Background
Sepsis reaction is a response to an infection composed of genetic elements. This research aims to better understand how sepsis affects the molecular pathways in whole blood samples.
...Methods
Whole blood samples from healthy controls (
n
= 18), sepsis nonsurvivors (
n
= 9), and sepsis survivors (
n
= 26) were retrieved from the gene expression omnibus (GEO) collection of the national center for biotechnology information (NCBI) (accession number GSE54514). The NCBI's GEO2R program was used to determine differential expression, and the ingenuity pathway analysis (IPA) software was utilized to do a pathway analysis.
Results
In sepsis patients, 2672 genes were substantially differently expressed (
p
value 0.05). One thousand three hundred four genes were overexpressed, and one thousand three hundred sixty-eight were under-expressed. The inhibition of ARE-mediated mRNA degradation pathway and the Pl3K/AKT signaling spliceosomal cycle were the most significant canonical pathways identified by ingenuity pathway analysis (IPA). The IPA upstream analysis predicted the ESR1, SIRT1, and PTPRR proteins, and the drugs filgrastim and fluticasone were top transcriptional regulators.
Conclusions
The inhibition of ARE-mediated mRNA degradation pathway and the Pl3K/AKT signaling spliceosomal cycle were highlighted as essential pathways of inflammation by IPA, indicating widespread cancer owing to sepsis. Our data imply that sepsis considerably influences gene pathways in whole blood samples, pointing to possible targets for sepsis treatment.
Background: On March 2020, World Health Organization (WHO) labeled coronavirus disease 2019 (COVID-19) as a pandemic. COVID-19 has rapidly increased in Jordan which resulted in the announcement of ...the emergency state on March 19th, 2020. Despite the variety of research being reported, there is no agreement on the variables that predict COVID-19 infection. We have analyzed the data collected from Karak city citizens to predict the probability of infection with COVID-19 using binary logistic regression model.
Methods: Based on data collected by Google sheet of COVID-19 infected and non-infected persons in Karak city, analysis was applied to predict COVID-19 infection probability using a binary logistic regression model.
Results: The ultimate logistic regression model provides the formula of COVID-19 infection probability based on sex and age variables.
Conclusions: Given a person's age and sex, the final model presented in this study can be used to calculate the probability of infection with COVID-19 in Karak city. This could help aid health-care management and policymakers in properly planning and allocating health-care resources.
Background:
On March 2020, World Health Organization (WHO) labeled coronavirus disease 2019 (COVID-19) as a pandemic. COVID-19 has rapidly increased in Jordan which resulted in the announcement of ...the emergency state on March 19th, 2020. Despite the variety of research being reported, there is no agreement on the variables that predict COVID-19 infection. We have analyzed the data collected from Karak city citizens to predict the probability of infection with COVID-19 using binary logistic regression model.
Methods: Based on data collected by Google sheet of COVID-19 infected and non-infected persons in Karak city, analysis was applied to predict COVID-19 infection probability using a binary logistic regression model.
Results:
The ultimate logistic regression model provides the formula of COVID-19 infection probability based on sex and age variables.
Conclusions: Given a person's age and sex, the final model presented in this study can be used to calculate the probability of infection with COVID-19 in Karak city. This could help aid health-care management and policymakers in properly planning and allocating health-care resources.
Background: Colorectal cancer (CRC) is currently the third most common cancer type in males and the second most occurring in females. The role of microRNA (miRNA) in the development of colorectal ...cancer is not fully elucidated. Therefore, understanding the mechanistic interaction between miRNA and their target oncogenes may hold great importance as a possible target for interventional anticancer therapy.
Aims: To identify miRNAs that are part of the regulating pathway of Monocarboxylate Transporter-4 (MCT4) and Vascular Endothelial Growth Factor (VEGF) oncogenes.
Study Design: We used publicly available prediction tools (e.g. TargetScan, MicroCosm, PicTar, and DIANA-microT-CDS) to identify the possible miRNA that target the two oncogenes.
