorsal and ventral striatal dopamine releases increased significantly in nicotine-treated rats, compared to the saline-treated ones. On the 9th day, the horizontal locomotor activity and the dorsal striatal dopamine release increased significantly, whereas the vertical locomotor activity and the ventral striatal dopamine release decreased significantly in nicotine-treated rats, compared to the saline-treated ones. All the changes observed were attenuated significantly by antalarmin, but not astressin2B. The present study demonstrates that the changes of striatal dopamine release and locomotor activity observed following chronic nicotine treatment and consequent acute withdrawal are mediated by CRF1, but not CRF2, receptor.
Abstract The aim of the present study was to investigate the effects of the selective agonists of the corticotropin-releasing factor (CRF) 2 receptor, urocortin 2 (UCN 2) and urocortin 3 (UCN 3), on ...the anxiety- and depression-like signs induced by acute nicotine withdrawal in mice. In order to do so, male CFLP mice were exposed for 7 days to repeated intraperitoneal (IP) injection with nicotine or saline solution and 1 day of acute withdrawal and then a single intracerebroventricular (ICV) injection with UCN 2, UCN 3 or saline solution. After 30 minutes the mice were observed in an elevated plus-maze test or a forced swim test, for anxiety- and depression-like behavior. After 5 minutes of testing, the plasma corticosterone concentration reflecting the activity of the hypothalamic-pituitary-adrenal (HPA) axis was also determined by a chemo-fluorescent method. Half of the animals were treated ICV and evaluated on the 8th day, the other half on the 9th day. On the 8th day, nicotine-treated mice presented signs of anxiolysis and depression, but no significant elevation of the plasma corticosterone concentration. On the 9th day, nicotine-treated mice exhibited signs of anxiety and depression and a significant increase of the plasma corticosterone levels. Central administration of UCN 2 or UCN 3 ameliorated the anxiety- and depression-like state including the hyperactivity of the HPA axis, developed during acute withdrawal following chronic nicotine treatment. The present study suggests that selective CRF2 receptor agonists could be used as a therapy in nicotine addiction.
The aim of the present study was to determine the effects of alcohol intoxication and withdrawal on hypothalamic neurohormones such as corticotropin-releasing factor (CRF) and arginine vasopressin ...(AVP), and extrahypothalamic neurotransmitters such as striatal dopamine (DA), amygdalar gamma aminobutyric acid (GABA), and hippocampal glutamate (GLU). In addition, the participation of the two CRF receptors, CRF1 and CRF2, was investigated. For this purpose, male Wistar rats were exposed to repeated intraperitoneal (ip) administration of alcohol every 12 h, for 4 days and then for 1 day of alcohol abstinence. On the fifth or sixth day, intracerebroventricular (icv) administration of selective CRF1 antagonist antalarmin or selective CRF2 antagonist astressin
B was performed. After 30 min, the expression and concentration of hypothalamic CRF and AVP, the concentration of plasma adrenocorticotropic hormone (ACTH) and corticosterone (CORT), and the release of striatal DA, amygdalar GABA, and hippocampal GLU were measured. Our results indicate that the neuroendocrine changes induced by alcohol intoxication and withdrawal are mediated by CRF1, not CRF2, except for the changes in hypothalamic AVP, which are not mediated by CRF receptors.
Byzantine-Fault-Tolerant (BFT) state machine replication is an appealing technique to tolerate arbitrary failures. However, Byzantine agreement incurs a fundamental trade-off between being fast (i.e. ...optimal latency) and achieving optimal resilience (i.e. 2f + b + 1 replicas, where f is the bound on failures and b the bound on Byzantine failures). Achieving fast Byzantine replication despite f failures requires at least f + b - 2 additional replicas. In this paper we show, perhaps surprisingly, that fast Byzantine agreement despite f failures is practically attainable using only b - 1 additional replicas, which is independent of the number of crashes tolerated. This makes our approach particularly appealing for systems that must tolerate many crashes (large f) and few Byzantine faults (small b). The core principle of our approach is to have replicas agree on a quorum of responsive replicas before agreeing on requests. This is key to circumventing the resilience lower bound of fast Byzantine agreement.
Rows of cells that flank the parasegment boundary make up a signaling center within the epidermis of the Drosophila embryo. Signals emanating from these cells, encoded by hedgehog (hh) and wingless ...(wg), are shown to be required for all segment pattern dorsally. Wg activity is required for the differentiation of one cell type, constituting half the parasegment. The gene lines appears to act in parallel to the Wg pathway in the elaboration of this cell type. Hh activity is responsible for three other cell types in the parasegment. Some cell types are specified as Hh activity and interfere with the function of patched, analogous to patterning of imaginal discs. However, some pattern is independent of the antagonism of patched by Hh, and relies instead on novel interactions with lines. Lastly, we provide evidence that decapentaplegic does not mediate patterning by Hh in the dorsal epidermis.
Abstract
A large landfill-gas-to-power facility retained the authors to provide process evaluation and development support for a conceptual design of a landfill gas condensate (LFGC) treatment system ...to eliminate odor issues and reduce organic loads to the sewer or use the water for dust control. Based on the operational observations and water quality data, the source of the LFGC is moisture from gas generated by the decay of waste in the landfill that is transmitted to the power generation facilities by way of the landfill gas (LFG) collection system. As part of this project, the authors reviewed the data, and this presentation includes recommendations for long-term treatment solutions so that a permanent system can be designed, bid, procured, and installed. The purpose of the abstract is to present the findings of this analysis of LFGC treatment system alternatives to identify practical, commercially available solutions with the primary goal to reduce odor issues related to LFGC both on-site and in the sewer system, and to reduce water usage at the site for dust control. The method to evaluate the various treatment options was based on treatment efficiency, and capital and life-cycle costs. The conclusion was to continue with pilot testing of the most suitable options, which was anaerobic treatment followed by polishing with a moving bed bioreactor (MBBR) to reuse the product water in cooling towers or for dust control.
The fungus Macrophomina phaseolina distributed especially in warmer region of Europe is reported for the first time from the Slovakia as a pathogen of sunflower. Morphology of its populations is ...characterized.
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