Neighborhood micro-renewal (NMIR) plays a critical role in revitalizing old neighborhoods and urban sustainability. Public participation has attracted widespread concern to satisfy the multiple ...demands for social governance in China. Prior studies have underlined the promotion of communal participation, whereas collective characteristics and individual intentions of public behavior have been largely neglected. This study investigates determinants of residents' participation intention and behavior in a framework that incorporates the theory of planned behavior (TPB) and the expectation-confirmation model (ECM). Based on structural equation modeling (SEM), the empirical analysis was conducted with questionnaires completed by 443 residents who dwell in a neighborhood undergoing micro-renewal. The results indicated that residents' participatory intention and behavior were predicted by subjective norms, behavioral attitudes, and perceived behavioral control. In addition, perceived usefulness positively affected behavioral intention, whereas the impact of satisfaction was not significant. Furthermore, community attachment could positively moderate the effect of intention on behavior. Accordingly, several targeted managerial implications regarding public participation in NMIR are proposed. This study extends the TPB into the practice of public involvement on a neighborhood scale and provides insightful ideas to promote residents’ participation awareness.
•An extended theory of planned behavior is adopted to uncover residents' participatory intention for neighborhood renewal.•These residents with high community attachment are more likely to participate in the neighborhood renewal.•Subjective norm is the uppermost predictor, indicating the critical role of external influence on participation intention.•The positive effect of satisfaction on behavioral intention is not supported significantly.
Urban renewal involves a wide range of stakeholders with diverse expectations and interests. Conflicts in urban renewal projects arise from intricate relationships among multiple stakeholders, ...hindering the urban renewal process. With a large amount of current literature examining the barriers, difficulties, and solutions in urban regeneration, a critical review is required to holistically summarize these main concerns and challenges from the stakeholder perspective. Based on 347 journal papers collected from the Web of Science core database, this study investigates the development, trajectory, and tendency of prior studies through a bibliometric analysis. Then, a critical review is documented with eight critical barriers in the economic and social aspects from the stakeholder perspective. To address these issues, this study proposes a strategic framework for value creation, collaborative governance, and benefit sharing. Accordingly, future research agendas are also presented. This study could provide researchers with a systematic understanding of the critical barriers and potential strategies in urban regeneration fields.
Abstract
The reasonable layout of green infrastructure is conducive to the low-carbon, livable and high-quality sustainable development of cities. The framework of spatio-temporal evolution ...characteristics and prediction analysis of Urban Green Infrastructure (UGI) was constructed by integrating morphological spatial pattern analysis (MSPA) and CA-Markov in the study. We analyzed the spatio-temporal evolution characteristics of UGI in Beijing from 1990 to 2019, predicted its future change trend in 2030, and put forward the optimization scheme for the ecological network of UGI. The area change of UGI presented a "V" shape from 1990 to 2019 in Beijing, and the turning point was around 2009. Its spatial distribution revealed a significant heterogeneity. The comprehensive change rate index showed a "rising and then falling" trend from 1990 to 2019. Core with an area of over 1000 km
2
had inclined "C" shape, connecting the north, west and south of the study area. Among the three prediction scenarios for 2030, the area of UGI under the ecological conservation priority scenario is the largest, accounting for 86.35% of the total area. The area of UGI under the economic development priority scenario is the smallest, accounting for 76.85%. The optimization of zoning and road network are effective measures to improve the connectivity of UGI in Beijing. This study is beneficial to extend the research ideas of UGI and promote sustainable urban development.
The matrix (M) protein of Newcastle disease virus (NDV) contains large numbers of unevenly distributed basic residues, but the precise function of most basic residues in the M protein remains ...enigmatic. We previously demonstrated that the C-terminus (aa 264-313) of M protein interacted with the extra-terminal (ET) domain of chicken bromodomain-containing protein 2 (chBRD2), which promoted NDV replication by downregulating chBRD2 expression and facilitating viral RNA synthesis and transcription. However, the key amino acid sites determining M's interaction with chBRD2/ET and their roles in the replication and pathogenicity of NDV are not known. In this study, three basic residues-R283, R286, and K288-in the NDV M protein were verified to be responsible for its interaction with chBRD2/ET. In addition, mutation of these basic residues (R283A/R286A/K288A) in the M protein changed its electrostatic pattern and abrogated the decreased expression of endogenic chBRD2. Moreover, a recombinant virus harboring these mutations resulted in a pathotype change of NDV and attenuated viral replication and pathogenicity in chickens due to the decreased viral RNA synthesis and transcription. Our findings therefore provide a better understanding of the crucial biological functions of M's basic residues and also aid in understanding the poorly understood pathogenesis of NDV.
Within the framework of the configuration–interaction shell model, the present work applies three effective interactions to investigate the effects of the cross-shell excitation on F and Ne isotopes ...around N = 20, which are significantly proton–neutron asymmetric, and have different properties compared with the proton–neutron symmetric nuclei. It is shown that cross-shell excitation is necessary in order to reproduce separation energies, neutron drip lines, and low-energy levels of these isotopes. Furthermore, the cross-shell excitation of (0–5)ħω is suggested to be important in the description of 29F and 30Ne. However, the three interactions are insufficient in describing the bound structure of 29,31Ne, and provide inconsistent shell structures and evolutions in the target nuclei. Their cross-shell interactions are suggested to be improved.
