Lapa do Santo rockshelter, a Paleoindian site located in Eastern-Central Brazil, presented two main occupations; one during Early Holocene (12,460 to 8700 cal BP), and a latter in Middle Holocene ...(5100 to 4200 cal BP). In spite of this 3600 year gap, the stratigraphy and general characteristics of the material culture did not indicate any visible discontinuity. This led us to hypothesise a reoccupation of the rockshelter by the same cultural group, tested by means of statistical analyses comparing lithics, bone artifacts, and faunal remains from early and middle Holocene layers. No significant differences were found, and our results indicate the presence of a cultural tradition that persisted for 8240 years, or roughly 412 generations.
General aspects of muscle glucose uptake Alvim, Rafael O; Cheuhen, Marcel R; Machado, Silmara R ...
Anais da Academia Brasileira de Ciências,
03/2015, Volume:
87, Issue:
1
Journal Article
Peer reviewed
Open access
Glucose uptake in peripheral tissues is dependent on the translocation of GLUT4 glucose transporters to the plasma membrane. Studies have shown the existence of two major signaling pathways that lead ...to the translocation of GLUT4. The first, and widely investigated, is the insulin activated signaling pathway through insulin receptor substrate-1 and phosphatidylinositol 3-kinase. The second is the insulin-independent signaling pathway, which is activated by contractions. Individuals with type 2 diabetes mellitus have reduced insulin-stimulated glucose uptake in skeletal muscle due to the phenomenon of insulin resistance. However, those individuals have normal glucose uptake during exercise. In this context, physical exercise is one of the most important interventions that stimulates glucose uptake by insulin-independent pathways, and the main molecules involved are adenosine monophosphate-activated protein kinase, nitric oxide, bradykinin, AKT, reactive oxygen species and calcium. In this review, our main aims were to highlight the different glucose uptake pathways and to report the effects of physical exercise, diet and drugs on their functioning. Lastly, with the better understanding of these pathways, it would be possible to assess, exactly and molecularly, the importance of physical exercise and diet on glucose homeostasis. Furthermore, it would be possible to assess the action of drugs that might optimize glucose uptake and consequently be an important step in controlling the blood glucose levels in diabetic patients, in addition to being important to clarify some pathways that justify the development of drugs capable of mimicking the contraction pathway.
Resumo Fundamentos: Poucos estudos discutiram causas para o subtratamento medicamentoso na SCA. Objetivos: Avaliar a não-administração e suspensão de medicamentos durante o tratamento ...intra-hospitalar da SCA na Estratégia de Registro de Síndrome Coronariana Aguda (estudo ERICO). Métodos: Analisamos prontuários de 563 participantes ERICO para avaliar a frequência e motivos da não administração e/ou suspensão de medicamentos. Construímos modelos de regressão logística para avaliar se sexo, idade ≥65 anos, nível educacional ou subtipo de SCA estavam associados com (a) não administração de ≥1 medicamentos; e (b) não administração ou suspensão de ≥1 medicamentos. O nível de significância foi 5%. Resultados: A amostra é composta por 58,1% de homens e com idade mediana de 62 anos. Em 183 (32,5%) participantes ≥1 medicamentos não foram administrados e 288 (51,2%) apresentaram ≥1 medicamentos não administrados ou suspensos. As causas mais frequentes foram risco de sangramento (aspirina, clopidogrel e heparina), insuficiência cardíaca (betabloqueadores) e hipotensão (inibidores da enzima conversora da angiotensina e bloqueadores dos receptores da angiotensina). Indivíduos com idade ≥65 anos (razão de chances RC:1,51; intervalo de confiança de 95% IC95%:1,05-2,19) e com angina instável (RC:1,72; IC95%:1,07-2,75) tiveram maior chance de não-administração. Considerando apenas pacientes com infarto do miocárdio, idade ≥65 anos foi associada tanto à não administração quanto à não administração ou suspensão. Conclusões: A não administração ou suspensão de ≥1 medicamento não foi rara no estudo ERICO. Indivíduos com idade ≥65 anos ou com angina instável tiveram maior chance de não administração e podem ser subtratados nesse cenário.
