Madagascar, one of the top megadiversity regions, hosts one of the highest numbers of endemic and threatened organisms on earth. One of the most spectacular examples of ant radiation on the island ...has occurred in the hyperdiverse genus Pheidole. To this date, there are 117 described Madagascan Pheidole divided into 16 species-groups, and 97% of them are endemic to the island. Only two of these species-groups contain widely distributed invasive species in addition to native, endemic taxa: megacephala, and fervens species-groups. Here we revise the fervens species-group and discuss updated distribution records of its introduced members on Madagascar. We recognize six species belonging to this group, including five new to science: Pheidole ampangabe sp. nov., P. arivo sp. nov., P. comosa sp. nov., P. indica Mayr, P. mamirapiratra sp. nov., and P. mena sp. nov. Detailed descriptions are supplemented with measurements, diagnoses, identification key, high-resolution images for major and minor worker, and comments on distribution and biology.
In a male-based revision of ants of the subfamily Amblyoponinae from the Southwest Indian Ocean islands (SWIO: Comoros, Madagascar, Mauritius, Mayotte, Reunion, and Seychelles), we explore and ...reconsider male morphological characters that distinguish genera within the group. Our investigation redefines Amblyopone Erichson sensu Brown (1960), here referred to as Amblyopone sensu lato, into three genera: Xymmer Santschi stat. rev.,Amblyopone sensu stricto, Stigmatomma Roger stat. rev. All species names under Amblyopone s. l. reassign into Xymmer and Amblyopone s. s., which are small, well-defined genera, and Stigmatomma, a large group with a generic delimitation that still needs further refinement. Based on a study of male mandible characters and our scenario for mandibular evolution of the worker caste within Amblyopone s. l, we conclude that Amblyopone s. s. nests outside of XMAS (Xymmer+Mystrium+Adetomyrma+Stigmatomma) clade. The following names are transferred from Amblyopone s. l. to Xymmer as comb. rev.: muticus. The following names are transferred from Amblyopone s. l. to Stigmatomma as comb. rev.: amblyops, armigerum, bellii, bierigi, bruni, celata, chilense, denticulatum, elongatum, emeryi, feae, impressifrons, luzonicum, minuta, normandi, oregonense, pallipes, quadratum, reclinatum, rothneyi, santschii, saundersi, silvestrii, zwaluwenburgi; as comb. nov.: agostii, annae, besucheti, boltoni, caliginosum, cleae, crenatum, degeneratum, egregium, electrinum, eminia, exiguum, falcatum, ferrugineum, fulvidum, gaetulicum, gingivalis, glauerti, gnoma, gracile, groehni, heraldoi, lucidum, lurilabes, monrosi, mystriops, noonadan, octodentatum, ophthalmicum, orizabanum, papuanum, pertinax, pluto, punctulatum, rubiginoum, sakaii, smithi, trigonignathum, trilobum, wilsoni, zaojun, and testaceum. A male-based key to the genera of Malagasy amblyoponine ants, their diagnoses, and a discussion of the evolution of the morphological character of males in the subfamily are given, and the distinguishing characters of each are illustrated. In addition, our results predict that Paraprionopelta belongs in the XMAS clade and that Concoctio should have males with two mandibular teeth.
Termites host diverse communities of gut microbes, including many bacterial lineages only found in this habitat. The bacteria endemic to termite guts are transmitted via two routes: a vertical route ...from parent colonies to daughter colonies and a horizontal route between colonies sometimes belonging to different termite species. The relative importance of both transmission routes in shaping the gut microbiota of termites remains unknown. Using bacterial marker genes derived from the gut metagenomes of 197 termites and one
cockroach, we show that bacteria endemic to termite guts are mostly transferred vertically. We identified 18 lineages of gut bacteria showing cophylogenetic patterns with termites over tens of millions of years. Horizontal transfer rates estimated for 16 bacterial lineages were within the range of those estimated for 15 mitochondrial genes, suggesting that horizontal transfers are uncommon and vertical transfers are the dominant transmission route in these lineages. Some of these associations probably date back more than 150 million years and are an order of magnitude older than the cophylogenetic patterns between mammalian hosts and their gut bacteria. Our results suggest that termites have cospeciated with their gut bacteria since first appearing in the geological record.
