To investigate the correlation between the change of liver enzymes and the severity of acute graft-versus-host disease (aGVHD).
The liver enzyme and severity of aGVHD in 82 patients were analyzed ...retrospectively.
Among the 82 aGVHD patients, 7 developed grade I (18.3%), 47 grade II (57.3%), 13 grade III (15.9%) and 7 grade IV (8.5%) aGVHD. The elevation of ALT, AST and ALP was found in 49 (59.8%), 36(43.9%) and 8(9.8%)patients respectively. Among these patients, 34 (69.4%) were ALT > 40 U/L, 18 (50%) AST > 40 U/L and 4 (50%) ALP > 132 U/L before the onset of aGVHD. The elevation of ALT and AST was more common and the peak value was significantly higher in patients with grade III-IV aGVHD than with grade I-II aGVHD, but those of ALP showed no difference between the two groups. Compared with the patients without ALT elevation, the proportion of severe aGVHD was higher in patients with ALT elevation.
The alteration of liver enzymology is common in aGVHD and its level is correlated with the severity of aGVHD. Liv
To evaluate preliminarily the significance of dynamic detection of Wilms' tumor gene (WT1) expression level on monitoring minimal residual disease (MRD) and predicting clinical relapse in patients of ...malignancy following allogeneic hematopoietic stem cell transplantation (allo-HSCT).
WT1 expression level was measured with real-time quantitative reverse transcription polymerase chain reaction (RQ-RT-PCR) method on 102 bone marrow specimens from 31 patients following allo-HSCT. WT1 expression level was determined as the ratio of WT1 mRNA to ABL mRNA times 100%.
The levels of WT1 expression showed significant difference between the relapsed group and the non-relapsed group (P < 0.01), with 0.80% and 0.17% as their median expression level respectively. After dynamic measuring WT1 expression level on patients in the relapsed group, the level was found to increase significantly as compared with those during or before the clinical hematological relapse, both being > 1.0%. The level at the time of relapse was signifi
We introduce a twice differentiable augmented Lagrangian for nonlinear optimization with general inequality constraints and show that a strict local minimizer of the original problem is an ...approximate strict local solution of the augmented Lagrangian. A novel augmented Lagrangian method of multipliers (ALM) is then presented. Our method is originated from a generalization of the Hetenes-Powell augmented Lagrangian, and is a combination of the augmented Lagrangian and the interior-point technique. It shares a similar algorithmic framework with existing ALMs for optimization with inequality constraints, but it can use the second derivatives and does not depend on projections on the set of inequality constraints. In each iteration, our method solves a twice continuously differentiable unconstrained optimization subproblem on primal variables. The dual iterates, penalty and smoothing parameters are updated adaptively. The global and local convergence are analyzed. Without assuming any constraint qualification, it is proved that the proposed method has strong global convergence. The method may converge to either a Kurash-Kuhn-Tucker (KKT) point or a singular stationary point when the converging point is a minimizer. It may also converge to an infeasible stationary point of nonlinear program when the problem is infeasible. Furthermore, our method is capable of rapidly detecting the possible infeasibility of the solved problem. Under suitable conditions, it is locally linearly convergent to the KKT point, which is consistent with ALMs for optimization with equality constraints. The preliminary numerical experiments on some small benchmark test problems demonstrate our theoretical results.
Coherent preemptive therapy criterion was applied in various kinds of allogenic hematopoietic stem-cell transplant (allo-HSCT) recipients with cytomegalovirus (CMV) infection in order to evaluate its ...clinical value in various kinds of HSCT.
A total of 318 allo-HSCT patients were monitored for CMV infection. Real-time quantitative polymerase chain reaction (RQ-PCR) was performed to serially monitor CMV viremia after transplantation. There were 136 patients infected with CMV including 31 of HLA-matched sibling, 88 of HLA-mismatched related and 17 of unrelated donor HSCT. Coherent preemptive therapy criterion was employed. The clearance rate of DNAemia, incidence of CMV disease and overall survival rate were compared among the three types of HSCT.
All the 136 patients with CMV DNAemia were treated with ganciclovir, foscarnet and valganciclovir, respectively. The period of treatment and the clearance rate of DNAemia were comparable among the HLA-matched, HLA-mismatched related and unrelated donor HSCT groups (96.
For high density polyethylene pipe connection，welding technology is the key of the high density engineering plastic pressure pipe safety. And the temperature distribution in the welding process has a ...very important influence on the welding quality. Polyethylene pipe weld joints of one dimensional unsteady overall heat transfer model is established by MARC software and simulates temperature field and stress field distribution of the welding process，and the thermocouple temperature automatic acquisition system of welding temperature field changes were detected，and compared by simulation and experiment .The results show that，at the end of the heating，the temperature of the pipe does not reach the maximum，but reached the maximum at 300 s，which indicates that the latent heat of phase change in the process of pressure welding. In the process of pressure welding， the axial stress of the pipe is gradually changed from tensile stress to compressive stress.
Background In haploidentical hematopoietic stem cell transplantation （HSCT）, the duration of graft-versus-host disease （GVHD） prophylaxis after modified donor lymphocyte infusion （DLI） was the only ...risk factor of DLI-associated grades 3-4 acute GVHD. However, the successful application of modified DLI depended not only on the reduction of severe GVHD, but also on the preservation of graft-versus-leukemia （GVL） effect. Therefore, this study was performed to compare the impact of prophylaxis for 6-8 weeks and prophylaxis for 〈6 weeks on GVL effect after modified DLI in haploidentical HSCT. Methods A total of 103 consecutive patients developing hematological relapse or minimal residual disease （MRD）-positive status after haploidentical HSCT and receiving modified DLI were investigated retrospectively. Fifty-two patients received prophylaxis for 6-8 weeks after modified DLI; the remaining 51 patients received prophylaxis for 〈6 weeks. Results First, compared with prophylaxis for 〈6 weeks, prophylaxis for 6-8 weeks reduced incidence of relapse in total patients （26.6% vs. 69.0%, P 〈0.001）. Besides, prophylaxis for 6-8 weeks also reduced incidence of relapse in 54 patients developing hematological relapse post-transplant （P=0.018） and in 49 patients developing MRD-positive status post-transplant （P 〈0.001）. Second, prophylaxis for 6-8 weeks reduced incidence of acute GVHD （P 〈0.05）, reduced the therapeutic application of immunosuppressive agents （P=0.019）, but increased the incidence of chronic GVHD （P〈0.05）. Third, prophylaxis for 6-8 weeks improved overall survival and disease-free survival in total patients, as well as in patients developing hematological relapse post-transplant and in patients developing MRD-positive status post-transplant （P 〈0.05）. Conclusions In haploidentical HSCT, prophylaxis for 6-8 weeks after modified DLI does not reduce GVL effect, but reduces the incidence of DLI-associated acute GVHD compared with prophylaxis for 〈6 weeks. This strategy will probably improve the safety and efficacy of modified DLI further.
Three new cycloartane triterpenoids (1–3) and two known compounds (4, 5) were isolated from the whole plant of Beesia calthaefolia. Their structures were elucidated by 1D and 2D NMR, HRESIMS and ...optical rotation spectral data. All isolates were investigated for their inhibitory effects on the classical pathway of the complement system. Among them, compound 4 showed stronger inhibitory activity (IC50 136.7μM) than positive control (Rosmarinic acid, IC50 181.8μM) while compounds 2 and 3 were moderately active with IC50 value of 206μM and 200.9μM. Chemical compound studied in this article: Rosmarinic acid (PubChem CID: 5281792).
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