Entamoeba histolytica is an intestinal parasite that causes dysentery and amebic liver abscess. E. histolytica has the capability to invade host tissue by union of virulence factor Gal/GalNAc lectin; ...this molecule induces an adherence-inhibitory antibody response as well as to protect against amebic liver abscess (ALA). The present work showed the effect of the immunization with PEΔIII-LC3-KDEL3 recombinant protein. In vitro, this candidate vaccine inhibited adherence of E. histolytica trophozoites to HepG2 cell monolayer, avoiding the cytolysis, and in a hamster model, we observed a vaccine-induced protection against the damage to tissue liver and the inhibition of uncontrolled inflammation. PEΔIII-LC3-KDEL3 reduced the expression of TNF-α, IL-1β, and NF-κB in all immunized groups at 4- and 7-day postinfection. The levels of IL-10, FOXP3, and IFN-γ were elevated at 7 days. The immunohistochemistry assay confirmed this result, revealing an elevated quantity of +IFN-γ cells in the liver tissue. ALA formation in hamsters immunized was minimal, and few trophozoites were identified. Hence, immunization with PEΔIII-LC3-KDEL3 herein prevented invasive amebiasis, avoided an acute proinflammatory response, and activated a protective response within a short time. Finally, this recombinant protein induced an increase of serum IgG.
Introducción. Staphylococcus aureus resistente a la meticilina (SARM) es un microorganismo que coloniza las fosas nasales y diferentes partes del cuerpo, lo cual se considera un factor de riesgo para ...adquirir infecciones invasivas, especialmente en pacientes sometidos a cirugía cardiovascular.Objetivo. Determinar la colonización nasal por SARM y establecer las características clínicas en pacientes programados para cirugía cardiovascular.Materiales y métodos. Se hizo un estudio descriptivo entre febrero y diciembre de 2015. Se incluyeron pacientes adultos programados para cirugía cardiovascular en el Hospital Universitario San Ignacio de Bogotá. La colonización se identificó mediante reacción en cadena de la polimerasa (Polymerase Chain Reaction, PCR) en tiempo real en muestras obtenidas mediante hisopados nasales. Los pacientes fueron descolonizados con mupirocina al 2,0 % intranasal dos veces al día y baños con gluconato de clorhexidina al 4 % del cuello hacía abajo durante cinco días, al cabo de lo cual se hizo una PCR de control.Resultados. Se incluyeron 141 pacientes, 52 hospitalizados y 89 ambulatorios. Del total, 19 (13,4 %) tenían colonización nasal por SARM, correspondientes a 9 (17,3 %) de los 52 hospitalizados y 10 (11,2 %) de los 89 ambulatorios. Todos los pacientes sometidos a descolonización tuvieron resultado negativo en la PCR al final del proceso y ninguno presentó infección del sitio operatorio por S. aureus.Conclusiones. Se demostró colonización nasal por SARM tanto en los pacientes hospitalizados como en los ambulatorios. La descolonización con mupirocina fue efectiva para erradicar el estado de portador a corto plazo, lo que podría tener efecto en las tasas de infección del sitio operatorio en las cirugías cardiovasculares.
In this work the results of the isolation of eleven species of chlorophyll microalgae and one cyanobacterium
isolated from industrial residual effluents is presented. It was worked with the species ...Lagerheimia sp. and an
analysis of the harvested algal biomass was performed to identify triacylglycerides by infrared spectroscopy and
gas chromatography. It was determined that this organism shows a great capacity for diminishing the COD
(oxygen chemical demand) until 43.17 % in wastewater samples with an initial 1 199 mg/L COD values. 20.4%
of palmitic acid, 16.07% of elaidic acid, 18.8% of linoleic acid and 20.04% of gamma-linoleic acid are reported.
Microalgae cultures isolated from Altamira industrial zone represent an alternative treatment for wastewaters as
well as a potentially biodiesel source.
En este trabajo se presentan los resultados del aislamiento de once especies de microalgas clorofitas y una
cianobacteria aisladas de efluentes residuales industriales. Se trabajó con la especie Lagerheimia sp. y se realizó
un análisis de la biomasa algal cosechada, para identificar triacilglicéridos por espectroscopía infrarroja y por
cromatografía de gases. Se determinó que la especie tiene una capacidad de remoción de la DQO de hasta
43.17 % con valores iniciales de DQO en el orden de 1 199 mg/L y se reporta 26.4% de ácido palmítico,
16.07% de ácido elaídico, 18.80% de ácido linoleico y 20.4% de ácido gama linoleico. Los cultivos de microalgas
obtenidos de la zona industrial de Altamira representan una alternativa para la biorremediación de estas aguas
residuales industriales a la par de la obtención de metabolitos de interés como los lípidos para la síntesis de
biodiesel.
