Potential and constraints of different seawater and freshwater blends as growing media for three vegetable crops Atzori, Giulia; Werther Guidi NissimauthorDepartment of Agrifood Production and Environmental Sciences (DISPAA) University of Florence, Viale delle Idee 30, Sesto Fiorentino, Florence 50019, Italy; Stefania CaparrottaauthorDepartment of Agrifood Production and Environmental Sciences (DISPAA) University of Florence, Viale delle Idee 30, Sesto Fiorentino, Florence 50019, Italy ...
2015
Journal Article
The perception of food quality. Profiling Italian consumers Mascarello, Giulia; Anna PintoauthorIstituto Zooprofilattico Sperimentale delle Venezie, Health Awareness and Communication Department, Viale dell'Università 10, 35020 Legnaro (PD), Italy; Nicoletta PariseauthorUniversity of Padua, Faculty of Political Science, Via Cesarotti 10/12, 35123 Padova, Italy ...
2015
Journal Article
These experiments were designed to define the ability of human TCR-gamma+ cells to recognize allogeneic cells. TCR-gamma+-enriched populations were obtained by treating peripheral blood E-rosetting ...cells with anti-CD4 and anti-CD8 mAbs. The resulting populations were CD2+4-8- expressed variable proportions of CD3+ cells (40-90%), and did not react with the WT31 mAb, which is specific for a framework determinant of the alpha/beta heterodimer that serves as receptor for antigen on most human T lymphocytes. After mixed lymphocyte culture with irradiated allogeneic cells for 7 d and 3 additional days in rIL-2 (100 U/ml), cells underwent proliferation in three of five individuals tested. In addition, MLC-derived cells lysed 51Cr-labeled PHA-induced blasts derived from the allogeneic cells used as stimulator, but not allogeneic unrelated or autologous blast cells. No cytotoxicity against autologous or allogeneic target cells could be induced by culturing CD3+4-8-WT31- lymphocytes in MLC with irradiated autologous cells. Surface iodination of allogeneic MLC-activated CD3+4-8-WT31- cells followed by lysis in 1% digitonin and immunoprecipitation with anti-CD3 mAb indicated that the CD3-associated molecules consisted of a major 45-kD band and a minor band of 43 kD. Northern blot analysis showed that mRNA for the gamma chain was expressed at high levels, whereas mRNAs for alpha and beta chains were missing. These data support the notion that TCR-gamma rather than TCR-alpha/beta is expressed in allospecific CD3-4-8-WT31- cell populations. Clones were further derived from MLC-stimulated CD3+4-8-WT31- populations. All the seven clones studied in detail maintained the surface phenotype as well as the cytolytic pattern of the original MLC populations, thus only specific allogeneic PHA-induced blasts were lysed. NK-sensitive as well as NK-resistant tumor targets were variably susceptible to lysis; therefore, specific cytolytic activity against allogeneic cells was not necessarily linked to the expression of MHC-nonrestricted cytotoxicity against tumor cells.
Profiling chicory sesquiterpene lactones by high resolution mass spectrometry Graziani, Giulia; Rosalia FerracaneauthorDepartment of Agricultural and Food Science, University of Napoli Federico II, Parco Gussone, 80055 Portici, Napoli, Italy; Paolo SamboauthorDepartment of Agronomy, Food, Natural resources, Animals and Environment (DAFNAE), Agripolis University of Padova, Viale dell'Universit 16, 35020 Legnaro, PD, Italy ...
2015
Journal Article
BB3 and δ -TCS1 monoclonal antibodies identify two distinct nonoverlapping populations of T-cell receptor (TcR) γ /δ (TcR-1)-positive cells, which express a disulfidelinked and a nondisulfide-linked ...form of TcR, respectively. BB3+ cells represented the majority of circulating TcR-1+ cells, but they were virtually undetectable in the thymus. On the other hand, δ -TCS1+ cells were largely predominant among TcR-1+ thymocytes but represented a minority in peripheral blood (PB). Similar distributions were observed by clonal analysis of thymocytes or PB TcR-1+ populations. The use of joining region (J)-specific probes indicated that BB3+ and δ -TCS1+ clones displayed different patterns of J rearrangement. Thus, the disulfide-linked form of TcR-1 (BB3+ clones) was associated with the expression of J segments upstream to the Cγ1 gene segment, whereas the nondisulfide-linked form (δ -TCS1+ clones) was associated with the expression of J segments upstream to Cγ2. δ -TCS1+ clones, in most instances, exhibited a growth pattern different from that of BB3+ or conventional TcR α /β + clones as they adhered promptly to surfaces, spread, and emitted long filopodia ending with adhesion plaques. Ultrastructural analyses showed, exclusively in δ -TCS1+ cells, nuclear deformations, uropod formation, and abundant cytoskeletal structures. In addition, immunofluorescence studies of this subset of TcR-1+ cells revealed the presence of abundant microtubules, intermediate filaments, and submembranous microfilaments. Thus, our findings suggest that δ -TCS1+ cells are capable of active motility.
The aim of the study was to compare the short and long-term outcomes of older and younger colorectal cancer patients with advanced disease resected with a curative intent. Six hundred and ninety-two ...patients were analysed. Four hundred and seventy-nine patients were younger than 70 years (Group 1), and 213 were 70 years of age or above (Group 2). The overall perioperative mortality rate in the younger group was 0.8% (n = 7), as against 1.4% (n = 3) in the elderly group (p = NS). The morbidity rates were 35% and 42%, respectively (p = NS). At univariate analysis, the elderly patients had a worse overall survival compared to the younger group, when only patients undergoing postoperative chemo-radiotherapy were considered (54% vs 67% overall survival at 5 years; p = 0.03). Using logistic regression analysis, tumour stage (p < 0.0001) and radicality of surgery (p < 0.0001) correlated significantly with overall survival rates in the elderly. Colorectal surgery for malignancy can be performed safely in the elderly with acceptable morbidity and mortality rates and long-term survival.
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