In order to understand the effect of mechanical stretch on corneal extracellular matrix remodeling, human keratoconus fibroblasts (HKCFBs) were subjected to cyclic stretch in vitro and the expression ...of matrix metalloproteinases (MMPs), tissue inhibitor of metalloproteinases (TIMPs), and inflammatory cytokines were evaluated. HKCFBs were seeded into a flexible membrane base and subjected to a cyclic stretch regimen of 10% equibiaxial stretch at a stretching frequency of 1 Hz for 6 h using a Flexcell tension unit. An antibody directed against interleukin‑6 (IL‑6 Ab) was used to investigate the roles of IL‑6 on mechanical stretch mediated regulation of MMP in HKCFBs. Culture supernatants were assayed using an enzyme‑linked immunosorbent assay for MMP‑1 and ‑3, TIMP‑1 and ‑2, and IL‑6. Total RNA from the cells was extracted, and quantitative polymerase chain reaction was used to determine mRNA for MMP‑1 and ‑3, TIMP‑1 and ‑2, and IL‑6. In stretched cells, levels of MMP‑1 and ‑3 demonstrated an increase compared with unstretched cells, but levels of TIMP‑1, and ‑2 revealed a decrease. Mechanical stretch significantly increased the mRNA expression and protein synthesis of IL‑6 compared with unstretched cells. IL‑6 induced MMP‑1 and ‑3 expression, whereas no significant effects were observed in levels of TIMP‑1 and ‑2 compared with the untreated control groups. Additionally, the IL‑6 Ab markedly inhibited the stretch‑induced increase in MMP‑1 and ‑3 in culture supernatants in a dose‑dependent manner. No significant differences in TIMP‑1 and ‑2 protein levels were detected between stretched cells treated with IL‑6 Ab and stretched cells without IL‑6 Ab treatment. These results indicate that cyclical mechanical stretch augments IL‑6 production and MMP expression, and reduces levels of TIMP in HKCFBs. Thus, it is suggested that IL‑6 mediates the stretch‑induced MMP expression.
•Oxygen-compressed bioreactor improved GA production by free and immobilized cells.•Immobilized cells by PVA have shear and autolysis resistance to prolong cell life.•Oxygen-compressed bioreactor ...could reduce oxygen consumption by 30 times.
Bioprocess for Glycolic acid (GA) production from ethylene glycol by whole-cell catalysis of Gluconobacter oxydans is restrained by various biological impediments and high production costs. In this study, these limitations were subsided through the implementation of immobilized whole-cell bio-catalysis combined with increased oxygen supply. Results indicated that this strategy noticeably enhanced mass transfer efficiency, and prolonged cell life that significantly reduced the cost of biomass. Ultimately, with immobilized whole-cell catalysis in air-open and oxygen-open bioreactor, 41.3 and 66.9 g/L of GA was obtained within 48 h, with an increment of 62.0%. Additionally, in oxygen-compressed bioreactor, 63.3 g/L of GA was accumulated with the yield of 97.2%. Subsequently, 605.7 g of GA was produced after 10 rounds of recovery experiments. Although there was a slight decrease in GA production compared with pure-oxygen supply, production cost was reduced with limited oxygen supply. This strategy commendably demonstrated cost-practical bioprocess for GA production.
Butyric acid is a platform chemical material, the production of which has been greatly stimulated by the diverse range of downstream applications in many industries. In particular, higher quality ...butyric acid used in food and medicine, is more dependent on microbiological production methods. Hence, the bio-oxidation of butanol to butyric acid has been identified as a promising method with good potential economic and environmental benefits. However, both butanol and butyric acid are usually intensively toxic to most microorganisms as well as the bio-oxidation pathway. To develop a green, efficient and competitive microbiological method is the primary work to overcome the bottleneck of butyric acid industry.
A combined bioprocess was designed with alternative whole-cell catalysis for butyric acid bio-conversion from butanol by
in a sealed-oxygen supply bioreactor (SOS). In the operation system, the escape of volatile substrates and toxic chemicals to cells can be avoided by the use of a sealed bioreactor, combined with the rejuvenation of cells by supplying energy co-factors. Finally, during a one-batch whole-cell catalysis, the utilization rate of substrate increased from 56.6 to 96.0% by the simple skill. Additionally, the techno-practical bioprocess can realize the purpose of cell-recycling technology through the rejuvenation effect of co-factor. Finally, we obtained 135.3 g/L butyric acid and 216.7 g/L sorbose during a 60-h whole-cell catalysis. This techno-practical technology provides a promising approach to promote the industrial production of butyric acid with more competitiveness.
