We aimed to evaluate the clinical impact of shift patterns at work on gynecologic problems and the healthcare behavior of Korean nursing staff. We conducted a web-based survey for over two weeks in ...September 2021, involving female nursing staff, including nurses, nurses’ aides, and nursing assistants, working in five medical centers. The questionnaire included 40 questions on baseline characteristics, working information, and experiences with gynecologic problems and medical approaches. Overall, 885 nursing staffs participated in the survey, of the 1,904 who received the invitation with an online link of the survey. The response rate was 46.5%. Among the participants responding to all questions, 569 (64.4%) worked two or three shifts and 305 (34.5%) worked full-time. In women rotating two or three-shift patterns, irregular menstrual cycles (21.6% vs. 13.8%, p = 0.005), abnormal menstrual cycles (40.9% vs. 33.8%, p = 0.038), and dysmenorrhea (48.0% vs. 38.4%, p = 0.006) were more frequent than in those who worked full-time. The experience of visiting gynecologic clinics (47.5% vs. 44.1%, p = 0.332) and treating gynecologic conditions (33.4% vs. 29.3%, p = 0.211) did not differ according to the working patterns. However, diagnosis of gynecologic diseases was more frequent in women working full-time (36.4% vs. 29.7%, p = 0.043). The rates of screening (76.1% vs. 57.8%, p<0.001) and human papillomavirus vaccination (55.7% vs. 39.9%, p<0.001) for cervical cancer prevention were higher in women working full-time than in two–three shifts. This study showed that rotating shift work may be related to an increase in gynecologic problems and a decrease in cancer prevention activity among female nursing staff.
The coronavirus 2019 (COVID-19) has brought significant changes to college students, but there is a lack of empirical studies regarding how the pandemic has affected student mental health among ...college students in the U.S. To fill the gap in the literature, this study describes stress, anxiety, and depression symptoms for students in a public research university in Kentucky during an early phase of COVID-19 and their usage of mental health services. Results show that about 88% of students experienced moderate to severe stress, with 44% of students showing moderate to severe anxiety and 36% of students having moderate to severe depression. In particular, female, rural, low-income, and academically underperforming students were more vulnerable to these mental health issues. However, a majority of students with moderate or severe mental health symptoms never used mental health services. Our results call for proactively reaching out to students, identifying students at risk of mental health issues, and providing accessible care.
COVID-19 is a respiratory disease caused by a novel coronavirus that quickly spread worldwide, resulting in a global pandemic. Healthcare professionals coming into close contact with COVID-19 ...patients experience mental health issues, including stress, depression, anxiety, post-traumatic stress disorder, and burnout. This study aimed to explore the experiences of COVID-19-designated hospital nurses in South Korea who provided care for patients based on their lived experiences. Eighteen nurses working in a COVID-19-designated hospital completed in-depth individual telephone interviews between July and September 2020, and the data were analyzed using Giorgi's phenomenological methodology. The essential structure of the phenomenon was growth after the frontline battle against an infectious disease pandemic. Nine themes were identified: Pushed onto the Battlefield Without Any Preparation, Struggling on the Frontline, Altered Daily Life, Low Morale, Unexpectedly Long War, Ambivalence Toward Patients, Forces that Keep Me Going, Giving Meaning to My Work, and Taking Another Step in One's Growth. The nurses who cared for patients with COVID-19 had both negative and positive experiences, including post-traumatic growth. These findings could be used as basic data for establishing hospital systems and policies to support frontline nurses coping with infectious disease control to increase their adaption and positive experiences.
Abstract
Kinematic mechanisms of the Pacific–North America (PNA)-like teleconnection pattern induced by the Madden–Julian oscillation (MJO) is examined using an atmospheric general circulation model ...(GCM) and a barotropic Rossby wave theory. Observation shows that a negative PNA-like teleconnection pattern emerges in response to MJO phase-2 forcing with enhanced (suppressed) convection located over the Indian (western Pacific) Ocean. The GCM simulations show that both forcing anomalies contribute to creating the PNA-like pattern. Indian Ocean forcing induces two major Rossby wave source (RWS) regions: a negative region around southern Asia and a positive region over the western North Pacific (WNP). The negative RWS to the north of the enhanced convection in the Indian Ocean arises from southerly MJO-induced divergent wind crossing the Asian jet. Unexpectedly, another significant RWS region develops over the WNP owing to refracted northerly divergent wind. A ray-tracing method demonstrates three different ways of wave propagation emanating from the RWS to the PNA region: 1) direct arclike propagation from the negative RWS to the PNA region occurs in the longest waves, 2) shorter waves are displaced first downstream by the jet waveguide effect and then emanate at the jet exit to the PNA region, and 3) waves with zonal wavenumbers 1 and 2 exhibit canonical wave propagation from the positive RWS at the jet exit to the PNA region.
