This experiment was designed to explore the changes in serum Se selectin, ACTH, LPS and SIRT1 levels in patients with acute pancreatitis and their correlation with the severity of the disease. To do ...this research, from March 2019 to December 2020, 86 patients with different degrees of acute pancreatitis were selected as the research object. They were divided into mild acute pancreatitis (MAP) group (n = 43), moderately severe acute pancreatitis and severe acute pancreatitis (MSAP + SAP) group (n = 43) and healthy group (n = 43). At the same time, the serum levels of Se selectin, ACTH, LPS and SIRT1 were detected after hospitalization. Results showed that the levels of serum Se selectin, ACTH and SIRT1 in the MAP group and MSAP + SAP group were lower than those in the healthy group; The LPS of the MAP group and MSAP + SAP group were higher than that of the healthy group. The results showed that serum Se selectin, ACTH and SIRT1 decreased with the development of the disease, showing a negative correlation; The change of LPS in patients will increase with the development of the disease, showing a positive correlation. Serum Se selectin, ACTH, SIRT1, and LPS can be used as diagnostic criteria and indicators of acute pancreatitis to achieve early prevention and treatment and improve the prognosis and quality of life of patients.
Art major graduates are facing more severe employment pressure. Based on entrepreneurial education, the reform of entrepreneurship education and quality training of music majors in colleges and ...universities are analyzed. First, the relevant theories of entrepreneurship education are introduced and the advantages of advanced entrepreneurship education in foreign countries are analyzed. Second, the music majors in a university are selected as the subjects to analyze the current situation of entrepreneurship of music majors. Finally, new strategies are put forward for the reform of entrepreneurship education of music majors. The research shows that the number of music majors who are very interested in entrepreneurship accounts for 22.2%. In terms of the music majors' understanding of entrepreneurship policies, the proportion of students with less understanding accounts for 40.1%. As for the ways of music majors to acquire entrepreneurship knowledge, 8.00% usually turn to books and networks. Music majors hope to get support from the government and schools in the cultivation of entrepreneurship. In entrepreneurship, music majors who intend to start a business according to their interests account for 40.8%. The variance of eigenvalues of the four main factors is 19.49, 12.96, 10.75, and 8.39%, respectively, and their contribution value is 51.58%. The four research aspects of entrepreneurship education are music majors' entrepreneurship policy, entrepreneurship desire, and entrepreneurship quality and entrepreneurship education practice. The entrepreneurship education system is to arouse interest, improve entrepreneurial ability, and form entrepreneurial personality. This paper proposes good entrepreneurship education strategies.
The significance of fatty acid metabolism in cancer initiation and development is increasingly accepted by scientists and the public due to the high prevalence of overweight and obese individuals. ...Fatty acids have different turnovers in the body: Either breakdown into acetyl-CoA to aid ATP generation through catabolic metabolism or incorporation into triacylglycerol and phospholipid through anabolic metabolism. However, these two distinct pathways require a common initial step known as fatty acid activation. Long-chain acyl-CoA synthetases (ACSLs), which are responsible for activation of the most abundant long-chain fatty acids, are commonly deregulated in cancer. This deregulation is also associated with poor survival in patients with cancer. Fatty acids physiologically regulate ACSL expression, but cancer cells could hijack certain involved regulatory mechanisms to deregulate ACSLs. Among the five family isoforms, ACSL1 and ACSL4 are able to promote ungoverned cell growth, facilitate tumor invasion and evade programmed cell death, while ACSL3 may have relatively complex functions in different types of cancer. Notably, ACSL4 is also essential for the induction of ferroptosis (another form of programmed cell death) by facilitating arachidonic acid oxidation, which makes the enzyme a desirable cancer target. The present review thus evaluates the functions of deregulated ACSLs in cancer, the possible molecular mechanisms involved and the chemotherapeutic potentials to target ACSLs. A better understanding of the pathological effects of ACSLs in cancer and the involved molecular mechanisms will aid in delineating the exact role of fatty acid metabolism in cancer and designing precise cancer prevention and treatment strategies.
