An intensive isolation program carried out in three replicated composting piles allowed the identification of the resident and transient components of the composting microbiome. More than 4000 ...bacterial strains were isolated, enzymatically characterized and identified by partial sequencing of their 16S rRNA gene. While microorganisms isolated under mesophilic conditions were prominent throughout the process, thermophilic stages gathered the highest total counts and spore-forming bacteria prevailed at the bio-oxidative phase of composting. Enzymatic capabilities related to the degradation of polymeric materials were exhibited by most of the isolates and as a result of these activities, more soluble compounds could be made available to the entire composting microbiota. A high proportion of isolates showed to be thermotolerant as they were detected at mesophilic and thermophilic phases. Isolated strains belonged to 187 bacterial species. Biodiversity was greater at the central stages of composting and mesophilic, thermophilic and cooling phases shared 50% of species.
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•Intense turnover of organic matter was induced by inoculation of composting piles.•More simple compounds were released from polymeric fractions in inoculated piles.•Humification was ...more intense and earlier achieved in inoculated piles.•Inoculation clearly stimulated growth and activity of the composting microbiota.•Inoculation affected microbiota structure but its functionality remained unaltered.
Enhanced organic matter turnover was detected in lignocellulosic composting piles inoculated with microorganisms specifically capable of decomposing polymeric compounds. In comparison to uninoculated piles, the following results were obtained in the inoculated piles: degradation of hemicellulose, cellulose and lignin were 28%, 21% and 25% respectively higher. Total organic matter, total sugars and phenolic compounds also decreased more intensely. Greater amounts of soluble organic carbon, reducing sugars and soluble proteins were available to the composting microbiota. Recycling of organic to inorganic nitrogen was improved and humification was more intense and earlier attained. Microbial community structure was also affected by inoculation. It was initially thought that these effects were due to enzymatic capabilities of inoculants, however, microbial counts, especially those corresponding to functional groups, revealed that inoculation induced a true stimulation of microbial growth and activity in the entire composting microbiota which was actually responsible for all the beneficial effects reported here.
Many alternatives for the proper disposal of horticultural plant wastes have been studied, and composting is one of the most attractive due to its insignificant environmental impact and low cost. The ...quality of compost for agronomical use is related to the degree of organic matter maturation and stabilization. Traditional parameters as well as temperature, ratio C/N, cationic exchange capacity, extractable carbon, or evolution of humificated substances have been successfully used to assess compost maturity and stability. However, microorganisms frequently isolated during composting release a wide range of hydrolytic enzymes, whose activity could apparently give interesting information on the rate of decomposition of organic matter and, therefore, on the product stability. The aim of this work was to study the evolution of some important enzymatic activities during composting of agricultural wastes and their comparison with other chemical parameters commonly employed as quality and maturity indexes, to establish a relationship between the degradation intensity of specific organic carbon fractions throughout the process. In this work, the chemical and biochemical parameters of plant wastes were studied along a composting process of 189 days to evaluate their importance as tools for compost characterization. Results showed an intense enzymatic activity during the first 2–3 weeks of composting (bio-oxidative phase), because of the availability of easily decomposable organic compounds. From a biological point of view, a less intense phase was observed between second and third month of composting (mesophilic or cooling phase). Finally, chemical humification parameters were more closely associated with the period between 119 and 189 days (maturation phase). Significant correlations between the enzymatic activities as well as between enzyme activities and other more traditional parameters were also highlighted, indicating that both kind of indexes can be a reliable tool to determine the degree of stability and maturation of horticultural plant wastes based-compost.
•Enzymatic and temperature patterns registered inside the pile are directly related.•Enzymatic activity reaches its peak during the first days of the composting process.•When piles begin to cool, the enzymatic activity decreases noticeably.•The intensity of the enzymatic activity indirectly affects the humification process.