On the other hand, the positive RWS induced by western Pacific forcing shows similar characteristics to feature 3 described above, with some relaxation such that much shorter waves also contribute to the formation of the southern cells.
Investigation of neural circuit dynamics is crucial for deciphering the functional connections among regions of the brain and understanding the mechanism of brain dysfunction. Despite the ...advancements of neural circuit models in vitro, technologies for both precisely monitoring and modulating neural activities within three-dimensional (3D) neural circuit models have yet to be developed. Specifically, no existing 3D microelectrode arrays (MEAs) have integrated capabilities to stimulate surrounding neurons and to monitor the temporal evolution of the formation of a neural network in real time. Herein, we present a 3D high-density multifunctional MEA with optical stimulation and drug delivery for investigating neural circuit dynamics within engineered 3D neural tissues. We demonstrate precise measurements of synaptic latencies in 3D neural networks. We expect our 3D multifunctional MEA to open up opportunities for studies of neural circuits through precise, in vitro investigations of neural circuit dynamics with 3D brain models.
Recent studies have demonstrated the generation of midbrain‐like organoids (MOs) from human pluripotent stem cells. However, the low efficiency of MO generation and the relatively immature and ...heterogeneous structures of the MOs hinder the translation of these organoids from the bench to the clinic. Here we describe the robust generation of MOs with homogeneous distribution of midbrain dopaminergic (mDA) neurons. Our MOs contain not only mDA neurons but also other neuronal subtypes as well as functional glial cells, including astrocytes and oligodendrocytes. Furthermore, our MOs exhibit mDA neuron‐specific cell death upon treatment with 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine, indicating that MOs could be a proper human model system for studying the in vivo pathology of Parkinson's disease (PD). Our optimized conditions for producing homogeneous and mature MOs might provide an advanced patient‐specific platform for in vitro disease modeling as well as for drug screening for PD.
This study describes the novel strategy for the robust generation of homogeneous midbrain‐like organoids (MOs) using specific combination of dual SMAD inhibitors and in vitro WNT gradient. DAC3.0 MOs generated by our optimized protocol with in vivo‐like cellular composition are as structurally and functionally mature as the developing midbrain. DAC3.0 MOs with functional glial cells including astrocytes facilitate 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine‐based in vitro disease modeling of PD, suggesting its potential usefulness for an advanced patient‐specific platform for in vitro disease modeling as well as for drug screening for PD.
As nano- and micro-sized plastics accumulate in the environment and the food chain of animals, including humans, it is imperative to assess the effects of nanoplastics in living organisms in a ...systematic manner, especially because of their ability to adsorb potential toxicants such as pollutants, heavy metals, and organic macromolecules that coexist in the environment. Using the zebrafish embryo as an animal model, we investigated the bioaccumulation and in vivo toxicity of polystyrene (PS) nanoplastics individually or in combination with the Au ion. We showed that smaller PS nanoplastics readily penetrated the chorion and developing embryos and accumulated throughout the whole body, mostly in lipid-rich regions such as in yolk lipids. We also showed that PS nanoplastics induced only marginal effects on the survival, hatching rate, developmental abnormalities, and cell death of zebrafish embryos but that these effects were synergistically exacerbated by the Au ion in a dose- and size-dependent manner. Such exacerbation of toxicity was well correlated with the production of reactive oxygen species and the pro-inflammatory responses synergized by the presence of PS, supporting the combined toxicity of PS and Au ions. The synergistic effect of PS on toxicity appeared to relate to mitochondrial damage as determined by ultrastructural analysis. Taken together, the effects of PS nanoplastics were marginal but could be a trigger for exacerbating the toxicity induced by other toxicants such as metal ions.
