Both the ISO 6579:2002 method and the BacTrac 4300 - Impedance system were applied for the detection of Salmonella enterica from pigs at slaughter. A total of 68 pigs, reared in 62 different farms of ...Northern Italy, were randomly selected during 9 consecutive visits at two slaughter-houses. A total of 204 samples (68 tonsils, 68 samples of caecal matter and 68 ham area carcass swabs) were collected. S. enterica was isolated from 8 (11.8%) tonsils, 17 faecal samples (25.0%) and from 10 (14.7%) carcass swabs. The ISO method detected as positive 6 (75.0%) tonsils, 14 (82.4%) faecal samples and 9 (90.0%) carcasses. S. enterica was isolated from 8 (100%) tonsils, 14 (82.4%) faecal samples and 10 (100%) carcass swabs by the BacTrac 4300 - Impedance system. Salmonella strains serotyping and phagetyping identified 15 S. Derby, 5 S. Agona, 4 S. Rissen, 2 S. Typhimurium phage-type U302, 2 S. Typhimurium DT120, 2 S. enterica 1, 4, 5, 12:i.-, 2 S. Kapemba, 1 S. Give, 1 S. Anatum and 1 S. enterica non-typeable (R strain).
We have measured the radiative decays
φ→
ηγ,
φ→
η′
γ selecting
π
+
π
−
γγγ final state in a sample of ∼5×10
7
φ-mesons produced at the Frascati
φ-factory DA
ΦNE. We obtain
Γ(
φ→
η′
γ)/
Γ(
φ→
...ηγ)=(4.70±0.47±0.31)×10
−3. From this result we derive new accurate values for the branching ratio BR(
φ→
η′
γ)=(6.10±0.61±0.43)×10
−5 and the mixing angle of pseudoscalar mesons in the flavour basis
ϕ
P
=(41.8
+1.9
−1.6)°.
Following favourable results from a previous study, a large, multicentre, prospective, case-control study was performed to further assess the incidence of bleeding complications after dental ...extraction in patients taking oral anticoagulant therapy (OAT). Four hundred fifty-one patients being treated with warfarin who required dental extraction were compared with a control group of 449 non-anticoagulated subjects undergoing the same procedure. In the warfarin-treated group, the oral anticoagulant regimen was maintained unchanged, such that the patients had an International Normalised Ratio ranging between 1.8 and 4, and local haemostatic measures (i.e. fibrin sponges, silk sutures and gauzes saturated with tranexamic acid) were adopted. All the procedures were performed in an outpatient setting. Seven bleeding complications occurred in the OAT group and four in the control group; the difference in the number of bleeding events between the two groups was not statistically significant (OR=1.754; 95% CI 0.510 – 6.034; p=0.3727). No post-operative late bleeds requiring hospitalisation and/or blood transfusions were recorded, and the adjunctive local haemostatic measures were adequate to stop the bleeding. The results of our protocol applied in this large, multicenter study show that dental extractions can be performed easily and safely in anticoagulated outpatients without any modification of the ongoing anticoagulant therapy, thus minimising costs and reducing discomfort for patients.
The official biotoxicological (MBA) method for the determination of lipophilic marine toxins shows important gaps with regard to reproducibility, specificity and quality of the data provided and ...serious ethical contraindications. The Commission Regulation (EU) No 15/2011 establishes the date of December 31, 2014 as the deadline for replacement of the mouse test with a LC-MS/MS method by the laboratories of the member states responsible for the monitoring of marine biotoxins. In order to facilitate the interpretation of the MBA during this transition period, we further evaluated the specificity and selectivity of this assay in comparison with a LC-MS/MS method in detecting lipophilic marine toxins on mussels of the middle Adriatic Sea. Data show a high percentage of false-positive results on MBA due to interference by yessotoxins. It was also possible to evaluate the toxic profile of the samples analyzed
The ϕ-meson leptonic widths, Γee and Γμμ, are obtained, respectively, from the e+e− forward–backward asymmetry and the μ+μ− cross section around the ϕ-mass energy. We find Γee=1.32±0.05±0.03 keV and ...ΓeeΓμμ=1.320±0.018±0.017 keV. These results, compatible with Γee=Γμμ, provide a precise test of lepton universality. Combining the two results gives Γℓℓ(ϕ)=1.320±0.023 keV.
Norovirus is the most prevalent causative agent of foodborne diseases. However, the detection of this virus in foods other than shellfish is often time-consuming and unsuccessful. The objective of ...this study is to compare PEG and ultrafiltration techniques for viral concentration in bivalve molluscs. An experiment with Coxsackie B5 and feline Calicivirus strain F is conduct to determine the efficiency of each virus concentration. Ultrafiltration technique is the most indicated.
From March 2007 to January 2008, a total of 170 pigs at slaughter were tested for Y. enterocolitica contamination in tonsils tissue. The animals came from 125 different farms located in four regions ...of Northern Italy. Y. enterocolitica was isolated from 19 out of 170 (11.2%) tonsils samples. The prevalent bio-serotype (68.4%) was 4/O:3, followed by bioserotypes 1A/O:8 (15.8%), 1A/O:5 (10.5%) and 4/O:8 (5.2%). Among bio-serotype 4/O:3, several strains possessed yadA, ail and ystA virulence genes.
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