The purpose is to mitigate network congestion (NC) and high energy consumption (EC) in the traditional Internet of Things (IoT)-supported crop monitoring system (CMS). Firstly, the current work ...summarizes the status quo of IoT and edge computing (EC) technologies. Secondly, it constructs an intelligent multi-sensor-based real-time CMS. Consequently, an EC-based agricultural IoT (AIoT) architecture is proposed. Finally, the current work optimizes the task scheduling at the IoT edges using deep reinforcement learning (DRL) and proposes the DRL-optimized EC-AIoT-based CMS. Furthermore, the performance of the proposed DRL-optimized EC-AIoT-based CMS is verified through experiments. The results show that: (1) There is little difference between the data collected by the proposed CMS and the manual measurement, so the proposed CMS has a high data accuracy. (2) The performance of the DRL-optimized real-time scheduling model is better than the traditional methods in both scheduling time and data integrity. (3) Under the proposed EC-AIoT-based CMS, the server occupancy and queueing time are significantly lower than other algorithms. The purpose is to provide important technical support (TS) for improving the efficiency and quality of crop monitoring and agricultural green productivity (GP).
In view of the particularity of agricultural products and the shortcomings of existing agricultural products logistics distribution system, to reduce the cost of agricultural e-commerce logistics ...distribution and improve customer satisfaction, in this exploration, data mining technology in the field of artificial intelligence is used. Based on the analysis of the distribution efficiency of the current mainstream agricultural products logistics distribution mode, the logistics mode is optimized and improved, and the agricultural e-commerce common delivery mode is proposed. The logistics cost and customer satisfaction under the logistics mode are tested and analyzed through genetic algorithm and MATLAB software. The results show that the timely distribution rate of three modes of self-operated distribution, third-party logistics and common delivery decreases with the increase of orders; the timely distribution rate of common delivery mode fluctuates the most, followed by the third-party logistics mode; the timely distribution rate of self-operated distribution mode is the stablest, but it is relatively low. The calculation of genetic algorithm reveals that the optimized common delivery mode of agricultural products logistics has a significant effect on oil consumption, damage cost and other aspects. By implementing the common delivery mode, the oil consumption cost, the penalty cost, the refrigeration cost, and the damage cost are optimized by 26.7%, 31.7%, 30.3% and 19.6%, respectively. Moreover, 95% of customers are totally satisfied. In this exploration, the data mining technology is used to optimize the agricultural products distribution mode, which makes a beneficial exploration for solving the logistics bottleneck problem in the e-commerce environment, enriches the research of e-commerce logistics distribution system, and provides reference for promoting the development of agricultural products e-commerce logistics system and improving the efficiency of agricultural products logistics distribution.
Colorectal cancer (CRC) is one of the most common malignances worldwide. Chemoresistance remains a major issue in the field of CRC treatment. The present study aimed to investigate the potential role ...of polo-like kinase 2 (Plk2) in chemoresistance in CRC. The associations between Plk2 and clinicopathological factors, as well as chemotherapeutic benefit were analyzed with a publicly available CRC dataset. The correlation between Plk2 expression and chemosensitivity was further confirmed in CRC cells. Moreover, knockdown and exogenous overexpression experiments of Plk2 were carried out to uncover the potential role of Plk2 in regulating the chemoresistance of CRC cells. We found that the expression of Plk2 was significantly associated with proximally located tumors. In addition, it was found that high expression ofPlk2 was associated with deficient mismatch repair status, B-raf serine/threonine kinase proto-oncogeneand Kirsten rat sarcoma viral oncogene homolog mutations. By contrast, tumor protein 53 mutation was correlated with a low expression level of Plk2. A higher expression level of Plk2 significantly predicted a poorer outcome in patients with CRC. However, the prognostic significance was only observed in patients who received adjuvant chemotherapy. In CRC cells, higher levels of Plk2 were associated with increased resistance to chemotherapeutic agents. Knocking down the expression of Plk2 resulted in elevated cellular apoptosis induced by oxaliplatin. By contrast, exogenous overexpression of Plk2 exerted an anti-apoptotic effect and enhanced the resistance of CRC cells to chemotherapeutic agents. In conclusion, a high expression of Plk2 was associated with chemoresistant traits of CRC through inhibiting apoptosis. These results suggested that Plk2 may serve as a predictive marker for chemoresistance and a novel target in CRC treatment. Key words: colorectal cancer, polo-like kinase 2, outcome, adjuvant chemotherapy, chemoresistance, apoptosis
Purpose
This study aimed at exploring whether the time interval (TI) between prostate biopsy and surgery affect the outcomes of radical prostatectomy (RP).