The evolution of eusociality has allowed ants to become one of the most conspicuous and ecologically dominant groups of organisms in the world. A large majority of the current ∼14,000 ant species ...belong to the formicoids,1 a clade of nine subfamilies that exhibit the most extreme forms of reproductive division of labor, large colony size,2 worker polymorphism,3 and extended queen longevity.4 The eight remaining non-formicoid subfamilies are less well studied, with few genomes having been sequenced so far and unclear phylogenetic relationships.5 By sequencing 65 genomes, we provide a robust phylogeny of the 17 ant subfamilies, retrieving high support to the controversial leptanillomorph clade (Leptanillinae and Martialinae) as the sister group to all other extant ants. Moreover, our genomic analyses revealed that the emergence of the formicoids was accompanied by an elevated number of positive selection events. Importantly, the top three gene functions under selection are linked to key features of complex eusociality, with histone acetylation being implicated in caste differentiation, gene silencing by RNA in worker sterility, and autophagy in longevity. These results show that the key pathways associated with eusociality have been under strong selection during the Cretaceous, suggesting that the molecular foundations of complex eusociality may have evolved rapidly in less than 20 Ma.
•We sequenced 65 ant genomes representing the 17 ant subfamilies•Leptanillomorphs are the sister clade of all other extant ant species•A natural selection hotspot occurred during the emergence of formicoids•Genomic foundations of complex eusociality may have evolved early in ant evolution
Romiguier et al. sequence 65 genomes to produce a phylogenetic tree of all ant subfamilies, with the subterranean leptanillomorph clade as the sister group of all other ants. A natural selection hotspot is detected during the emergence of formicoid clade, which is an ant clade grouping the most complex forms of sociality.
Background
To the authors' knowledge, studies regarding risk perception among survivors of thyroid cancer are scarce.
Methods
The authors surveyed patients who were diagnosed with differentiated ...thyroid cancer from the Surveillance, Epidemiology, and End Results registries of Georgia and Los Angeles County (2632 patients; 63% response rate). The analytic cohort was defined by a ≤5% risk of disease recurrence and mortality (1597 patients). Patients estimated their recurrence and mortality risks separately (increments of 10% and endpoints of ≤5% and ≥95%). Both outcomes were dichotomized between reasonably accurate estimates (risk perception of ≤5% or 10%) versus overestimation (risk perception of ≥20%). Multivariable logistic regression was used to identify factors associated with risk overestimation, and the relationships between overestimation and both worry and quality of life were evaluated.
Results
In the current study sample, 24.7% of patients overestimated their recurrence risk and 12.5% overestimated their mortality risk. A lower educational level was associated with overestimating disease recurrence (≤high school diploma: odds ratio OR, 1.64 95% CI, 1.16‐2.31; and some college: OR, 1.36 95% CI, 1.02‐1.81) and mortality (≤high school diploma: OR, 1.86 95% CI, 1.18‐2.93) risk compared with those attaining at least a college degree. Hispanic ethnicity was found to be associated with overestimating recurrence risk (OR, 1.44, 95% CI 1.02‐2.03) compared with their white counterparts. Worry about recurrence and death was found to be greater among patients who overestimated versus those who had a reasonably accurate estimate of their risk of disease recurrence and mortality, respectively (P < .001). Patients who overestimated mortality risk also reported a decreased physical quality of life (mean T score, 43.1; 95% CI, 41.6‐44.7) compared with the general population.
Conclusions
Less educated patients and Hispanic patients were more likely to report inaccurate risk perceptions, which were associated with worry and a decreased quality of life.
Less educated and Hispanic survivors of thyroid cancer appear to be more likely to overestimate their recurrence risk, with the former also more likely to overestimate their mortality risk. These inaccurate risk perceptions are associated with increased worry regarding disease recurrence and death, respectively, and decreased physical quality of life among those who overestimate their mortality risk.
Children, adolescents, and young adults receiving intensive chemotherapy for acute myeloid leukemia or high-risk or relapsed acute lymphoblastic leukemia sustain prolonged periods of neutropenia that ...predispose them to invasive fungal disease (IFD). For many decades the standard of care for these patients was to initiate empirical antifungal therapy after a period of prolonged fever and neutropenia. Recent publications have yielded important evidence on the utility of different diagnostic and therapeutic approaches aimed at reducing the impact of IFD among these patients during these vulnerable periods. This case-based review highlights and interprets the published data to provide context for the IFD diagnostic and therapeutic recommendations proposed in multiple published guidelines. Personalized approaches are offered at points where evidence is lacking. Time points where specific knowledge gaps exist are identified along the clinical trajectory of the prolonged neutropenic period to illustrate areas for future investigation.
