A new experimental investigation of decaying turbulence generated by a low-blockage space-filling fractal square grid is presented. We find agreement with previous works by Seoud & Vassilicos (Phys. ...Fluids, vol. 19, 2007, 105108) and Mazellier & Vassilicos (Phys. Fluids, vol. 22, 2010, 075101) but also extend the length of the assessed decay region and consolidate the results by repeating the experiments with different probes of increased spatial resolution. It is confirmed that this moderately high Reynolds number ${\mathit{Re}}_{\lambda } $ turbulence (up to ${\mathit{Re}}_{\lambda } \simeq 350$ here) does not follow the classical high Reynolds number scaling of the dissipation rate $\varepsilon \ensuremath{\sim} {u{}^{\ensuremath{\prime} } }^{3} / L$ and does not obey the equivalent proportionality between the Taylor-based Reynolds number ${\mathit{Re}}_{\lambda } $ and the ratio of integral scale $L$ to the Taylor microscale $\lambda $. Instead we observe an approximate proportionality between $L$ and $\lambda $ during decay. This non-classical behaviour is investigated by studying how the energy spectra evolve during decay and examining how well they can be described by self-preserving single-length-scale forms. A detailed study of homogeneity and isotropy is also presented which reveals the presence of transverse energy transport and pressure transport in the part of the turbulence decay region where we take data (even though previous studies found mean flow and turbulence intensity profiles to be approximately homogeneous in much of the decay region). The exceptionally fast turbulence decay observed in the part of the decay region where we take data is consistent with the non-classical behaviour of the dissipation rate. Measurements with a regular square mesh grid as well as comparisons with active-grid experiments by Mydlarski & Warhaft (J. Fluid Mech., vol. 320, 1996, pp. 331–368) and Kang, Chester & Meveneau (J. Fluid Mech., vol. 480, 2003, pp. 129–160) are also presented to highlight the similarities and differences between these turbulent flows and the turbulence generated by our fractal square grid.
The substitution of fish resources as ingredients for aquafeeds by those based on vegetable sources is needed to ensure aquaculture sustainability in the future. It is known that Senegalese sole ...(Solea senegalensis) accepts high dietary content of plant ingredients without altering growth or flesh quality parameters. However, scarce information is available regarding the long-term impact of vegetable diets (combining the inclusion of both vegetable protein and oils) on the stress response and immunity of this fish species. This study aims to evaluate the concomitant effect of the extended use of vegetable protein-based diets with fish oil (FO) replacement (0, 50 or 100%) by vegetable oils (VO), on the response to acute (10 min) or prolonged (4 days) stress, induced by thermal shock. Plasma levels of cortisol, glucose and lactate as well as hepatic levels of glucose, glycogen and lactate were evaluated as primary and secondary responses to stress, 6 and 18 months after feeding the experimental diets (6 and 18 MAF). The brain monoaminergic activity in telencephalon and hypothalamus, and non-specific immune parameters were also evaluated. As expected, thermal shock induced an increase in values of plasma parameters related to stress, which was more evident in acute than in prolonged stress. Stress also affected lactate levels in the liver and the values of the alternative complement pathway-ACH50 in the plasma. Dietary substitution of FO induced an effect per se on some parameters such as decreased hepatic glucose and glycogen levels and peroxidase activity in plasma as well enhanced serotonergic activity in brain of non-stressed fish. The results obtained in some parameters indicate that there is an interaction between the use of vegetable diets with the physiological response to thermal stress, as is the case of the hepatic lactate, serotonergic neurotransmission in brain, and the activity of ACH50 in plasma. These results suggest that the inclusion of VO in plant protein based diets point to a slightly inhibited stress response, more evident for an acute than a prolonged stress.
Direct numerical simulations of statistically steady homogeneous isotropic turbulence in viscoelastic fluids described by the FENE-P model, such as those laden with polymers, are presented. It is ...shown that the strong depletion of the turbulence dissipation reported by previous authors does not necessarily imply a depletion of the nonlinear energy cascade. However, for large relaxation times, of the order of the eddy turnover time, the polymers remove more energy from the large scales than they can dissipate and transfer the excess energy back into the turbulent dissipative scales. This is effectively a polymer-induced kinetic energy cascade which competes with the nonlinear energy cascade of the turbulence leading to its depletion. It is also shown that the total energy flux to the small scales from both cascade mechanisms remains approximately the same fraction of the kinetic energy over the turnover time as the nonlinear energy cascade flux in Newtonian turbulence.
A novel mechanism to produce and detect light dark matter in experiments making use of GeV electrons (and positrons) impinging on a thick target (beam dump) is proposed. The positron-rich environment ...produced by the electromagnetic shower allows us to produce an A^{'} via nonresonant (e^{+}+e^{-}→γ+A^{'}) and resonant (e^{+}+e^{-}→A^{'}) annihilation on atomic electrons. The latter mechanism, for some selected kinematics, results in a larger sensitivity with respect to limits derived by the commonly used A^{'}-strahlung. This idea, applied to beam-dump experiments and active beam-dump experiments, pushes down the current limits by an order of magnitude.
