Background: Colorectal cancer (CRC) is currently the third most common cancer type in males and the second most occurring in females. The role of microRNA (miRNA) in the development of colorectal ...cancer is not fully elucidated. Therefore, understanding the mechanistic interaction between miRNA and their target oncogenes may hold great importance as a possible target for interventional anticancer therapy.
Aims: To identify miRNAs that are part of the regulating pathway of Monocarboxylate Transporter-4 (MCT4) and Vascular Endothelial Growth Factor (VEGF) oncogenes.
Study Design: We used publicly available prediction tools (e.g. TargetScan, MicroCosm, PicTar, and DIANA-microT-CDS) to identify the possible miRNA that target the two oncogenes.
Methodology: We used the GeneMania database to visualize the network and verify gene names and remove ambiguity and duplications. Furthermore, we used miRTarBase database to identify experimentally validated targets which we used to further confirm miRNA-oncogene relationships. Finally, we utilized miR-Mfold web-tool to further visualize the circular structures and the simulated miR-1 and miR-206 targeting arrangements.
Results: We found two putative miRNA (miR-1 and miR-206) that may downregulate MCT4 coded by SLC16A3 gene and VEGF which is coded by VEGF gene. We found relationships between the validated target genes of miR-1 and miR-206 through GeneMania which we extracted from the literature. And we elucidated the proposed structure of these two miRNAs through miR-Mfold web-tool.
Conclusion: Our results elucidated a novel regulation pathway in CRC cells and may suggest a potential therapeutic approach for CRC therapy. MiR-1 and miR-206 may help cells go to apoptosis and inhibit the angiogenesis of colorectal cancer cells by down-regulation of MCT4 and VEGF proteins in tumor tissues.
During the COVID-19 pandemic, panic and public scare spread among individuals including healthcare providers, and a need to design an alternative clinical training away from hospitals where there ...were many COVID-19 infections became a top priority. This study aimed to implement special clinical training for pharmacy students during the COVID-19 pandemic. Clinical training was conducted in the University of Petra (UOP) campus clinic instead of hospitals. Campus training was from July to September 2020. The in-campus clinical training at the UOP clinic involved primary patient care concentrating on pharmaceutical care, which focuses on correct drug indication, effectiveness, safety, and adherence. A total of 15 final years pharmacy students from the faculty of pharmacy finished the post-training questionnaire. Qualitative responses from students exhibited positive answers linked to campus clinical training orientation, preceptors, and pharmacy students’ clinical knowledge and clinical skills. Students expressed positive and high appraisals of clinical training locations and medical staff/preceptors.
Keywords: Clinic; Coronavirus; COVID-19; education; pharmacy; training.
During the COVID-19 pandemic, panic and public scare spread among individuals including healthcare providers, and a need to design an alternative clinical training away from hospitals where there ...were many COVID-19 infections became a top priority. This study aimed to implement special clinical training for pharmacy students during the COVID-19 pandemic. Clinical training was conducted in the University of Petra (UOP) campus clinic instead of hospitals. Campus training was from July to September 2020. The in-campus clinical training at the UOP clinic involved primary patient care concentrating on pharmaceutical care, which focuses on correct drug indication, effectiveness, safety, and adherence. A total of 15 final years pharmacy students from the faculty of pharmacy finished the post-training questionnaire. Qualitative responses from students exhibited positive answers linked to campus clinical training orientation, preceptors, and pharmacy students’ clinical knowledge and clinical skills. Students expressed positive and high appraisals of clinical training locations and medical staff/preceptors.
Colorectal cancer is one of the most common types of cancer in the world, and its incidence is mostly influenced by lifestyle factors. Despite having a much smaller role, genetics also affects the ...susceptibility and development of colorectal cancer. The aim of the present study is to investigate the regulatory functions of candidate microRNAs (miRs) 1 and 206 in the context of solute carrier family 16 member 3 (SLC16A3) and vascular endothelial growth factor (VEGF) expression. To achieve this, 24 oncogenes targeted by miR-1 and miR-206 were analyzed via GeneMANIA. The miRTarBase database was then employed to ascertain the nature of the miR-oncogene relationship. Our findings illustrate that miR-1/206 indirectly reduce CRC growth and infiltration by targeting the both the SLC16A3 and VEGF genes. Moreover, miR-1/206 targets the VEGF gene to reduce tumor angiogenesis and vasculature. Conclusively, the results of the current study illustrate a novel regulation pathway in CRC cells, suggesting new potential lines of CRC therapy.
碩士
國立交通大學
生物資訊及系統生物研究所
100
Ovarian Cancer is the main cause of gynecological related cancer deaths worldwide. The major purpose of this work is to put some light on the most recent findings and ...studies regarding ovarian cancer pathogenesis (initiation and progression) on the molecular level, in particular microRNAs (miRNAs or miRs), a class of non-coding RNAs that post-transcriptionally regulate gene expression and their target genes involved in ovarian cancer. Both play a major role in ovarian tumorigenesis. Many miRNA expression profiling surveys have revealed changes in miRNA patterns that are involved during ovarian cancer development. Most of these deregulated miRNAs are down-regulated that could act as tumor suppressors, while the others are aberrantly up-regulated which represent oncogenes. Among these patterns, some could serve as prognostic signature as well as diagnostic value, others may have important role in survival outcome, chemoresistance, invasiveness and progression. Although much still to be elucidated in this area, better understanding of miRNAs and their targets could provide useful interpretation for the prognosis, diagnosis and future therapy for this vicious disease.
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