This paper applies the cluster analysis method to classify different Civics contexts in university English courses, and improves the accuracy of Civics context text clustering by extracting the word ...stems of English Civics context texts. The text similarity calculation is used to remove feature words from the preprocessed text, to converge into a collection of feature words. The texts containing English Civic and Political Context are clustered and analyzed, and the clustering effect of Civic and Political Context texts is judged according to the clustering evaluation. The status quo of Civic and Political integration into English curriculum is explored from different dimensions, and corresponding optimization strategies are proposed with the converged data. The results show that the Civic-Political contexts of English courses are mainly divided into three categories, and based on the clustering of the Civic-Political contexts of university English courses, the contexts of “living, learning and doing” are relatively independent. The contexts “history, culture and society” and “science and technology” are closer. In integrating Civics and Politics into English teaching, the mean value of the dimension of teaching method is 4.212, which is in a better integration situation.
•A water-soluble galactoglucan (PCP-1C) was purified from Poria cocos.•The chemical composition and structural features of PCP-1C were characterized.•PCP-1C can effectively inhibit the metabolism of ...toxicant and reduce liver damage.
To find the polysaccharide with hepatoprotective activity from Poria cocos and clarify its structure, a galactoglucan (PCP-1C) with a molecular weight of 17 kDa was purified from the Poria cocos sclerotium by column chromatography and activity evaluation in the present work. It was composed of galactose, glucose, mannose, and fucose in a molar percentage of 43.5: 24.4: 17.4: 14.6. Structural characterization showed that PCP-1C has a backbone consisted of 1,6-α-D-Galp, which branches composed of 1,3-β-D-Glcp, 1,4-β-D-Glcp, 1,6-β-D-Glcp, T-β-D-Glcp, T-α-D-Manp, T-α-L-Fucp and 1,3-α-L-Fucp. In vivo experiments found that PCP-1C can apparently improve the damage of liver tissue in CCl4-treated mice and relieve oxidative stress and inflammation. PCP-1C also reduced the expression of CAR and CYP2E1 in the liver. These findings indicated strong hepatoprotective effect of PCP-1C, which was attributed to the reduction of CCl4 metabolism via inhibiting the CAR/CYP2E1 signal pathway.
Abstract
Long-read sequencing is promising for the comprehensive discovery of structural variations (SVs). However, it is still non-trivial to achieve high yields and performance simultaneously due ...to the complex SV signatures implied by noisy long reads. We propose cuteSV, a sensitive, fast, and scalable long-read-based SV detection approach. cuteSV uses tailored methods to collect the signatures of various types of SVs and employs a clustering-and-refinement method to implement sensitive SV detection. Benchmarks on simulated and real long-read sequencing datasets demonstrate that cuteSV has higher yields and scaling performance than state-of-the-art tools. cuteSV is available at
https://github.com/tjiangHIT/cuteSV
.
Mechanoluminescence (ML) featuring photon emission by mechanical stimuli is promising for applications such as stress sensing, display, and artificial skin. However, the progress of utilizing ML ...processes is constrained by the limited range of available ML emission spectra. Herein, a general strategy for expanding the emission of ML through the use of lanthanide emitters is reported. A lithium‐assisted annealing method for effective incorporation of various lanthanide ions (e.g., Tb3+, Eu3+, Pr3+, Sm3+, Er3+, Dy3+, Ho3+, Nd3+, Tm3+, and Yb3+) into CaZnOS crystals that are identified as one of the most efficient host materials for ML is developed. These doped CaZnOS crystals show efficient and tunable ML spanning full spectrum from violet to near infrared. The multicolor ML materials are used to create encrypted anticounterfeiting patterns, which produce spatially resolvable optical codes under single‐point dynamic pressure of a ballpoint pen.
Multicolor mechanoluminescence spanning the full spectrum from violet to near infrared is achieved, based on effective incorporation of a wide collection of lanthanide ions into CaZnOS crystals through a lithium‐assisted annealing method. Multicolor mechanoluminescent materials are used to create encrypted anticounterfeiting patterns, which produce spatially resolvable optical codes under mechanical excitation.