Methods
A comprehensive search of the ...PubMed, Embase, and Cochrane Library databases was conducted to identify all eligible studies. After quality assessment and date extraction, a systematic review and meta-analysis was performed.
Results
A total of 9 studies with 8579 patients were included in our meta-analysis. Pooled data showed no significant differences between groups of TI ≤ 2 weeks and > 2 weeks in operative time, estimated blood loss, transfusion rate, bilateral nerve preservation, positively surgical margin, and complications. For comparison between TI ≤ 4 and > 4 weeks, shorter TI would be associated with significantly less estimated blood loss (
p
= 0.045) and lower rate of bilateral nerve preservation (
p
= 0.002). In addition, for TI ≤ 6 versus > 6 weeks, significantly less bilateral nerve preservation (
p
= 0.025) and more positive surgical margin (
p
= 0.020) could be found in the earlier surgery group. Sensitivity analysis indicated that TI had no impact on any outcomes of robot-assisted laparoscopic radical prostatectomy (RALP).
Conclusions
As shorter TI was associated with lower rate of bilateral nerve preservation and higher rate of positive surgical margin, it would be better to perform RP with a TI of 4 or 6 weeks after biopsy. While for RALP, shorter TI did not have any impact on outcomes of RALP, It is feasible and safe to perform RALP within 2, 4, or 6 weeks.
Background
This study intended to investigate the potential mechanism of microRNA‐200c‐3p (miR‐200c‐3p) and miR‐485‐5p in mediating the cisplatin (DDP) resistance in non–small cell lung cancer ...(NSCLC).
Methods
Quantitative real‐time polymerase chain reaction (qRT‐PCR) was applied to measure the expression of miR‐200c‐3p, miR‐485‐5p, and ribonucleotide reductase regulatory subunit M2 (RRM2) messenger RNA (mRNA). 3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay was used to analyze the DDP resistance and the proliferation of NSCLC cells. Colony formation assay was used to assess cell proliferation. Transwell assays were used to evaluate cell migration and invasion. The target relationship between RRM2 and miR‐200c‐3p or miR‐485‐5p was verified using dual‐luciferase reporter assay. The protein level of RRM2 was measured using Western blot assay. Animal experiments were conducted to analyze the roles of miR‐200c‐3p and miR‐485‐5p in the DDP resistance of xenograft tumors in vivo.
Results
MiR‐200c‐3p and miR‐485‐5p were both downregulated in DDP‐resistant NSCLC tissues and cell lines. Overexpressing miR‐200c‐3p or miR‐485‐5p suppressed the DDP resistance and malignant behaviors of NSCLC cells. MiR‐200c‐3p played a synergistic role with miR‐485‐5p in regulating the chemo‐resistance and biological behaviors NSCLC cells. RRM2 was confirmed as a target of miR‐200c‐3p and miR‐485‐5p. RRM2 silencing restrained the DDP resistance and progression of NSCLC. RRM2 overexpression partly reversed miR‐200c‐3p or miR‐485‐5p‐induced influences in NSCLC cells. The overexpression of miR‐200c‐3p or miR‐485‐5p aggravated DDP‐mediated suppressive effect on tumor growth in vivo.
