It has been reported that tetraspanin CD151 acts as a promoter of metastasis in several tumors and plays an important role in c‐Met/hepatocyte growth factor signaling. However, the role of CD151 ...alone and coexpression of CD151/c‐Met in hepatocellular carcinoma (HCC) remains unclear. We found that expression of CD151 was positively related to metastatic potential of HCC cell lines, and modified cells with CD151high showed higher secretion of matrix metalloproteinase 9 and aggressiveness in vitro and higher metastatic ability in vivo. Furthermore, HCC patients with vascular invasion, large tumors, multiple tumors, high tumor‐node‐metastasis stage, and undifferentiated tumor were prone to have higher CD151 expression. The postoperative 3‐, 5‐, and 7‐year overall survival (OS) of patients in HCCs with CD151high were significantly lower than those in the CD151low group, and correspondingly cumulative recurrence rates in HCCs with CD151high were significantly higher than those in the CD151low group. Both CD151 and c‐Met were remarkably overexpressed in HCCs, compared with adjacent nontumorous and normal liver tissues. Pearson correlation analysis showed a slight correlation between CD151 and c‐Met in HCCs. Importantly, the 5‐ and 7‐year OS rates in CD151high/c‐Methigh patients were 50.5% and 37.8%, respectively, significantly lower than those of CD151low/c‐Metlow patients (63.9% and 54.6%, respectively). Five‐ and 7‐year cumulative recurrence rates in CD151high/c‐Methigh patients were 53.3% and 71.9%, respectively, markedly higher than those of CD151low/c‐Metlow patients (39.0% and 52.5%, respectively). Multivariate analysis revealed that CD151 and combination of CD151/c‐Met were independent prognostic indicators for OS and cumulative recurrence. Conclusion: CD151 is positively associated with invasiveness of HCC, and CD151 or combination of CD151/c‐Met is a novel marker in predicting the prognosis of HCC and a potential therapeutic target. (HEPATOLOGY 2008.)
Background
Inflammatory reactions at a tumor site have both detrimental and beneficial effects on tumor progression. This study was designed to assess the clinical significance of tumor-infiltrating ...inflammatory cells in patients with intrahepatic cholangiocarcinoma (ICC).
Methods
A total of 123 consecutive ICC patients who underwent curative resection were enrolled. Tissue microarray and immunohistochemistry were used to analyze the distribution and clinical relevance of IL-17
+
, FOXP3
+
, CD8
+
, CD66b
+
cells, and microvessel density (CD34) in different microanatomical areas.
Results
IL-17
+
cells, FOXP3
+
lymphocytes, CD66b
+
neutrophils, and microvessels were enriched predominantly in intratumor (IT) area, whereas CD8
+
lymphocytes were most abundant in tumor invasive front. On univariate analyses, increasing IL-17
IT
+
and neutrophils
IT
were significantly associated with worse patient survival. Multivariate analyses revealed that IL-17
IT
+
(hazard ratio HR = 1.59; 95% confidence interval CI, 1.05–2.41;
P
=
0.028), neutrophils
IT
(HR = 1.76; 95% CI, 1.16–2.65;
P
=
0
.007), and their combination (HR 2.8; 95% CI 1.72–4.57;
P
< 0.001) were independent prognostic factors, which were superior to conventional clinicopathologic features, such as intrahepatic metastasis and TNM stage. IL-17
IT
+
significantly correlated with the presence of lymph node metastasis, intrahepatic metastasis, and advanced stages, whereas neutrophils
IT
correlated with the presence of vascular invasion. In addition, significant positive correlations were detected among densities of IL-17
+
cells, neutrophils, and microvessel density.
Conclusions
Our data suggested that intratumor IL-17
+
cells, neutrophils are novel, powerful predictors of prognosis in patients with ICC.
Reaction processes, powder characteristics, sinterability, and mechanical properties of TiB2 prepared by borothermal reduction, B4C reduction, and boro/carbothermal reduction were compared. Results ...showed that TiBO3 and Ti2O3 as the intermediate phases existed in the three reduction processes. The temperature where TiB2 became major phase was lowest during borothermal reduction, resulting in the finest TiB2 particle size, in contrast to the other two methods, especially boro/carbothermal reduction. However, TiB2 powders prepared by B4C reduction and boro/carbothermal reduction after pressureless sintering at 1800°C for 2 hours showed the relatively higher sinterability, due to the lower oxygen content and higher carbon content. Finally, using 5 wt% Ni as sintering additive, hot‐pressed TiB2 ceramics from B4C reduction demonstrated higher densification, more fine‐grained microstructure and higher mechanical properties, due to the better balance of oxygen/carbon content.