A biological comparison based on differences in the starting material for composting processes was made. Mesophilic aerobic microbiota, fungi, actinomycetes and hemicellulolytic microorganisms ...reached significantly higher levels in the MSW final product. The population of cellulolytic microorganisms did not show a clear trend, although it was more numerous in the HW piles. Counts for N
2-fixing and ammonifying bacteria were significantly higher in the SS pile at the early stages of the process, while populations tended to become equal as time progressed. The lowest populations were detected for nitrifying bacteria, with higher but not always significant levels for the SS pile. β-Glucosidase and phosphatase activities were higher in the SS pile at the early stages. Protease reached its maximum activity during the bio-oxidative phase and final stages in the HW and MSW piles, respectively. Dehydrogenase activity, with an occasional high level for the MSW at the beginning of the process, was almost inexistent since the end of the bio-oxidative phase. On the contrary, urease showed higher levels at the final stage of the process, with the MSW pile showing the greatest levels most of the time. According to these results, the nature of the starting material causes differences in biological parameters.
Serious game-based learning coupled with just-in-time teaching strategies was applied by offering six different questionnaires to evaluate student knowledge about the safety issues and theoretical ...concepts and protocols of laboratory exercises. The questionnaires were presented with an appearance similar to that of computer games by means of the Kahoot! platform. Passing the safety questionnaire was mandatory, and high scores were achieved by all the students, ensuring they all properly knew the laboratory safety rules. We consider this point essential in chemistry and engineering education, because future professionals must be aware of the importance of safety. In addition, responses to the questionnaires regarding the theory and procedures of each laboratory session allowed us to detect the main problems students had in the acquisition of the theoretical concepts before starting the practical activities. Additionally, the satisfaction of teachers and students with this methodology was also evaluated through surveys in the Moodle platform, showing consensus about the fact that the questionnaires are a valuable tool.
A study was carried out to identify biogeochemical reactions along a transect of a coastal dolomitic aquifer. In this transect, the physicochemical parameters of the groundwater as well as the ...microbial composition of samples taken at different depths and salinities were measured. Many of the dissolved ions measured in the groundwater follow a pattern that reflects the distribution of the water masses (fresh, interface and salt) in the aquifer, while others such as Ca and Mg ions deviate from this trend by identifying the zones of maximum dissolution of the carbonate matrix. The concentrations of minor ions, such as Fe and Mn, also follow a singular pattern, with maximum concentrations in the reducing zones of the aquifer and lower values in the oxidizing zones. Precipitates of Mn oxides along with other metals, such as Fe, Ba, Zn and Ni, were observed in the saline zone displaying oxidizing conditions close to the coastline, where a continuous core was recovered. This zone, which is located below the freshwater-seawater mixing zone and features percentages of seawater higher than 80 %, is characterized by the presence of Marinobacter as the predominant genus. These bacteria are also related to the formation of Mn-rich polymetallic oxides in other contexts such as the ocean floor (Wang et al., 2012; Cao et al., 2021). All in all, a biogeochemical reaction model is proposed that describes the formation of these oxides in areas close to the discharge zone of coastal aquifers. To do this, it has been necessary to integrate the results obtained from geochemical, hydrogeological and microbiological information.
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•The geochemistry and microbiota of a dolomitic coastal aquifer has been studied.•Groundwater samples have been taken from several boreholes along the path flow.•MnO2 precipitation happens in saline and oxidative areas next to the coastal line.•Marinobacter and other genera can be the responsible of the MnO2 precipitation.
•Screening of drugs in urine samples with direct injection is carried out.•Retention models are developed for micellar eluents with extended solvent domains.•Isocratic, linear and multi-linear ...separations are interpretively optimised.•Consequences of surfactant desorption in C8 and C18 columns by a gradient are shown.•Excellent agreement between predictions and experimental chromatograms is found.
Micellar liquid chromatography (MLC) is a reversed-phase mode with mobile phases containing an organic solvent and a micellised surfactant. Most procedures developed in MLC are implemented in the isocratic mode, since the general elution problem in chromatography is less troublesome. However, gradient elution may be still useful in MLC to analyse mixtures of compounds within a wide range of polarities, in shorter times. MLC using gradients is attractive to determine by direct injection moderate to low polar compounds in physiological samples. In these analyses, the use of initial micellar conditions (isocratic or gradient) with a fixed amount of surfactant above the critical micellar concentration, keeping the organic solvent content low, will provide better protection of the column against the precipitation of the proteins in the physiological fluid. Once the proteins are swept away, the elution strength can be increased using a positive gradient of organic solvent to reduce the analysis time. This may give rise to the transition from the micellar to the submicellar mode, since micelles are destroyed at sufficiently high concentration of organic solvent. In this work, several retention models covering extended solvent domains in MLC are developed and tested, and applied to investigate the performance in isocratic, linear and multi-linear gradient separations. The study was applied to the screening of β-adrenoceptor antagonists in urine samples, using mobile phases prepared with sodium dodecyl sulphate and 1-propanol. Predicted chromatograms were highly accurate in all situations, although suffered of baseline problems and minor shifts for peaks eluting close to a steep gradient segment. Two columns (C18 and C8) were investigated, with the C8 column being preferable owing to the smaller amount of adsorbed surfactant.
