In this study, we performed an adipogenic differentiation of human adipose-derived stem cells (ADSCs) in vitro with different deuterium content (natural, low and high) in the culture medium during ...differentiation process with parallel analysis of the gene expression, metabolic activity and cell viability/toxicity. After ADSCs differentiation into adipocytes we have done the analysis of differentiation process efficiency and determined a type of resulting adipocytes (by morphology, gene expression, UCP1 protein detection and adipokine production analysis). We have found that high (5 × 10
ppm) deuterium content significantly inhibit in vitro adipogenic differentiation of human ADSCs compared to the groups with natural (150 ppm) and low (30 ppm) deuterium content. Importantly, protocol of differentiation used in our study leads to white adipocytes development in groups with natural (control) and high deuterium content, whereas deuterium-depleted differentiation medium leads to brown-like (beige) adipocytes formation. We have also remarked the direct impact of deuterium on the cellular survival and metabolic activity. Interesting, in deuterium depleted-medium, the cells had normal survival rate and high metabolic activity, whereas the inhibitory effect of deuterated medium on ADSCs differentiation at least was partly associated with deuterium cytotoxicity and inhibitory effect on metabolic activity. The inhibitory effect of deuterium on metabolic activity and the subsequent decrease in the effectiveness of adipogenic differentiation is probably associated with mitochondrial dysfunction. Thus, deuterium could be considered as an element that affects the substance chirality. These findings may be the basis for the development of new approaches in the treatment of obesity, metabolic syndrome and diabetes through the regulation of adipose-derived stem cell differentiation and adipocyte functions.
The kinetics of biological reactions depends on the deuterium/protium (D/H) ratio in water. In this work, we describe the kinetic model of biocatalytic reactions in living organisms depending on the ...D/H ratio. We show that a change in the lifetime or other characteristics of the vital activity of some organisms in response to a decrease or increase in the content of deuterium in the environment can be a sign of a difference in taxons. For animals-this is a curve with saturation according to the Gauss's principle, for plants-it is the Poisson dependence, for bacteria a weakly saturated curve with a slight reaction to the deuterium/protium ratio toward increasing deuterium. The biological activity of the aquatic environment with reduced, elevated, and natural concentrations of deuterium is considered. The results of the study are presented in different vital indicators of some taxons: the bacteria kingdom-the colony forming units (CFU) index (
); animals-the activation energy of the death of ciliates (
), embryogenesis of fish (
)
plants-germination and accumulation of trace elements
L., sprouting of gametophores and peptidomics of moss
. It was found that many organisms change their metabolism and activity, responding to both high and low concentrations of deuterium in water.
Porcine circovirus type 3 (PCV3) was firstly detected in 2016 in USA. Later PCV3 was discovered in Asia, Europe, and South America. The present investigation demonstrates for the first time the ...circulation of PCV3 among pigs in Russia. The viruses were detected at two geographically distant unrelated commercial farms with records of reproductive failure (abortions, stillbirth), porcine dermatitis, and nephropathy syndrome (PDNS). The two farms were located in the region of Smolensk (western part of Russia) and the region of Tyumen (West Siberia, Russia). We investigated samples collected from pigs of different ages. We performed PCR for the PCV3 DNA detection. The DNA of PCV3 was detected in serum, kidney, heart, spleen, pleural effusion, and peritoneal cavity fluid samples. Two viral genomes were sequenced and the corresponding strains were named PCV3-RU/SM17 (the strain from Smolensk region) and PCV3-RU/TY17 (the strain from Tyumen region). The full genome sequences of both strains were 2000 nucleotides in length and contained at least two ORFs, encoding the Cap and Rep proteins. Full sequence alignment revealed a 99.3% identity between the PCV3-RU/SM17 and PCV3-RU/TY17 strains. Molecular analysis showed that the two strains from Russia are highly homologous to viruses identified in other countries, with a 98.5–99.6% homology for PCV3-RU/TY17 and 97.9–99.0 for PCV3-RU/SM17. The PCV3-RU/SM17 and PCV3-RU/TY17 strains were found to form a monophyletic group in a phylogenetic tree based on PCV3 complete genome sequences.
