Polydopamine (PDA) nanoparticles have emerged as an attractive biomimetic photothermal agent in photothermal antibacterial therapy due to their ease of synthesis, good biodegradability, long‐term ...safety, and excellent photostability. However, the therapeutic effects of PDA nanoparticles are generally limited by the low photothermal conversion efficiency (PCE). Herein, PDA@Ag nanoparticles are synthesized via growing Ag on the surface of PDA nanoparticles and then encapsulated into a cationic guar gum (CG) hydrogel network. The optimized CG/PDA@Ag platform exhibits a high PCE (38.2%), which is more than two times higher than that of pure PDA (16.6%). More importantly, the formulated CG/PDA@Ag hydrogel with many active groups can capture and kill bacteria through effective interactions between hydrogel and bacteria, thereby benefiting the antibacterial effect. As anticipated, the designed CG/PDA@Ag system combined the advantages of PDA@Ag nanoparticles (high PCE) and hydrogel (preventing aggregation of PDA@Ag nanoparticles and possessing inherent antibacterial ability) is demonstrated to have superior antibacterial efficacy both in vitro and in vivo. This study develops a facile approach to boost the PCE of PDA for photothermal antibacterial therapy, providing a significant step forward in advancing the application of PDA nano‐photothermal agents.
This work presents a facile and convenient strategy to boost the photothermal conversion efficiency of polydopamine (PDA) nanomaterials via growing Ag nanoparticles on the surface of PDA and then encapsulated into a cationic guar gum hydrogel network. The resultant system possesses robust photothermal performance that effectively kills bacteria and promotes wound healing.
Polydopamine nanoparticles (PDA NPs) are an appealing biomimetic photothermal agent for photothermal antibacterial treatment because of their long-term safety, excellent photostability, accessible ...manufacturing, and good biodegradability. However, the low photothermal conversion efficiency (PCE) of PDA NPs requires high-power and long-term near-infrared light irradiation, which severely restricts their practical application. In this work, PDA@Cu NPs were fabricated by growing Cu NPs in situ on the surface of PDA and then introduced into a polyelectrolyte hydrogel precursor (cationic polyethyleneimine/anionic pectin, named as CPAP). The formulated photothermal platform possessed a high PCE (55.4%), almost twice as much as pure PDA NPs (30.8%). Moreover, the designed CPAP/PDA@Cu captured and killed some bacteria by electrostatic adsorption, which helped enhance the antibacterial performance. As expected, the formed CPAP/PDA@Cu that combined the advantageous features of PDA@Cu NPs (high PCE) and CPAP matrix (inherent antibacterial activity and preventing NPs aggregation) can efficiently kill bacteria both in vitro and in vivo under the help of near-infrared laser irradiation. Taken together, this study offers a promising strategy for constructing a facile and safe PDA-based photothermal agent for photothermal antibacterial therapy.
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•A facile polyelectrolyte photothermal antibacterial platform (CPAP) was synthesized.•CPAP is composed of polyethyleneimine, pectin and polydopamine@Cu nanoparticles.•CPAP displayed good biocompatibility and tunable physicochemical properties.•CPAP possessed outstanding high-efficiency bacteria-killing capability.
The humpback grouper (
Cromileptes altivelis
) is a commercially important species of the family Epinephelidae. With the development in aquaculture industry,
C. altivelis
breeding has gradually ...increased in volumetric production, leading to the occurrence of various diseases. In this study, we established a new cell line (CAM) derived from the muscle tissue of
C. altivelis
. Our results showed that the optimal growth temperature and working concentration of fetal bovine serum (FBS) of CAM cells were 28 °C and 15%, respectively. DNA sequencing and comparative analysis of 18S rRNA gene sequence showed that CAM cell line was originated from
C. altivelis.
Chromosome analysis showed that the modal chromosome number of CAM cells was 48. After transfection using pEGFP-N3 plasmid, CAM cells exhibited high transfection efficiency, indicating that CAM cells could be used in foreign gene expression studies. Further, cytotoxicity analysis revealed that CAM cells were sensitive to
Vibrio harveyi
and
Edwardsiella tarda
. Moreover, the cytotoxicity of heavy metals (Hg, Cd, and Cu) to CAM cells was dose-dependent. This CAM cell line might be used as an ideal tool in vitro for analyzing and understanding the mechanisms of pathogenesis, host-pathogen interactions, and toxicity assay of heavy metals.