Methodology: We used the GeneMania database to visualize the network and verify gene names and remove ambiguity and duplications. Furthermore, we used miRTarBase database to identify experimentally validated targets which we used to further confirm miRNA-oncogene relationships. Finally, we utilized miR-Mfold web-tool to further visualize the circular structures and the simulated miR-1 and miR-206 targeting arrangements.
Results: We found two putative miRNA (miR-1 and miR-206) that may downregulate MCT4 coded by SLC16A3 gene and VEGF which is coded by VEGF gene. We found relationships between the validated target genes of miR-1 and miR-206 through GeneMania which we extracted from the literature. And we elucidated the proposed structure of these two miRNAs through miR-Mfold web-tool.
Conclusion: Our results elucidated a novel regulation pathway in CRC cells and may suggest a potential therapeutic approach for CRC therapy. MiR-1 and miR-206 may help cells go to apoptosis and inhibit the angiogenesis of colorectal cancer cells by down-regulation of MCT4 and VEGF proteins in tumor tissues.
Breast cancer screening has been proven effective in Western countries, and our National Health Bureau also started to offer free screening mammography for women aged between 50 and 69, since July ...2004. This study aims to compare the efficacy between distinctive mammography screening modalities. Prompting the assessment of digital screening in the radiological sciences, we provide insight into the practical informatics application of such tools.
碩士
國立陽明大學
生物醫學影像暨放射科學系
102
Background and Objective: Chronic liver disease and cirrhosis result in about 35,000 deaths each year in the United States. Cirrhosis is the ninth leading cause of death in ...the United States and is responsible for 1.2% of all US deaths. MRI has been reported to be an excellent imaging modality because of its high tissue contrast and use of tissue-specific contrast agents. Gadoxetic acid (Gd-EOB-DTPA) is a more recently developed liver specific MR imaging contrast agent. The excretion of Gd-EOB-DTPA might be related to the liver disease and liver function. The pur¬pose of this study was to determine transit times for excretion of Gd-EOB-DTPA in the hepatobiliary system in patients with liver cirrhosis1.
Material and Methods: Institutional Review Board of our hospital approved the study; in-formed consent was waived. We retrospectively included consecutive patients with liver cirrho¬sis who underwent contrast-enhanced abdominal MR examination after injection of 10 mL Gd-EOB-DTPA at 1.5-T MR from December 2009 to March 2012. Finally, the 47 patients were en-rolled in this study.
The Gd-EOB-DTPA-enhanced MRI included arterial phase (18~20sec, arterial phase, AP), por¬tal venous phase (50~55 sec, portal venous phase, PP), venous phase (85~90s, venous phase, VP) and three delayed phases (180s, 20mins and 30mins after the injection of contrast agent). The images were evaluated for the presence of contrast agent in the intra-hepatic bile ducts (IHD), the common bile duct (CBD), the gallbladder and the duodenum in AP, PP, VP and three delay phases.
Results: The optimal time of arterial phase was from 15 s after injection, and the optimal time for portal venous imaging was from 40 s after injection. Meanwhile, the optimal time to observe changes was 20 min after contrast initiation of Gd-EOB-DTPA in 39 patients (39/47, 83 %) at the IHD and 37 patients (37/47, 78.5%) at the CBD. Gallbladder reflux was visible in 26 (26/47, 43%), duodenal excretion in 17 patients (17/47, 36%), respectively. After 30 min of contrast injection, Gd-EOB-DTPA could still be detected in 6 patients (6/47, 13 %) at the IHD and 7 patients (7/47, 15 %) at the CBD, and gallbladder reflux was visible in 10 (10/47, 21%), duodenal excretion in 20 patients (26/47, 55%), respectively.(Fig 2 and Fig 3) GB was not
visible in 9 patients, 4 out of these 9 patients received cholecystectomy.
Conclusion: The results of our study shows that in patients with liver cirrhosis, the excretion of Gd-EOB-DTPA can be observed. Our results also demonstrated that the bilirubin level and the severity of cirrhosis (according to the Child Pugh scores had certain effect on excretion of Gd-EOB-DTPA into biliary tree.
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