Fine particulate matter is becoming a primary component of air pollution in China, damaging public health and economic growth. Although environmental regulation is an important instrument to control ...air pollution, studies on the effect of environmental regulation are mixed. Do environmental regulations help improve the air quality in urban centers, and what mechanism explains their effects in China? In order to explore the causal relationship between environmental regulations and air quality, the effectiveness of the New Ambient Air Quality Standards—primarily the monitoring of PM2.5—was evaluated using the Difference-in-Differences (DID) method to weaken the endogeneity problems. Our results suggest that the New Standards reduce the concentration of PM2.5 and emissions of SO2 in pilot cities in neither the short-term nor the long-term. Our heterogeneous analyses show that the monitoring efforts, pollution control efforts, and ownership structures can affect outcomes of New Standards significantly, and that they have different influences on various categories of pollutants. Our paper implies that the Chinese government should make more efforts beyond setting up environmental regulations.
•The average treatment effects in treated group of New Standards on SO2 and PM2.5 are zero.•Monitoring efforts and pollution investments can reduce SO2 in treated group, but will not affect PM2.5.•The effect of New Standards will be weakened for SO2 and PM2.5, in areas with high proportion of state-owned economy.•A reason for an ineffective environmental regulation is that its heterogeneous effects caused by regional features.
The effect of Ca and Zn in solid solution on the critical resolved shear stress (CRSS) of basal slip, tensile twinning and <c+a> pyramidal slip in Mg alloys has been measured through compression ...tests on single crystal micropillars with different orientations. The solute atoms increased the CRSS for basal slip to ~ 13.5 MPa, while the CRSS for pyramidal slip was lower than 85 MPa, reducing significantly the plastic anisotropy in comparison with pure Mg. Moreover, the CRSSs for twin nucleation and growth were very similar (~ 37 MPa) and the large value of the CRSS for twin growth hindered the growth of twins during thermo-mechanical processing. Finally, evidence of prismatic slip and cross-slip between basal and prismatic dislocations was found. It is concluded that the reduction of plastic anisotropy, the activation of different slip systems and cross-slip and the weak basal texture promoted by the large CRSS for twin growth are responsible for the improved ductility and formability of Mg-Ca-Zn alloys.
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Resilience has begun to receive attention in entrepreneurship research. However, most studies focus on organizational and individual resilience; little is known about team resilience in the ...entrepreneurship field. To fill the gap, this study explores team resilience and its formation and function in a specific context: new venture teams (NVTs). Conceptualizing team resilience as a second-order emergent state with first-order dimensions being resilience-efficacious beliefs and resilience-adaptive capacity, this study articulates the role of behavioral integration in cultivating team resilience and tests the effect of team resilience on NVT performance. Furthermore, a double-edged sword effect of affective integration is proposed: it strengthens the link between behavioral integration and team resilience but weakens the tie between team resilience and performance. Survey data collected from 488 entrepreneurs in 110 NVTs lend support to our hypotheses. These findings add to the knowledge of team resilience in a unique entrepreneurship setting, expand our understanding of NVT effectiveness, and provide implications to NVTs in terms of resilience building and team climate management.
Complex diseases, such as cancer, are often associated with aberrant gene expression at both the transcriptional and post-transcriptional level. Over the past several years, competing endogenous RNAs ...(ceRNAs) have emerged as an important class of post-transcriptional regulators that alter gene expression through a miRNA-mediated mechanism. Recent studies in both solid tumors and hematopoietic malignancies showed that ceRNAs have significant roles in cancer pathogenesis by altering the expression of key tumorigenic or tumor-suppressive genes. Characterizing the identity, function, and mechanism of the ceRNAs will not only further our fundamental understanding of RNA-mediated cancer pathogenesis, but may also shed light on the development of new RNA-based therapeutic strategies for treating cancer.
This systematic review and meta-analysis examined the associations between obesity and puberty timing based on scientific evidence. Eight electronic databases were searched up to February 2017 for ...eligible studies, and two reviewers screened the articles and extracted the data independently. A total of 11 cohort studies with 4841 subjects met the inclusion criteria. Compared with the group of normal-weight girls, the obese group had more girls with menarche (RR: 1.87, 95% CI: 1.59-2.19, 2 studies). The number of girls with early puberty was significantly higher in the obese group than the normal weight group (RR: 2.44, 95% CI: 1.32-4.52, 5 studies). However, no differences were detected between girls who were obese or normal weight at age of menarche (WMD: -0.53 years, 95% CI: -1.24-0.19, 2 studies). There is no consistent result in the relationship between obesity and timing of pubertal onset in boys. Obesity may contribute to early onset of puberty in girls, while in boys, there is insufficient data. Given the limited number of cohort studies included in this meta-analysis, high-quality studies with strong markers of puberty onset, as well as standardized criteria for defining obesity are needed.