Non-Hermitian optical systems with parity-time (PT) symmetry have recently revealed many intriguing prospects that outperform conservative structures. The previous works are mostly rooted in complex ...arrangements with controlled gain-loss interplay. Here, we demonstrate anti-PT symmetry inherent in the nonlinear optical interaction based upon forward optical four-wave mixing in a laser-cooled atomic ensemble with negligible linear gain and loss. We observe that the pair of frequency modes undergo a nontrivial anti-PT phase transition between coherent power oscillation and optical parametric amplification in presence of a large phase mismatch.
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CMK, CTK, FMFMET, NUK, UL
Tribulus terrestris L. (Zygophyllaceae) (TT) is usually used as a cardiotonic, diuretic, and aphrodisiac, as well as for herbal post-stroke rehabilitation in traditional Chinese medicine. However, ...little is known about the renoprotective effects of TT on obesity-related glomerulopathy (ORG). In this study, 340 monomeric compounds were identified from TT extracts obtained with ethyl acetate combined with 50% methanol. In vitro, IC50 of TT was 912.01 mg/L, and the appropriate concentration of TT against oxidized-low density lipoprotein (ox-LDL) induced human renal glomerular endothelial cells (HRGECs) was 4 mg/L. TT significantly increased the viability (63.2%) and migration (2.33-fold increase) of HRGECs. ORG model rats were induced by a chronic high-fat diet (45%) for 20 weeks and were then treated with TT extract (2.8 g/kg/d) for 8 weeks. Subsequently, the kidneys were removed and their differentially expressed protein profile was identified using two-dimensional electrophoresis coupled with matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF)-TOF MS. Molecular categorization and functional analysis of bioinformatic annotation suggested that excessive energy metabolism, decreased response to stress and low immunity were the potential etiologies of ORG. After TT administration for 8 weeks, body weight, blood pressure, serum cystatin C and cholesterol were decreased. Additionally, TT significantly enhanced the resistance of rats to ORG, decreased energy consumption and the hemorrhagic tendency, and improved the response to acute phase reactants and immunity. In conclusion, TT may play a protective role against ORG in rats.
Endothelial senescence is an important risk factor leading to atherosclerosis. The mechanism of quercetin against endothelial senescence is worth exploring.
Quercetin (20 mg/kg/d) was administered to ...ApoE
mice intragastrically to evaluate the effectiveness of quercetin on atherosclerotic lesion
.
, human aortic endothelial cells (HAECs) were used to assess the effect of quercetin on cellular senescence induced by oxidized low-density lipoprotein (ox-LDL). Transcriptome microarray and quantitative RT-PCR was conducted to study the pharmacological targets of quercetin.
ApoE
mice demonstrated obvious lipid deposition in arterial lumina, high level of serum sIcam-1 and IL-6, and high density of Vcam-1 and lower density of Sirt1 in aorta. Quercetin administration decreased lipid deposition in arterial lumina, serum sIcam-1, and IL-6 and Vcam-1 in aorta, while increased the density of Sirt1 in aorta of ApoE
mice.
, quercetin (0.3, 1, or 3 μmol/L) decreased the expression of senescence-associated β-galactosidase and improved cell morphology of HAECs. And quercetin decreased the cellular apoptosis and increased mitochondrial membrane potential (ΔΨm) in dose-dependent manner, and decreased ROS generation simultaneously. Transcriptome microarray suggested 254 differentially expressed (DE) mRNAs (110 mRNAs were upregulated and 144 mRNAs were downregulated) in HAECs after quercetin treatment (fold change > 1.5,
< 0 .05, Que
Ox-LDL). GO and KEGG analysis indicated nitrogen metabolism, ECM-receptor interaction, complement, and coagulation cascades, p53 and mTOR signaling pathway were involved in the pharmacological mechanisms of quercetin against ox-LDL.
Quercetin alleviated atherosclerotic lesion both
and
.
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