The existence of temperature gradients within eukaryotic cells has been postulated as a source of natural convection in the cytoplasm, i.e. bulk fluid motion as a result of ...temperature-difference-induced density gradients. Recent computations have predicted that a temperature differential of DELTAT almost equal to 1 K between the cell nucleus and the cell membrane could be strong enough to drive significant intracellular material transport. We use numerical computations and theoretical calculations to revisit this problem in order to further understand the impact of temperature gradients on flow generation and advective transport within cells. Surprisingly, our computations yield flows that are an order of magnitude weaker than those obtained previously for the same relative size and position of the nucleus with respect to the cell membrane. To understand this discrepancy, we develop a semi-analytical solution of the convective flow inside a model cell using a bi-spherical coordinate framework, for the case of an axisymmetric cell geometry (i.e. when the displacement of the nucleus from the cell centre is aligned with gravity). We also calculate exact solutions for the flow when the nucleus is located concentrically inside the cell. The results from both theoretical analyses agree with our numerical results, thus providing a robust estimate of the strength of cytoplasmic natural convection and demonstrating that these are much weaker than previously predicted. Finally, we investigate the ability of the aforementioned flows to redistribute solute within a cell. Our calculations reveal that, in all but unrealistic cases, cytoplasmic convection has a negligible contribution toward enhancing the diffusion-dominated mass transfer of cellular material.
Cellular motility is a key function guiding microbial adhesion to interfaces, which is the first step in the formation of biofilms. The close association of biofilms and bioremediation has prompted ...extensive research aimed at comprehending the physics of microbial locomotion near interfaces. We study the dynamics and statistics of microorganisms in a 'floating biofilm',
i.e.
, a confinement with an air-liquid interface on one side and a liquid-liquid interface on the other. We use a very general mathematical model, based on a multipole representation and probabilistic simulations, to ascertain the spatial distribution of microorganisms in films of different viscosities. Our results reveal that microorganisms can be distributed symmetrically or asymmetrically across the height of the film, depending on their morphology and the ratio of the film's viscosity to that of the fluid substrate. Long-flagellated, elongated bacteria exhibit stable swimming parallel to the liquid-liquid interface when the bacterial film is less viscous than the underlying fluid. Bacteria with shorter flagella on the other hand, swim away from the liquid-liquid interface and accumulate at the free surface. We also analyze microorganism dynamics in a flowing film and show how a microorganism's ability to resist 'flow-induced-erosion' from interfaces is affected by its elongation and mode of propulsion. Our study generalizes past efforts on understanding microorganism dynamics under confinement by interfaces and provides key insights on biofilm initiation at liquid-liquid interfaces.
The distribution of microorganisms within a film floating over an underlying liquid depends on their morphology and the viscosity ratio across the liquid-liquid interface.
In this paper, we investigate the swimming characteristics and dynamics of a model micro-swimmer in the vicinity of a clean drop, and of a surfactant covered drop. We model the swimmer as a force ...dipole and utilize the image-singularity system to study the dynamical behavior of the swimmer. Motivated by bacterial bio-remediation of insoluble hydrocarbons (HCs) released during oil spills, we report the 'trapping characteristics' - critical trapping radius, basin of attraction and trapping time distribution - of deterministic and stochastic swimmers, as a function of viscosity ratio, and dimensionless surface viscosity. We find that addition of surfactant reduces the critical trapping radius of a drop by ∼30%. The basin of attraction though, is not affected acutely for any combination in the parameter space of viscosity ratio and surface viscosity. We also carry out a dynamical system analysis of our problem, for deterministic swimmers, to clarify the aforementioned concepts. For hydrodynamics combined with diffusion based motion, we note increments ranging from ∼5-25% in the interface-retention times of surfactant-laden drops, as compared to clean drops. These differences occur for low values of surface viscosity, and saturate rapidly as the surface viscosity increases. With potential applications in bioremediation, our results highlight the importance of considering dispersant-addition in oil spills involving insoluble hydrocarbons.
Trajectory of micro-swimmers near a drop; the critical trapping radius of a surfactant-laden drop is smaller than that of a clean drop.
