UV-A radiation affects skin homeostasis by promoting oxidative distress. Endogenous photosensitizers in the dermis and epidermis of human skin absorb UV-A radiation forming excited states (singlet ...and triplet) and reactive oxygen species (ROS) producing oxidized compounds that trigger biological responses. The activation of NF-kB induces the expression of pro-inflammatory cytokines and can intensify the generation of ROS. However, there is no studies evaluating the cross talks between inflammatory stimulus and UV-A exposure on the levels of redox misbalance and inflammation. In here, we evaluated the effects of UV-A exposure on J774 macrophage cells previously challenged with LPS in terms of oxidative distress, release of pro-inflammatory cytokines, and activation of regulated cell death pathways. Our results showed that LPS potentiates the dose-dependent UV-A-induced oxidative distress and cytokine release, in addition to amplifying the regulated (autophagy and apoptosis) and non-regulated (necrosis) mechanisms of cell death, indicating that a previous inflammatory stimulus potentiates UV-A-induced cell damage. We discuss these results in terms of the current-available skin care strategies.
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•UVA and LPS potentiates the generation of ROS and the release of pro-inflammatory cytokines.•UV-A and LPS potentiates macrophage death orchestrated by redox imbalance.•UV-A exposure and inflammatory responses potentiates each other effects.•People should avoid extensive sun exposure even with the partial protection offered by sun blocker agents.
Porcine circovirus-2 (PCV-2) infection is currently considered an important disease of swine. The pathogenic agent was first described in Brazil in 2000. This study detected the PCV-2 DNA in four ...Brazilian pig tissues collected between 1978 and 1979. This observation is the oldest description of this virus in Brazil.
Quercetin as a shuttle for labile iron Baccan, Mayara Marinovic; Chiarelli-Neto, Orlando; Pereira, Regina Mara Silva ...
Journal of inorganic biochemistry,
02/2012, Volume:
107, Issue:
1
Journal Article
Peer reviewed
The antioxidant activity of flavonoids may involve their ability to complex body iron in non-redox-active forms. In this study, it was found that the catechol flavonoids rutin and quercetin are able ...to suppress redox-active labile plasma iron (LPI) in both buffered solution and in iron-overloaded sera. Both flavonoids are effective in loading the metal into the iron-transport protein transferrin. Iron derivatives of quercetin and rutin are able to permeate cell membranes, however, only free quercetin is able to gain access to the cytosol and decrease intracellular labile iron pools. These results suggest that the antioxidant activity of quercetin may be dependent on its ability to shuttle labile iron from cell compartments followed by its transfer to transferrin.
Quercetin may remove labile iron from iron-overloaded cells and transfer it without generating oxidative stress to other high-affinity molecules (e.g., transferrin).
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► Catechol flavonoids rutin and quercetin decrease labile iron in plasma and solution. ► They also transfer iron to high the affinity molecules transferrin and calcein. ► Quercetin (but not rutin) can decrease intracellular labile iron pools. ► Quercetin may be used in iron-redistribution therapy.
The effects of UV radiation on skin cells are well known, while the effect of other fractions of the solar spectrum remains ignored. Recently, we observed that both neonatal primary normal human ...keratinocyte (NHK) and in immortalized human keratinocytes (HaCaT) cells exposed to UVA and after exposure to visible light had reduction in cell viability, increasing level of redox misbalance as monitored by the increase in DCF fluorescence, and the generation of singlet oxygen. We also detected strand breaks of nuclear DNA and premutagenic Fpg and Endo III-sensitive DNA lesions by Comet assay. Our data showed that UVA radiation (λ= 366 nm, 12 J.cm-2) generates lipofuscin, which is an autofluorescent visible-photosensitizer and a subproduct of incomplete lysosomal digestion of oxidized biomolecules, organelles, and membranes. Visible light photosensitizes lipofuscin inducing photooxidatives processes through the generation of several oxidizing species, which can damage DNA and generate premutagenic lesions, as 8-oxo-dG. Lipofuscinogenesis was also shown to occur more effectively through specific oxidative damages in lysosomes and mitochondria by with photosensitization of 1,9-dimethyl methylene blue (DMMB) 10 nM with red light (λ= 633 nm, 11 J.cm-2). We have then characterized the lipofuscin from human skin keratinocytes by fluorescence lifetime imaging and fluorescence spectroscopy (fluorescence lifetime = 2 ns; excitation at 450 nm and emission at 640 nm). Concluding, UVA and visible light can be dangerous to skin health, raising the importance of not only protecting ourselves against UV radiation, but also against visible light.
