In today’s increasingly frequent cultural exchanges between China and Japan, accurate and error-free Japanese translation has become an inevitable choice for cross-cultural communication. In this ...paper, based on twin neural network and attention mechanism, BiLSTM model is combined with sentence semantic similarity matching algorithm to construct a Japanese translation bias sentence semantic similarity model. The Japanese corpus data were collected and preprocessed by Python technology, and the Japanese translation corpus database was searched and counted using Wordsmith and AntConc tools. For the Japanese learners’ translation bias in the Japanese translation process, a comparative analysis was carried out in several aspects, such as end-of-sentence modal expressions, consecutive translations, and word frequency effects. The study results show that the difference in the frequency distribution of Japanese learners’ modal expressions is only 4.66% compared with that of native speakers of Japanese. Still, the difference between the two is significant at the 1% level, and the difference in the frequency of Japanese learners’ use of the modal expression “yes” is 56 sentences per 10,000 sentences. The frequency of Japanese learners’ use of successive expressions was 30.1 percentage points higher than that of native speakers. The neural semantic analysis method combined with the Japanese translation corpus can clarify the translation bias of Japanese learners in the process of Japanese translation, which can provide a reference for enhancing the translation quality of Japanese learning.
Janus colloids are functional particles consisting of two surfaces (or internal materials) with distinct physical or chemical properties in the same particle. Owing to their amphiphilic nature, Janus ...colloids composed of both hydrophilic and hydrophobic faces provide a powerful tool to generate functional surfaces and to manipulate the properties of interfaces. Amphiphilic Janus colloids have shown promising applications as particulate surfactants in oil/water separation, as interfacial compatibilizers in polymer blends, and as assembly blocks in robust coatings with unique wettability. In this Feature Article, we summarize recent advances in engineering interfaces by using Janus colloids.
Anoikis is a unique mode of apoptotic cell death that occurs consequentially to insufficient cell-matrix interactions. Resistance to anoikis is a critical contributor to tumor invasion and ...metastasis. The phenomenon is regulated by integrins, which upon engagement with components of the extracellular matrix (ECM) form adhesion complexes and the actin cytoskeleton drives the formation of cell protrusions used to adhere to ECM, directing cell migration. The epithelial-mesenchymal transition (EMT) confers stem cell properties and leads to acquisition of a migratory and invasive phenotype by causing adherens junction breakdown and circumventing anoikis in the tumor microenvironment. The investigation of drug discovery platforms for apoptosis-driven therapeutics identified several novel agents with antitumor action via reversing resistance to anoikis, inhibiting survival pathways and impacting the EMT landscape in human cancer. In this review, we discuss current evidence on the contribution of the anoikis phenomenon functionally linked to EMT to cancer metastasis and the therapeutic value of antitumor drugs that selectively reverse anoikis resistance and/or EMT to impair tumor progression toward the development/optimization of apoptosis-driven therapeutic targeting of metastatic disease.
Kongzhuang coal mine of Shanghai Datun Energy Resources Co., Ltd. is a typical deep coal mine in eastern China. The mining disturbance of working face in deep coal mine leads to the fracture and ...movement of overlying strata and the damage of the aquifer in overlying strata, thereby causing water inrush disaster and posing a serious threat to the safety production in coal mine. Therefore, the temporal and spatial evolution mechanism of the water-conducting fractured zone of overlying strata in the Kongzhuang coal mine is researched systematically in this paper. Especially, the hydro-geological conditions and mining conditions in the Kongzhuang coal mine are analyzed; on this basis, the fracture and movement of overlying strata are simulated by physical similarity simulation test, and the temporal and spatial evolution rule of overlying strata in the Kongzhuang coal mine is obtained. Besides, the development height of “two zones” is measured by double-end water shutoff detection method, and the risk assessment for the water inrush disaster of the coal seam roof is carried out in the Kongzhuang coal mine. The research achievements in this paper indicate that the water-conducting channel formed in the mining process of #7 coal seam is the most important water-filling channel. Quaternary aquifer water recharges the mine indirectly through the bedrock aquifer, and the sandy mudstone with large thickness is the key layer to control the development of a water-conducting fracture zone. Meanwhile, the height of the caving zone is 26.7 m, about 8.3 times of mining height, and the height of the fracture zone is 68.3 m, approximately equal to 16.26 of the mining height. The results of #1 and #2 borehole leakages in the double-end water shutoff detection method show that the height of the water-conducting fractured zone is 63.70 m-65.27 m, and the split-to-mining ratio is 15.17-15.54. The water inrush risk of the coal seam roof shows that most of the 7436 working face is in the transition zone, and a small area around the cutting hole of the working face is in the relatively dangerous zone. Therefore, the innovation of this paper is that the temporal and spatial evolution mechanism of the water-conducting fractured zone of overlying strata in the Kongzhuang coal mine is revealed, which provides the theoretical guidance for the prediction and prevention of water inrush disaster in the coal mine with the similar mining conditions.