In this study, we document hydrodynamics-mediated trapping of microorganisms around a moving spherical nutrient source such as a settling marine snow aggregate. There exists a range of size and ...excess density of the nutrient source, and motility and morphology of the microorganism under which hydrodynamic interactions enable the passive capture of approaching microorganisms onto a moving nutrient source. We simulate trajectories of chemotactic and non-chemotactic bacteria encountering a sinking marine snow particle effusing soluble nutrients. We calculate the average nutrient concentration to which the bacteria are exposed, under regimes of strong and weak hydrodynamic trapping. We find that hydrodynamic trapping can significantly amplify (by ≈40%) the nutrient exposure of bacteria, both chemotactic and non-chemotactic. The subtle interactions between hydrodynamic and chemotactic effects reveal non-trivial variations in this "hydrodynamic amplification," as a function of relevant biophysical parameters. Our study provides a consistent description of how microorganism motility, fluid flow and nutrient distribution affect foraging by marine microbes, and the formation of biofilms on spherical nutrient sources under the influence of fluid flow.
Purpose Mandating headgear for field players in girls’ lacrosse to reduce head injuries, including concussion, has been heavily debated. However, research regarding the need and effectiveness of ...mandated headgear use in girls’ lacrosse is still developing. Therefore, this qualitative study aimed to identify the need for and barriers to the development of mandated headgear use policies in girls’ lacrosse in Ohio. Methods We conducted six virtual focus groups, three with concussion experts (clinicians and researchers) and three with girls’ lacrosse stakeholders (high school players, parents, coaches, and officials). A focus group guide was developed to explore study participants’ perceptions and opinions on concussion in girls’ lacrosse, headgear use among players, and policies and policy development related to headgear use or a headgear mandate. We developed the codebook using an inductive and iterative approach based on focus group transcripts and used ATLAS.TI to code and analyze the transcript data. Results Concussion experts and stakeholders understood the potential consequences of concussion but did not perceive concussion as a pervasive problem in girls’ lacrosse. The prevention of head and facial injuries was regarded as a potential benefit of headgear use. However, stakeholders expressed that the myriad of arguments discussed opposing mandated headgear use including increased aggressive play and/or targeting, concerns over changes in the game, and cost strongly outweighed the benefits. Finally, both concussion experts and stakeholders identified multiple organizations, including USA Lacrosse, who could act as facilitators and/or barriers to developing, enacting, and implementing headgear policies. Conclusions Concussion experts and stakeholders identified possible reasons for headgear use related to injury prevention but also identified several important barriers to the development of a headgear mandate for girls’ lacrosse in Ohio.
Malignant ameloblastoma is a rare tumor of odontogenic origin with a metastatic focus. Distant metastatic disease is found most commonly in the lungs. A review of the literature shows that most cases ...of malignant ameloblastoma involve a disease-free period from primary tumor extirpation to the discovery of metastasis. This report describes the case of a 56-year-old man presenting with ameloblastoma of the maxilla and a solitary pulmonary metastasis concurrently. This represents a rare case in which there is a simultaneous diagnosis of primary ameloblastoma and a metastatic lesion. Appropriate workup for ameloblastoma includes surveillance for metastatic disease. Surgical resection of primary and distant disease is recommended. Chemotherapy and radiation may play a role in palliation when resection of metastatic disease is not feasible.
In this article, we review mathematical models used to study the behaviour of suspensions of micro-swimmers and the accompanying biophysical phenomena, with specific focus on stimulus response. The ...methods discussed encompass a range of interactions exhibited by the micro-swimmers; including passive hydrodynamic (gyrotaxis) and gravitational (gravitaxis) effects, and active responses to chemical cues (chemotaxis) and light intensities (phototaxis). We introduce the simplest models first, and then build towards more sophisticated recent developments, in the process, identifying the limitations of the former and the new results obtained by the latter. We comment on the accuracy/validity of the models adopted, based on the agreement between theoretical results and experimental observations. We conclude by identifying some of the open problems and associated challenges faced by researchers in the realm of active suspensions.
In this article, we review mathematical models used to study the behaviour of suspensions of micro-swimmers and the accompanying biophysical phenomena, with specific focus on stimulus response.
PDF