Color-tunable room-temperature phosphorescence (RTP) with potential in many fields is of great importance but extremely challenging. It is necessary to comprehend the correlation between the ...molecular structure and property to design and synthesize such materials. Metal–organic coordination polymers (CPs) with good predesignability and precise structure have become a platform to construct RTP materials. Herein, three zinc-based CPs containing halogen and a flexible tetradentate ligand are synthesized. All of these CPs present two constant emission regions and an excitation-dependent emission region. Structure–property analysis shows that these emissions originate from isolated chromophores and dimerized chromophores as well as various charge transfers. The phosphorescence colors of these CPs can be modulated by excitation and temperature. This study provides a novel strategy to construct multicolor and multiresponsive RTP materials based on metal–organic coordination polymers.
Background： Nontraumatic osteonecrosis of the femoral head （NONFH） is a debilitating disease that represents a significant financial burden for both individuals and healthcare systems. Despite its ...significance, however, its prevalence in the Chinese general population remains unknown. This study aimed to investigate the prevalence of NONFH and its associated risk factors in the Chinese population. Methods： A nationally representative survey of 30,030 respondents was undertaken from June 2012 to August 2013. All participants underwent a questionnaire investigation, physical examination of hip, and bilateral hip joint X-ray and/or magnetic resonance imaging exalnination. Blood samples were taken after overnight fasting to test serum total cholesterol, triglyceride, and high-density lipoprotein （HDL） and low-density lipoprotein （LDL） levels. We then used multivariate logistic regression analysis to investigate the associations between various metabolic, demographic, and lifestyle-related variables and NONFH. Results： NONFH was diagnosed in 218 subjects （0.725%） and the estimated NONFH cases were 8.12 million among Chinesepeople aged 15 years and over. The prevalence of NONFH was significantly higher in males than in females （1.02% vs. 0.51%, x^2 = 24.997, P 〈 0.001 ）. Among NONFH patients, North residents were subjected to higher prevalence of NONFH than that of South residents （0.85% vs. 0.61%,x^2= 5.847, P = 0.016）. Our multivariate regression analysis showed that high blood levels oftriglycerides, total cholesterol, LDL-cholesterol, and non-H DL-cholesterol, male, urban residence, family history of osteonecrosis of the femoral head. heavy smoking, alcohol abuse and glucocorticoid intake, overweight, and obesity were all significantly associated with an increased risk of NONFH. Conclusions： Our findings highlight that NONFH is a significantpublic health challenge in China and underscore the need for policy measures on the national level. Furthermore, NONFH shares a number of risk factors with atherosclerosis.
: PD1/PD-L1 immune checkpoint inhibitors have shown promising results for several malignancies. However, PD1/PD-L1 signaling and its therapeutic significance remains largely unknown in intrahepatic ...cholangiocarcinoma (ICC) cases with complex etiology.
: We investigated the expression and clinical significance of CD3 and PD1/PD-L1 in 320 ICC patients with different risk factors. In addition, we retrospectively analyzed 7 advanced ICC patients who were treated with PD1 inhibitor.
: The cohort comprised 233 patients with HBV infection, 18 patients with hepatolithiasis, and 76 patients with undetermined risk factors. PD-L1 was mainly expressed in tumor cells, while CD3 and PD1 were expressed in infiltrating lymphocytes of tumor tissues. PD1/PD-L1 signals were activated in tumor tissues, and expression was positively correlated with HBV infection and lymph node invasion. More PD1
T cells and higher PD-L1 expression were observed in tumor tissues of ICC patients with HBV infection compared to patients with hepatolithiasis or undetermined risk factors. More PD1
T cells and/or high PD-L1 expression negatively impacted the prognosis of patients with HBV infection but not those with hepatolithiasis. Multivariate analysis showed PD1/PD-L1 expression was an independent indicator of ICC patient prognosis. Advanced ICC patients with HBV infection and less PD1
T cells tended to have good response to anti-PD1 therapy.
: Hyperactivated PD1/PD-L1 signals in tumor tissues are a negative prognostic marker for ICCs after resection. HBV infection- and hepatolithiasis-related ICCs have distinct PD1/PD-L1 profiles. Further, PD1
T cells could be used as a biomarker to predict prognosis and assay the efficiency of anti-PD1 immunotherapy in ICC patients with HBV infection.
Pumped storage power plants (PSPPs) are among the most efficient and practical large-scale energy storage systems. In recent years, several transformer failures have occurred in PSPPs, and transient ...overvoltage is considered a potential reason for failure. However, there is a lack of field measurement results for transient analysis. In this paper, a switching transient measurement system and multipoint synchronous triggering system were developed. Field measurements were carried out in three PSPPs. According to the measurement results, the maximum amplitude of the switching overvoltage was 630.6±46.5 kV. The shortest front time was 76±7.6 ns. The largest steepness was 1130±140.8 kV/μs. A simulation model was built to analyze three phenomena in the transient, including multistep waveform, overvoltage peak, and power transformer demagnetization. The measurement and analysis results are beneficial for the improvement of simulation modeling. It is helpful to pinpoint potentially damaging transients for equipment manufacturers and designers of PSPPs.
