We present the PMAS/PPak Integral-field Supernova hosts COmpilation (PISCO), which comprises integral field spectroscopy (IFS) of 232 supernova (SN) host galaxies that hosted 272 SNe, observed over ...several semesters with the 3.5 m telescope at the Calar Alto Observatory (CAHA). PISCO is the largest collection of SN host galaxies observed with wide-field IFS, totaling 466,347 individual spectra covering a typical spatial resolution of ∼380 pc. Focused studies regarding specific SN Ia-related topics will be published elsewhere; this paper aims to present the properties of the SN environments, using stellar population (SP) synthesis, and the gas-phase interstellar medium, providing additional results separating stripped-envelope SNe into their subtypes. With 11,270 H ii regions detected in all galaxies, we present for the first time a statistical analysis of H ii regions, which puts H ii regions that have hosted SNe in context with all other star-forming clumps within their galaxies. SNe Ic are associated with environments that are more metal-rich and have higher EW(H ) and higher star formation rate within their host galaxies than the mean of all H ii regions detected within each host. This in contrast to SNe IIb, which occur in environments that are very different compared to other core-collapse SNe types. We find two clear components of young and old SPs at SNe IIn locations. We find that SNe II fast decliners tend to explode at locations where the SFR is more intense. Finally, we outline how a future dedicated IFS survey of galaxies in parallel to an untargeted SN search would overcome the biases in current environmental studies.
We present a fully consistent catalog of local and global properties of host galaxies of 882 Type Ia supernovæ (SNIa) that were selected based on their light-curve properties, spanning the redshift ...range 0.01 < z < 1. This catalog corresponds to a preliminary version of the compilation sample and includes Supernova Legacy Survey (SNLS) 5-year data, Sloan Digital Sky Survey (SDSS), and low-redshift surveys. We measured low- and moderate-redshift host galaxy photometry in SDSS stacked and single-epoch images and used spectral energy distribution fitting techniques to derive host properties such as stellar mass and U − V rest-frame colors; the latter are an indicator of the luminosity-weighted age of the stellar population in a galaxy. We combined these results with high-redshift host photometry from the SNLS survey and thus obtained a consistent catalog of host stellar masses and colors across a wide redshift range. We also estimated the local observed fluxes at the supernova location within a proper distance radius of 3 kpc, corresponding to the SNLS imaging resolution, and transposed them into local U − V rest-frame colors. This is the first time that local environments surrounding SNIa have been measured at redshifts spanning the entire Hubble diagram. Selecting SNIa based on host photometry quality, we then performed cosmological fits using local color as a third standardization variable, for which we split the sample at the median value. We find a local color step significance of − 0.091 ± 0.013 mag (7σ), which effect is as significant as the maximum mass step effect. This indicates that the remaining luminosity variations in SNIa samples can be reduced with a third standardization variable that takes the environment into account. Correcting for the maximum mass step correction of − 0.094 ± 0.013 mag, we find a local color effect of − 0.057 ± 0.012 mag (5σ), which shows that additional information is provided by the close environment of SNIa. Departures from the initial choices were investigated and showed that the local color effect is still present, although less pronounced. We discuss the possible implications for cosmology and find that using the local color in place of the stellar mass results in a change in the measured value of the dark energy equation-of-state parameter of 0.6%. Standardization using local U − V color in addition to stretch and color reduces the total dispersion in the Hubble diagram from 0.15 to 0.14 mag. This will be of tremendous importance for the forthcoming SNIa surveys, and in particular for the Large Synoptic Survey Telescope (LSST), for which uncertainties on the dark energy equation of state will be comparable to the effects reported here.
Some feedstuffs containing significant levels of fiber may be a good source of bioactive compounds that may contribute to improving broiler meat quality. However, high fiber level can have a negative ...impact on broiler performance. A study was undertaken to investigate the impact of incorporating citrus pulp (5 or 10%) or dehydrated pasture (5 or 10%) on the performance, carcass yield, and characteristics of broiler chickens. A diet containing neither citrus pulp nor dehydrated pasture was used as control. The results on growth performances showed that daily weight gain was reduced by 26% in birds of the 10% citrus pulp treatment (P < 0.05). Compared with the control treatment, increases in feed intake occurred in birds consuming diets with 5 or 10% citrus pulp, which resulted in significantly higher feed conversion rates with the 10% level. Under the same incorporation rate, dehydrated pasture had effects less evident on the performances of broiler chicken. In addition, diets containing citrus pulp, displaying higher percentages of soluble nonstarch polysaccharides, increased small intestine relative length, and reduced carcass yield. Inclusion of 10% dehydrated pasture in diets resulted in improved breast skin yellowness (P < 0.05). Finally, the results revealed that incorporation of the nonstarch polysaccharide-rich feedstuffs had a major impact on the fatty acid profile (affected 16 of 21 fatty acids) of broiler meat. Polyunsaturated fatty acids content in meat was higher in birds consuming the highest levels of both citrus pulp and dehydrated pasture, leading to increased ratios of polyunsaturated to saturated fatty acids. Together, the results suggest that incorporation of moderate levels of dehydrated pastures in poultry diets has a minor impact on broiler performance and can contribute significantly to improve breast skin yellowness and fatty acid composition of meat.