Naphthenic acids comprise one of the most toxic compounds of the produced water released from offshore oil platforms. Therefore, developing and applying faster, simpler, and more efficient analytical ...methods for analyzing naphthenic acids are urgently needed. Electromembrane extraction (EME) uses the electrokinetic migration of target ions through a porous membrane. Herein, the EME method was applied to extract naphthenic acids from produced water. The EME method was optimized, and the optimal conditions encompassed decanol as the organic solvent, the sample with pH 10.0, 5 min of extraction at 200 V, and the ratio 4:1 (borate buffer/matrix, v/v). Electrochemical impedance spectroscopy confirmed charged species’ migration from produced water through the EME. Subsequently, all extracts were analyzed by ultra-high-resolution mass spectrometry. The EME efficiency was assessed by comparing the extraction results to the liquid−liquid extraction (LLE) method results. Analytical results showed good linearity for both solvent and matrix curves (R 2 > 0.98). Low detection limits ranged from 0.10 to 0.13 μg mL–1 and quantification limits from 0.36 to 0.45 μg mL–1. Precision and accuracy values ranged from −13.3% to 16.5%. These values fit the proposed method, demonstrating that the EME was more efficient than LLE in naphthenic acid extraction. The EME method preferably extracted aromatic compounds with double-bond equivalence from 6 to 8. The EME coupled with ultra-high-resolution mass spectrometry was demonstrated as a promising analytical approach to naphthenic acid extraction as an efficient and more environmentally friendly alternative to conventional extraction methods.
Diffusion models have proven effective for various applications such as images, audio and graph generation. Other important applications are image super-resolution and the solution of inverse ...problems. More recently, some works have used stochastic differential equations (SDEs) to generalize diffusion models to continuous time. In this work, we introduce SDEs to generate super-resolution face images. To the best of our knowledge, this is the first time SDEs have been used for such an application. The proposed method provides an improved peak signal-to-noise ratio (PSNR), structural similarity index measure (SSIM), and consistency than the existing super-resolution methods based on diffusion models. In particular, we also assess the potential application of this method for the face recognition task. A generic facial feature extractor is used to compare the super-resolution images with the ground truth, and superior results were obtained compared with other methods. Our code is publicly available at https://github.com/marcelowds/sr-sde.
Methanol is an important product in chemical industries, having many applications: solvent, fuel and mainly being a feedstock for a large number of industrial processes. As the search for a more ...sustainable synthesis process grows, the methanol synthesis loop from synthesis gas was studied. In this work, an optimization based on an environmental objective function was developed to reduce the total water consumption of the plant. The methanol synthesis plant and the utility plants of a cooling water system and steam generation cycle were modeled and simulated. A multi-objective optimization study was performed to obtain the optimal trade-off between the economic performance and the reduced water usage. Two distinct scenarios were considered: the integrated use of the steam produced in the reactor cooling and its exporting for profits. The results show that the optimized green design can reduce up to 18% of the water consumed by the process. The exported steam case study provided more economic benefits, while the integrated steam case attained a smaller water consumption indicator.
Graphical abstract
In vivo studies show the benefits of the trypsin inhibitor isolated from tamarind (
L.) (TTI) seeds in satiety and obesity. In the present study, TTI nanoencapsulation (ECW) was performed to ...potentialize the effect of TTI and allow a controlled release in the stomach. The impact on glycemia, insulin, and lipid profile was evaluated in
rats overfed with a high glycemic index diet (HGLI). Characterization of the nanoparticles and in vitro stability in simulated gastrointestinal conditions, monitored by antitrypsin activity and HPLC, was performed. ECW and empty nanoparticles (CW) were administered by gavage, using 12.5 and 10.0 mg/kg, respectively. Both nanoformulations presented a spherical shape and smooth surface, with an average diameter of 117.4 nm (24.1) for ECW and 123.9 nm (11.3) for CW. ECW maintained the antitrypsin activity (95.5%) in the gastric phase, while TTI was completely hydrolyzed. In
rats, the nanoformulations significantly reduced glycemia and HOMA IR, and ECW increased HDL-c compared to CW (
< 0.05).Pancreas histopathology of animals treated with ECW suggested an onset of tissue repair. Thenanoencapsulation provided TTI protection, gradual release in the desired condition, and improvement of biochemical parameters related to carbohydrate metabolism disorders,without compromising insulinemia.
