The Bohai Bay Basin entered a late stage of structural depression during the sedimentary depositional period of the lower member of the Minghuazhen Formation in the Bozhong area of the Huanghekou ...Sag, China. Numerous analyses of geological and geophysical data have shown that the climate was relatively humid in the Bozhong area. Additionally, the basin basement subsided at a stable rate, creating a broad basin with gentle slopes. The research area in this study was close to the sediment source, which supplied it with a sufficient amount of sediment. The lake shoreline changed relatively frequently, and the lake was deep, which allowed shallow-water deltas to develop widely. The reticular shallow-water delta in the research area has the following sedimentary characteristics: (1) the fine-grained lithology, low component and textural maturities, and the cumulative probability particle size curve suggest tractive flow deposition. (2) Deltas with low sandstone contents and widely distributed red mudstone are widespread. (3)
Chaetosphaeridium
and biological disturbances are common. (4) The lake level fluctuates frequently. (5) Delta-front subfacies are mainly developed in the Bozhong area; they show a reticular planar distribution, comprise composite sandbodies, and are superposed by multi-stage underwater distributary channels. (6) The river channel is narrow and developed a large number of main river channels in different directions. (7) The underwater distributary channel microfacies are distributed within multibranched and bifurcated channels and generally show a discontinuous positive rhythm; however, the estuary dam sandbody is not well developed. This work suggests that a reticular shallow-water delta sedimentary system formed under humid climate conditions in the Bozhong area and is characterized by its near-source type, fine granularity, wide distribution, and vertically and horizontally overlapping of underwater distributary channel sandbodies. During the exploration process, this sedimentary system can provide a reasonable guide with which to predict the sandbody distribution in a lithologic reservoir.
Metal homeostasis is critical for plant growth, development and adaptation to environmental stresses and largely governed by a variety of metal transporters. The plant ZIP (Zn-regulated transporter, ...Iron-regulated transporter-like Protein) family proteins belong to the integral membrane transporters responsible for uptake and allocation of essential and non-essential metals. However, whether the ZIP family members mediate metal efflux and its regulatory mechanism remains unknown.
In this report, we provided evidence that OsZIP1 is a metal-detoxified transporter through preventing excess Zn, Cu and Cd accumulation in rice. OsZIP1 is abundantly expressed in roots throughout the life span and sufficiently induced by excess Zn, Cu and Cd but not by Mn and Fe at transcriptional and translational levels. Expression of OsZIP-GFP fusion in rice protoplasts and tobacco leaves shows that OsZIP1 resides in the endoplasmic reticulum (ER) and plasma membrane (PM). The yeast (Saccharomyces cerevisiae) complementation test shows that expression of OsZIP1 reduced Zn accumulation. Transgenic rice overexpressing OsZIP1 grew better under excess metal stress but accumulated less of the metals in plants. In contrast, both oszip1 mutant and RNA interference (RNAi) lines accumulated more metal in roots and contributed to metal sensitive phenotypes. These results suggest OsZIP1 is able to function as a metal exporter in rice when Zn, Cu and Cd are excess in environment. We further identified the DNA methylation of histone H3K9me2 of OsZIP1 and found that OsZIP1 locus, whose transcribed regions imbed a 242 bp sequence, is demethylated, suggesting that epigenetic modification is likely associated with OsZIP1 function under Cd stress.
OsZIP1 is a transporter that is required for detoxification of excess Zn, Cu and Cd in rice.
The identification of biomarkers and effective therapeutic targets for gastric cancer (GC), the most common cause of cancer-related deaths around the world, is currently a major focus in research. ...Here, we examined the utility of LHFPL6 as a prognostic biomarker and therapeutic target for GC.
We explored the clinical relevance, function, and molecular role of LHFPL6 in GC using the MethSurv, cBioPortal, TIMER, Gene Expression Profiling Interactive Analysis, ONCOMINE, MEXPRESS, and EWAS Atlas databases. The GSE118919, GSE29272, and GSE13861 datasets were used for differential expression analysis. Using The Cancer Genome Atlas, we developed a Cox regression model and assessed the clinical significance of LHFPLs. In addition, we used the "CIBERSORT" algorithm to make reliable immune infiltration estimations. Western blot and immunohistochemistry were used to examine protein expression. Cell migration and invasion were assessed using transwell experiments. THP-1-derived macrophages and GC cells were co-cultured in order to model tumor-macrophage interactions in vitro. The levels of CD206 and CD163 were measured using immunofluorescence assays. The results were visualized with the "ggplot2" and "circlize" packages.
