...improved viability is accompanied by an increased risk of unreversed injuries, such as bronchopulmonary dysplasia and intraventricular hemorrhage. ...a more physiological simulating in utero ...status is needed to protect EPIs' immature organs during the transitional period and ensure that they develop in the same manner that they would have in the womb. 2 Another term, artificial placenta (AP), was confused with AW in former studies. Since the term "womb" has a more abundant meaning than "placenta," we use AW instead of AP in this article. Importantly, a womb can perform multiple physiological functions, but gas exchange is the primary function of the current AW system. ...we also explored additional potential application scenarios of AW Figure 1 to minimize the preterm-related complications. ...it is worth noting that high-pressure ventilation in MV groups can increase intrathoracic pressure and impede venous drainage from the brain, leading to a higher possibility of venous hypertension and ICH,4 while in AW system using the jugular vein for cannulation appears to decrease cerebral blood flow. ...the use of heparin in the AW system may increase the risk of clinically significant cerebral hemorrhage, although no cases of ICH were observed during the experiment.
Co
3
O
4
-doped TiO
2
nanotube array electrodes were prepared by anodizing the Co–Ti alloys with different Co contents. Morphologies, elemental compositions, crystal structures, and electrochemical ...properties of the samples were characterized through scanning electron microscopy, X-ray photoelectron spectroscopy, X-ray diffraction, and electrochemical workstation. The effects of Co content, annealing temperature and testing electrolyte on the electrochemical properties of the electrodes were studied. Results show that the areal capacitance values of TiO
2
nanotube arrays were obviously improved by doping with Co
3
O
4
. The electrochemical properties of Co
3
O
4
-doped TiO
2
nanotube array electrodes were best when the Co content in the alloys was 9%; the annealing temperature was 100 °C and the testing electrolyte was 0.5 M Na
2
SO
4
. The Co
3
O
4
-doped TiO
2
nanotube array electrodes prepared under the optimal conditions had a high areal capacitance value of 937.9 µF cm
−2
when the scan rate was 10 mV s
−1
and the electrodes exhibited good rate and superior cycling performance.
Graphical abstract
MicroRNA-675-5p (miR-675-5p) is dysregulated in multiple human cancers, but its involvement in papillary thyroid cancer (PTC) remains to be investigated. This study aimed to examine the expression ...pattern of miR-675 in PTC, determine the effects of miR-675 on regulating the progression of PTC, and to explore the underlying molecular mechanisms.
The expression profile of miR-675 in PTC tissues and cell lines was determined using RT-qPCR. CCK-8, transwell migration and invasion assays, and xenograft tumors in nude mice were employed to analyze proliferation, in vitro migration and invasion, and in vivo tumor growth of PTC cells, respectively. The putative target of miR-675 was predicted using bioinformatic algorithms and was confirmed using luciferase reporter assays, RT-qPCR, and Western blotting.
miR-675 expression was decreased in PTC tissues and cell lines. A low level of miR-675 expression was significantly correlated with lymphatic metastasis and TNM stage in PTC patients. Ectopic miR-675 expression suppressed PTC cell proliferation, migration, and invasion in vitro and hindered tumor growth in vivo. Mitogen-activated protein kinase 1 (MAPK1) was found to be the direct target gene of miR-675 in PTC cells. MAPK1 reintroduction negated the tumor-suppressing effect of miR-675 overexpression in PTC cells. Furthermore, the lncRNA mitochondrial RNA processing endoribonuclease (RMRP) functioned as a ceRNA of miR-675 in PTC cells. Silencing RMRP expression inhibited the growth and metastasis of PTC cells by sponging miR-675 and regulating MAPK1.
These findings revealed that miR-675 directly targets MAPK1 and is sponged by lncRNA RMRP to inhibit the oncogenicity of PTC, suggesting the RMRP-miR-675-MAPK1 pathway is an effective target for the treatment of PTC patients.