The third generation of advanced high-strength steels (AHSS) brought attention to the steel and automotive industries due to its good compromise between formability and production costs. This work ...evaluated a third-generation AHSS (USS CR980XG3TM) through microstructural and X-ray diffraction (XRD) analyses, uniaxial tensile and plane-strain tension testing, and numerical simulations. The damage behavior of this steel is described with the Gurson–Tvergaard–Needleman (GTN) model using an identification procedure based on the uniaxial tensile and initial microvoids data. The microstructure of the CR980XG3TM steel is composed of ferrite, martensite–austenite islands, and retained austenite with a volume fraction of 12.2%. The global formability of the CR980XG3TM steel, namely the product of the uniaxial tensile strength and total elongation values, is 24.3 GPa%. The Lankford coefficient shows a weak initial plastic anisotropy of the CR980XG3TM steel with the in-plane anisotropy close to zero (−0.079) and the normal anisotropy close to unity (0.917). The identified GTN parameters for the CR980XG3TM steel provided a good forecast for the limit strains defined according to ISO 12004-2 standard from the uniaxial tensile and plane-strain tension data.
Maple Syrup Urine Disease (MSUD) is an autosomal recessive inherited disorder caused by a deficiency in the activity of the branched-chain alpha-ketoacid dehydrogenase complex leading to the ...accumulation of branched-chain amino acids (BCAA) leucine, isoleucine, and valine and their respective branched-chain α-ketoacids and corresponding hydroxy acids. Considering that
Danio rerio
, known as zebrafish, has been widely used as an experimental model in several research areas because it has favorable characteristics that complement other experimental models, this study aimed to evaluate oxidative stress parameters in zebrafish exposed to high levels of leucine (2 mM and 5 mM), in a model similar of MSUD. Twenty-four hours after exposure, the animals were euthanized, and the brain content dissected for analysis of oxidative stress parameters: thiobarbituric acid reactive substances (TBARS), 2′,7′-dichlorofluorescein oxidation assay (DCF); content of sulfhydryl, and superoxide dismutase (SOD) and catalase (CAT) activities. Animals exposed to 2 mM and 5 mM leucine showed an increase in the measurement of TBARS and decreased sulfhydryl content. There were no significant changes in DCF oxidation. In addition, animals exposed to 2 mM and 5 mM leucine were found to have decreased SOD activity and increased CAT activity. Based on these results, exposure of zebrafish to high doses of leucine can act as a promising animal model for MSUD, providing a better understanding of the toxicity profile of leucine exposure and its use in future investigations and strategies related to the pathophysiology of MSUD.
Naturalistic studies evaluate individuals in their usual way of living, presenting more "real-life" data regarding patients and their diseases.
To investigate demographic, clinical, and genetic ...factors that could be predictive of good response to cholinesterase inhibitors (ChEI) treatment in Alzheimer's disease (AD) and AD + cerebrovascular disease (CVD).
A total of 129 patients were diagnosed with AD or AD + CVD and with mild-to-moderate dementia. After a 12-month treatment, 97 patients completed the study. They were evaluated at baseline and after three, six, and 12 months of ChEI (donepezil or rivastigmine or galantamine) use. APOE genotype and CYP2D6 polymorphisms were determined for all of the participants. In each visit, we used cognitive, functional, mood, and behavior scales. We classified patients according to their scores in the Mini-Mental State Examination (MMSE). Good responders were defined as those scoring ≥2 in the MMSE at 12 months.
The rate of good clinical response was 27.8%. In a longitudinal analysis, the patients with mild AD and also good responders at three months were considered to be good responders at 12 months. There was no correlation between ChEI dose, APOE and CYP2D6 polymorphisms, and the pattern of clinical response.
A higher rate of good response was observed in this study compared to that in previous investigations. The pharmacogenetic aspects do not seem to have an influence in the response.
PDF
