The developed mathematical model was applied for study of fluid dynamics in a rotational bioreactor for bone tissue engineering by
in vitro
technology. The research goal is finding an optimal mode ...for rotation ensuring proper cyclic loading from fluid upon the cell-seeded biomaterial. The basis for developing a mathematical model of a bioreactor was a design of rotational type biological reactor used in medical research; the liquid flow is generated through viscosity mechanism due to surface rotation. Mathematical description of flow in a reactor cavity was performed with Navier—Stokes equations. It was assumed that flow regime in the boundary layer is laminar. Numerical algorithm was accomplished using a fluid flow solver “Fluent” in the code package ANSYS-12. Four variants of generating the rotational motion in the reactor cavity were considered. A series of parametric computations was performed for the rotation frequency
f
in the range 0.05 ≤
f
≤ 0.25 Hz. The paper offers visualization of velocity fields in the vertical plane. The distributions for shear stress and pressure in the working zone of reactor were calculated and analyzed. Simulations demonstrated that a method of fluid rotation by driving the outer cylinder with an offset axis is the best for arranging a cyclic pressure and cyclic shear stress on the biological material.
Cheese makers conduct research to improve the recipe and technology of cheese production. In Russia, the main raw material for cheese production is cow's milk. The aim of the research is to study the ...cheese suitability of milk and to improve the technology for production of soft cheese 'Academicheskiy'. The physical and chemical properties of milk were studiedby ultrasound method. The results of studies of milk quality were taken into account in developing the recipe and production technology of soft cheese. The cheese recipe included cow's milk, sour milk whey and salt. Soft cheese was made by hand using a thermal-acid method. The production technology of soft cheese 'Academicheskiy' consists of the following operations: acceptance of milk and additional raw materials; processing and preparation of milk; pasteurization of milk and sour whey at high temperature; mixing of milk and sour whey for protein precipitation; molding and self-pressing; salting; drying and maturation; packaging and labeling; storage, transportation and sale of soft cheese.
We explore the variability and cross-frequency correlation of the flux density and polarization of the blazar OJ287, using imaging at 43 GHz with the Very Long Baseline Array, as well as optical and ...near-infrared (near-IR) polarimetry. The polarization and flux density in both the optical waveband and the 43 GHz compact core increased by a small amount in late 2005, and increased significantly along with the near-IR polarization and flux density over the course of 10 days in early 2006. Furthermore, the values of the electric vector position angle (EVPA) at the three wavebands are similar. At 43 GHz, the EVPA of the blazar core is perpendicular to the flow of the jet, while the EVPAs of emerging superluminal knots are aligned parallel to the jet axis. The core polarization is that expected if shear aligns the magnetic field at the boundary between flows of disparate velocities within the jet. Using variations in flux density, percentage polarization, and EVPA, we model the inner jet as a spine-sheath system. The model jet contains a turbulent spine of half-width 12 and maximum Lorentz factor of 16.5, a turbulent sheath with Lorentz factor of 5, and a boundary region of sheared field between the spine and sheath. Transverse shocks propagating along the fast, turbulent spine can explain the superluminal knots. The observed flux density and polarization variations are then compatible with changes in the direction of the inner jet caused by a temporary change in the position of the core if the spine contains wiggles owing to an instability. In addition, we can explain a stable offset of optical and near-IR percentage polarization by a steepening of spectral index with frequency, as supported by the data.
Since the launch of the Fermi satellite, BL Lacertae has been moderately active at gamma-rays and optical frequencies until May 2011, when the source started a series of strong flares. The ...exceptional optical sampling achieved by the GLAST-AGILE Support Program (GASP) of the Whole Earth Blazar Telescope (WEBT) in collaboration with the Steward Observatory allows us to perform a detailed comparison with the daily gamma-ray observations by Fermi. Discrete correlation analysis between the optical and gamma-ray emission reveals correlation with a time lag of 0 +- 1 d, which suggests cospatiality of the corresponding jet emitting regions. A better definition of the time lag is hindered by the daily gaps in the sampling of the extremely fast flux variations. In general, optical flares present more structure and develop on longer time scales than corresponding gamma-ray flares. Observations at X-rays and at millimetre wavelengths reveal a common trend, which suggests that the region producing the mm and X-ray radiation is located downstream from the optical and gamma-ray-emitting zone in the jet. The mean optical degree of polarisation slightly decreases over the considered period and in general it is higher when the flux is lower. The optical electric vector polarisation angle (EVPA) shows a preferred orientation of about 15 deg, nearly aligned with the radio core EVPA and mean jet direction. Oscillations around it increase during the 2011-2012 outburst. We investigate the effects of a geometrical interpretation of the long-term flux variability on the polarisation. A helical magnetic field model predicts an evolution of the mean polarisation that is in reasonable agreement with the observations. These can be fully explained by introducing slight variations in the compression factor in a transverse shock waves model.
A biotechnological system for the production of human beta interferon was developed on the basis of a hybrid gene constructed from the coding sequence of the beta interferon gene inserted into the ...first exon of the sheep beta lactoglobulin gene. It is intended for the expression of human beta interferon in mammary glands of transgenic animals. Two lines of transgenic rabbits were obtained using the hybrid gene. The tissue specificity of the expression of the transgene and the frequency of its inheritance in the first and second generations were studied. The activity of interferon was 2.2 x 10(4)-7.2 x 10(4) IU per milliliter of milk of transgenic female rabbits. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2008, vol. 34, no. 2; see also http:// www.maik.ru.
—The processes of hydrogen migration in a titanium plate and inhomogeneous hydrogen saturation are studied by measuring the thermopower and eddy currents. The processes of inhomogeneous hydrogen ...saturation of commercial-purity VT1-0 titanium are developed, tested, and investigated using these methods of nondestructive testing. To achieve an inhomogeneous hydrogen concentration in a titanium plate, a TiN film 1–2.6 μm thick is deposited onto it by magnetron sputtering, and the parts of the plate free of the film are hydrogenated. The thermo-emf and eddy currents are measured at various probe coordinates before and after hydrogen saturation of the plate. Eddy current testing demonstrates that, after the plate is hydrogen saturated for 1.5 h, the thermo-emf on the side of hydrogen sorption is significantly lower than on the coated side. After 144 h, a uniform thermo-emf distribution is obtained, as follows from eddy current measurements. The thermo-emf study is performed upon heating at a constant rate. The thermo-emf of each part of the titanium alloy surface (including the uncoated part) is approximately equal to the value obtained before hydrogenation. The electrical resistivity of the coated titanium plate at room temperature is approximately 0.315 mΩ cm. After hydrogenation, the hydrogen content in the uncoated part decreases slowly. The thermo-emf also decreases gradually. A hydrogen content distribution can be formed via a thermo-emf distribution in the titanium plate. After holding for a sufficiently long time, a uniform thermo-emf distribution forms in the plate.
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