A study to determine total and mobile heavy metals concentrations in sediments from Algeciras Bay was performed and pollution hotspots were identified. The effects on aquatic organisms were ...established using sediment quality guidelines (SQGs). Ni and Cr exceeded the effect range medium and low levels, respectively, around industrial area. Potential toxicity of metals was determined by diethylenetriaminepenta-acetic acid (DTPA) extractions and low alert levels of Co, Cu, Zn, Cd, Ni and Pb were exceeded at most sampling sites. Three pollution indicators were used showing significant values for As, Ni, Cr, Pb and Cd. Sediment speciation using the sequential extraction BCR procedure was carried out, being Cd, Zn, Pb and As the most available metals. Principal component, cluster and ANOVA analyses were performed in order to assess the sources of metals and the influence of seasonality and anthropogenic activities on the sediment quality. Two principal component analysis (PCA) factors were obtained identifying the sampling sites affected by anthropogenic activities; Ni–Cr and Zn–Cu–V clusters were also obtained associated with stainless steel and petrochemical industrial activities. ANOVA showed the outstanding sites because of total metal concentration and significant differences among sampling sites by the acid extractable and reducible fractions for all metals except for Ba and V.
The aim of this study was to monitor some microbial indicators and pathogen contents (sulphite reducers clostridia, total enterobacteriaceae, total coliforms, faecal coliforms (Escherichia coli), ...enterococci, Staphylococcus aureus and Salmonella spp.) throughout the co-composting of wastes from the winery and distillery industry with other organic residues, as well as the effect of the composting system used. Seven different piles using mixtures of winery–distillery wastes with other organic materials were prepared. P1 and P2 were made using grape stalk (GS), grape marc (GM), exhausted grape marc (EGM) and sewage sludge (SS), whereas in P3 and P4 were also used exhausted grape marc with cow manure (CW) and poultry manure (PM), respectively, using the Rutgers system. Additionally, P2 was watered with vinasse (V). The rest of piles (P5, P6 and P7) were prepared with grape marc, exhausted grape marc, cow manure and poultry manure, using the turning system. The effectiveness of the composting process to reduce the pathogen content was higher in the static aerated piles than in those elaborated with the turning. The relatively high temperatures (50–60°C) reached in some of the piles produced a notable decrease in some microbial groups, such as total and faecal coliforms (E. coli), but the characteristics of the raw materials used notably influenced the pathogen contents of the end-product.
We present the first catalog of TeV gamma-ray sources realized with data from the newly completed High Altitude Water Cherenkov Observatory (HAWC). It is the most sensitive wide field-of-view TeV ...telescope currently in operation, with a one-year survey sensitivity of ∼5%-10% of the flux of the Crab Nebula. With an instantaneous field of view >1.5 sr and >90% duty cycle, it continuously surveys and monitors the sky for gamma-ray energies between hundreds of GeV and tens of TeV. HAWC is located in Mexico, at a latitude of 19° N, and was completed in 2015 March. Here, we present the 2HWC catalog, which is the result of the first source search performed with the complete HAWC detector. Realized with 507 days of data, it represents the most sensitive TeV survey to date for such a large fraction of the sky. A total of 39 sources were detected, with an expected number of false detections of 0.5 due to background fluctuation. Out of these sources, 19 are new sources that are not associated with previously known TeV sources (association criteria: <0 5 away). The source list, including the position measurement, spectrum measurement, and uncertainties, is reported, then each source is briefly discussed. Of the 2HWC associated sources, 10 are reported in TeVCat as PWN or SNR: 2 as blazars and the remaining eight as unidentified.
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