Aflatoxin B1 (AFB1) pollutes foodstuffs and feeds, causing a food safety problem and seriously endangering human and animal health. Liver is the principal organ for AFB1 accumulation and ...biotransformation, during which AFB1 can cause acute and chronic liver damage, however, the specific mechanism is not completely clear. Mitochondria are the primary organelle of cellular bio-oxidation, providing 95% energy for liver to execute its multiple functions. Therefore, we speculated that mitochondrial dysfunction is involved in AFB1-induced liver injury. To verify the hypothesis, a total of eighty healthy male mice were randomly divided into four groups on average, and exposed with 0, 0.375, 0.75 and 1.5 mg/kg body weight AFB1 by intragastric administration for 30 d. The results displayed that AFB1 triggered liver injury accompanied by oxidative stress. AFB1 exposure also damaged mitochondria structure, decreased mitochondrial membrane potential (MMP), as well as increased cytoplasmic cytochrome c (Cyt-c) protein expression, Bax, p53, Caspase-3/9 protein and/or mRNA expression levels and terminal deoxynucleotidyl transferase-mediated 2'-deoxyuridine-5'-triphosphate (dUTP) nick end labeling (TUNEL) staining positive cells in mice liver. Meanwhile, AFB1 exposure elevated pyruvate content, inhibited tricarboxylic acid (TCA) cycle rate-limiting enzymes and electron transport chain (ETC) complexes I-V activities, disturbed ETC complexes I-V subunits mRNA expression levels and reduced adenosine triphosphate (ATP) level in mice liver. These results indicated that AFB1 destroyed mitochondrial structure, activated mitochondrion-dependent apoptosis and induced mitochondrial dysfunction. In addition, AFB1 disrupted mitochondrial biogenesis, presented as the abnormalities of protein and/or gene expression levels of voltage dependent anion channel protein 1 (VDAC1), peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α), nuclear respiratory factor 1 (Nrf1) and mitochondrial transcription factor A (Tfam). This may contribute to hepatic and mitochondrial lesions induced by AFB1. These results provide a new perspective for elucidating the mechanisms of AFB1 hepatotoxicity.
Aflatoxin B1 induced mice liver and mitochondrial lesions, which is associated with the mitochondrial biogenesis inhibition. Display omitted
•Aflatoxin B1 (AFB1) induced liver injury of mice.•Mitochondrial dysfunction is involved in AFB1-induced mice liver.•Mitochondrial biogenesis involved in AFB1-induced liver mitochondrial dysfunction.
A practical metal-free and additive-free approach for the synthesis of 6/7/8-membered oxacyclic ketone-fused isoxazoles/isoxazolines tetracyclic or tricyclic structures is reported through C
-H bond ...radical nitrile oxidation and the intramolecular cycloaddition of alkenyl/alkynyl-substituted aryl methyl ketones. This convenient approach enables the simultaneous formation of isoxazole/isoxazoline and 6/7/8-membered oxacyclic ketones to form polycyclic architectures by using
-butyl nitrite (TBN) as a non-metallic radical initiator and N-O fragment donor.
Cannabinoid has long been used for medicinal purposes. Cannabinoid signaling has been considered the therapeutic target for treating pain, addiction, obesity, inflammation, and other diseases. Recent ...studies have suggested that in addition to CB1 and CB2, there are non-CB1 and non-CB2 cannabinoid-related orphan GPCRs including GPR18, GPR55, and GPR119. In addition, CB1 and CB2 display allosteric binding and biased signaling, revealing correlations between biased signaling and functional outcomes. Interestingly, new investigations have indicated that CB1 is functionally present within the mitochondria of striated and heart muscles directly regulating intramitochondrial signaling and respiration.
