VCO (virgin coconut oil) has benefits for skin health such as softening the skin, maintaining skin health, and protecting the skin from radiation and free radicals. VCO as one of the raw materials ...for making cream preparations can affect its physical stability. This study aimed to analyze the effect of VCO on the physical stability of sweet potato leaf extract creams and antibacterial activity test against Staphylococcus aureus ATCC 25923. The creams were made with five formulas namely base, extract cream 2%, and VCO 1%, 3%, 5% added to the extract cream 2% respectively. Physical stability test included storage for six weeks at room temperature. Stability parameters were organoleptic observation, homogeneity, pH, spreadability, adhesivity, and cream type. Statistical analysis was carried out with the Anova and Kruskal Wallis tests. The organoleptic test results of the cream extract showed a distinctive odor, green color, with semisolid and homogeneous texture. Increasing the concentration of VCO will increase the spreadability of extract cream but did not affect pH and adhesion. The conclusion of this study was that extract cream 2% without the addition of VCO was the most stable cream formulation. Besides that, all the cream formulas did not show antibacterial activity against Staphylococcus aureus ATCC 25923.
Akar kuning (Fibraurea chloroleuca Miers) dengan senyawa metabolit sekunder yang terkandung di dalamnya merupakan salah satu tanaman yang berpotensi sebagai tanaman obat tradisional untuk mengobati ...berbagai penyakit. Tujuan penelitian ini adalah menentukan kadar alkaloid total ekstrak akar kuning dengan perbedaan konsentrasi pelarut etanol yaitu 50%, 70%, dan 96%. Proses ekstraksi menggunakan metode maserasi dan ekstrak diuapkan dengan rotary evaporator pada suhu 40oC. Identifikasi alkaloid pada ekstrak menggunakan pereaksi Wagner, Mayer dan Dragendorff dan kadar alkaloid total dilakukan dengan metode spektrofotometri UV-Vis. Hasil penelitian menunjukkan identifikasi alkaloid dengan semua pereaksi dinyatakan positif mengandung alkaloid pada ekstrak. Hasil kadar alkaloid total ekstrak akar kuning dengan pelarut etanol 50% sebesar 0,6939 ± 0,2439%, dengan pelarut etanol 70% sebesar 0,6607 ± 0,2117% dan pelarut etanol 96% sebesar 0,7826 ± 0,3004%. Berdasarkan hasil penelitian, ekstrak akar kuning dengan konsentrasi pelarut etanol 96% menunjukkan kadar alkaloid yang tertinggi.
Escherichia coli is a bacteria belonging to the family Enterobacteriaceae. Escherichia coli is a harmful gram bacterium living in the digestive tract. These bacteria also live in water and soil, ...commonly referred to as coliform. This research aims to analyze the plasmid profile of Escherichia coli bacteria and its resistance to some antibiotics. To explore the plasmid profiles of Escherichia coli in a recent research electrophoresis gel, the antibiotic used in its resistance tests is the Amoxicillin, Tetracycline antibiotic, Ciprofloxacin, Sulfamethoxazole, and Streptomycin antibiotic. The electrophoresis results in a difference in the number of plasmid profiles with 1 to 2 ribbons of plasmid DNA different sizes from ˜1500 bp to ˜2300 bp. Antibiotic resistance tests have occurred in Amoxicillin, Ciprofloxacin, and some resistance samples to the Streptomycin antibiotic.
Akar kuning (Fibraurea chloroleuca Miers) merupakan salah satu tanaman yang mempunyai banyak manfaat dalam pengobatan dengan kandungan metabolit sekunder berupa alkaloid, flavonoid, saponin, tanin, ...steroid dan terpenoid. Tujuan penelitian ini untuk mengetahui jenis dan kadar saponin ekstrak akar kuning. Proses ekstraksi telah dilakukan melalui metode refluks dengan pelarut metanol. Analisis jenis saponin menggunakan pereaksi LB (Liebermann-Burchard) dan kadarnya ditentukan dengan metode gravimetri. Hasil penelitian menunjukkan jenis saponin ekstrak akar kuning adalah saponin triterpenoid dengan kadar sebesar 4,51±0,2805%.
Akar kuning (Fibraurea chloroleuca Miers) merupakan salah satu tanaman yang mempunyai banyak manfaat dalam pengobatan dengan kandungan metabolit sekunder berupa alkaloid, flavonoid, saponin, tanin, ...steroid dan terpenoid. Tujuan penelitian ini untuk mengetahui jenis dan kadar saponin ekstrak akar kuning. Proses ekstraksi telah dilakukan melalui metode refluks dengan pelarut metanol. Analisis jenis saponin menggunakan pereaksi LB (Liebermann-Burchard) dan kadarnya ditentukan dengan metode gravimetri. Hasil penelitian menunjukkan jenis saponin ekstrak akar kuning adalah saponin triterpenoid dengan kadar sebesar 4,51±0,2805%.
Shallots are natural ingredients that are used as food enhancers for food and traditional medicines. This study aimed to analyze the specific and non-specific parameters of shallot (Allium cepa L.) ...Simplicia. Analysis of simplicia specific parameters in the form of material identity, organoleptic, macroscopic, and microscopic examination, levels of water-soluble extracts and ethanol soluble extracts while non-specifically in the form of a percentage of drying loss, water content, total ash, acid insoluble ash, and water-insoluble ash. The results of the research on specific parameters gave a yellowish-green simplicia color, a distinctive odor, in the form of powder, macroscopic examination of leeks elongated cylindrical, small round and hollow like a pipe, blunt leaf base, pointed leaf tip, green color, microscopic examination contained cover cells, gaps and epidermal cells, the levels for water-soluble extracts were 8.81±2.12%, the levels of ethanol-soluble compounds were 5.64±2.91%. The results of the study of non-specific parameters, the percentage of drying shrinkage was 0.89±0.57%, water content was 0.89±0.34%, total ash was 12.97±0.34%, acid-insoluble ash was 6.44±2.12%, and the water-insoluble ash content was 8.03±0.84%.
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