Infecção do Trato Urinário (ITU) é uma doença bacteriana que acomete principalmente o sexo feminino com maior prevalência em crianças e idosos. Os antibióticos possuem uma capacidade de inibir o ...crescimento bem como destruir as bactérias que causam a ITU. O uso de antibióticos sem condução do antibiograma tem aumentado o número de isolados resistentes. O foco deste estudo foi investigar a incidência e a resistência aos antibióticos das bactérias causadoras da ITU em hospitais de Colatina. Laudos da urocultura dos pacientes hospitalizados no período de 2015 a 2019 foram investigados. Faixa etária, sexo, agentes etiológicos mais freqüentes e resistência aos antibióticos foram analisados. A avaliação estatística foi realizada pelo programa Origin 8,0 a p < 0,05. Os resultados mostraram maior incidência de ITU em crianças e idosos com acometimento maior em pacientes do sexo feminino (56%). Klebsiella pneumoniae (31%), Escherichia coli (29%), Enterococcus sp (20%), Pseudomonas aeruginosa (7%), Proteus (7%) e Stapylococcus coagulase negativa (6%) foram as bactérias mais prevalentes. Klebsiella pneumoniae mostrou-se resistente em 41% dos antibióticos administrados, Stapylococcus coagulase negativa 38%, Pseudomonas aeruginosa 30%, Proteus 29%, Escherichia coli 21% e Enterococcus sp 19%. Amicacina, gentamicina, imipenem e vancomicina foram os antibióticos mais eficazes para o tratamento da ITU. Pacientes internados em hospitais de Colatina com ITU apresentaram resistência bacteriana em torno de 40% para a maioria dos antibióticos administrados. Portanto faz-se necessário o uso do antibiograma para evitar o aumento da resistência bacteriana nos ambientes hospitalares de Colatina--ES. Palavras-chave: Infecção do trato urinário; Resistência microbiana; Antibiótico; Antibiograma. Urinary tract infection (UTI) is a bacterial disease that mainly affects females, with a higher prevalence in children and the elderly. Antibiotics have the ability to inhibit growth and destroy bacteria that cause UTI. The use of antibiotics without conducting antibiograms increased the number of resistant isolates. The focus of this study was to investigate the incidence and antibiotic resistance of bacteria that cause UTIs in Colatina hospitals. Uroculture reports of patients hospitalized between 2015 and 2019 were investigated. Statistical evaluation was performed using Origin 8.0 at p < 0.05. The results showed a higher incidence of UTI in children and the elderly, with a greater number of female patients (56%). Klebsiella pneumoniae (31%), Escherichia coli (29%), Enterococcus sp. (20%), Pseudomonas aeruginosa (7%), Proteus (7%), and Staphylococcus sp. (6%) were the most prevalent. Klebsiella pneumoniae was resistant to 41% of the antibiotics administered. The other bacteria showed 38% resistance to Staphylococcus sp., 30% to Pseudomonas aeruginosa, 29% to Proteus, 21% to Escherichia coli, and 19% to Enterococcus sp. Amikacin, gentamicin, imipenem, and vancomycin were the most effective antibiotics for the treatment of UTIs. Patients admitted to Colatina hospitals with UTI showed a bacterial resistance of approximately 40% for most antibiotics administered. Therefore, it is necessary to use antibiograms to avoid increasing bacterial resistance in hospital environments in Colatina, ES. Keywords: Urinary tract infection; Microbial resistance; Antibiotic Antibiogram.
RESUMO: A água é um direito do ser humano, que além de ser fornecida em quantidade adequada, deve ser qualitativamente favorável ao consumo. Objetivou-se neste estudo mensurar os impactos na ...população ribeirinha da cidade Colatina–ES provocados pelo desprendimento de rejeitos de mineração no Rio Doce, provenientes da cidade de Mariana–MG. Os métodos utilizados para o projeto foram embasados em uma pesquisa teórica seguida de um trabalho em campo de caráter longitudinal, quantitativo e não probabilístico. A abordagem, no âmbito epidemiológico, demonstrou aumento expressivo na incidência de sinais e sintomas prodrômicos de patologias, como a diarreia, febre e afecções de pele e fâneros que obtiveram evolução aproximada de 172,7%, 133,3% e 35,3%, respectivamente. Tais parâmetros estão intimamente associadas as consequências do desastre ambiental, pelas alterações do meio ambiente, interrupção do fornecimento de água, contaminação hídrica, dentre outras. Notou-se necessidade de redefinir temáticas de educação em saúde pelas instituições governamentais para o manejo de qualidade da água. Sintomas como febre, diarreia, e alterações de pele tiveram elevação das taxas associadas com efeitos adversos no âmbito biológico, psicológico, social e econômico.
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