Razmnoževanje s potaknjenci je najpomembnejša in najpogosteje uporabljena metoda vegetativnega razmnoževanja rastlin v okrasnem vrtnarstvu. V verigi proizvodnje sadik se pridelovalci poslužujejo ...različnih tehnik in metod, da bi zagotovili oziroma ohranili karseda kakovosten rastlinski material. Zaradi selitve večjih svetovnih pridelovalcev zelnatih okrasnih rastlin v tropska in subtropska območja, kjer je pridelava enostavnejša, cenejša in lažja, je ohranjanje kakovosti materiala v času transporta postalo ključnega pomena za nadaljnjo proizvodnjo rastlin. Metoda hladnega skladiščenja se uporablja pri vegetativnem razmnoževanju in pri transportu potaknjencev zelnatih in lesnatih okrasnih rastlin iz ekvatorialnih delov do območij, kjer jih nato koreninijo. Hladno skladiščenje zniža temperaturo rastlinskega materiala, posledično se upočasni metabolizem rastlin med skladiščenjem, ohrani se rastni potencial in kakovost potaknjencev ter podaljša se njihovo obstojnost. V prispevku je predstavljen pregled na področju hladnega skladiščenja potaknjencev različnih vrst okrasnih rastlin, s poudarkom na potaknjencih zelnatih, lesnatih rastlin in rastlin iz in vitro proizvodnje.
The aim of this study was to investigate the differences between Castanea sativa Mill. and Castanea crenata Siebold & Zucc. × Castanea sativa Mill. in rooting ability in relation to endogenous levels ...of auxin, auxin cofactors and inhibitors that influence rooting success. Leafy cuttings of the two commercial cultivars ‘Marsol’ and ‘Maraval’ (Castanea crenata × Castanea sativa) and the native accession ‘Kozjak’ (Castanea sativa) were analyzed. Endogenous indole-3-acetic acid (IAA) concentration was assessed at the beginning of propagation (day 0); in addition, strigolactones, flavonoids, rooting ability and quality were assessed 120 days after. The concentration of endogenous IAA in ‘Maraval’ (324.34 ± 28.66 ng g−1) and ‘Marsol’ (251.60 ± 35.44 ng g−1) was significantly higher than in ‘Kozjak’ (112.87 ± 35.44 ng g−1). The best rooting result was observed with the genotypes ‘Maraval’ (100.00 ± 0.00%) and ‘Marsol’ (90.48 ± 6.15%). A significantly lower strigol concentration was observed in the roots of ‘Maraval’ (75.54 ± 17.93 ng g−1) compared with other genotypes. The total flavonoid concentration in ‘Maraval’ was significantly higher (2794.99 ± 187.13 μg g−1) than in ‘Kozjak’ (1057.38 ± 61.05 μg g−1). Our results indicate that the concentration of endogenous IAA has a significant influence on rooting success. The results further indicate that in the case of flavonoids and strigolactones, not only the individual compounds but also their ratio is important for rooting success. Correlation coefficients calculated between analyzed compounds and rooting success point toward specific functions of flavonoids and strigolactones in the rooting of Castanea that need to be functionally analyzed.
The aim of this study was to investigate the uptake of exogenously added indole-3-butyric acid (IBA) as an adventitious root (AR) inducer. The concentration of indole-3-butyric acid (IBA) in leafy ...cuttings of Prunus subhirtella ‘Autumnalis’ from stock material of different physiological ages was analyzed at three evaluation time points (1, 4, 24 h). We examined three stock plants: a physiologically mature stock plant (approximately 60 years old); physiologically rejuvenated plants (16 years old) that were previously propagated by cuttings from semi-mature stock material; and in vitro juvenile stock material. For IBA to effectively induce AR formation, it must first be converted into the active hormone indole-3-acetic acid (IAA). The total concentration of IAA in the first 24 h after cutting was evaluated according to the physiological age of the stock material and the IAA concentration in the cuttings according to the evaluation time points was assessed. We also examined rooting success and the quality of the adventitious root system depending on the physiological age of the stock material. High performance liquid chromatography coupled with mass spectrometry (HPLC-MS/MS) was used to identify and quantify the phytohormones. The difference in rooting and quality of the developed AR was observed between semi-mature stock and mature stock material. Cuttings from semi-mature plants rooted in 95.00 ± 5.00% of cases, while cuttings from mature stock plants only rooted in 68.33 ± 4.09%. Successfully rooted cuttings from mature stock material were accompanied by callus formation, which was significantly lower in cuttings from semi-mature stock material. Our results show that the interaction between physiological age and time after severance had no effect on IBA concentration in the cuttings (p = 0.907). Duration of time elapsed since severing (evaluation time points) had a significant effect on the uptake of IBA (p = 0.002 **) and IAA (p = 0.0009 ***) in the cuttings. Physiological age had a significant effect on IAA concentration in the early stages of AR formation in the cuttings (p = 0.038 *). Our results suggest that the dynamics of other endogenous phytohormones and the physiological state of the stock plant are also important for AR formation, as the proportion of successfully rooted cuttings from semi-mature stock material was significantly higher than the proportion of successfully rooted cuttings from mature stock material, irrespective of IBA uptake in stock material of different ages.