Two late Holocene cold events were described for the Southern Russian Far East: 2800–2600 year BP and the Little Ice Age (LIA) (~1300–1850 CE). The synthesis is based on multi-proxy records on ...profile “the mainland (Primorye)-islands (Sakhalin-Kurils)”. Main archives are sediments of small lakes and peat bogs that recorded the high-resolution environmental changes. The temporal resolution of reconstructions here is up to 26–40 years. During the cold event of 2800–2600 year BP, the humidity decreased sharply, there were long-term dry seasons without strong floods, and buried soil formed on floodplains. The LIA was wet in the mainland and the Kurils, with frequent strong floods, but was drier in Sakhalin. The cooling was characterized by sharp temperature fluctuations where high moisture conditions alternated with short-term drier periods. The shift in geographical position and intensity of the main centers of atmospheric action caused a paleoclimatic interpretation of these events (Aleutian Low, Siberian and North Pacific Highs, the summer Okhotsk anticyclone and the Far East depression). Changes in the North Pacific oscillations played an important role in the alternation of cold-dry and cold-wet periods. Anomalies in the intensity of El Niño and the monsoon system led to changes in tropical and extratropical cyclone trajectories and cyclogenesis in general.
Previous studies have shown that complexly shaped nanoparticles (NPs) have their intrinsic radiothermal emission in the millimeter range. This article presents a method for controlling the quality of ...nanodrugs-immunobiological preparations (IBPs)-based on the detection of their intrinsic radiothermal emissions. The emissivity of interferon (IFN) medicals, determined without opening the primary package, is as follows (µW/m
): IFN-α2b-80 ± 9 (10
IU per package), IFN-β1a-40 ± 5 (24 × 10
IU per package), IFN-γ-30 ± 4 (10
IU per package). The emissivity of virus-like particles (VLP), determined using vaccines Gam-VLP-multivac (120 μg) in an injection bottle (crimp cap vials), was as follows: 12 ± 1 µW/m
, Gam-VLP-rota vaccines-9 ± 1 µW/m
. This study shows the reproducibility of emissivity over the course of a year, subject to the storage conditions of the immunobiological products. It has been shown that accelerated aging and a longer shelf life are accompanied by the coagulation of active NPs, and lead to a manyfold drop in emissivity. The dependence of radiothermal emission on temperature has a complex, non-monotonic nature. The emission intensity depends on the form of dosage, but remains within the order of magnitude for IFN-α2b for intranasal aqueous solution, ointments, and suppositories. The possibility of the remote quantitative control of the first phases of the immune response (increased synthesis of IFNs) to the intranasal administration of VLP vaccines has been demonstrated in experimental animals.
•New data on climatic changes at the late Pleistocene-Holocene boundary for Kurils.•Climate fluctuations and volcanic ash-falls were mail drivers in lake-mire evolution.•The lake level was highest ...during warmer and humid climate of the Allerød.•Younger Dryas cooling in the Kuril Islands was less distinct than on the continent.•Pollen record vegetation changes on the land bridge at Late Glacial–early Holocene.
Despite considerable progress in understanding Late Pleistocene and Holocene palaeoenvironmental changes worldwide, there are still very few such data for the Far East, and Kuril islands, in particular. Here, the sea level rise during the Late Glacial–Holocene transition was accompanied by a loss of the land connectivity culminating into the development of isolated islands. The ecosystems evolution and importance of isolation for vegetation dynamics on islands are still insufficiently understood. We investigated a unique natural record of environmental change since 14 ka has been recently discovered in lake–mire complex on Kunashir Island. The sediments include 12 tephra layers from Kunashir and Hokkaido volcanoes. The lake level was highest during warmer and humid climate of the Allerød. Diatom assemblages indicated the higher lake level between 12730–12160 cal yr BP. The first half of Younger Dryas was characterized by heavy snowfalls. Cooling attributed to the Younger Dryas in the Southern Kurils was less distinct than on the continent. The pollen assemblages recorded changes in vegetation on the land bridge. At the end of the Pleistocene large areas were occupied by birch forests and shrub pine. The broadleaf species first appeared at the end of Allerød. Starting from the Pleistocene-Holocene transition and during the early Holocene, the role of conifers sharply increased. In the middle Holocene the proportion of broadleaf trees in forests increased. Coniferous forests became abundant during the Little Ice Age. Short-tern climatic fluctuations correlate well with regional pattern, particularly with fluctuations of the moisture supply.