Conclusion
MiR‐200c‐3p or miR‐485‐5p enhanced the DDP sensitivity and suppressed the malignant behaviors of NSCLC cells partly through targeting RRM2.
MiR‐200c‐3p and miR‐485‐5p were poorly expressed in DDP‐resistant NSCLC. RRM2 acted as a target of both of them, and miR‐200c‐3p and miR‐485‐5p sequestered RRM2 expression to regulate the proliferation, migration, invasion, and apoptosis of DDP‐resistant NSCLC cells via regulating the PI3K/AKT signaling pathway.
Mycoplasma pneumoniae (MP) can infect both the upper and lower respiratory tracts related diseases and a common cause of community-acquired pneumonia, mostly in young children and adolescents. ...Zingiber zerumbet (L.) belongs to the family of Zingiberaceae and it is a perennial and aromatic plant that cultivates in subtropical and tropical countries. This plant is traditionally found throughout Southeast Asia and the Pacific region, where it is commonly used in foods, and beverages purposes. Zingiber zerumbet is a valuable foundation of diverse classes of bioactive major compounds that fit to a varied diversity of chemical metabolites, including polyphenols, alkaloids and terpenes. The numerous studies of Z. zerumbet have shown the enormous pharmacological potential of this plant and its derived bioactive compounds in the treatment of various immune-related diseases like inflammation and other chronic diseases. Based on the previous scientific reports, there are no scientific investigations that claim the antipneumonial activity of the Z. zerumbet based silver nanoparticle. Therefore, the aim of the present study was designed and evaluated the anti-pneumonial potential of biosynthesized with Z. zerumbet based silver nanoparticles in mycoplasmal pneumonia in experimental rats.
Objective To investigate the role of transient receptor potential melatonin 2 （TRPM2） ion channels in microglia and astrocytes in temporal lobe epilepsy （TLE） -associated neuroinflammation. Methods ...Rats were randomly divided into the control group and epilepsy group. Seizure models were induced by lithium chloride-pilocarpine in the epilepsy group， and those in the control group were injected with the same dose of saline. The TLE rats were randomly divided into 7 subgroups according to the observation time after model establishment： acute phase （3 h group， 6 h group， 1 d group， 2 d group）， latent phase （14 d group） and chronic phase （30 d group， 90 d group） subgroups （n = 5）. The expression level of TRPM2 in the hippocampus of TLE rats at different stages were detected， and the cellular localization of TRPM2 in the brain of TLE rats was investigated. BV2 and C8 cell lines were induced by hydrogen peroxide （H2O2）. The levels of IL1-β， IL-6 and TNF-α released by BV2 and C8 cells were observed by ELISA. The leve
Background
Non‐small‐cell lung cancer (NSCLC) is one of the most common malignant tumors on earth. Circular RNAs have been disclosed to be vital regulators in the chemoresistance and development of ...diverse cancers, including NSCLC. Here, we attempted to explore the function of circ_0011292 in paclitaxel (PTX)‐resistant NSCLC cells.
Methods
Quantitative real‐time polymerase chain reaction or western blot was performed to detect the expression of circ_0011292, microRNA‐433‐3p (miR‐433‐3p), and checkpoint kinase 1 (CHEK1). Ribonuclease R (RNase R) assay was performed to assess the stability of circ_0011292. Cell Counting Kit‐8 assay was conducted to evaluate the half maximal inhibitory concentration of PTX and cell viability. Cell proliferation was monitored by Edu incorporation and colony formation assays. Cell cycle and apoptosis were detected by flow cytometry. Transwell assay was implemented to assess cell migration and invasion. Western blot assay was utilized to determine the protein levels. Dual‐luciferase reporter assay was carried out to verify the targeted interaction between miR‐433‐3p and circ_0011292 or CHEK1. Xenograft tumor model was constructed for determining the effect of circ_0011292 in NSCLC growth in vivo.