In this review, we summarize the recent progress in cannabinoid-related orphan GPCRs, CB1/CB2 structure, Gi/Gs coupling, allosteric ligands and biased signaling, and mitochondria-localized CB1, and discuss the future promise of this research.
Abstract
Accumulating researches have confirmed that circRNA abnormal expression plays a prominent role in the progression of colorectal cancer (CRC). The role of circ_0000218 in CRC and its ...potential mechanism are not clear. In this study, real-time polymerase chain reaction (RT-PCR) was employed to measure the circ_0000218, miR-139-3p and RAB1A mRNA expression in CRC tissues and cells. Immunohistochemistry and western blot were conducted to determine the RAB1A expression in CRC tissues and cells, respectively. Colony formation assay and BrdU method were employed to monitor the effect of circ_0000218 on cell proliferation. Transwell assay was adopted to detect cell migration and invasion. Dual luciferase reporter assay and RNA immunoprecipitation assay were adopted to confirm the targeting relationship between circ_0000218 and miR-139-3p, miR-139-3p and RAB1A. We demonstrated that circ_0000218 was notably upregulated in CRC tissues and cell lines, and its high expression level was markedly linked to the increase of T staging and local lymph node metastasis. Circ_0000218 overexpression enhanced the proliferation and metastasis of CRC cells while knocking down circ_0000218 caused the opposite effects. We also observed that miR-139-3p was negatively regulated by circ_0000218, while RAB1A was positively regulated by it. Collectively, this study suggested that circ_0000218 upregulated RAB1A and promoted CRC proliferation and metastasis via sponging miR-139-3p.
A simple and efficient strategy was developed to fabricate CuO nanoparticles (CuO-NPs) with high surface area by the direct pyrolysis of a metal-organic gel (MOG) precursor for constructing versatile ...catalytic interfaces. Unexpectedly, the obtained CuO-NPs exhibited excellent electrocatalytic activity for glucose (Glu) oxidation reaction. The linear range of glucose was from 5 μM to 600 with the detection limit of 0.59 μM. Additionally, the CuO-NPs showed distinguished intrinsic peroxidase-mimicking activities, which can be further used as biomimetic nanozymes for sensitively and rapidly detecting cholesterol. A good linearity of cholesterol was performed in the range from 1 μM to 15 μM with the detection limit of 0.43 μM. The as-prepared CuO-NPs could provide a versatile catalytic platform for the application of electrochemical sensors and biomimetic enzyme catalytic systems. This study proved the high potential of MOG-derived nanostructured transition metal oxides (TMOs) with multiple complex functions.
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•Metal-organic gel (MOG) as a novel and potential precursor was utilized to synthesize porous CuO-NPs with high surface area.•The CuO-NPs exhibited excellent electrocatalytic and peroxidase-mimicking activities, which can construct versatile catalytic interfaces for promising applications in electrochemical sensors and biomimetic enzyme catalytic systems.•The novel idea revealed the high potential of MOG-derived nanostructured TMOs with multiple complex functions.
When the collapse column of overburden is disturbed by the working face, the grain loss in the karst collapse column occurs by the dissolution and corrosion of groundwater, thereby inducing the water ...inrush disaster. The test samples are prepared based on the fractal theory and the Talbol grading theory, and the seepage evolution law of fractured rock in collapse column under triaxial stress is studied, by employing the triaxial seepage test equipment. Besides, the seepage mechanics model of broken rock is established and calculated in the COMSOL Multiphysics, and the water-conducting channel under mass loss condition in the collapse column is further elucidated. The research results indicate that the loss ratio of mass is inversely proportional to the Talbol power index, and grain mass loss rate increases with the decrease of the Talbol power index. During the infiltration process, the evolution of pore structure is related with grain size distribution. With the increase of the Talbol power index, the overall porosity increases. Grain loss is an internal factor in seepage loss stability. Flow speed is accelerating, and seepage pathways are communicated with each other to induce the water inrush disaster.
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