There is little information about moisture changes in different altitudinal belts in mountainous regions of the southern Russian Far East. We present ecological and taxonomic compositions of the ...diatom flora and identify the botanical composition of peat in small mountain lake/mire complexes located in the Central Sikhote-Alin Mountains, within large landslides on the paleovolcanic slopes. Frequent changes in diatom assemblages and peat-forming plants indicate unstable hydroclimatic conditions with varying degrees of wet and dry conditions up to the overgrowth of the lakes. Frequent change in sphagnum mosses with different trophic preferences was identified. The chronology is based on 11 radiocarbon dates. Accumulation rates reached 1.7-1.9 mm/year, and the temporal resolution for the reconstructions was up to 30-40 yr. The tendencies of lake evolution depended on different scale hydroclimatic changes over the last 4400 yr. The most detailed data for the last 2600 yr were obtained from the Nizhnee Lake sequence, which is more sensitive to climatic changes. The main reason for the change in the hydrological regime of the lakes was variations in precipitation during short-term climatic changes. The sediment record of moisture fluctuations is relatively well correlated with regional patterns reflecting summer monsoon intensity and cyclogenesis activity.
Natural polyelectrolytes, including in the form of complexes with colloidal particles, are increasingly used in pharmacy due to the possibility of regulated attachment of medicinal substances and ...their targeted delivery to the target organ. However, the formation, stability, and molecular-mass characteristics of polyelectrolyte nanodispersions (ND) vary depending on the nature and composition of the medium of their origin. This is due to the lack of standardized approaches to quality control and regulatory documentation for most natural ND. In this paper, we first introduced the isolation, followed by investigations into their physico-chemical properties and bioactivity. Using the dried droplet method, we were able to detect the “coffee ring effect”. Fractographic studies of the surface structure of EHA and FA dried samples using SEM showed its heterogeneity and the presence of submicron particles encapsulated in the internal molecular cavities of polyelectrolyte. FTIR spectroscopy revealed the ND chemical structure of benzo-α-pyron and benzo-γ-pyron, consisting of nanoparticles and a branched frame part. The main elements detected by X-ray fluorescence in humic substance extract and fulvic acid include Si, P, S, K, Ca, Mn, Fe, Cu, Zn, whereas Fe is in high concentrations. The UV-spectra and fluorescent radiation demonstrated the possibility of studying the effect of the fulvate chromone structure on its optical properties. It is shown that dilution of the initial solutions of polyelectrolytes 1:10 contributes to the detection of smaller nanoparticles and an increase in the absolute value of the negative ζ-potential as a factor of ND stability. A study of the EHS effect on the SARS-CoV-2 virus infectious titer in the Vero E6 cell showed the effective against virus both in the virucidal scheme (the SI is 11.90–22.43) and treatment/prevention scheme (the SI is 34.85–57.33). We assume that polyelectrolyte ND prevent the binding of the coronavirus spike glycoprotein to the receptor. Taking into account the results obtained, we expect that the developed approach can become unified for the standardization of the ND natural polyelectrolytes complex, which has great prospects for use in pharmacy and medicine as a drug with antiviral activity.
•The full genome sequence of the subtype 2 PRRSV-1 WestSib13 strain is determined.•Demonstrated significant difference in pathogenicity between subtype 1 and 2 strains of European PRRSV.•Demonstrated ...the unusual virological and clinical outcome (high level viremia without fever) of the novel WestSib13 strain.
Porcine reproductive and respiratory syndrome virus (PRRSV) causes reproductive failure and respiratory problems. Data about the virulence and pathogenicity of subtype 2 PRRSV-1 strains are limited. The main purposes of this investigation were to characterize the full genome sequence of the subtype 2 PRRSV-1 WestSib13 strain and to compare the pathogenicity with that of the subtype 1 PRRSV-1 Lelystad strain. Comparison of the whole genome sequence of the WestSib13 strain with that of PRRSV-1 prototype strains revealed a 76.2% (subtype 1 Lelystad virus) and 79.0% (subtype 3 Lena virus) identity, respectively
The virulence and pathogenicity of the European subtype 2 PRRSV strain WestSib13 and the European subtype 1 PRRSV strain Lelystad were compared in 3-week-old piglets upon inoculation of 105.4 TCID50 of virus. Non-infected animals (control group) as well as animals infected with the Lelystad strain were clinically healthy until 14days post challenge. In contrast, animals infected with the WestSib13 strain demonstrated dyspnea starting at 3days post-inoculation (dpi). All piglets in this group died between 5 and 8 dpi. During that period, fever was not observed in WestSib13-infected animals. Viremia was detected in animals from both infected groups starting from 2 dpi. Viral loads in serum and lungs upon euthanasia were significantly higher (3 log10) in the WestSib13-infected than in the LV-infected animals.
Taken together, this study provides the full genome sequence and the unusual virological and clinical outcome (high level viremia without fever) of the novel WestSib13 strain.
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