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The previously reported non-equilibrium dissipation law is investigated in turbulent flows generated by various regular and fractal square grids. The flows are documented in terms of various ...turbulent profiles which reveal their differences. In spite of significant inhomogeneity and anisotropy differences, the new non-equilibrium dissipation law is observed in all of these flows. Various transverse and longitudinal integral scales are measured and used to define the dissipation coefficient
$C_{\varepsilon }$
. It is found that the new non-equilibrium dissipation law is not an artefact of a particular choice of the integral scale and that the usual equilibrium dissipation law can actually coexist with the non-equilibrium law in different regions of the same flow.
Over the last few years, several aspects of Senegalese sole (Solea senegalensis) culture have been developed and optimised but the dietary lipid level for optimal growth has never been determined. ...Hence, five isonitrogenous diets (56 % dietary protein) with increasing dietary lipid levels (4, 8, 12, 16 and 20 % DM) were fed to satiation to triplicate groups of twenty fish (mean initial weight 10 g). Fifteen tanks were randomly assigned one of the five diets. Feed was distributed using automatic feeders, and fish were fed over a 16-week period. At the end of the experiment the fish fed on diets containing the two lowest dietary lipid levels (4 and 8 %) showed a 3-fold body-weight increase with a significantly higher daily growth index than fish fed higher lipid levels (1·2 v. 0·8). Moreover, these fish displayed a significantly lower dry feed intake (12 g/kg per d) and feed conversion ratio (1·0) compared with fish fed higher lipids levels (16–19 g/kg per d; feed conversion ratio 2·0). Low dietary lipid levels ( < 12 %) significantly improved nutrient retention and gain and hence growth, without major effects on whole-body composition. Despite the slight alteration in n-3 PUFA muscle content in the fish fed low-fat-diets, this fish fed low dietary lipid still remains a rich n-3 PUFA product and generally maintained its nutritional value. These results evidenced a low lipid tolerance of Senegalese sole juveniles and suggest a maximal dietary inclusion level of 8 % lipids for both optimal growth and nutrient utilisation without compromising flesh quality.
Dietary inclusion of IMTA-cultivated
Gracilaria vermiculophylla
was evaluated in rainbow trout. Growth and feed efficiency were determined in fish fed 0 % (CTRL), 5 % (G5), and 10 % (G10) of the red ...seaweed for 91 days. Carotenoid concentration (skin and muscle), immunological parameters, and intestinal morphology were also evaluated. G10 group showed the lowest final body weight, with feed and protein efficiency ratios being significantly lower than the CTRL. Although protein intake was similar among groups, G10 diet induced the lowest protein retention and gain probably due to its smallest intestine diameter and lowest
villi
height. Fish fed G10 diet displayed higher carotenoid content in the skin (16.7 μg g
−1
) when compared with the CTRL group, but a lower concentration was registered in the flesh (0.23 μg g
−1
). Instrumental color showed that fillets were more luminous (
L
*), less yellowish (
b
*), and more reddish (a*) with seaweed inclusion and the lowest chrome intensity (
C
*) in the G10 group confirmed the lowest muscle carotenoid content in these fish. G5 diet enhanced the innate immune response of rainbow trout inducing the highest peroxidase, alternative complement (ACH50), and lysozyme activities. The inclusion of
Gracilaria
meal in diets for rainbow trout is possible up to 5 %, but a higher inclusion level impairs growth.
High levels of dietary lipids are incorporated in feeds for most teleost fish to promote growth and reduce nitrogen waste. However, in Senegalese sole (Solea senegalensis) previous studies revealed ...that increasing the level of dietary lipids above 8% negatively affect growth and nutrient utilization regardless of dietary protein content. It has been shown that glucose regulation and metabolism can be impaired by high dietary fat intake in mammals, but information in teleost fish is scarce. The aim of this study was to assess the possible effect of dietary lipids on glucose metabolism in Senegalese sole with special emphasis on the regulation of proteins involved in the muscle insulin-signalling pathway. Senegalese sole juveniles (29 g) were fed two isonitrogenous diets (53% dry matter) for 88 days. These two diets were one with a high lipid level (∼17%, HL) and a moderate starch content (∼14%, LC), and the other being devoid of fish oil (4% lipid, LL) and with high starch content (∼23%, HC). Surprisingly, feeding Senegalese sole the HL/LC diet resulted in prolonged hyperglycaemia, while fish fed on LL/HC diet restored basal glycaemia 2 h after feeding. The hyperglycaemic phenotype was associated with greater glucose-6-phosphatase activity (a key enzyme of hepatic glucose production) and lower citrate synthase activity in the liver, with significantly higher liver glycogen content. Sole fed on HL/LC diet also had significantly lower hexokinase activity in muscle, although hexokinase activity was low with both dietary treatments. The HL/LC diet was associated with significant reductions in muscle AKT, p70 ribosomal S6-K1 Kinase (S6K-1) and ribosomal protein S6 (S6) 2 h after feeding, suggesting down regulation of the AKT-mTOR nutrient signalling pathway in these fish. The results of this study show for the first time that high level of dietary lipids strongly affects glucose metabolism in Senegalese sole.
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