The techno-practical biotechnology has powerfully promoted the process of butyric acid production by microorganisms, especially makes up for the lack of aerobic fermentation in the industry, and surmounts the shortcomings of traditional anaerobic fermentation. At the same time, this technically practical system provides a promising approach for the promotion of the industrial production of butyric acid in a more competitive manner.
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•Microorganisms were tested in combined electrochemical system for removing EE2.•The degradation rate and growth of the bacteria were influenced by pH and DO levels.•The electric ...current affected bacterial growth and damage by detected MDA and HO.•Shewanella and Aeromonas survival well in the combined system, improving EE2 removal.
17α-ethynylestradiol (EE2) has become a growing concern due to its wide detection and high estrogenic potency. However, biodegradation of EE2 with individual microorganisms demonstrated poor degradation efficiency ranging from 10.3% to 45.8% in 120h. Now, a system combining electrochemical with biological degradation was tested for its ability to remove EE2 from aqueous solution. EE2 removal is promoted at high dissolved oxygen concentrations and low pH when electrochemical methods alone are employed. Coupling electrochemical methods with anaerobic bacteria can improve the EE2 removal after 90min to as much as 81.9% with a reaction rate constant of 0.0195min−1. An electric current of 100mA at pH5.5 inhibits the growth of most microbial species, but Shewanella and Aeromonas survive well. They can enhance the efficiency of direct electron transfer efficiency between the bacteria and the electrodes, promoting EE2 degradation. This study provided a theoretical basis for the future applicability of bioelectrochemical technology to remove steroids from waste water.
The sandwich composite wall with truss connectors is a new type of double-steel-plate composite wall, which consists of two external steel plates, square steel tubes at both ends, and in-fill ...concrete. The external steel plates are constrained by the steel trusses, thereby possessing superior mechanical performance. In order to study the fire resistance of the sandwich composite wall, 4 full-scaled walls were tested under one-side ISO-834 standard fire, aiming to investigate the effects of axial compression ratio, truss spacing to thickness ratio, and steel plate thickness on the fire resistance of the walls. The temperature, axial displacement and transverse displacement of key parts of the walls were measured. Experimental results indicate that, the walls could maintain sufficient load-bearing capacity as specified by the Class I fire resistance rating of Chinese Code. The fire endurance of the wall was controlled by its thermal insulation. The deformation pattern of the walls under the fire was overall deflection toward the unexposed surface, and the steel plate exposed to the fire experienced obvious local buckling. The axial compression ratio and the truss spacing to thickness ratio had great effects on the deformation behavior of the walls. When the axial compression ratio was reduced from 0.5 to 0.3, the axial deformation of the wall was changed from compression-predominant to expansion-predominant deformation. A larger truss spacing to thickness ratio led to more obvious buckling of the steel plate exposed to the fire. It is recommended to restrict the truss spacing to thickness ratio of the wall in engineering design.
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•Full-scaled walls were tested under one-side fire.•The temperatureand the displacement of the walls were measured and discussed.•Deformation pattern of the walls was overall deflection toward the unexposed surface.•The fire endurance of the wall was controlled by its thermal insulation.•The axial compression ratio and the truss spacing to thickness ratiowere main parameters.
Veno-venous extracorporeal membrane oxygenation (ECMO) was successfully performed for the rescue of an adult patient with severe acute respiratory distress syndrome (ARDS) induced by fulminant ...psittacosis, and then a near-fatal pulmonary embolism (PE) and cardiac arrest (CA) of the same patient was cured through catheter-directed thrombolysis.
A 51-year-old female patient was admitted to the hospital on September 10, 2021 due to slurred speech, weakness in lower limbs, dizziness, and nausea. Subsequently, she developed confusion and was transferred to the intensive care unit (ICU), where she received anti-shock, antibiotics, invasive mechanical ventilation (IMV), and veno-venous ECMO due to the diagnosis of severe pneumonia, severe ARDS, and septic shock based on comprehensive physical examination, laboratory tests, and imaging findings. The metagenomic next-gengeration sequencing (m-NGS) in the bronchoalveolar lavage fluid (BALF) suggested that the pathogen was chlamydia psittaci, so the antibiotics were adjusted to doxycycline combined with azithromycin. After withdrawal from ECMO, ultrasound (US) re-examination of the left lower limb revealed inter-muscular vein thrombosis, following which heparin was replaced by subcutaneous injection of 0.4ml enoxaparin sodium twice daily for anti-coagulation therapy. After withdrawal from IMV, the patient suffered sudden CA and successful cardiopulmonary resuscitation (CPR), and emergency pulmonary angiography (PA) was performed to show bilateral main pulmonary artery embolism. After immediate catheter-directed thrombolysis and placement of an inferior vena cava filter, the patient's condition gradually stabilized.
