LETTERS TO THE EDITOR Richard F. Edlich, MD, PhD; William B. Long III, MD; Greene, Jill Amanda ...
Journal of the American Geriatrics Society (JAGS),
10/2007, Volume:
55, Issue:
10
Journal Article
Some of the Fourth Amendment issues related to the use of thermal imaging devices (TIDs) to find and prosecute criminals are examined. The courts are still split on what to do with TID evidence, and ...how to frame the important Fourth Amendment questions that will make or break a defendant's case.
Structure of a cyclin-dependent kinase from Giardia lamblia Leibly, David J.; Newling, Paul A.; Abendroth, Jan ...
Acta crystallographica. Section F, Structural biology and crystallization communications,
September 2011, Volume:
67, Issue:
9
Journal Article
Peer reviewed
Open access
Giardia lamblia is the etiologic agent of giardiasis, a water‐borne infection that is prevalent throughout the world. The need for new therapeutics for the treatment of giardiasis is of paramount ...importance. Owing to the ubiquitous nature of kinases and their vital importance in organisms, they are potential drug targets. In this paper, the first structure of a cyclin‐dependent kinase (CDK) from G. lamblia (GlCDK; UniProt A8BZ95) is presented. CDKs are cell‐cycle‐associated kinases that are actively being pursued as targets for anticancer drugs as well as for antiparasitic chemotherapy. Generally, a CDK forms a complex with its associated cyclin. This CDK–cyclin complex is active and acts as a serine/threonine protein kinase. Typically, CDKs are responsible for the transition to the next phase of the cell cycle. Although the structure of GlCDK with its associated cyclin was not solved, the 1.85 Å resolution structure of apo GlCDK and a 2.0 Å resolution structure of GlCDK in complex with adenosine monophosphate are presented and the structural differences from the orthologous human CDK2 and CDK3 are discussed.
This randomized controlled trial compared 3 modes of training clinicians in a youth-focused evidence-based treatment for panic disorder, using an additive design. Community practitioners (N = 140) ...were randomly assigned to one of three conditions: a standard text-alone training condition (TXT); an interactive, online training program plus the TXT (TXT + OLT); or the TXT + OLT enhanced by a learning community adjunct leveraging Twitter (TXT + OLT + LC). Mastery of Anxiety and Panic for Adolescents (MAP-A), an efficacious cognitive-behavioral therapy for adolescents with panic disorder, was taught in all three training conditions. Analyses revealed that participants were more satisfied with the TXT + OLT and TXT + OLT + LC training, compared to the TXT condition. Although all conditions demonstrated equivalent increases in knowledge of MAP-A, participants in the TXT + OLT + LC demonstrated greater proficiency using MAP-A skills in a simulated clinical scenario, compared to those in both the TXT + OLT and TXT conditions. However, this effect was no longer statistically significant at a 90-day follow-up. Results indicate initial promise for enhancing online training dissemination efforts with a novel learning community and Twitter adjunct in training clinicians in a youth-focused, efficacious cognitive-behavioral therapy. Directions for future research and dissemination efforts are discussed.
Structure of triosephosphate isomerase from Cryptosporidium parvum Nguyen, Trang N.; Abendroth, Jan; Leibly, David J. ...
Acta crystallographica. Section F, Structural biology and crystallization communications,
September 2011, Volume:
67, Issue:
9
Journal Article
Peer reviewed
Open access
Cryptosporidium parvum is one of several Cryptosporidium spp. that cause the parasitic infection cryptosporidiosis. Cryptosporidiosis is a diarrheal infection that is spread via the fecal–oral route ...and is commonly caused by contaminated drinking water. Triosephosphate isomerase is an enzyme that is ubiquitous to all organisms that perform glycolysis. Triosephosphate isomerase catalyzes the formation of glyceraldehyde 3‐phosphate from dihydroxyacetone phosphate, which is a critical step to ensure the maximum ATP production per glucose molecule. In this paper, the 1.55 Å resolution crystal structure of the open‐loop form of triosephosphate isomerase from C. parvum Iowa II is presented. An unidentified electron density was found in the active site.
Structure of thymidylate kinase from Ehrlichia chaffeensis Leibly, David J.; Abendroth, Jan; Bryan, Cassie M. ...
Acta crystallographica. Section F, Structural biology and crystallization communications,
September 2011, Volume:
67, Issue:
9
Journal Article
Peer reviewed
Open access
The enzyme thymidylate kinase phosphorylates the substrate thymidine 5′‐phosphate (dTMP) to form thymidine 5′‐diphosphate (dTDP), which is further phosphorylated to dTTP for incorporation into DNA. ...Ehrlichia chaffeensis is the etiologic agent of human monocytotropic erlichiosis (HME), a potentially life‐threatening tick‐borne infection. HME is endemic in the United States from the southern states up to the eastern seaboard. HME is transmitted to humans via the lone star tick Amblyomma americanum. Here, the 2.15 Å resolution crystal structure of thymidylate kinase from E. chaffeensis in the apo form is presented.
Vitelline membrane strength plays a role in preventing contamination of albumen by yolk during separation and is important to food safety. Two experiments were conducted to determine if a ...relationship exists between vitelline membrane protein banding patterns, interior egg quality, and vitelline membrane rupture strength. Eggs were gathered from commercial egg producers from pre-molt (26 wk or 72 wk) and post-molted (74 wk or 82 wk) hens. In each of two trials twenty-one eggs were gathered and stored (4°C) per experiment. Three eggs were evaluated on days 0, 7, 14, 21, 28, 35, and 42 for eggs from pre-molted hens; and 1, 7, 14, 21, 28, 35, and 42 for eggs from molted hens for changes in SDS-PAGE protein banding patterns. The yolk from each egg was isolated and rolled on a wet paper towel to remove adhering albumen. The yolk was emptied and washed. The whole membrane was placed into double deionized water and divided into two sections. The first section was the whole membrane sample and the other was separated by forceps into inner and outer membrane samples. The three sections were dissolved separately in 1% SDS/70 mM Tris/HCl, pH 6.8. Protein concentration was determined using the Lowry method and proteins separated on 4-20% gradient gel by SDS-PAGE. Protein banding patterns were analyzed using the Bio-Rad Multi-Analyst Densitometer. Reductions of VMO I and GP II occurred along with reductions in the protein bands between 60 to 100 kDa.
In each of two trials, an additional one hundred forty eggs were gathered at the same time from the same flock and stored at 4°C. Twenty eggs were evaluated for quality on days 0, 7, 14, 21, 28, 35, and 42 for eggs from pre-molted hens; and 1, 7, 14, 21, 28, 35, and 42 for eggs from molted hens. Yolk index, albumen height, albumen pH, and yolk pH were determined. Vitelline membrane strength was determined using a compression anvil. Two different treatments were used on the yolk when evaluating rupture strength: 10 egg yolks with inner thin albumen layer, and 10 egg yolks rolled on wet paper towel to remove inner thin albumen layer. Interior egg quality and vitelline membrane strength declined during storage.
Thesis (M.S.)--Texas A & M University, 2003.
"Major Subject: Food Science and Technology." Title from author supplied metadata (automated record created on Apr. 30, 2004.). Vita. Abstract. Includes ...bibliographical references.
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