The influence of mycorrhizal inoculum in combination with different phosphorus treatments on growth and flowering parameters of Ajania (Ajania pacifica (Nakai) Bremer et Humphries) plants was ...investigated in two growing seasons (2015 and 2016). Plants of the cultivar ‘Silver and Gold’ were transplanted into pots either with added mycorrhizal inoculum or without inoculum and assigned to four phosphorus treatments. Mycorrhizal colonization was assessed by evaluating the frequency of colonization, intensity of colonization and density of fungal structures (arbuscules, vesicles, coils and microsclerotia) in the roots. During the growing season, the content of plant available phosphorus in the soil was analyzed, and shoot length, number of shoots, number of inflorescences, number of flowers and flowering time were evaluated. Inoculated Ajania plants were successfully colonized with arbuscular mycorrhizal fungi and dark septate endophytic fungi. In the root segments, hyphae were mainly observed, as well as vesicles, coils, arbuscules and microsclerotia, but in lower density. The density of fungal structures did not differ among phosphorus treatments, but did differ between years, with a higher density of fungal structures in 2016. Mycorrhizal plants developed higher number of shoots in 2016, higher number of inflorescences, higher number of flowers, and they flowered longer compared to uninoculated plants.
The aim of the study was to determine the content and composition of phenolic compounds in autochthonous rose hips (
,
and
) and to compare them with the content of phenolic compounds in their ...cultivars (‘Harstad’, ‘Bourgogne’, ‘Mount Everest’, ‘Poppius’, ‘Frühlingsduft’, ‘Single Cherry’, ‘Frühlingsmorgen’, ‘ Violacea’ and ‘Splendens’) collected in Arboretum Volčji Potok in Slovenia. The total content of phenolic compounds in the flesh with skin was lowest in ‘Mount Everest’ (3603.57 mg · kg
f.w.), which is derived from
, and highest in ‘Splendens’ (68789.39 mg · g
f.w.), which is derived from
. In seeds, the phenolic compound content was lower, as expected, ranging from 757.02 mg · kg
f.w. (‘Mount Everest’) to 6823.21 mg · kg
f.w. (‘Single Cherry’). It can be concluded that the cultivars differ significantly from each other due to the content of different bioactive compounds. Based on the obtained results, we cannot determine to which basic rose plant the cultivar belongs based only on the content of one analysed compound. The contents were very different because only one parent plant is known for a given cultivar and breeding programmes in roses were very narrowly focussed on specific targets.
The aim of our study was to determine how the content and composition of volatile organic compounds (VOCs) change with crossbreeding. We included three autochthonous Slovenian species of roses (R. ...pendulina, R. spinosissima, and R. gallica) and several cultivars derived from them in the research. The results were compared with the content of the reference plant R. damascena, which is known worldwide for its rich VOC profile and is used in the perfume industry. We found that the total VOC content ranged from 2.579 µg/g FW (‘Fruhlingsmorgen’) to 168.24 µg/g FW (‘Violacea’). According to the content of total VOCs, the variety ‘Charles de Mills’ (43.12 µg/g DM) was the most similar to the variety R. damascena (44.55 µg/g DM). We can conclude that the flowers of the roses we included in the study are a rich source of VOCs. VOC content in hybrids varies depending on the purpose and goals of breeders. We also found that flowering time had no effect on VOC content and composition. With this study, we not only carefully examined the VOC profile of roses but also clearly showed which compounds were present in individual taxa. In this way, it is possible to select a suitable variety or cultivar depending on the desired use, for example, for flavoring food or for a floral fragrance in the perfume industry.
This study aimed to determine the content and composition of bioactive compounds in autochthonous rose hips (R. pendulina, R. spinosissima, and R. gallica) and to compare them with the content of ...bioactive compounds in some cultivars (‘Harstad’, ‘Bourgogne’, ‘Mount Everest’, ‘Poppius’, ‘Fruhlingsduft’, ‘Single Cherry’, ‘Fruhlingsmorgen’, ‘Violacea’, and ‘Splendens’) derived from these main species. Due to insufficient information on how bioactive compound content changes when crossing roses, this study also sought to ascertain whether modern rose hip cultivars are still a sufficiently rich source of bioactive compounds and could, therefore, be potentially used as a functional food. All material was collected in the Arboretum Volčji Potok (Slovenia). The ascorbic acid content was highest in the ‘Harstad’ cultivar (12.79 g/kg FW), and the total organic acid content varied from 1.57 g/kg FW (R. spinosissima) to 34.39 g/kg FW (‘Harstad’). Of all the carotenoids analyzed, only lycopene and β-carotene were present in all the samples. The total carotenoid content was highest in the ‘Fruhlingsmorgen’ cultivar (100.84 mg/kg FW), derived from R. spinosissima, and lowest in the main species, R. spinosissima (9.26 mg/kg FW). It can be concluded, therefore, that the content of bioactive compounds in rose hips of modern cultivars is generally lower than in rose hips of old cultivars and original species included in this study. The research results confirm that modern breeding strategies are mainly focused on goals such as abundant flowering and resistance to diseases and pests and not so much on the content of bioactive compounds.