This paper investigates the air–ground cooperative time-varying formation-tracking control problem of a heterogeneous cluster system composed of an unmanned ground vehicle (UGV) and an unmanned ...aerial vehicle (UAV). Initially, the structure of the UAV–UGV formation-control system is analyzed from the perspective of a cooperative combat system. Next, based on the motion relationship between the UAV–UGV in a relative coordinate system, the relative motion model between them is established, which can clearly reveal the physical meaning of the relative motion process in the UAV–UGV system. Then, under the premise that the control system of the UAG is closed-loop stable, the motion state of the UGV is modeled as an input perturbation. Finally, using a linear quadratic optimal control theory, a UAV–UGV formation-maintenance controller is designed to track the reference trajectory of the UGV based on the UAV–UGV relative motion model. The simulation results demonstrate that the proposed controller can overcome input perturbations, model-constant perturbations, and linearization biases. Moreover, it can achieve fast and stable adjustment and maintenance control of the desired UAV–UGV formation proposed by the cooperative combat mission planning system.
According to our experience, the typical phenotypes, such as special facial features or multiple congenital anomalies (MCAs), indicate a high risk of genetic disease and lead physicians to perform ...genetic testing in neonates as early as possible. ...in practice, infants without typical phenotypes typically undergo a long and costly diagnostic process before genetic diagnoses are confirmed. ...a recent survey by the American College of Medical Genetics and Genomics (ACMG) and other national professional organizations indicated that there are insufficient numbers of qualified geneticists to fulfil genetic service needs. 5 However, to our knowledge, clinical findings are variable and documented based on the physician's experience and training. ...the massive expressions of clinical findings hamper the analysis of character phenotypes in genetic diseases. ...in addition to structural malformations and special facial features, these three phenotypes could be considered as the clinical indicators to perform further genetic testing.
A distributed fuzzy adaptive control with similar parameters is constructed for a class of heterogeneous multiagent systems. Unlike many existing works, the dimensions of each multiagent dynamic ...system are considered to be nonidentical in this paper. Firstly, similar properties for different dimensions of multiagent systems are introduced, and some similar parameters among multiagent systems are also proposed. Secondly, a distributed fuzzy adaptive control on the basis of similar parameters is designed for the consensus of leader-follower multiagent systems. Following the graph theory and Lyapunov stability approach, it is concluded that UUB (uniformly ultimately bounded) of all signals in the closed-loop system can be guaranteed, and the consensus tacking error converges to a small compact zero set. Finally, a simulation example with different dimensions is provided to illustrate the effectiveness of the proposed method.
The explosion of next-generation sequencing (NGS) has enabled the widespread use of genomic data in precision medicine. Currently, several neonatal genome projects have emerged to explore the ...advantages of NGS to diagnose or screen for rare genetic disorders. These projects have made remarkable achievements, but still the genome data could be further explored with the assistance of phenotype collection. In contrast, longitudinal birth cohorts are great examples to record and apply phenotypic information in clinical studies starting at the neonatal period, especially the trajectory analyses for health development or disease progression. It is obvious that efficient integration of genotype and phenotype benefits not only the clinical management of rare genetic disorders but also the risk assessment of complex diseases. Here, we first summarize the recent neonatal genome projects as well as some longitudinal birth cohorts. Then, we propose two simplified strategies by integrating genotypic and phenotypic information in precision medicine based on current studies. Finally, research collaborations, sociological issues, and future perspectives are discussed. How to maximize neonatal genomic information to benefit the pediatric population remains an area in need of more research and effort.
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In recent years, rainstorm chain disasters occurred frequently in megacities in China, seriously threatening people’s life and property safety and social stability. Therefore, it is essential to take ...effective measures to timely break the chain and mitigate the disasters.This study thoroughly analyzes the disaster chain system of rainstorms and studies the trend deduction model of waterlogging disasters and the important role of various deductive simulation results in intercepting the disaster chain. It also proposes a chain breaking idea of combining simulation deduction with comprehensive response and clarifies the response strategies of various emergency bodies in the disaster response in megacities. Moreover, an overall response idea based on the policy of preparation, supervision, simulation, connection, and rescue is proposed. In view of the key problems including multi-department plan coordination, multi-dimensional information aggregation, multi-level accurate warning, and multi-team linked dispatching, we propose the following countermeasures: completing the comprehensive plan system, constructing a monitoring and early warning network, improving simulation and deduction application, strengthening joint response ability, and boosting the accurate rescue ability. Furthermore, development suggestions are proposed from the following aspects: formulation of policies and regulations, compilation of standards, application of intelligent technology, promotion of intelligent early warning and directional release technology, and formation of a new pattern of “national emergency.”