We describe the synthesis of water-soluble molecularly imprinted polymer nanoparticles (MIP-NPs) as a new artificial host receptor for the recognition of adenosine monophosphate (AMP), used herein, ...as a model nucleotide. MIP-NPs were prepared by solid-phase synthesis on glass beads (GB) using, for the first time, immobilized Fe(III)-chelate as an affinity ligand to orientate the AMP via its phosphate group. A polymerizable thymine monomer which can induce complementary base-pairing with the adenine moiety of the nucleotide was synthesized and incorporated in the polymerization mixture to constrain the AMP in a dual-orientated configuration. The MIP-NPs were remarkably selective toward AMP as they did not bind other nucleotides, GMP, UMP, and CMP. This strategy of using the phosphate group of AMP as a hinge enables unhindered pairing of the nucleobase with its corresponding complementary base monomer and can be extended to the preparation of specific MIP receptors for other key nucleotides in aqueous conditions.
The objective was to evaluate the structural and functional quality of bull sperm after sexing by flow cytometry. Frozen non-sexed (NS), sexed for X (SX) and sexed for Y (SY) sperm from four bulls ...was used. Frozen-thawed sperm was analyzed for motility, sperm head agglutination, morphology, capacitation, and integrity of the plasma membrane, acrosome, and chromatin. After Percoll centrifugation (45:60% gradients), the pellet was used for sperm analysis or IVF. Data were analyzed using generalized linear models (P < 0.05) and were reported as least squares means ± standard error (SEM). Based on sperm evaluations, NS sperm had better (P < 0.05) quality than sexed sperm, including higher motility and greater percentages of cells with an intact membrane and acrosome (58.0 ± 3.0, 58.2 ± 3.0, and 60.9 ± 3.3) than SX (29.6 ± 1.3, 36.0 ± 2.9, and 37.1 ± 3.3), and SY (26.2 ± 2.1, 36.4 ± 2.9, and 37.5 ± 3.3). There were no differences (P > 0.05) among groups for fertilization and cleavage rates. Similarly, blastocyst rate on Day 8 (Day 0 = day of insemination) did not differ among groups (22.2 ± 3.2, 18.1 ± 3.3, and 14.8 ± 2.9 for NS, SX, and SY, respectively). Regarding embryo development kinetics, all groups had similar developmental stages from Days 6 to 9. Although the sex-sorting procedure affected sperm characteristics, it did not significantly affect fertilization or embryo development.
Abstract
In recent years, automatic classifiers of image cutouts (also called “stamps”) have been shown to be key for fast supernova discovery. The Vera C. Rubin Observatory will distribute about ten ...million alerts with their respective stamps each night, enabling the discovery of approximately one million supernovae each year. A growing source of confusion for these classifiers is the presence of satellite glints, sequences of point-like sources produced by rotating satellites or debris. The currently planned Rubin stamps will have a size smaller than the typical separation between these point sources. Thus, a larger field-of-view stamp could enable the automatic identification of these sources. However, the distribution of larger stamps would be limited by network bandwidth restrictions. We evaluate the impact of using image stamps of different angular sizes and resolutions for the fast classification of events (active galactic nuclei, asteroids, bogus, satellites, supernovae, and variable stars), using data from the Zwicky Transient Facility. We compare four scenarios: three with the same number of pixels (small field of view with high resolution, large field of view with low resolution, and a multiscale proposal) and a scenario with the full stamp that has a larger field of view and higher resolution. Compared to small field-of-view stamps, our multiscale strategy reduces misclassifications of satellites as asteroids or supernovae, performing on par with high-resolution stamps that are 15 times heavier. We encourage Rubin and its Science Collaborations to consider the benefits of implementing multiscale stamps as a possible update to the alert specification.