Current diabetic wound treatments remain difficult in clinical due to bacterial infection and excessive reactive oxygen species (ROS). An ideal wound dressing should have prominent antibacterial and ...ROS scavenging abilities. In this work, a conveniently multifunctional hydrogel wound dressing (abbreviated as CuTA/SG) was constructed by incorporating copper/tannic acid nanosheets into a dynamic cross-linking network composed of oxidized sodium alginate, gelatin, and Ca2+. The resulting CuTA/SG hydrogel had excellent exudate absorption, adjustable photothermal antibacterial, and ROS scavenging features. Furthermore, CuTA/SG hydrogel was injectable that possessed excellent adaptability to adapt to the wound defects due to its appropriate physical cross-linking. Astonishingly, CuTA/SG hydrogel showed good hemostasis, antibacterial, anti-inflammation, and even hair regeneration capabilities, which can significantly promote diabetic wound healing in the full-thickness skin defect model in rats through reducing inflammation, guiding wound repair, and restoring skin physiological functions. Therefore, this study develops a simple and effective strategy for fabricating highly effective hydrogel dressing, which might provide new options for clinical wound management.
Oral ulcers can be managed using a variety of biomaterials that deliver drugs or cytokines. However, many patients experience minimal benefits from certain medical treatments because of poor ...compliance, short retention times in the oral cavity, and inadequate drug efficacy. Herein, we present a novel hydrogel patch (SCE2) composed of a biopolymer matrix (featuring ultraviolet-triggered adhesion properties) loaded with cuttlefish ink nanoparticles (possessing pro-healing functions). Applying a straightforward local method initiates the formation of a hydrogel barrier that adheres to mucosal injuries under the influence of ultraviolet light. SCE2 then demonstrates exceptional capabilities for near-infrared photothermal sterilization and neutralization of reactive oxygen species. These properties contribute to the elimination of bacteria and the management of the oxidation process, thus accelerating the healing phase's progression from inflammation to proliferation. In studies involving diabetic rats with oral ulcers, the SCE2 adhesive patch significantly quickens recovery by altering the inflamed state of the injured area, facilitating rapid re-epithelialization, and fostering angiogenesis. In conclusion, this light-sensitive hydrogel patch offers a promising path to expedited wound healing, potentially transforming treatment strategies for clinical oral ulcers.
We present a hydrogel patch (SCE2) endowed with strong adhesive properties and immunomodulatory functions, designed for the treatment of oral ulcers in diabetes. Display omitted
•A hydrogel patch (SCE2) is engineered for treating oral ulcers in individuals with diabetes.•SCE2 is produced through the incorporation of cuttlefish ink nanoparticles within a biopolymer matrix.•SCE2 demonstrates potent capabilities in photothermal antibacterial and ROS scavenging.•SCE2 aids in the recovery of oral ulcers by diminishing inflammation and enhancing angiogenesis.
The wound healing process of the diabetic wound is often hindered by excessive oxygen free radicals and infection. An ideal wound dressing should possess great reactive oxygen species (ROS) ...scavenging property and considerable antibacterial ability. In this study, we facilely constructed a novel hydrogel dressing with excellent ROS scavenging property and outstanding antibacterial performance by introducing tannic acid (TA) into quaternized chitosan (QCS) matrix. Attributing to the suitable physical crosslinking between TA and QCS, this QCS/TA hydrogel was endowed with injectable and self-healing properties, which could avoid the various external squeezing on the irregular shape by wound dressing. The results showed that it could promote coagulation, suppress inflammation and expedite collagen deposition in the skin defect model of diabetic rats. This study provides a facile and convenient method for constructing injectable hydrogel dressing, which has application potentials in the clinical management of diabetic wounds.
•An injectable hydrogel (QT) composed of quaternized chitosan and tannic acid was designed.•One-step fabrication of QT hydrogel was achieved via alkali neutralization strategy.•QT hydrogel possessed robust antibacterial and ROS scavenging capabilities.•QT hydrogel significantly accelerated skin tissue regeneration in diabetic wounds.
•A robust hydrogel (GCM) was developed for treating bacteria-infected diabetic wounds.•GCM was synthesized by introducing melanin nanoparticles into a polysaccharide network.•GCM posed powerful ...photothermal antibacterial and ROS scavenging performance.•GCM facilitated wound healing by reducing inflammation and promoting angiogenesis.
Owing to hyperglycemia-related metabolic abnormalities that cause oxidative stress, recurrent infections, and vascular lesions, the therapeutic management of diabetic wounds is still unsatisfactory. Herein, we present a hydrogel (GCM) wound dressing loaded with melanin nanoparticles in a polysaccharide matrix (biguanide chitosan and oxidized β-glucan) for efficient healing of bacterially infected diabetic wounds. By a simple local injection, a protective GCM hydrogel barrier is established over the wound to rapidly stop bleeding and neutralize inflammatory cytokines. Subsequently, the GCM hydrogel achieves an accelerated transfer from the inflammatory process to the healing phase of the wound because of its excellent photothermal antimicrobial and reactive oxygen species scavenging properties. Additionally, GCM hydrogel has a three-dimensional network structure that dramatically enhances cell proliferation, migration, and angiogenesis by creating favorable ecological conditions for cell survival. Notably, our GCM hydrogel can help treat a variety of bacteria-infected diabetic wounds like skin and oral ulcer wounds. Collectively, this work provides a new option for diabetic wound treatment.