Our results showed that in GC, LHFPL6 overexpression was significantly associated with a poor prognosis. Our findings also suggested that LHFPL6 may be involved in the activation of the epithelial-mesenchymal transition. Furthermore, LHFPL6 expression showed a positive correlation with the abundance of M2 macrophages, which are potent immunosuppressors.
LHFPL6 could be a prognostic biomarker and therapeutic target for GC.
Regulating the luminescence color of single fluorescent component via non-covalent interactions has attracted considerable attentions in recent years, as its dynamic and controllable nature is ...favorable to develop smart luminescent materials. Herein, a supramolecular photoluminescence system with tunable fluorescence emissions ranging from yellow to green was successfully fabricated by assembling a simple fluorescent molecule, 4-(pyren-1-yl)-1-(4-vinylbenzyl)pyridin-1-ium chloride (PPD), and cucurbitnuril (CBn, n = 7, 8) via host-guest interactions. Upon this assembly, PPD possessed a dramatically enhanced emission efficiency as well as a longer fluorescence lifetime. Such dynamically controllable properties with tunable fluorescence emissions enabled PPD-based assembly to be applied as fluorescent ink and have a potential in information encryption material.
Display omitted
•A supramolecular photoluminescence system with tunable emissions was fabricated.•The fluorophore could bind with cucurbit 7uril and cucurbit 8uril differently.•The quantum yield of luminophore enhanced dramatically after complexing with hosts.•A new kind of security ink was designed using dynamic nature of host-guest complex.
Abstract
A nanoscale vector magnetometer is demonstrated by integrating a microfabricated diamond probe with a nanopillar on top of a single‐mode optical fiber. Based on the recently developed ...self‐aligned patterning techniques, ≈80 nitrogen vacancies are concentrated at a 100 nm
2
area at the center of nanopillars to maximize the excitation and collection efficiencies. The spatial resolution of such a fiber‐coupled diamond magnetometer can reach 100 nm for the first time with a sensitivity of 11.27 µT/ for DC magnetic field sensing. In addition, the probe can provide deterministic oriented diamond nanopillars for simplified vector magnetometry. This fiber‐coupled diamond probe is applied to demonstrate the magnetic field imaging of charged electrodes in a confined box. These results mark a decisive step toward fiber‐based applications with nanoscale vector magnetometry capabilities.
Although previous studies have considered rumination a possible mediator of the relationship between mental health and problematic smartphone use (PSU), few prospective studies have been conducted, ...limiting the ability to draw causal inferences. Therefore, the current study aimed to examine the mediating role of rumination on the depression-PSU relationship using three-wave cross-lagged panel models (CLPMs) with longitudinal data.
A sample of 321 medical students from China Medical University completed three waves of online measures of depressive symptoms, rumination, and PSU. The three-wave CLPMs were constructed to examine the mediating role of rumination.
Our results demonstrated that depressive symptoms were bidirectionally related to rumination, and that rumination was bidirectionally related to PSU. The reciprocal CLPM suggested that depressive symptoms at Time 1 positively affected PSU at Time 3 via rumination at Time 2. The indirect effect was significant, with a path coefficient of 0.023 (95% CI: 0.004 to 0.042). Conversely, PSU at Time 1 positively affected depressive symptoms at Time 3 via rumination at Time 2, with a path coefficient of 0.015 (95% CI: 0.001 to 0.029).
This prospective study provided empirical evidence of the influence of depression on PSU and vice versa among Chinese university students. It also highlighted the importance of rumination in the depression-PSU relationship, revealing a bidirectional mediating role of rumination. Additional large-scale multi-wave longitudinal studies are needed to verify our results.