Results
Circ_0011292 was upregulated in PTX‐resistant NSCLC tissues and cells. Circ_0011292 or CHEK1 knockdown enhanced PTX sensitivity and cell apoptosis, and repressed cell proliferation, migration, and invasion in PTX‐resistant NSCLC cells. Mechanistically, circ_0011292 was a sponge of miR‐433‐3p and miR‐433‐3p directly targeted CHEK1. Meanwhile, silencing miR‐433‐3p or overexpressing CHEK1 respectively abrogated the impacts of circ_0011292 deletion or miR‐433‐3p introduction on PTX resistance and cell progression in PTX‐resistant NSCLC cells in vitro. Moreover, circ_0011292 could positively modulate CHEK1 expression through sponging miR‐433‐3p. In addition, circ_0011292 knockdown retarded tumor growth of NSCLC in vivo.
Conclusion
Circ_0011292 could accelerate PTX resistance and cell malignant progression of NSCLC cells partially through the regulation of circ_0011292/miR‐433‐3p/CHEK1 axis.
Circ_0011292/miR‐433‐3p/CHEK1 axis promotes PTX resistance, cell proliferation, migration and invasion and inhibits apoptosis in PTX‐resistant non‐small cell lung cancer cells.
Tubulin plays an essential role in cortical development, and
encodes a major neuronal
-tubulin. Neonatal mutations in
are associated with severe brain malformations, and approximately 70% of patients ...with reported cases of
mutations exhibit lissencephaly. We report the case of a 1-year-old boy with the
nascent mutation c.1204C >T, p.Arg402Cys, resulting in lissencephaly, developmental delay, and seizures, with a brain MRI showing normal cortical formation in the bilateral frontal lobes, smooth temporo-parieto-occipital gyri and shallow sulcus. This case has not been described in any previous report; thus, the present case provides new insights into the broad disease phenotype and diagnosis associated with
mutations. In addition, we have summarized the gene mutation sites, neuroradiological findings, and clinical details of cases previously described in the literature and discussed the differences that exist between individual cases of
mutations through a longitudinal comparative analysis of similar cases. The complexity of the disease is revealed, and the importance of confirming the genetic diagnosis from the beginning of the disease is emphasized, which can effectively shorten the diagnostic delay and help clinicians provide genetic and therapeutic counseling.
Epilepsy, one of the most common neurological disorders, is characterized by spontaneous recurrent seizures. Temporal lobe epilepsy (TLE) is one of the most common medically intractable seizure ...disorders. Traf2-and NcK-interacting kinase (TNIK) has recently attracted attention as a critical modulation target of many neurological and psychiatric disorders, but its role in epilepsy remains unclear. In this study, we hypothesized the involvement of TNIK in epilepsy and investigated TNIK expression in patients with intractable TLE and in a pilocarpine-induced rat model of epilepsy by western blotting, immunofluorescence, and immunohistochemistry. A pentylenetetrazole (PTZ)-induced epilepsy rat model was used to determine the effect of the TNIK inhibitor NCB-0846 on behavioral manifestations of epilepsy. Coimmunoprecipitation (Co-IP)/mass spectrometry (MS) was used to identify the potential mechanism. Through Co-IP, we detected and confirmed the main potential TNIK interactors. Subcellular fractionation was used to establish the effect of NCB-0846 on the expression of the main interactors in postsynaptic density (PSD) fractions. We found that TNIK was primarily located in neurons and decreased significantly in epilepsy model rats and TLE patients compared with controls. NCB-0846 delayed kindling progression and decreased seizure severity. Co-IP/MS identified 63 candidate TNIK interactors in rat hippocampi, notably CaMKII. Co-IP showed that TNIK might correlate with endogenous GRIA1, SYN2, PSD-95, CaMKIV, GABRG1, and GABRG2. In addition, the significant decrease in GRIA1 in hippocampal total lysate and PSDs after NCB-0846 treatment might help modify the progression of PTZ kindling. Our results suggest that TNIK contributes to epileptic pathology and is a potential antiepileptic drug target.
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