•A Summary of inorganic–organic nanomaterials for photothermal antibacterial therapy.•Common strategies for the construction of inorganic–organic nanomaterials.•Properties of inorganic–organic ...nanomaterials and photothermal antibacterial mechanisms.•Improvements in photothermal antibacterial therapy.
The clinical management of bacterial infections has become a growing global public health challenge. Such therapy is presently limited to antibiotics, but the misuse of antibiotics has led to multi-drug resistance and high mortality rates in anti-infective treatment. Therefore, it is critical to develop alternative biocides to open up new avenues of disinfection. Photothermal therapy (PTT), which usually kills bacteria by hyperthermia produced by photothermal agents (PTAs) under laser irradiation, has sparked increasing concern due to its low invasiveness, deep penetration, high selectivity, and avoidance of drug-resistant bacteria. Inorganic–organic hybrid nanomaterials that merge inorganic and organic elements can integrate the distinctive advantages of both while mitigating their innate shortcomings, displaying tremendous potential in the field of PTT antibacterial. In this review, we first briefly describe various strategies for fabricating functional inorganic–organic nanohybrid PTAs. Subsequently, the properties of the nanohybrid PTAs are discussed, covering characteristics from inorganic and organic parts, synergistic features, morphology dependence, and self-assembly of the nanohybrids. After that, the current status of nanohybrids for PTT antibacterial is demonstrated. Finally, we summarize and discuss some key issues, current challenges, and future developments in the rational synthesis of inorganic–organic nanohybrid PTAs for photothermal antibacterial therapy.
Current therapeutic protocols for diabetic foot ulcers (DFUs), a severe and rapidly growing chronic complication in diabetic patients, remain nonspecific. Hyperglycemia‐caused inflammation and ...excessive reactive oxygen species (ROS) are common obstacles encountered in DFU wound healing, often leading to impaired recovery. These two effects reinforce each other, forming an endless loop. However, adequate and inclusive methods are still lacking to target these two aspects and break the vicious cycle. This study proposes a novel approach for treating DFU wounds, utilizing an immunomodulatory hydrogel to achieve self‐cascade glucose depletion and ROS scavenging to regulate the diabetic microenvironment. Specifically, AuPt@melanin‐incorporated (GHM3) hydrogel dressing is developed to facilitate efficient hyperthermia‐enhanced local glucose depletion and ROS scavenging. Mechanistically, in vitro/vivo experiments and RNA sequencing analysis demonstrate that GHM3 disrupts the ROS‐inflammation cascade cycle and downregulates the ratio of M1/M2 macrophages, consequently improving the therapeutic outcomes for dorsal skin and DFU wounds in diabetic rats. In conclusion, this proposed approach offers a facile, safe, and highly efficient treatment modality for DFUs.
An immunomodulatory hydrogel with robust hyperthermia‐assisted self‐cascade glucose depletion and ROS scavenging properties is designed for treating diabetic foot ulcer wounds.
Polysaccharide hydrogel dressings are strong candidates for wound healing due to their low-cost, adsorbable, biodegradable, and biocompatible features. However, they may have some drawbacks, such as ...static properties, weak mechanics, and the inability to fully meet the requirements of the different phases of wound repair. Enhanced functionality achieved by incorporating nanomaterials into traditional polysaccharide networks is an effective way to address these challenges. Herein, an all-in-one hydrogel dressing (OQP2) that possessed hemostasis, antibacterial, reactive oxygen species scavenging, and angiogenic properties was readily synthesized by introducing poly(tannic acid) nanorods into a polysaccharide matrix comprising oxidized β-glucan and quaternary ammonium chitosan. As expected, the OQP2 can accelerate wound healing in diabetic rat models. This study offers a convenient and facile one-pot strategy to construct multifunctional polysaccharide hydrogel dressings for treating diabetic wounds.
•A hydrogel (OQP) dressing was fabricated for accelerating wound healing.•OQP composed of poly(tannic acid) nanorods and a polysaccharide matrix.•OQP possessed convenient, efficient, economical, injectable, and safe properties.•OQP can adsorb wound exudates, reduce inflammation, and guide tissue regeneration.
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