Melatonin Inhibits Benzene-Induced Lipid Peroxidation in Rat Liver Sharma, Seema (Toxicology Laboratory, Department of Zoology, Ch. Charan Singh University, Meerut, India); Rana, Suresh (Toxicology Laboratory, Department of Zoology, Ch. Charan Singh University, Meerut, India)
Arhiv za higijenu rada i toksikologiju,
03/2010, Volume:
61, Issue:
1
Journal Article
Peer reviewed
Open access
We studied the antioxidative role of melatonin against benzene toxicity in rat liver. The inhibition of mitochondrial and microsomal lipid peroxidation differed between 24-hour (single-dose), 15-day, ...and 30-day treatments. Inhibition of mitochondrial lipid peroxidation was the highest after the single dose of melatonin, whereas highest microsomal inhibition was recorded after 30 days of melatonin treatment. No significant difference was recorded between 15-day and 30-day treatments. Cytochrome P 450 2E1 (CYP 450 2E1) activity declined after the single-dose and 15-day melatonin treatment in the benzene-treated group, but it rose again, though not significantly after 30 days of treatment. Liver histopathology generally supported these findings. Phenol concentration in the urine samples declined in melatonin and benzene-treated rats. Our results show that melatonin affects CYP 450 2E1, which is responsible for benzene metabolism. Inhibition of its metabolism correlated with lower lipid peroxidation. In conclusion, melatonin was found to be protective against lipid peroxidation induced by benzene.
Istražena je antioksidacijska uloga melatonina u zaštiti protiv toksi _noga djelovanja benzena u jetri štakora. Utvrđeno je da kratkoro _no odnosno dugoro _nije lije _enje štakora melatoninom u razli _itoj mjeri štiti štakore istodobno izložene benzenu. Inhibicija lipidne peroksidacije mitohondrija i mikrosoma bila je razli _ita nakon 24 h, 15 dana, odnosno 30 dana lije _enja melatoninom. Najveća inhibicija lipidne peroksidacije mitohondrija zamijećena je nakon primjene jednokratne doze melatonina, dok je najizraženija inhibicija u mikrosomima zamijećena nakon 30 dana lije _enja melatoninom. Sli _na istraživanja pokazuju da razina glutationa (GSH) najviše raste nakon 24 h lije _enja melatoninom. Nije zamijećena razlika između lije _enja u trajanju od 15 odnosno 30 dana. U štakora koji su uz benzen istodobno primali i melatonin razine citokroma P 450 2E1 pale su nakon 24 h odnosno 15 dana izloženosti. U štakora koji su primali samo melatonin te su razine nakon 30 dana statisti _ki nezna _ajno porasle u odnosu na skupinu izloženu samo benzenu. Histopatološka analiza jetre na _elno je potvrdila ove nalaze. Koncentracije fenola u mokraći bile su niže u štakora koji su istodobno primali melatonin i benzen. Ovi rezultati pokazuju da melatonin utje _e na citokrom P 450 2E1, koji je odgovoran za metabolizam benzena. Inhibira li se njegov metabolizam, smanjuje se lipidna peroksidacija. Zaklju _ak je da melatonin štiti od lipidne peroksidacije uzrokovane benzenom.
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