Active alginate-based coatings with quercetin glycoside and complexes of hydroxyapatite/quercetin-glycoside were used to study the shelf life of fresh cut papaya stored at 6 °C. Hydroxyapatite was ...used as a carrier for the release of the bioactive compound. The parameters considered affecting the quality of the fruit during storage were weight loss, color, texture, sugars and volatile compounds. Active coatings with hydroxyapatite and quercetin glycoside proved a higher capacity to slow down the degradation phenomena studied, showing less weight loss, a lower reduction in glucose and fructose, as well as better firmness, than the other samples after 14 days of cold storage. Benzyl isothiocyanate, the characteristic odor compound of papaya fruit, ranged from approximately 10.0 μg/kg in fresh cut fruit to approximately 7.50 μg/kg in samples coated by alginate with hydroxyapatite/quercetin and 3.6 μg/kg in the fresh cut papaya without coating after 14 days of cold storage. The trials also indicated greater effectiveness of alginate coatings alone and with quercetin-glucoside in preserving the color of freshly cut papaya.
Summary
In this study, the soil effect on the chemical composition of red grapes from Vitis vinifera L. (cv Nero d'Avola) was studied. The soil effect was evaluated by considering the main soil ...chemical–physical parameters (e.g., texture, pH, total carbonates, cation exchange capacity, electric conductivity, organic matter, and mineral composition) of four vineyards located in the southwestern coast of Sicily (southern Italy). Grapes' quality in relation to different soils was investigated by studying some composition parameters and the (in)/homogeneity of ripeness of grapes at the harvest date. Results suggested that the soil greatly affected the quality of Nero d'Avola red grapes. The most impactful soil parameters were cation exchange capacity, electric conductivity, pH, and mineral composition (especially P and Mn content) that are directly involved into grapevine nutrition. Through multivariate statistical analysis, it was highlighted that the soil effect was remarkable within a specific vintage. However, when more vintages are considered simultaneously, the vintage effect (conceivably through the inter‐annual climatic variability) is the major factor determining grapes quality.
In this study, the soil effect on the chemical composition of Nero d'Avola red grapes was studied. Results suggested that the soil greatly affected the quality of Nero d'Avola grapes. The most impactful soil parameters were cation exchange capacity, electric conductivity, pH, and mineral composition that are directly involved into grapevine nutrition.
The aim of this study was to evaluate the effects of the prolonged post-fermentative maceration during the winemaking of Cabernet Sauvignon grapes in oak barrels and steel tanks and apply to this ...purpose a mathematical modelization to better and easily understand the dynamics and phenomena related to this process.
The study focused on the time series of phenolic and volatile compounds in wines during the maceration with solid parts and residual components of the alcoholic fermentation, only for anthocyanins and non-anthocyanin flavonoids two mathematical models were proposed.
The results showed that solid parts during the post-fermentative maceration phase provided a higher content of polyphenols and total flavonoids in oak barrels and steel tanks. The largest contents of polyphenols and flavonoids were observed in oak barrel trials. Among the anthocyanins in both trials, we have assisted in a decreasing trend in delphinidin-3 glucoside, cyanidin-3-glucoside, petunidin-3-glucoside and peonidin-3-glucoside, with a more accentuated process for the first two molecules in a barrique trial, also acetylated anthocyanins showed a decreasing trend more intense in the barrique trial.
Post-fermentative maceration improved the sensory characteristics in terms of intensity, complexity, and persistence at both olfactory and gustatory levels, especially in oak barrel vinification.
Four monofloral honeys, obtained from the Sicilian black bee by foraging on thistle, sulla, chestnut and eucalyptus, were studied. Results showed that the phenolic composition of chestnut honey was ...the highest (316 mg gallic acid equivalent GAE/kg), while that of sulla honey was the lowest (122 mg GAE/kg). Data confirmed a correlation between the total phenol content and colour intensity in chestnut honey, which was the darkest of the four samples. Sulla honey showed the highest antioxidant activity, while eucalyptus honey had the highest mineral content (K, Ca, Mg, and Na). Thistle honey showed the most intense floral and fruity aromas, as well as an intense -yellow colour. Principal component analysis showed the potential to discriminate different honeys in three different quadrants.
Loquat cultivation in Sicily is mainly based on nonnative cultivars and local ecotypes characterized by high nutraceutical value and appreciable physicochemical characteristics. Increased interest in ...commercial loquat production has increased the intention to provide premium quality loquat cultivars that include volatile substances capable of conditioning the sensorial properties and, therefore, the acceptability of fruits by consumers. This study determined the content of volatile compounds in nonnative and local loquat fruits grown in Sicily. Analyses were performed on five international cultivars and four local cultivars.
In this study, sup.1H-NMR spectroscopy coupled with chemometrics was applied to study the wine metabolome and to classify wines according to different grape varieties and different terroirs. By ...obtaining the metabolomic fingerprinting and profiling of the wines, it was possible to assess the metabolic biomarkers leading the classification (i.e., phenolic compounds, aroma compounds, amino acids, and organic acids). Moreover, information about the influence of the soil in shaping wine metabolome was obtained. For instance, the relationship between the soil texture and the content of amino acids and organic acids in wines was highlighted. The analysis conducted in this study allowed extraction of relevant spectral information not only from the most populated and concentrated spectral areas (e.g., aliphatic and carbinolic areas), but also from crowded spectral areas held by lowly concentrated compounds (i.e., polyphenols). This may be due to a successful combination between the parameters used for data reduction, preprocessing and elaboration. The metabolomic fingerprinting also allowed exploration of the H-bonds network inside the wines, which affects both gustatory and olfactory perceptions, by modulating the way how solutes interact with the human sensory receptors. These findings may have important implications in the context of food traceability and quality control, providing information about the chemical composition and biomolecular markers from a holistic point of view.
Gaucher disease (GD) diagnosis can be delayed due to non-specific symptoms and lack of awareness, leading to unnecessary procedures and irreversible complications. GAU-PED study aims to assess GD ...prevalence in a high-risk pediatric population and the presence, if any, of novel clinical or biochemical markers associated with GD.
DBS samples were collected and tested for β-glucocerebrosidase enzyme activity for 154 patients selected through the algorithm proposed by Di Rocco et al. Patients showing β-glucocerebrosidase activity below normal values were recalled to confirm the enzyme deficiency with the gold standard essay on cellular homogenate. Patients tested positive at the gold standard analysis were evaluated through GBA1 gene sequencing.
14 out of 154 patients were diagnosed with GD, with a prevalence of 9.09% (5.06-14.78%, CI 95%). Hepatomegaly, thrombocytopenia, anemia, growth delay/deceleration, elevated serum ferritin, elevated Lyso-Gb1 and chitotriosidase were significantly associated with GD.
GD prevalence in a pediatric population at high-risk appeared to be higher compared to high-risk adults. Lyso-Gb1 was associated with GD diagnosis. The algorithm proposed by Di Rocco et al. can potentially improve the diagnostic accuracy of pediatric GD, allowing the prompt start of therapy, aiming to reduce irreversible complications.
It has been demonstrated that HIV infection may affect the levels of thymidine kinase (TK) and deoxycytidine kinase (dCK) in peripheral blood mononuclear cells from HIV infected adults. The aim of ...this study was to examine the effect of HIV infection and/or antiretroviral therapy on the activity of the above enzymes in HIV-infected children. The results showed that an inter-individual variability in TK and dCK activities does exist in both HIV infected and uninfected children. TK and dCK levels in PBMC from HIV infected and non infected children did not significantly differ. Furthermore, the therapeutic regimen, including zidovudine, does not seem to affect TK activity.
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