Veno-venous ECMO can be successfully performed as an emergency life-saving treatment for patients with severe ARDS induced by fulminant psittacosis, and during ECMO regular examinations should be conducted to detect and manage thrombosis in time, thereby avoiding the occurrence of near-fatal PE and CA.
Little is known on the cost-effectiveness of novel regimens for hepatitis C virus (HCV) compared with standard-of-care with pegylated interferon (pegIFN) and ribavirin (RBV) therapy in developing ...countries. We evaluated cost-effectiveness of sofosbuvir/ledipasvir for 12 weeks compared with a 48-week pegIFN-RBV regimen in Chinese patients with genotype 1b HCV infection by economic regions.
A decision analytic Markov model was developed to estimate quality-adjusted-life-years, lifetime cost of HCV infection and incremental cost-effectiveness ratios (ICERs). SVR rates and direct medical costs were obtained from real-world data. Parameter uncertainty was assessed by one-way and probabilistic sensitivity analyses. Threshold analysis was conducted to estimate the price which can make the regimen cost-effective and affordable.
Sofosbuvir/ledipasvir was cost-effective in treatment-experienced patients with an ICER of US$21,612. It varied by economic regions. The probability of cost-effectiveness was 18% and 47% for treatment-naive and experienced patients, and it ranged from 15% in treatment-naïve patients in Central-China to 64% in treatment-experienced patients in Eastern-China. The price of 12-week sofosbuvir/ledipasvir treatment needs to be reduced by at least 81% to US$18,185 to make the regimen cost-effective in all patients at WTP of one time GDP per capita. The price has to be US$105 to make the regimen affordable in average patients in China.
Sofosbuvir/ledipasvir regimen is not cost-effective in most Chinese patients with genotype 1b HCV infection. The results vary by economic regions. Drug price of sofosbuvir/ledipasvir needs to be substantially reduced when entering the market in China to ensure the widest accessibility.
BackgroundVeno-venous extracorporeal membrane oxygenation (ECMO) was successfully performed for the rescue of an adult patient with severe acute respiratory distress syndrome (ARDS) induced by ...fulminant psittacosis, and then a near-fatal pulmonary embolism (PE) and cardiac arrest (CA) of the same patient was cured through catheter-directed thrombolysis. Case presentationA 51-year-old female patient was admitted to the hospital on September 10, 2021 due to slurred speech, weakness in lower limbs, dizziness, and nausea. Subsequently, she developed confusion and was transferred to the intensive care unit (ICU), where she received anti-shock, antibiotics, invasive mechanical ventilation (IMV), and veno-venous ECMO due to the diagnosis of severe pneumonia, severe ARDS, and septic shock based on comprehensive physical examination, laboratory tests, and imaging findings. The metagenomic next-gengeration sequencing (m-NGS) in the bronchoalveolar lavage fluid (BALF) suggested that the pathogen was chlamydia psittaci, so the antibiotics were adjusted to doxycycline combined with azithromycin. After withdrawal from ECMO, ultrasound (US) re-examination of the left lower limb revealed inter-muscular vein thrombosis, following which heparin was replaced by subcutaneous injection of 0.4ml enoxaparin sodium twice daily for anti-coagulation therapy. After withdrawal from IMV, the patient suffered sudden CA and successful cardiopulmonary resuscitation (CPR), and emergency pulmonary angiography (PA) was performed to show bilateral main pulmonary artery embolism. After immediate catheter-directed thrombolysis and placement of an inferior vena cava filter, the patient's condition gradually stabilized. ConclusionsVeno-venous ECMO can be successfully performed as an emergency life-saving treatment for patients with severe ARDS induced by fulminant psittacosis, and during ECMO regular examinations should be conducted to detect and manage thrombosis in time, thereby avoiding the occurrence of near-fatal PE and CA.
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