Mounting evidence suggests that long noncoding RNAs (lncRNAs) can function as microRNA sponges and compete for microRNA binding to protein-coding transcripts. However, the prevalence, functional ...significance and targets of lncRNA-mediated sponge regulation of cancer are mostly unknown. Here we identify a lncRNA-mediated sponge regulatory network that affects the expression of many protein-coding prostate cancer driver genes, by integrating analysis of sequence features and gene expression profiles of both lncRNAs and protein-coding genes in tumours. We confirm the tumour-suppressive function of two lncRNAs (TUG1 and CTB-89H12.4) and their regulation of PTEN expression in prostate cancer. Surprisingly, one of the two lncRNAs, TUG1, was previously known for its function in polycomb repressive complex 2 (PRC2)-mediated transcriptional regulation, suggesting its sub-cellular localization-dependent function. Our findings not only suggest an important role of lncRNA-mediated sponge regulation in cancer, but also underscore the critical influence of cytoplasmic localization on the efficacy of a sponge lncRNA.
Nowadays, improving the traffic safety of visually impaired people is a topic of widespread concern. To help avoid the risks and hazards of road traffic in their daily life, we propose a wearable ...device using object detection techniques and a novel tactile display made from shape-memory alloy (SMA) actuators. After detecting obstacles in real-time, the tactile display attached to a user’s hands presents different tactile sensations to show the position of the obstacles. To implement the computation-consuming object detection algorithm in a low-memory mobile device, we introduced a slimming compression method to reduce 90% of the redundant structures of the neural network. We also designed a particular driving circuit board that can efficiently drive the SMA-based tactile displays. In addition, we also conducted several experiments to verify our wearable assistive device’s performance. The results of the experiments showed that the subject was able to recognize the left or right position of a stationary obstacle with 96% accuracy and also successfully avoided collisions with moving obstacles by using the wearable assistive device.
Alternative RNA splicing plays an important role in cancer. To determine which factors involved in RNA processing are essential in prostate cancer, we performed a genome-wide CRISPR/Cas9 knockout ...screen to identify the genes that are required for prostate cancer growth. Functional annotation defined a set of essential spliceosome and RNA binding protein (RBP) genes, including most notably heterogeneous nuclear ribonucleoprotein L (HNRNPL). We defined the HNRNPL-bound RNA landscape by RNA immunoprecipitation coupled with next-generation sequencing and linked these RBP–RNA interactions to changes in RNA processing. HNRNPL directly regulates the alternative splicing of a set of RNAs, including those encoding the androgen receptor, the key lineage-specific prostate cancer oncogene. HNRNPL also regulates circular RNA formation via back splicing. Importantly, both HNRNPL and its RNA targets are aberrantly expressed in human prostate tumors, supporting their clinical relevance. Collectively, our data reveal HNRNPL and its RNA clients as players in prostate cancer growth and potential therapeutic targets.
The androgen receptor (AR) is a key factor that regulates the behavior and fate of prostate cancer cells. The AR-regulated network is activated when AR binds enhancer elements and modulates specific ...enhancer–promoter looping. Kallikrein-related peptidase 3 (KLK3), which codes for prostate-specific antigen (PSA), is a well-known AR-regulated gene and its upstream enhancers produce bidirectional enhancer RNAs (eRNAs), termed KLK3e. Here, we demonstrate that KLK3e facilitates the spatial interaction of the KLK3 enhancer and the KLK2 promoter and enhances long-distance KLK2 transcriptional activation. KLK3e carries the core enhancer element derived from the androgen response element III (ARE III), which is required for the interaction of AR and Mediator 1 (Med1). Furthermore, we show that KLK3e processes RNA-dependent enhancer activity depending on the integrity of core enhancer elements. The transcription of KLK3e was detectable and its expression is significantly correlated with KLK3 (R ² = 0.6213, P < 5 × 10 ⁻¹¹) and KLK2 (R ² = 0.5893, P < 5 × 10 ⁻¹⁰) in human prostate tissues. Interestingly, RNAi silencing of KLK3e resulted in a modest negative effect on prostate cancer cell proliferation. Accordingly, we report that an androgen-induced eRNA scaffolds the AR-associated protein complex that modulates chromosomal architecture and selectively enhances AR-dependent gene expression.
Mycoplasmas, the smallest bacteria lacking a cell wall, can cause various diseases in both humans and animals. Mycoplasmas harbor a variety of virulence factors that enable them to overcome numerous ...barriers of entry into the host; using accessory proteins, mycoplasma adhesins can bind to the receptors or extracellular matrix of the host cell. Although the host immune system can eradicate the invading mycoplasma in most cases, a few sagacious mycoplasmas employ a series of invasion and immune escape strategies to ensure their continued survival within their hosts. For instance, capsular polysaccharides are crucial for anti-phagocytosis and immunomodulation. Invasive enzymes degrade reactive oxygen species, neutrophil extracellular traps, and immunoglobulins. Biofilm formation is important for establishing a persistent infection. During proliferation, successfully surviving mycoplasmas generate numerous metabolites, including hydrogen peroxide, ammonia and hydrogen sulfide; or secrete various exotoxins, such as community-acquired respiratory distress syndrome toxin, and hemolysins; and express various pathogenic enzymes, all of which have potent toxic effects on host cells. Furthermore, some inherent components of mycoplasmas, such as lipids, membrane lipoproteins, and even mycoplasma-generated superantigens, can exert a significant pathogenic impact on the host cells or the immune system. In this review, we describe the proposed virulence factors in the toolkit of notorious mycoplasmas to better understand the pathogenic features of these bacteria, along with their pathogenic mechanisms.
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