Comparisons between venoms from snakes kept under captivity or collected at the natural environment are of fundamental importance in order to obtain effective antivenoms to treat human victims of ...snakebites. In this study, we compared composition and biological activities of Bothrops atrox venom from snakes collected at Tapajós National Forest (Pará State, Brazil) or maintained for more than 10 years under captivity at Instituto Butantan herpetarium after have been collected mostly at Maranhão State, Brazil. Venoms from captive or wild snakes were similar except for small quantitative differences detected in peaks correspondent to phospholipases A2 (PLA2), snake venom metalloproteinases (SVMP) class PI and serine proteinases (SVSP), which did not correlate with fibrinolytic and coagulant activities (induced by PI-SVMPs and SVSPs). In both pools, the major toxic component corresponded to PIII-SVMPs, which were isolated and characterized. The characterization by mass spectrometry of both samples identified peptides that matched with a single PIII-SVMP cDNA characterized by transcriptomics, named Batroxrhagin. Sequence alignments show a strong similarity between Batroxrhagin and Jararhagin (96%). Batroxrhagin samples isolated from venoms of wild or captive snakes were not pro-coagulant, but inhibited collagen-induced platelet-aggregation, and induced hemorrhage and fibrin lysis with similar doses. Results suggest that in spite of environmental differences, venom variability was detected only among the less abundant components. In opposition, the most abundant toxin, which is a PIII-SVMP related to the key effects of the venom, is structurally conserved in the venoms. This observation is relevant for explaining the efficacy of antivenoms produced with venoms from captive snakes in human accidents inflicted at distinct natural environments.
•Bothrops atrox venom from Brazilian wild snakes or kept under captivity were compared.•Variability in protein composition was observed only in the less abundant components.•Differences in venom composition did not correlate to major venom functions.•Venoms shared the same major toxin: Bathoxrhagin, a hemorrhagic metalloproteinase.
Free-range chickens are assumed to consume low to moderate levels of pasture, although the effects of forage intake in broiler performance and poultry meat quality remain to be established. In ...addition, despite cellulases and hemicellulases being widely used as feed supplements to improve the nutritive value of cereal-based diets for fast-growing broilers, the potential interest of these biocatalysts in the production of free-range chicken is yet to be established. In this study, broilers of the RedBro Cou Nu × RedBro M genotype were fed a cereal-based diet in portable floorless pens located either on a rainfed subterranean clover (Trifolium subterraneum) pasture or on an irrigated white clover (Trifolium repens) pasture. Control birds were maintained at the same site in identical pens but with no access to pastures. The importance of pasture intake and enzyme supplementation in the performance and meat sensory properties of the free-range chicken from d 28 to 56 was investigated. The results revealed that although cellulase and hemicellulase supplementation had no impact on broiler performance (P > 0.05), birds foraging on legume-based pastures reached significantly greater final BW. The data suggest that the improvement in broiler performance results from increased intake of the cereal-based feed rather than from an improvement in the efficiency of nutrient utilization per se. Interestingly, although the intake of the subterranean clover pasture had no impact on the tenderness, juiciness, and flavor of broiler meat, members of a 30-person consumer panel classified the meat from grazing broilers with greater scores for overall appreciation. Together, the results suggest that pasture intake promotes bird performance while contributing to the production of broiler meat with preferred sensory attributes.
Essentials
Bovine (HBI) and porcine (HPI) heparins differ in structure and anticoagulant activity.
Protamine‐neutralization was evaluated on a variety of physical‐chemical methods.
HBI requires more ...protamine than HPI to fully neutralize its anticoagulant activity.
Protamine preferentially removes higher‐sulfated chains of HBI while HPI is evenly precipitated.
Summary
Background
Protamine neutralization is an essential step for the safe use and inactivation of the unfractionated heparin (UFH) that is widely employed in surgical and non‐surgical procedures involving extracorporeal circulation.
Objective
To compare protamine neutralization of different pharmaceutical‐grade UFHs prepared from porcine or bovine intestine (HPI and HBI, respectively). HBI has approximately half the anticoagulant potency of HPI, mostly as consequence of its fraction enriched with N‐sulfated α‐glucosamine disaccharides.
Methods
Protamine neutralization of HPI and HBI was evaluated with in vitro, ex vivo and in vivo assays. We also performed in‐depth assessments of the complexation of protamine with these distinct UFHs by using nuclear magnetic resonance and mass spectroscopy.
Results
HPI and HBI interact similarly with protamine on a mass/mass basis; however, HBI requires more protamine than HPI to have its anticoagulant activity fully neutralized, because of its lower potency, which entails the use of higher doses. Nuclear magnetic resonance spectra revealed that HPI precipitates homogeneously with protamine. On the other hand, the low‐sulfated fraction of HBI, enriched with N‐sulfated α‐glucosamine, precipitates at higher concentrations of protamine than the fraction more like HPI, with a preponderance of N,6‐disulfated α‐glucosamine disaccharides. Finally, mass spectroscopy spectra showed that some of the different peptide components of protamine interact preferentially with the heparins, irrespective of their animal origin.
Conclusion
Our results have important medical implications, indicating that protamine neutralization of HBI, determined exclusively by point‐of‐care coagulation assessments, must fail because of its lower‐sulfated fraction with reduced anticoagulant activity that could remain in the circulation after the neutralization procedure.
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