The effects of psycholinguistic variables are critical to the evaluation of theories about the cognitive reading system. However, reading research has tended to focus on the impact of key variables ...on average performance. We report the first investigation examining variation in psycholinguistic effects across the life span, from childhood into old age. We analyzed the performance of a sample of 535 readers, aged 8-83 years in lexical decision and pronunciation tasks. Our findings show that the effects on reading of two key variables, frequency and AoA, decrease in size with increasing age over the life span. We observed the systematic modulation by age and reading ability of these and other psycholinguistic effects alongside a global U-shaped effect of age. Diffusion model analyses suggest that developmental speed-up in decision responses can be attributed to the increasing quality of evidence accumulation in reaction to words, while the ageing-related slowing can be attributed to decreasing efficiency of stimulus encoding or response execution processes. An analysis of spoken response durations furnishes a consistent picture in which the slowing of pronunciation responses with age can be attributed to slowing articulatory processes. We think our findings can be explained by theoretical accounts that incorporate learning as the basis for the development of structure in the reading system. However, an adequate theory shall have to include assumptions about both developmental learning and later ageing. Our results warrant a life span theory of reading.
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CEKLJ, FFLJ, NUK, ODKLJ, PEFLJ
Nephelometric turbidity, a measure of light scattering by particles suspended in water, is commonly used for indicating water clarity or suspended particulate matter (SPM) concentration. Different ...turbidity sensors have long been known to respond differently to the same suspensions. Design standards have been introduced to improve comparability of turbidity sensors, notably the ISO‐7027 standard adopted by a number of manufacturers. We compared six ISO‐7027‐compliant nephelometers in river silt, kaolinite (layer clay) and algae‐laden pond water, with rigorous tank experiments over a wide (100‐fold) concentration range. The responses of four different field‐type (in situ) and two cuvette instruments, all calibrated to the same freshly made formazin standards, were very strongly linearly correlated, but ranged about twofold in magnitude. Apparently, even sensors meeting the same design standard (ISO‐7027) cannot be relied on to output numerically similar formazin nephelometric unit (FNU) values. This weak numerical comparability highlights the futility of treating turbidity as an absolute quantity, for example in environmental standards or studies of fine SPM effects on aquatic life. Indeed, reporting of turbidity in informal units such as FNU is best avoided. Turbidity records should be converted, by site‐specific calibrations, to quantities of ultimate interest such as SPM concentration or total phosphorus. For performance monitoring of field nephelometers, we advocate routine site‐specific calibration, not to formazin, but to the light beam attenuation coefficient (beam‐c; units: m−1). Beam‐c is a proper (SI) physical quantity that can be precisely measured by beam transmissometry, as in our experiments, and is accurately convertible to visual clarity.
Six different nephelometric turbidity sensors, all‐compliant with the international design standard (ISO‐7027), varied two‐fold in response (FNUs) in tank tests with three optically contrasting suspended materials over a wide range of concentrations. This ‘numerical ambiguity’ of turbidity reveals it to be a flawed metric. A superior metric, the light beam attenuation coefficient (in SI units: m−1), was used as the optical reference in our experiments—and has the major practical advantage that it controls the visual clarity of waters.
Salmonellosis is the 2nd most common cause of human bacterial food poisoning and can be acquired from meat or eggs, either via direct consumption or cross‐contamination in the kitchen. The European ...Commission has set the criteria to control Salmonella infections within the poultry sector and it is proposed that the swine sector should follow. Pork is considered, after eggs, the major source of infection in humans in the EU, with Salmonella typhimurium, including monophasic strains, being frequently implicated. Good control measures at the farm level are likely to correspond with lower prevalence of Salmonella infection and, subsequently, a reduction of cross‐contamination of carcasses processed at the slaughterhouse and a reduction in human salmonellosis. This review focuses on biosecurity measures in pig farms that can help to control important pig diseases at the same time as reducing the within‐herd prevalence of Salmonella. This information is likely to provide an economic incentive for farmers to apply improved general standards of farm biosecurity and hygiene management that would have a positive impact in food safety.