We aimed to investigate the clinical and genetic risk factors associated with neonatal severe unconjugated hyperbilirubinemia.
This was a retrospective, 1:1 matched, case-control study. We included ...614 neonates diagnosed with severe unconjugated hyperbilirubinemia (serum total bilirubin level ≥425 μmol/L or serum total bilirubin concentration that met exchange transfusion criteria) from the China Neonatal Genomes Project in Children's Hospital of Fudan University. Clinical exome sequencing data were analyzed using a data analysis pipeline of Children's Hospital of Fudan University. The factors associated with severe unconjugated hyperbilirubinemia were assessed using univariable and multivariable logistic regression analyses. Interaction analyses were examined between clinical and genetic risk factors.
ABO/Rh incompatibility hemolysis (odds ratio OR 3.36, 95% confidence interval CI 2.32-4.86), extravascular hemorrhage (OR 2.95, 95% CI 2.24-3.89), weight loss (OR 5.46, 95% CI 2.88-10.36), exclusive breastmilk feeding (OR 3.56, 95% CI 2.71-4.68), and the homozygous mutant of UGT1A1 211G>A (OR 2.35, 95% CI 1.54-3.59) were all identified as factors significantly associated with severe unconjugated hyperbilirubinemia. The presence of UGT1A1 211G>A mildly increased the risk of severe unconjugated hyperbilirubinemia caused by ABO/Rh incompatibility hemolysis (OR 3.98, 95% CI 2.19-7.23), although the effect is not statistically significant.
ABO/Rh incompatibility hemolysis, extravascular hemorrhage, weight loss, exclusive breastmilk feeding, and the homozygous mutant of UGT1A1 211G>A were found to be risk factors for severe unconjugated hyperbilirubinemia. Clinical factors remain the most crucial and preventable determinants in managing severe unconjugated hyperbilirubinemia, with a minimal genetic contribution. The establishment of preconception care practices and the reinforcement of screening for the aforementioned risk factors are essential steps for preventing severe unconjugated hyperbilirubinemia.
Ankylosing spondylitis (AS) is a progressive systemic disease characterized by a chronic inflammatory response in the sacroiliac joints and spine. Long noncoding RNAs suggest significant actions in ...the progression of AS. Therefore, a specific lncRNA, highly upregulated in liver cancer (HULC), was studied here regarding its functions and related mechanisms in AS.
Measurements of miR-556-5p, HULC, and YAP1 expression were performed on AS cartilage tissues and chondrocytes. The interaction between miR-556-5p and HULC or YAP1 was verified. CCK-8, flow cytometry and enzyme-linked immunosorbent assay were used to evaluate the effects of HULC, miR-556-5p, and YAP1 on the proliferation, apoptosis, and inflammatory response of AS chondrocytes. Furthermore, the action of HULC/miR-556-5p/YAP1 was experimentally observed in AS mice.
HULC and YAP1 levels were augmented, while miR-556-5p levels were suppressed in AS cartilage tissues and chondrocytes. Downregulating HULC or upregulating miR-556-5p stimulated chondrocyte proliferation and inhibited apoptosis and inflammation in AS. miR-556-5p was a downstream factor of HULC, and YAP1 was a potential target of miR-556-5p. The improvement effect of downregulated HULC on AS chondrocytes was saved when YAP1 expression was forced. In addition, silence of HULC improved the pathological injury of spinal cartilage in AS mice by enhancing miR-556-5p-related regulation of YAP1.
HULC inhibition relieves the inflammatory response in AS by reducing miR-556-5p-mediated YAP1 expression.
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