The carbon-intensive economy has dramatically caused global climate changes and profoundly impacted humankind. As one of the largest energy consumers, carbon emissions in the construction industry ...(CECI) play a crucial role in achieving the carbon neutrality goal. Government behaviors could significantly affect CECI. However, few studies have comprehensively reviewed existing literature regarding the effect of government instruments on triggering carbon reduction. A total of 1,082 papers about CECI from 51 countries/regions were retrieved in this study, while 296 relevant articles on the government behaviors in CECI were collected to conduct further analysis. Based on the bibliometric analysis with CiteSpace, the co-occurrence networks of countries/regions, institutions, keywords and cluster analysis are applied to illustrate the characteristics of previous studies. Furthermore, a research framework has been formulated to review the impact of government behaviors on CECI during the life cycle of buildings. The result indicated that government behaviors could affect CECI through three stages, i.e., material production, construction and operation, which is considered the transmission path of government behaviors towards CECI. Moreover, the findings revealed that government behaviors present the most significant impact on CECI in the following sectors: 1) the green supply chain management and waste recycling in the material production stage; 2) the green building decisions and the adoption of off-site construction in the construction stage; 3) energy conservation behaviors and green retrofit decisions in the operation stage. Finally, this study discusses prior study gaps and provides potential directions for future research.
S100A16 is a member of the S100 calcium-binding protein family. It is overexpressed in many types of tumors and associated with proliferation, migration, and invasion; however, its function in human ...prostate cancer is unresolved. Our objective was to determine its effects and the underlying pathways of S100A16 in prostate cancer tissues and cells. We measured S100A16 expression by quantitative real-time polymerase and Western blotting in eight matched prostate cancer and adjacent normal tissues, and in three prostate cancer cell lines, DU-145, LNCaP, and PC-3, compared to a normal prostate epithelial cell line PrEC. DU-145 cells stably overexpressing S100A16 and PC-3 cells with S100A16 knockdown were established by transfection with S100A16 overexpression plasmid or shRNAs. Invasion, migration, and proliferation were analyzed by transwell assay, wound healing, and colony formation assays, respectively. Western blotting and invasion assays were performed to determine expressions and activation of AKT, ERK, p21, and p27. S100A16 was significantly overexpressed in both prostate cancer tissues and cells lines compared to normal controls (
P
< 0.05). Overexpression of S100A16 significantly promoted invasion, migration, and proliferation in prostate cancer cells in vitro, whereas silencing S100A16 showed the converse effects (
P
< 0.05). Furthermore, overexpression of S100A16 activated cell signaling proteins AKT and ERK and downregulated tumor suppressors p21 and p27. Specific inhibitors, LY294002 and PD98059, suppressed activation of AKT and ERK, which attenuated DU-145 cell clone formation and invasion induced by S100A16 overexpression. S100A16 may promote human prostate cancer progression via signaling pathways involving AKT, ERK, p21, and p27 downstream effectors. Our findings suggest that S100A16 may serve as a novel therapeutic or diagnostic target in human prostate cancer.
Up to now, the nuclear shell model is rarely used in the nuclear data study because of several reasons. First, medium and heavy mass nuclei far from the shell-model cores, normally doubly magic ...nuclei, require a huge amount of calculation resource even in a limited shell-model space. Second, large deformation is difficult to be described in the limited model space, which is based on spherical symmetry. Third, high precision evaluation of nuclear structure data challenges the ability of the shell model. Even so, it is worth starting preliminary nuclear data investigations based on the shell model. With the present computational ability, it is possible to investigate 1000 or more nuclei in the framework of the shell model, which should be helpful for nuclear data study. In the present work, some recent shell-model investigations are briefly introduced. Based on these works, a simple nuclear force is suggested to be used in the systematic nuclear structure data study. The south-west region of
132
Sn is taken as an example to show the ability of such a simple nuclear force.
Soybean protein‐derived peptides (SBP) are a rich source of various bioactive peptides with multiple health benefits. However, the prospective effects of SBP on human cells are still unclear. ...Therefore, this article investigated the effects of small molecular weight SBP on MG132‐induced apoptosis in RAW264.7 cells. SBP inhibited MG132‐induced apoptosis of RAW264.7 cells in a dose‐dependent manner by flow cytometry. To further study its molecular mechanisms, Western blot analysis demonstrated that SBP could activate the PI3K‐AKT pathway by increasing the phosphorylation of PI3K and AKT and inhibiting apoptosis pathway by downregulating the expressions of pro‐apoptotic proteins of Bim, Bax, Fas, and Fasl and promoting the expressions of anti‐apoptotic proteins of Bcl‐xL and Bcl‐2. These results indicated the protective effect of SBP on MG132‐induced apoptosis in RAW264.7 cells.
Soybean peptides display anti‐apoptosis effect in RAW264.7 cells through the activation of PI3K‐AKT pathway or indirect binding to Fas and FasL receptor.
The aim of this study is to determine the effects of E2 on metabolic syndrome and the molecular mechanisms involving S100A16. Ovariectomized (OVX) rat models and mouse embryonic fibroblasts cell ...models were used. E2 loss in OVX rats induced body weight gain and central abdominal fat accumulation, which were ameliorated by E2 treatment under chow and high-fat diet (HFD) conditions. E2 decreased the expression of the adipocyte marker genes PPARγ, aP2, C/EBPα, and S100A16. E2 inhibited adipogenesis. Overexpression of S100A16 reversed the E2-induced adipogenesis effect. A luciferase assay showed that E2 inhibited the expression of S100A16. E2 treatment decreased body weight gain and central abdominal fat accumulation under both chow and HFD conditions. Also, E2 suppressed adipogenesis by inhibiting S100A16 expression.
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