China is the country in the world with the most abundant magnesite resources. The ever-increasing deposit of low grade magnesite ore derived from the process of magnesium products has occupied a ...substantial space and caused environmental damages. In this study, crushed low grade magnesite (CLGM) was prepared and then applied in mortar as fine aggregate to partially or entirely replace natural river sand (30%, 60%, and 100%), while 100% limestone sand (LSS) was applied as the reference group. The findings of this study demonstrated a positive correlation between the ratio of CLGM substitution and the improvement of both engineering properties and sustainable potential in the mortar. Compared with the control group at 28 d, the 100% CLGM mortar exhibited improvements of up to 6% in splitting strength, 25.86% in compressive strength, 16% in porosity reduction, 22.22% in impermeability enhancement, 27.52% in resistance to chloride ion penetration, 21.66% in frost resistance (after 150 cycles), and a remarkable 54.8% in anti-abrasion property. Furthermore, the sustainability evaluation assessed its potential societal value, and highlighted its reduced production costs (down 52.28%) and impressive environmental benefits (notable decreases of 28.3% in ozone depletion potential, 33.2% in land use potential, 38.7% in marine ecotoxicity, and 33.9% in fossil resource scarcity). These findings well demonstrated the feasibility of adopting magnesite tailings as a substitute of conventional aggregate within the construction sector along with substantial economic savings and positive environmental ramifications. The successful application of CLGM mortar in cleaner production can further facilitate the sustainable advancement of environment and construction industry.
•Magnesite tailing is first investigated as river sand substitution in mortar.•The 100% CLGM mortar can exhibit better mechanical and durability properties.•The higher the ratio of CLGM, the denser the structure due to the filler effect.•The specific environmental impacts were quantified through the LCA approach.•The recycling of CLGM has great economic, social and environmental benefits.
This work investigated the effect of ZrB2 content on phase composition, relative density, microstructure, and mechanical performance of Si3N4 ceramics prepared by Si nitridation and hot‐pressing ...method. Si3N4 ceramics were fabricated using Si powder by nitridation and hot‐pressing process. Results showed that the addition of ZrB2 enhanced the transformation of α‐Si3N4 into β‐Si3N4 phase during nitridation, resulting in a greater fraction of β‐Si3N4 (above 90 wt.%) phase in Si3N4 ceramics with ZrB2 after hot‐pressing. In contrast, ceramics without ZrB2 had a lower ratio of β‐Si3N4 (40.4 wt.%). The use of 2.5 vol.% ZrB2 yielded a significant bimodal microstructure and increased fracture toughness to 5.0 ± 0.2 MPa·m1/2, but decreased Vickers hardness to 16.4 ± 0.5 GPa. However, increasing the ZrB2 content to 5 vol.% and 10 vol.% led to a massive formation of equiaxed coarse β‐Si3N4 grains and a significant decrease in Vickers hardness and fracture toughness.
The aqueous reprocessing of spent nuclear fuel generates a considerable amount of plutonium alongside contaminated stainless steel, necessitating meticulous handling for safe decommission and ...long‐term management. The present work investigated the co‐immobilization of CeO2 (as an inactive surrogate for PuO2) with metallic Fe and Cr (representing a simplified stainless steel) within a zirconolite ceramic wasteform, nominally targeting Ca1−xCexZrTi2−2xFexCrxO7. After sintering at 1400°C under an air atmosphere, the zirconolite phase constituted 90.8–95.1 wt.% of the product across the solid solution range of 0.05 ≤ x ≤ 0.25, alongside perovskite and baddeleyite secondary phases in varying proportion; no evidence of the unincorporated CeO2 or metallic and oxidized Fe or Cr were identified. Above x = 0.30 CeO2 was detected inferring, the solubility limit was reached. A polytype transformation from zirconolite 2M to 3T was confirmed by X‐ray diffraction and selected area electron diffraction results, with the relative fraction of the 3T phase gradually increasing to 49.5 wt.% at x = 0.30. Deconvolution of X‐ray photoelectron spectroscopy data revealed the partial reduction of Ce4+–Ce3+, whereas Fe and Cr species maintained trivalent, in agreement with the targeted substitution scheme. Benefitting from the excellent chemical flexibility of zirconolite structured compounds, the co‐immobilization approach may be an effective disposal pathway for Pu‐containing wastes and contaminated stainless steel residues.
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