Painful ulcerated plaque on the back Zolano-Orozco, R. Miroslava; Mendez-Flores, Raúl G.; Contreras-González, Diana M. ...
JAAD Case Reports,
06/2021, Volume:
12
Journal Article
Display omitted
► Synthesis of a novel polydiacetylene containing tolan units as NLO-chromophores. ► Quadratic and cubic NLO-characterizations were performed in prepared film samples. ► Outstanding ...NLO-coefficients were evaluated via the Z-Scan and SHG-techniques. ► High Tg-values of this polymer indicates suitable properties for material processing.
A new polymer, poly(p-propargyloxybenzoate) containing a polar tolan as nonlinear optically active chromophore was synthesized and characterized. This polymer shows a Tg-value of ∼98°C and is soluble in typical organic solvents. These properties allowed us to fabricate mechanically stable and high quality semi-transparent thin films by spin coating of N-methylpirrolidone based solutions; obtained samples were adequate for linear and nonlinear photophysical characterizations. In order to explore the quadratic nonlinear optical (NLO) properties, molecular chromophore ordering was electrically induced within the polymeric films via the corona poling method. According to the Maker fringes technique, oriented films with an order parameter of ∼0.25 were able to display large and stable off-resonant second-order NLO-effects such as second harmonic generation (SHG, @ 532nm); the estimated χzzz(2) and χzxx(2) macroscopic NLO-coefficients were in the order of 186.8 and 71.2pmV−1, respectively. On the other hand, the cubic NLO-properties of these films were also studied via the Z-Scan technique, where the deposited films displayed an outstanding NLO refractive (n2) and absorptive (β) performance. Indeed, due to the high conjugation degree and intra-molecular charge transfer (ICT) properties, poled and unpoled polymer films displayed huge n2-values in the order of 10−8m2W−1 with negative sign and NLO saturable absorption β-coefficients in the order of 10−1mW−1. The large quadratic and cubic NLO-coefficients obtained for this novel polymer evidence its potentiality in the development of organic-based opto-electronic and photonic prototypes.
B lymphocytes are a leukocyte subset capable of developing several functions apart from differentiating into antibody‐secreting cells. These processes are triggered by external activation signals ...that induce changes in the plasma membrane properties, regulated by the formation of different lipid‐bilayer subdomains that are associated with the underlying cytoskeleton through different linker molecules, thus allowing the functional specialization of regions within the membrane. Among these, there are tetraspanin‐enriched domains. Tetraspanins constitute a superfamily of transmembrane proteins that establish lateral associations with other molecules, determining its activity and localization. In this study, we identified TSPAN33 as an active player during B‐lymphocyte cytoskeleton and plasma membrane‐related phenomena, including protrusion formation, adhesion, phagocytosis, and cell motility. By using an overexpression model of TSPAN33 in human Raji cells, we detected a specific distribution of this protein that includes membrane microvilli, the Golgi apparatus, and extracellular vesicles. Additionally, we identified diminished phagocytic ability and altered cell adhesion properties due to the aberrant expression of integrins. Accordingly, these cells presented an enhanced migratory phenotype, as shown by its augmented chemotaxis and invasion rates. When we evaluated the mechanic response of cells during fibronectin‐induced spreading, we found that TSPAN33 expression inhibited changes in roughness and membrane tension. Contrariwise, TSPAN33 knockdown cells displayed opposite phenotypes to those observed in the overexpression model. Altogether, our data indicate that TSPAN33 represents a regulatory element of the adhesion and migration of B lymphocytes, suggesting a novel implication of this tetraspanin in the control of the mechanical properties of their plasma membrane.
Tetraspanins constitute a superfamily of transmembrane proteins that establish lateral associations with other molecules, determining its activity and localization. In this study, we identified the last described member of this family, tetraspanin 33 (TSPAN33), as an active player during B‐lymphocyte cytoskeleton and plasma membrane‐related phenomena, including protrusion formation, adhesion, phagocytosis, and cell motility.