Recently emerged
Infantis strains carrying resistance to several commonly used antimicrobials have been reported from different parts of the globe, causing human cases of salmonellosis and with ...occurrence reported predominantly in broiler chickens. Here, we performed phylogenetic and genetic clustering analyses to describe the population structure of 417
Infantis originating from multiple European countries and the Americas collected between 1985 and 2019. Of these, 171 were collected from 56 distinct premises located in England and Wales (E/W) between 2009 and 2019, including isolates linked to incursions of multidrug-resistant (MDR) strains from Europe associated with imported poultry meat. The analysis facilitated the comparison of isolates from different E/W sources with isolates originating from other countries. There was a high degree of congruency between the outputs of different types of population structure analyses revealing that the E/W and central European (Germany, Hungary, and Poland) isolates formed several disparate groups, which were distinct from the cluster relating to the United States (USA) and Ecuador/Peru, but that isolates from Brazil were closely related to the E/W and the central European isolates. Nearly half of the analysed strains/genomes (194/417) harboured the IncFIB(pN55391) replicon typical of the "parasitic" pESI-like megaplasmid found in diverse strains of
Infantis. The isolates that contained the IncFIB(pN55391) replicon clustered together, despite originating from different parts of the globe. This outcome was corroborated by the time-measured phylogeny, which indicated that the initial acquisition of IncFIB(pN55391) likely occurred in Europe in the late 1980s, with a single introduction of IncFIB(pN55391)-carrying
Infantis to the Americas several years later. Most of the antimicrobial resistance (AMR) genes were identified in isolates that harboured one or more different plasmids, but based on the short-read assemblies, only a minority of the resistance genes found in these isolates were identified as being associated with the detected plasmids, whereas the hybrid assemblies comprising the short and long reads demonstrated that the majority of the identified AMR genes were associated with IncFIB(pN55391) and other detected plasmid replicon types. This finding underlies the importance of applying appropriate methodologies to investigate associations of AMR genes with bacterial plasmids.
•We develop an integrated approach to energy sector and economywide modeling.•Exploitation of regional hydropower potential at scale is analyzed.•A regional energy strategy, anchored in hydropower, ...represents a potentially attractive option.
We develop a sequential approach to link a bottom-up energy sector model to a detailed dynamic general equilibrium model of South Africa. The approach is designed to simultaneously address the shortcomings and maintain the attractive features of detailed energy sector and general equilibrium models. It also reflects common country-level energy planning processes. We illustrate the capabilities of this integrated bottom-up approach by analyzing the implications of (i) a carbon tax, (ii) liberalization of import supply restrictions in order to exploit regional hydropower potential, and (iii) a combined policy where both carbon taxes and import liberalization are pursued. For the combined scenario, our results suggest substantial emissions reductions relative to Baseline at essentially no cost to economic growth but about a one percent reduction in employment. We conclude that a regional energy strategy, anchored in hydropower, represents a potentially inexpensive approach to decarbonizing the South African economy. The strategy also has political economy attractions in that the combined approach reduces the burden of adjustment of politically sensitive sectors.
DNA methylation is the most stable type of epigenetic modification modulating the transcriptional plasticity of mammalian genomes. Using bisulfite DNA sequencing, we report high-resolution ...methylation profiles of human chromosomes 6, 20 and 22, providing a resource of about 1.9 million CpG methylation values derived from 12 different tissues. Analysis of six annotation categories showed that evolutionarily conserved regions are the predominant sites for differential DNA methylation and that a core region surrounding the transcriptional start site is an informative surrogate for promoter methylation. We find that 17% of the 873 analyzed genes are differentially methylated in their 5' UTRs and that about one-third of the differentially methylated 5' UTRs are inversely correlated with transcription. Despite the fact that our study controlled for factors reported to affect DNA methylation such as sex and age, we did not find any significant attributable effects. Our data suggest DNA methylation to be ontogenetically more stable than previously thought.
The term schizotypy refers to a group of stable personality traits with attributes similar to symptoms of schizophrenia, usually classified in terms of positive, negative or cognitive disorganization ...symptoms. The observation of increased spreading of semantic activation in individuals with schizotypal traits has led to the hypothesis that thought disorder, one of the characteristics of cognitive disorganization, stems from semantic disturbances. Nevertheless, it is still not clear under which specific circumstances (i.e., automatic or controlled processing, direct or indirect semantic relation) schizotypy affects semantic priming or whether it does affect it at all. We conducted two semantic priming studies with volunteers varying in schizotypy, one with directly related prime-target pairs and another with indirectly related pairs. Our participants completed a lexical decision task with related and unrelated pairs presented at short (250 ms) and long (750 ms) stimulus onset asynchronies (SOAs). Then, they responded to the brief versions of the Schizotypal Personality Questionnaire and the Oxford-Liverpool Inventory of Feelings and Experiences, both of which include measures of cognitive disorganization. Bayesian mixed-effects models indicated expected effects of SOA and semantic relatedness, as well as an interaction between relatedness and directness (greater priming effects for directly related pairs). Even though our analyses demonstrated good sensitivity, we observed no influence of cognitive disorganization over semantic priming. Our study provides no compelling evidence that schizotypal symptoms, specifically those associated with the cognitive disorganization dimension, are rooted in an increased spreading of semantic activation in priming tasks.