Breast cancer (BC) has become one of the most common malignant tumors in the world, seriously endangering women's health and life. However, the underlying molecular mechanisms of BC remain unclear. ...Over the past decade, long non-coding RNAs (lncRNAs) were gradually discovered and appreciated to play pivotal regulatory role in the progression of BC. It has been demonstrated that lncRNAs are implicated in regulating plenty of biological phenomena including cell proliferation, apoptosis, invasion and metastasis by interacting with DNA, RNA or proteins. In addition to these, the function of lncRNAs in tumor resistance has increasingly attracted more attention. In this review, we summarized the emerging impact of lncRNAs on the occurrence and progression of human BC, specifically focusing on the functions and mechanisms of them, with the aim of exploring the potential value of lncRNAs as oncogenic drivers or tumor suppressors. Furthermore, the potential clinical application of lncRNAs as diagnostic biomarkers and therapeutic targets in BC was also discussed.
HPP (heavy metal associated plant protein) and HIPP (heavy metal associated isoprenylated plant protein) are a group of metal-binding metallochaperones playing crucial roles in metal homeostasis and ...detoxification. Up to now, only few of them have been functionally identified in plants. Here, we identified 54 HPP and HIPP genes in rice genome. Analysis of the transcriptome datasets of the rice genome exposed to cadmium (Cd) revealed 17 HPP/HIPP genes differentially expressed, with 11 being upregulated (>2 fold change, p < 0.05). Comprehensive analysis of transcripts by qRT-PCR showed that both types of genes displayed diverse expression pattern in rice under excess manganese (Mn), copper (Cu) and Cd stress. Multiple genomic analyses of HPPs/HIPPs including phylogenesis, conserved domains and motifs, genomic arrangement and genomic and tandem duplication were performed. To identify the role of the genes, OsHIPP16, OsHIPP34 and OsHIPP60 were randomly selected to express in yeast (Saccharomyces cerevisiae) mutants pmrl, cup2, ycf1 and zrc1, exhibiting sensitivity to Mn, Cu, Cd and Zn toxicity, respectively. Complementation test showed that the transformed cells accumulated more metals in the cells, but their growth status was improved. To confirm the functional role, two mutant oshipp42 lines defective in OsHIPP42 expression were identified under metal stress. Under normal condition, no difference of growth between the oshipp42 mutant and wild-type plants was observed. Upon excess Cu, Zn, Cd and Mn, the oshipp42 lines grew weaker than the wild-type. Our work provided a novel source of heavy metal-binding genes in rice that can be potentially used to develop engineered plants for phytoremediation in heavy metal-contaminated soils.
Display omitted
•We identified 54 HPP/HIPP genes in rice genome with 11 upregulated under Cd stress.•Diverse HPP/HIPP expression pattern in rice under excess metal stress was observed.•Expression of HIPPs in yeast mutants grew better and accumulated more metals than WT.•The oshipp42 lines showed a weaker growth than WT under metal stress.
•An efficient seq-HS-SBSE approach to extract volatiles in green teas was developed.•38 important volatile enantiomers in baked green teas were evaluated by Es-GC-O/MS.•R-Linalool enhanced “floral”, ...“sweet” and “chestnut-like” aromas of baked green tea.•R-Limonene mainly contributed to “sweet” and “clean” aromas of baked green tea.•S-α-Terpineol promoted the “sweet” and “floral” aromas of baked green tea.
Chiral volatile compounds are known to be distributed in teas at various enantiomeric ratios. However, the performance of each enantiomer, including aroma characteristics, aroma intensities, and contribution to the overall flavor of tea, is still unclear. In this study, aroma characteristics and intensities of 38 volatile enantiomers in standards and baked green teas with chestnut-like aroma and clean aroma were evaluated by an efficient sequential headspace-stir bar sorptive extraction (seq-HS-SBSE) approach combined with the enantioselective gas chromatography-olfactometry/mass spectrometry (Es-GC-O/MS) technique. Moreover, aroma recombination results for the two types of baked green teas using 14 chiral odorants and four achiral odorants indicated that the combinations of the detected odorants mainly contributed to the “floral”, “sweet”, and “chestnut-like” aromas. R-Linalool simultaneously enhanced the “floral”, “sweet”, and “chestnut-like” aromas; R-limonene mainly contributed to the “sweet” and “clean” aromas; and S-α-terpineol promoted the “sweet” and “floral” aromas of baked green tea.
PDF