Abstract Cancer represents the second leading cause of death worldwide, therefore, the search for chemoprotective agents is on the rise. The muscle of white shrimp (Litopenaeus vannamei) is one of ...the species analyzed as it has been reported as a source of compounds with antiproliferative activity. The aim of this study was to evaluate the effect of shrimp muscle-isolated compounds on cell proliferation and morphology of human cancer cell lines. The muscle underwent a process of extraction and fractionation of compounds; their effect on cell viability assays (MTT) on lung adenocarcinoma (A549), prostate carcinoma (22 Rv-1), invasive breast adenocarcinoma (MDA MB 231), colon carcinoma (HTC 116), cervix adenocarcinoma (HeLa) and non-cancerous retinal cells (ARPE-19) was measured. Morphological changes were observed using fluorescence microscopy and chemical structure data was obtained using nuclear magnetic resonance. Fraction named C5 showed the highest antiproliferative activity on HCT-116 and MDA-MB-231, without significantly affecting the control cells. Subfractions C5-3 and C5-4 presented significant antiproliferative potential in MDA-MB-231; this cell line showed morphological changes that could be related to apoptosis, and spectroscopic analysis revealed the presence of b-carotene and eicosapentaenoic acid in C5, nevertheless, further studies are needed to determine the effect of each compound.
Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) is a microorganism that colonizes nostrils and different parts of the body, which is considered a risk factor to acquire invasive ...infections, especially in cardiovascular surgery patients.
Objective: To determine the frequency of nasal colonization by MRSA and to establish the clinical characteristics in patients scheduled for cardiovascular surgery.
Materials and methods: This was a descriptive study conducted between February and December, 2015. We included adult patients scheduled for cardiovascular surgery at the Hospital Universitario San Ignacio in Bogotá, Colombia. Colonization was identified by real-time PCR from nasal swabs. Colonized patients were treated with mupirocin 2.0% intranasally twice a day and bathed with chlorhexidine 4% from the neck downwards for five days. At the end of this treatment, PCR control was carried out.
Results: We included 141 patients with a percentage of nasal colonization of 13.4% (19/141). There were 52 hospitalized patients and 89 outpatients with a percentage of nasal colonization of 17.3% (9/52), and 11.2% (10/89), respectively. All colonized patients who received treatment had a negative PCR at the end of the regime and none of the participating patients had a surgical site infection by S. aureus at the end of the study.
Conclusions: Nasal colonization was observed both in hospitalized patients and outpatients. Decolonization treatment with mupirocin was effective to eradicate the carrier state in the short term, which could impact the rates of surgical wound infection associated with cardiovascular surgery.
La Gestión del Conocimiento en la Nube (GCN) es un constructo teórico que conjuga la dimensión organizacional (Gestión del Conocimiento, GC), la dimensión tecnológica (Computación en la Nube, CN) y ...la dimensión académica como contexto de aplicación. Para llegar a una definición conceptual válida del constructo propuesto, resulta pertinente primero abordar las concepciones teóricas fundamentales de los dominios involucrados. La GC es la disciplina que se encarga del estudio de las herramientas tecnológicas, las técnicas y procedimientos organizativos y los cambios en la gestión de las personas que permiten desarrollar y extraer el máximo nivel de aprovechamiento del conocimiento de una organización. A su vez, la CN toma la tecnología, los servicios y las aplicaciones que son similares a los de Internet y los convierte en una utilidad de autoservicio. En la convergencia entre los dominios organizacional y de sistemas de información (SI), la intención de esta propuesta es proclamar a la GCN como una nueva categoría estratégica en el contexto de las organizaciones académicas que tienen acceso a los beneficios del desarrollo evolutivo de los SI. Así, en esta convergencia entre los tres campos de investigación definidos, la GC, la CN y la academia como contexto de aplicación, se ha identificado una frontera de conocimiento factible, así como la oportunidad de abonar una aportación teórica creativa. Se propone entonces el concepto de la GCN conjugando los modelos de referencia de la GC y la CN y se discute su importancia y sus implicaciones estratégicas en las organizaciones académicas. Abstract Knowledge Management in the Cloud (KMC) is a theoretical construct that combines the organizational dimension (Knowledge Management, KM), the technological dimension (Cloud Computing, CC) and the academic dimension as the context application. For a valid definition of the proposed concept, it was pertinent to address the fundamental theoretical conceptions of the involved domains. KM is the discipline that is responsible for the study of technological tools, techniques, organizational procedures and changes in the management of people that allow the development and extraction of the maximum level of knowledge use in an organization. On the other hand, the CC takes Internet-like technology services and applications and turns them into a self-service utility. At the convergence between organizational and information systems approaches, the purpose of this proposal is to establish the KMC as a new strategic category in the context of academic organizations that have access to the benefits of the information technologies evolution and development. At the established convergence between the three mentioned fields of research, a feasible knowledge frontier was identified with the opportunity to suggest a creative theoretical contribution. The concept of KMC is then proposed, combining the KM reference model that defines it as a set of strategic processes with the standardized CC services model. Finally, its importance and strategic implications in academic organizations are discussed.
PDF
