Background
The bitter gourd fruit yields are not at a desirable level due to maleness. Maleness is one of the biggest difficulties with bitter gourd, reducing fruit yields substantially. On the other ...hand, plant stages are the most important consideration for PGR application because of their sensitive responsiveness. Therefore, it is necessary to determine how bitter gourd plants respond to plant growth regulators, namely PGR
0
(control: application of water as control treatment), PGR
1
(GA
3
-Gibberellic acid, 100 mgL
−1
), PGR
2
(NAA-Naphthalene acetic acid, 100 mgL
−1
), and PGR
3
(MH- Maleic hydrazide, 100 mgL
−1
) at three application phases, S
1
: soaking of seeds; S
2
: four-leaf; and S
3
: flower bud in terms of vegetative development, male–female flower sex alteration, and fruit features including bioactive compounds.
Results
Physiological, floral and yield characteristics of bitter gourd exposed significant changes by GA
3
and MH at various stages. The number of leaves plant
−1
, the fresh mass of the plant, and the fresh mass of fruit and fruit dry matter content were enhanced remarkably for MH application at the seed soaking stage except for plant height and the number of branches plant
−1
. MH increased the number of female flowers plant
−1
, the number of fruits plant
−1
and the yield at the 4-leaf stage of application. PGRs substantially influenced the quantity of sugar, chlorophyll, total carotenoids, protein, and water content at various phases of their application but not on ascorbic acid, TSS, or total phenol concentration.
Conclusions
For sex expression and yield attributes, 100 mgL
−1
MH at the 4-leaf stage would be effective in bitter gourd. Exogenous NAA showed a considerable dual influence on plant development and antioxidant enzyme activity in bitter gourd fruits.
Tomato (
L.) is a popular nutritious vegetable crop grown in Malaysia and other parts of the world. However, fungal diseases such as anthracnose pose significant threats to tomato production by ...reducing the fruit quality and food value of tomato, resulting in lower market prices of the crop globally. In the present study, the etiology of tomato anthracnose was investigated in commercial tomato farms in Sabah, Malaysia. A total of 22 fungal isolates were obtained from anthracnosed tomato fruits and identified as
species, using morphological characteristics. The phylogenetic relationships of multiple gene sequence alignments such as internal transcribed spacer (ITS), β-tubulin (
), glyceraldehyde 3-phosphate dehydrogenase (
), actin (
), and calmodulin (
), were adopted to accurately identify the
species as
. The results of pathogenicity tests revealed that all
isolates caused anthracnose disease symptoms on inoculated tomato fruits. To our knowledge, the present study is the first report of tomato anthracnose caused by
in Malaysia. The findings of this study will be helpful in disease monitoring, and the development of strategies for effective control of anthracnose on tomato fruits.
The production of horticultural crops in the outdoor environment facing various environmental factors, such as cyclones, droughts, heavy rain, and hailstorms, significantly affects the papaya ...production in the sub-tropical regions, especially in Bangladesh. Protected cultivation of horticultural crops is a common practice in developed countries. However, it is rarely observed in the developing countries, particularly for papaya production. Therefore, this study was carried out to find a protective approach to papaya cultivation to mitigate the environmental factors to obtain a quality yield. This production system consists of three treatments, including net house, poly shed house, UV poly shed house, and open field conditions (control). The results revealed that plants grown in the net house had significantly higher leaf number (30), fruit number (68), and fruit yield (56.28 kg/plant) than the control grown plant. Papaya cultured in the net house also showed significantly higher accumulation of chlorophyll, ascorbic acid, total phenol, reducing sugar, and β-carotene than those grown in other environments. In terms of peel color, papaya grown in the net house had the highest a* value (redness), whereas that grown in the open field had the lowest. Thus, the study demonstrated that papaya can be cultivated successfully in a net house with increased yield and phytochemical content. The findings provide a fundamental production strategy for quality papaya production in Bangladesh.
The presented study established Agrobacterium-mediated genetic transformation using protocorm-like bodies (PLBs) for the production of transgenic Vanda Kasem's Delight Tom Boykin (VKD) orchid. ...Several parameters such as PLB size, immersion period, level of wounding, Agrobacterium density, cocultivation period, and concentration of acetosyringone were tested and quantified using gusA gene expression to optimize the efficiency of Agrobacterium-mediated genetic transformation of VKD's PLBs. Based on the results, 3-4 mm PLBs wounded by scalpel and immersed for 30 minutes in Agrobacterium suspension of 0.8 unit at A 600 nm produced the highest GUS expression. Furthermore, cocultivating infected PLBs for 4 days in the dark on Vacin and Went cocultivation medium containing 200 𝜇M acetosyringone enhanced the GUS expression. PCR analysis of the putative transformants selected in the presence of 250 mg/L cefotaxime and 30 mg/L geneticin proved the presence of wheatwin1, wheatwin2, and nptII genes.
Protecting food crops from viral pathogens is a significant challenge for agriculture. An integral approach to genome-editing, known as CRISPR/Cas9 (clustered regularly interspaced short palindromic ...repeats and CRISPR associated protein 9), is used to produce virus-resistant cultivars. The CRISPR/Cas9 tool is an essential part of modern plant breeding due to its attractive features. Advances in plant breeding programs due to the incorporation of Cas9 have enabled the development of cultivars with heritable resistance to plant viruses. The resistance to viral DNA and RNA is generally provided using the Cas9 endonuclease and sgRNAs (single-guide RNAs) complex, targeting particular virus and host plant genomes by interrupting the viral cleavage or altering the plant host genome, thus reducing the replication ability of the virus. In this review, the CRISPR/Cas9 system and its application to staple food crops resistance against several destructive plant viruses are briefly described. We outline the key findings of recent Cas9 applications, including enhanced virus resistance, genetic mechanisms, research strategies, and challenges in economically important and globally cultivated food crop species. The research outcome of this emerging molecular technology can extend the development of agriculture and food security. We also describe the information gaps and address the unanswered concerns relating to plant viral resistance mediated by CRISPR/Cas9.