Enteritidis is a major cause of salmonellosis worldwide and more than 80% of outbreaks investigated in Europe have been associated with the consumption of poorly cooked eggs or foods containing raw ...eggs. Vaccination has been proven to be one of the most important measures to control
Enteritidis infections in poultry farms as it can decrease colonization of the reproductive organs and intestinal tract of laying hens, thereby reducing egg contamination. Differentiation of live vaccine from field or wild type
. Enteritidis isolates in poultry is essential for monitoring of veterinary isolates and targetting control actions. Due to decreasing costs, whole genome sequencing (WGS) is becoming a key tool for characterization of
isolates, including vaccine strains. Using WGS we described the genetic changes in the live attenuated Salmovac 440 and AviPro SALMONELLA VAC E vaccine strains and developed a method for differentiation from the wildtype
. Enteritidis strains. SNP analysis confirmed that streptomycin resistance was associated with a Lys43Arg missense mutation in the
L gene whilst 3 missense mutations in
and 1 missense mutation in
confer erythromycin sensitivity in AviPro SALMONELLA VAC E. Further mutations Arg242His in
and Gly236Arg in the
gene were related to adenine and histidine dependencies in Salmovac 440. Unique SNPs were used to construct a database of variants for differentiation of vaccine from the wildtype isolates. Two fragments from each vaccine were represented in the database to ensure high accuracy. Each of the two selected Salmovac 440 fragments differed by 6 SNPs from the wildtype and the AviPro SALMONELLA VAC E fragments differed by 4 and 6 SNPs, respectively. CD-hit software was applied to cluster similar fragments that produced the best fit output when searched with SRST2. The developed vaccine differentiation method was tested with 1,253 genome samples including field isolates of Salmovac 440 (
= 51), field isolates of AviPro SALMONELLA VAC E (
= 13),
. Gallinarum (
= 19),
. Pullorum (
= 116),
. Enteritidis (
= 244),
. Typhimurium (
= 810) and achieved 100% sensitivity and specificity.
There have been more than 425 million COVID-19 infections worldwide. Post-COVID illness has become a common, disabling complication of this infection. Therefore, it presents a significant challenge ...to global public health and economic activity.
Comprehensive clinical assessment (symptoms, WHO performance status, cognitive testing, CPET, lung function, high-resolution CT chest, CT pulmonary angiogram and cardiac MRI) of previously well, working-age adults in full-time employment was conducted to identify physical and neurocognitive deficits in those with severe or prolonged COVID-19 illness.
205 consecutive patients, age 39 (IQR30.0-46.7) years, 84% male, were assessed 24 (IQR17.1-34.0) weeks after acute illness. 69% reported ≥3 ongoing symptoms. Shortness of breath (61%), fatigue (54%) and cognitive problems (47%) were the most frequent symptoms, 17% met criteria for anxiety and 24% depression. 67% remained below pre-COVID performance status at 24 weeks. One third of lung function tests were abnormal, (reduced lung volume and transfer factor, and obstructive spirometry). HRCT lung was clinically indicated in <50% of patients, with COVID-associated pathology found in 25% of these. In all but three HRCTs, changes were graded 'mild'. There was an extremely low incidence of pulmonary thromboembolic disease or significant cardiac pathology. A specific, focal cognitive deficit was identified in those with ongoing symptoms of fatigue, poor concentration, poor memory, low mood, and anxiety. This was notably more common in patients managed in the community during their acute illness.
Despite low rates of residual cardiopulmonary pathology, in this cohort, with low rates of premorbid illness, there is a high burden of symptoms and failure to regain pre-COVID performance 6-months after acute illness. Cognitive assessment identified a specific deficit of the same magnitude as intoxication at the UK drink driving limit or the deterioration expected with 10 years ageing, which appears to contribute significantly to the symptomatology of long-COVID.
The growing threat of antimicrobial resistance worldwide has led to an increasing concern in the human, veterinary, and environmental fields, highlighting the need for strategies to effectively ...control bacterial contamination. Correct biosecurity practices, including the appropriate use of disinfectants, play a crucial role in controlling bacterial contamination. This study aimed to verify whether the recommended concentrations defined according to the Defra General Orders concentration (GO, published by the UK Department for Environment, Food and Rural Affairs’ disinfectant-approval scheme) of five commercial disinfectant preparations (peroxygen-based, phenol-based, two halogen-releasing agents, and glutaraldehyde/quaternary ammonium compound-based; disinfectants A to E, respectively) were sufficient to inhibit growth and inactivate selected bacterial strains, including some that carry known phenotypic patterns of multidrug resistance. The effectiveness of each disinfectant was expressed as the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values, determined by the broth-microdilution method. The results indicate that the type of disinfectant and its concentration influence the inhibitory and bactericidal efficacy. The glutaraldehyde/quaternary ammonium compound-based (disinfectant D) and chlorocresol-based products (disinfectant B) were the most effective, and the GO concentration was bactericidal in all the strains tested. The efficacy of the other compounds varied, depending on the bacterial species tested. The GO concentrations were at least able to inhibit the bacterial growth in all the products and bacterial strains tested. A greater tolerance to the compounds was observed in the strains of E. coli with multidrug-resistance profiles compared to the strains that were sensitive to the same antimicrobials.
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