Zusammenfassung
In den Jahren 2004 bis 2006 wurden jeweils im Sommer an der Universität Ljubljana Versuche zur Bewurzelung von Grünstecklingen der Birnensorte ‘Uta’ durchgeführt. Dabei bewurzelten ...zwischen 6,1 und 23,8 % der Langstecklinge. Im Herbst 2007 wurden die so erhaltenen wurzelechten Bäume im Versuchszentrum der Universität für Bodenkultur in Wien gepflanzt und mit wurzelechten Bäumen der Sorte ‘Uta’ aus in vitro-Vermehrung und mit auf Kirchensaller Mostbirne (Sämling) und Fox 11 veredelten verglichen. Alle Bäume wurden als Spindel erzogen und nach Bio-Anbau-Richtlinien gepflegt. Die wurzelechten Bäume kamen später in den Ertrag als die veredelten. Nach elf Jahren wiesen die Bäume auf Sämling den stärksten Wuchs und Ertrag auf, gefolgt von den Veredelungen auf Fox 11 und den wurzelechten Bäumen aus in vitro Vermehrung. Deutlich schwächer wuchsen die wurzelecht vermehrten Grünstecklinge. Keine Unterschiede gab es beim spezifischen Ertrag, der bei allen Varianten im Bereich zwischen 1,1 und 1,2 kg/cm
2
lag. Die nicht veredelten Bäume wiesen tendenziell größere Früchte als die beiden veredelten Varianten auf; speziell bei Fox 11 waren die Früchte kleiner. Wichtig bei den wurzelechten Bäumen ist die Verwendung kräftigerer Bäume bei der Pflanzung und die Durchführung einer Herbstpflanzung, um Baumausfälle in den ersten Jahren zu vermeiden.
The genus
is very extensive and variable, so it remains very unpredictable and uninvestigated. This also holds true for values of secondary metabolites in rose hips, which are important for several ...purposes (human diet, protection of plants against pests, etc.). The aim of our study was to determine the content of phenolic compounds in the hips of
×
,
,
and
, which grow wild in nature in southwestern Slovenia. We examined the content of phenolic compounds in different parts of rose hips, in the flesh with skin and in the seeds, depending on the individual species, over a period of two years, 2020 and 2021. We also considered the influence of environmental conditions on the content of the mentioned compounds. In both years, the content of phenolic compounds was higher in the flesh with skin than in the seeds. Considering the total content of phenolic compounds in the flesh with skin,
stands out (15,767.21 mg/kg FW), but the hips of this species accumulated the lowest number of different phenolic compounds. The lowest content of total phenolic compounds (TPC) was found in
in the year 2021 (3501.38 mg/kg FW). The content of TPC (in both observed years) in the seeds varied between 1263.08 mg/kg FW (
) and 3247.89 mg/kg FW (
×
). Among the anthocyanins, cyanidin-3-glucoside was determined, which was predominant in
(28.78 mg/kg FW), and at least was determined in
(1.13 mg/kg FW). When comparing the two years of the period (2020-2021), we found that 2021 was more favorable for the formation of phenolic compounds in the seeds, but 2020 in the flesh with skin.
Our research aimed to investigate the primary and secondary metabolites of rosehips and petals of R. gallica in comparison with R. subcanina. R. gallica was chosen because it is still unexplored in ...terms of various bioactive substances and is strongly present in Slovenia. Given that roses are generally very variable and unstudied, our research will contribute to greater transparency and knowledge of the bioactive composition of rosehips and petals. We found a strong positive correlation between the total content of phenolics and ascorbic acid, between the total content of organic acids and the total content of carotenoids, and between the total content of sugars and the total content of organic acids. Hips of R. gallica contained higher amounts of sugars, ascorbic acid, and carotenoids than R. subcanina. Based on the composition of phenolic compounds in the petals, it is possible to distinguish between the two species. Among all the phenolic compounds in the petals, both genotypes are richest in gallotannins, followed by flavonols. Among anthocyanins, cyanidin-3-glucoside was determined, the content of which was also higher in R. gallica. It can be concluded that the studied hips had an extremely low sugar content and, consequently, an extremely high organic acid content. The content of carotenoids in hips was in the lower range of the average content compared to data from the literature. By optimizing the harvesting time, we could obtain a higher content of carotenoids, which could potentially be used for industrial purposes. However, we found that the analyzed petals were a rich source of phenolic compounds, which benefit the human body and could be potentially used in the food and cosmetic industries.
PDF