The acetylcholinesterase inhibitors donepezil and rivastigmine have been used as therapeutic drugs for Alzheimer’s disease (AD), but their effects on LPS- and Aβ-induced neuroinflammatory responses ...and the underlying molecular pathways have not been studied in detail in vitro and in vivo. In the present study, we found that 10 or 50 μM donepezil significantly decreased the LPS-induced increases in the mRNA levels of a number of proinflammatory cytokines in BV2 microglial cells, whereas 50 μM rivastigmine significantly diminished only LPS-stimulated IL-6 mRNA levels. In subsequent experiments in primary astrocytes, donepezil suppressed only LPS-stimulated iNOS mRNA levels. To identify the molecular mechanisms by which donepezil regulates LPS-induced neuroinflammation, we examined whether donepezil alters LPS-stimulated proinflammatory responses by modulating LPS-induced downstream signaling and the NLRP3 inflammasome. Importantly, we found that donepezil suppressed LPS-induced AKT/MAPK signaling, the NLRP3 inflammasome, and transcription factor NF-kB/STAT3 phosphorylation to reduce neuroinflammatory responses. In LPS-treated wild-type mice, a model of neuroinflammatory disease, donepezil significantly attenuated LPS-induced microglial activation, microglial density/morphology, and proinflammatory cytokine COX-2 and IL-6 levels. In a mouse model of AD (5xFAD mice), donepezil significantly reduced Aβ-induced microglial and astrocytic activation, density, and morphology. Taken together, our findings indicate that donepezil significantly downregulates LPS- and Aβ-evoked neuroinflammatory responses in vitro and in vivo and may be a therapeutic agent for neuroinflammation-associated diseases such as AD.
Autophagy is a lysosomal degradative process which recycles cellular waste and eliminates potentially toxic damaged organelles and protein aggregates. The important cytoprotective functions of ...autophagy are demonstrated by the diverse pathogenic consequences that may stem from autophagy dysregulation in a growing number of neurodegenerative disorders. In many of the diseases associated with autophagy anomalies, it is the final stage of autophagy–lysosomal degradation that is disrupted. In several disorders, including Alzheimer's disease (AD), defective lysosomal acidification contributes to this proteolytic failure. The complex regulation of lysosomal pH makes this process vulnerable to disruption by many factors, and reliable lysosomal pH measurements have become increasingly important in investigations of disease mechanisms. Although various reagents for pH quantification have been developed over several decades, they are not all equally well suited for measuring the pH of lysosomes. Here, we evaluate the most commonly used pH probes for sensitivity and localisation, and identify LysoSensor yellow/blue‐dextran, among currently used probes, as having the optimal profile of properties for measuring lysosomal pH. In addition, we review evidence that lysosomal acidification is defective in AD and extend our original findings, of elevated lysosomal pH in presenilin 1 (PS1)‐deficient blastocysts and neurons, to additional cell models of PS1 and PS1/2 deficiency, to fibroblasts from AD patients with PS1 mutations, and to neurons in the PS/APP mouse model of AD.
Autophagy is a process to recycle cellular waste that relies on correct functioning of the lysosome. The complex regulation of lysosomal pH makes this process vulnerable to disruption by many factors and reliable lysosomal pH measurements are increasingly important in investigations of disease mechanisms. Here we evaluate the most commonly used pH probes and review evidence that lysosomal acidification is defective in Alzheimer's disease (AD), extending our original findings of elevated lysosomal pH in presenilin 1 knockout cells.
The triboelectric nanogenerator (TENG) is a new type of energy generator first demonstrated in 2012. TENGs have shown potential as power sources for electronic devices and as sensors for detecting ...mechanical and chemical stimuli. To date, studies on TENGs have focused primarily on optimizing the systems and circuit designs or exploring possible applications. Even though triboelectricity is highly related to the material properties, studies on materials and material designs have been relatively less investigated. This review article introduces recent progress in TENGs, by focusing on materials and material designs to improve the electrical output and sensing performance. This article discusses the current technological issues and the future challenges in materials for TENG.Nanotechnology: Materials for harvesting energy from motionThe development of materials for a technology that uses the movement of the human body to provide power has been reviewed by scientists in South Korea. A triboelectric nanogenerator converts mechanical energy into electricity by harnessing the fact that two surfaces rubbing against one another can become electrically charged. This is known as the triboelectric effect. One exciting use for these nanogenerators is in wearable electronics, where the motion of the body provides the power. Unyong Jeong and colleagues from Pohang University of Science and Technology have reviewed recent progress in material advances in the four main elements of a triboelectric nanogenerator: the charge-generating layer, the charge-trapping layer, the charge-collecting layer, and the charge-storage layer. These improvements all aim to increase the electrical output of such devices.
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