The fungal species belonging to the genus Trichoderma has been globally recognized as a potential candidate of biofertilizer and biocontrol agent to prevent devastating soil-borne fungal pathogens ...and enhance growth and productivity of agricultural crops. The antagonistic activity of Trichoderma to pathogenic fungi is attributed to several mechanisms including antibiosis and enzymatic hydrolysis, which are largely associated with a wide range of metabolites secreted by the Trichoderma species. Besides suppressing target pathogens, several metabolites produced by Trichoderma species may act against non-pathogenic beneficial soil microbial communities and perform unintended alterations within the structures and functions of microbial communities in the crop rhizosphere. Multiple microbial interactions have been shown to enhance biocontrol efficacy in many cases as compared to bioinoculant employed alone. The key advances in understanding the ecological functions of the Trichoderma species with special emphasis on their associations with plant roots and other microbes exist in the crop rhizosphere, which are briefly described here. This review focuses on the interactions of metabolites secreted by Trichoderma species and plant roots in the rhizosphere and their impacts on pathogenic and non-pathogenic soil microbial communities. The complex interactions among Trichoderma–plants–microbes that may occur in the crop rhizosphere are underlined and several prospective avenues for future research in this area are briefly explored. The data presented here will stipulate future research on sustainably maximizing the efficiency of Trichoderma inoculation and their secondary metabolites in the crop soil ecosystem.
Seed priming is a technique that can potentially facilitate rapid and consistent germination and subsequent plant growth. The present study investigates the effect of different seed priming ...treatments and processing times on germination and growth efficiency for the effective cultivation of Yard-long bean. Thirteen different primings were used to determine the stimulatory effect on the germination and yield performance of the Yard-long bean. The priming treatments included control (without priming); hydro priming for 12, 18, 24, and 30 h; halo priming (1% CaCl2) for 12, 18, 24, and 30 h; and halo priming (2% KNO3) for 12, 18, 24, and 30 h. Studies showing the highest level of germination (86.66%), germination index (35.69), seedling vigor index (1833.80), number of branches (7.20), and pod yields per plant (1836.00 g) were recorded from halo priming with 1% CaCl2 at 12 h treatment. Halo priming with 1% CaCl2 at 12 h is thus considered to be a compatible priming technique for the germination of seeds and a higher yield of Yard-long bean.
Different designs of the plant tissue culture vessel, such as size, material, and shape, may alter its microenvironment atmosphere. The present study was conducted on protocorm-like bodies (PLBs) of
...Sabin Blue orchid to determine the development of PLBs on plastic and glass culture vessels of different sizes. PLBs were cultured in half-strength Murashige and Skoog (MS) medium with the same initial weight of 0.5 g in 10 replicates. The growth index of the PLBs was calculated after 11 weeks to study their growth in every vessel; additionally, biochemical analysis was performed to determine carbohydrate content, proline concentration, and photosynthesis pigments in the PLBs. Scanning electron microscopy (SEM) was performed to study stomata development on PLBs in each vessel, and histological analyses were conducted to study the cell structure. Overall, the PLBs cultured in a large 470 ml plastic vessel showed successful growth with a high growth index, high carbohydrate content, low-stress condition, and high chlorophyll content. SEM confirmed that the presence of trichome and rhizoid in PLBs cultured in the 470 ml plastic vessel. Histological analysis showed the formation of the shoot on the PLBs and the presence of starch granules. Thus, the use of plastic as a culture vessel provides a good impact for culturing PLBs and has low cost.
Irrational applications of insecticides on vegetable crops are very common in Bangladesh, resulting in harmful consequences for the environment and human health. Therefore, a study was conducted to ...evaluate the efficacy of biological and botanical insecticides on okra shoot and fruit borer grown in open fields. Four insecticides were used in this study, namely Bacillus thuringiensis (Bt), Spinosad 45 SC, Abamectin 1.8 EC, and Azadirachtin 1% EC an untreated control. The experiment used a randomized complete block design (RCBD) with three replications. The results showed that the rate of shoot infestation was the lowest in the Spinosad-treated plot (3.80%), and the highest was in the control (20.67%). The lowest fruit infestation (3.56%) was recorded in the treated plot of Spinosad. The rate of reduction of fruit infestation over control was 80.69, 60.14, 56.45, and 55.58% in the plots treated with Spinosad, Bt, Azadirachtin, and Abamectin, respectively. Consequently, the Spinosad-treated plot attained the highest yield (8.65 t ha−1), which was followed by the plots treated with Azadirachtin (6.74 t ha−1), Bt (6.28 t ha−1), and Abamectin (6.12 t ha−1). The highest net return and benefit–cost ratio (BCR) were 542.36 US$ and 2.64, obtained respectively from the Spinosad-treated plot. The second highest BCR (1.70) was obtained from the Azadiratin-treated plot, and the lowest BCR (1.18) was recorded in the Abamectin-treated plot. Therefore, the studied insect management practices could be incorporated to attain higher yields and economic benefits for growing okra in Bangladesh.
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