OBJECTIVEThe objective was to study the anxiolytic effect of minocycline in resident-intruder social conflict in submissive hamsters post resident intrusion model using open field test (OFT) and ...elevated plus maze (EPM) and serum cortisol levels. MATERIALS AND METHODSFifty-two singly housed male Syrian hamsters were used, post standardization of an animal model. Resident intrusion was done (5 min), in which smaller hamsters were placed in the cage of larger hamster, and the behavior of smaller hamster was noted. Eight submissive hamsters per group (disease control, lorazepam group as a positive control, and the test drug was minocycline) were used, and the drug was administered immediately post resident intrusion, intraperitoneally. Behavioral tests, namely OFT and EPM, were done followed by retro-orbital blood collection for serum cortisol estimation. The level of significance was set at P < 0.05. RESULTSThe minocycline group showed a statistically significant decrease in serum cortisol levels compared to the disease control group. Among all the variables pertaining to both the behavioral tests, namely EPM and OFT, the results indicated an anxiolytic effect, which was statistically significant compared to the disease control group. CONCLUSIONAs per the biochemical test using serum cortisol levels and behavioral tests in the form of EPM and OFT, the study concluded that the anxiolytic effect of minocycline is at least comparable to the positive control, lorazepam.
The gut microbiome has varied impact on the wellbeing of humans. It is influenced by different factors such as age, dietary habits, socio-economic status, geographic location, and genetic makeup of ...individuals. For devising microbiome-based therapies, it is crucial to identify population specific features of the gut microbiome. Indian population is one of the most ethnically, culturally, and geographically diverse, but the gut microbiome features remain largely unknown. The present study describes gut microbial communities of healthy Indian subjects and compares it with the microbiota from other populations. Based on large differences in alpha diversity indices, abundance of 11 bacterial phyla and individual specific OTUs, we report inter-individual variations in gut microbial communities of these subjects. While the gut microbiome of Indians is different from that of Americans, it shared high similarity to individuals from the Indian subcontinent i.e., Bangladeshi. Distinctive feature of Indian gut microbiota is the predominance of genus Prevotella and Megasphaera. Further, when compared with other non-human primates, it appears that Indians share more OTUs with omnivorous mammals. Our metagenomic imputation indicates higher potential for glycan biosynthesis and xenobiotic metabolism in these subjects. Our study indicates urgent need of identification of population specific microbiome biomarkers of Indian subpopulations to have more holistic view of the Indian gut microbiome and its health implications.
Two Gram-stain positive, endospore forming, non-motile, rod shaped bacterial strains SN6
T
and SN6b were isolated from scats of a mildly venomous vine snake (
Ahaetulla nasuta
). Strains were ...phenotypically resistant to multiple antibiotics of four different classes i.e. aminoglycosides, β-lactams, fluoroquinolones and sulphonamides. Cells of both the strains were catalase positive and oxidase negative. Phylogenetic analysis based on 16S rRNA gene sequence analysis of these two strains showed closest similarity (99.2% and 99.3%) with
Savagea faecisuis
Con12
T
, the only species of the genus
Savagea
and ≤ 94.9% with the species of other closest genera of the family
Planococcaceae
. The 16S rRNA gene sequence similarity (99%), DNA-DNA relatedness (95%) and similar phenotypic characteristics between the strains SN6
T
and SN6b revealed their phylogenetic affiliation to the same species. Hence, strain SN6b is an additional strain of the type strain SN6
T
. DNA-DNA relatedness of strain SN6
T
with
S. faecisuis
Con12
T
was 32.8%. Predominant fatty acids were iso-C
15:0
(32.0%), iso-C
16:1
ω11c (19.2%) and iso-C
17:1
ω10c (12.1%). MK-6 (100%) was the only respiratory quinone of strain SN6
T
. Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine were the major polar lipids. Cell wall peptidoglycan was A4α; L-Lys-Gly-D-Glu type. The DNA G + C content (mol%) of SN6
T
was 40.8. Whole genome sequence of SN6
T
consisted of 26,37,389 base pairs in length with 2667 annotated genes, out of which 1021 corresponds to hypothetical proteins and 1646 with functional assignments including antibiotic resistance, multidrug resistance efflux pumps, invasion and virulence factors. Comparative polyphasic study of the strains SN6
T
, SN6b and
S. faecisuis
Con12
T
elucidated the differentiating characteristics which led to describing strain SN6
T
and SN6b as a novel species of the genus
Savagea
for which the name
Savagea serpentis
sp. nov is proposed. The type strain of
Savagea serpentis
is SN6
T
(= KCTC 33546
T
= CCUG 6786
T
).
Bank Loan approval is a very important process for banking organizations. The system approved or reject the loan applications. Recovery of loan is a major contributing parameter in the financial ...statements of a bank. It is very difficult to predict the possibility of repayment of loan by the customer. In recent years many researchers worked on loan approval prediction systems. In the System Machine Learning (ML) techniques are very useful in predicting outcomes for large amount of data. In this paper two machine learning algorithms, Support Vector Machine (SVM) and Random Forest (RF) are applied to predict the loan approval of customers.
The mechanical properties and microstructure of heat treated 6082 wrought aluminum alloy was studied. The aim of this work was to study the merits and demerits of natural aging and artificial aging ...on material properties. The precipitation hardening of aluminum alloy usually undergoes a thermal treatment consisting of heat treatment, quenching and aging. Experiments were carried out for different natural aging time (10 min, 30 min, 60 min and 120 min) and at different artificial aging temperatures (150 °C-210 °C at an interval of 10°C). The results were analyzed with optical microscope, scanning electron mic roscope (SEM), XRD and transmission electron microscope (TEM). The results showed that the type and distribution of precipitates significantly affect the mechanical properties. Natural aging affect the formation of metastable (Mg 5 Si 6 ) precipitates and stable (Mg 2 Si) precipitates, Peak hardness (strength) is achieved by metastable precipitates. As the natural aging time increases sides for the nucleation of precipitated decreases, hence the strength decreases.
PDF