With increasing frequency, patients are being asked to make complex decisions about cancer screening, prevention, and treatment. These decisions are fraught with emotion and cognitive difficulty ...simultaneously. Many Americans have low numeracy skills making the cognitive demands even greater whenever, as is often the case, patients are presented with risk statistics and asked to make comparisons between the risks and benefits of multiple options and to make informed medical decisions. In this commentary, we highlight 10 methods that have been empirically shown to improve patients' understanding of risk and benefit information and/or their decision making. The methods range from presenting absolute risks using frequencies (rather than presenting relative risks) to using a risk format that clarifies how treatment changes risks from preexisting baseline levels to using plain language. We then provide recommendations for how health-care providers and health educators can best to communicate this complex medical information to patients, including using plain language, pictographs, and absolute risks instead of relative risks.
Depression is often framed as a disease or dysfunctional syndrome, yet this framing has unintended negative consequences including increased stigma. Here, we consider an alternative messaging ...framework – that depression serves an adaptive function. We describe the historical development of popular messages about depression and draw from the fields of evolutionary psychiatry and social cognition to describe the alternative framework that depression is a “signal” that serves a purpose. We then present data from a pre-registered, online randomized-controlled study in which participants with self-reported depression histories viewed a series of videos that explained depression as a “disease like any other” with known biopsychosocial risk factors (BPS condition), or as a signal that serves an adaptive function (Signal condition). In the entire sample (N = 877), three of the six hypotheses were supported: The Signal condition led to less self-stigma, greater offset efficacy, and more adaptive beliefs about depression. Exploratory analyses revealed these Signal effects were stronger among females (N = 553), who also showed a greater growth mindset of depression after the Signal explanation. Results suggest that framing depression as an adaptive signal can benefit patients and avoid harmful consequences of popular etiological presentations. We conclude that alternative framings of depression are worthy of further study.
•Depression is often discussed as a medical disorder.•This framework has some benefits but also has some drawbacks.•We compared the disease framework to a novel framing of depression as a signal.•Framing depression as a signal led to less stigma and more hope about recovery.•Framing depression as a signal had no impact on treatment attitudes.
Making evidence-based decisions often requires comparison of two or more options. Research-based evidence may exist which quantifies how likely the outcomes are for each option. Understanding these ...numeric estimates improves patients' risk perception and leads to better informed decision making. This paper summarises current "best practices" in communication of evidence-based numeric outcomes for developers of patient decision aids (PtDAs) and other health communication tools.
An expert consensus group of fourteen researchers from North America, Europe, and Australasia identified eleven main issues in risk communication. Two experts for each issue wrote a "state of the art" summary of best evidence, drawing on the PtDA, health, psychological, and broader scientific literature. In addition, commonly used terms were defined and a set of guiding principles and key messages derived from the results.
The eleven key components of risk communication were: 1) Presenting the chance an event will occur; 2) Presenting changes in numeric outcomes; 3) Outcome estimates for test and screening decisions; 4) Numeric estimates in context and with evaluative labels; 5) Conveying uncertainty; 6) Visual formats; 7) Tailoring estimates; 8) Formats for understanding outcomes over time; 9) Narrative methods for conveying the chance of an event; 10) Important skills for understanding numerical estimates; and 11) Interactive web-based formats. Guiding principles from the evidence summaries advise that risk communication formats should reflect the task required of the user, should always define a relevant reference class (i.e., denominator) over time, should aim to use a consistent format throughout documents, should avoid "1 in x" formats and variable denominators, consider the magnitude of numbers used and the possibility of format bias, and should take into account the numeracy and graph literacy of the audience.
A substantial and rapidly expanding evidence base exists for risk communication. Developers of tools to facilitate evidence-based decision making should apply these principles to improve the quality of risk communication in practice.
Abstract
Halocyclization of alkenes is a powerful bond-forming tool in synthetic organic chemistry and a key step in natural product biosynthesis, but catalyzing halocyclization with high ...enantioselectivity remains a challenging task. Identifying suitable enzymes that catalyze enantioselective halocyclization of simple olefins would therefore have significant synthetic value. Flavin-dependent halogenases (FDHs) catalyze halogenation of arene and enol(ate) substrates. Herein, we reveal that FDHs engineered to catalyze site-selective aromatic halogenation also catalyze non-native bromolactonization of olefins with high enantioselectivity and near-native catalytic proficiency. Highly selective halocyclization is achieved by characterizing and mitigating the release of HOBr from the FDH active site using a combination of reaction optimization and protein engineering. The structural origins of improvements imparted by mutations responsible for the emergence of halocyclase activity are discussed. This expansion of FDH catalytic activity presages the development of a wide range of biocatalytic halogenation reactions.
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