The purpose of this study was to determine intraobserver, interobserver, and inter-day reliability levels for stiffness measurements of the patellar tendon and rectus femoris muscle using shear wave ...elastography (SWE).
This study was conducted on 12 healthy male individuals. Two examiners measured mean shear wave velocity values of the patellar tendons and rectus femoris muscles of both extremities using a 9L4 (4-9 MHz) transducer and an Acuson S3000 ultrasound system (Siemens Medical Solutions, Mountain View, CA). The elasticity images were acquired by the Virtual Touch tissue imaging quantification technique (Siemens Medical Solutions). Measurements were repeated 20 minutes and 1 week after the first measurements. The reliability of SWE measurements was assessed by means of the intraclass correlation coefficient (ICC).
The 12 participants ranged in age from 19 to 33 years (mean age ± SD, 25.33 ± 4.56 years). For the patellar tendon stiffness measurements with SWE, it was found that intraobserver reliability (ICC, 0.91-0.92) and interday reliability (ICC, 0.81-0.83) were excellent, and interobserver reliability (ICC, 0.71) was good. For the rectus femoris muscle stiffness measurements with SWE, it was found that the intraobserver reliability (ICC, 0.93-0.94), interday reliability (ICC, 0.81-0.91), and interobserver reliability (ICC, 0.95) were perfect.
Shear wave elastography using the Virtual Touch tissue imaging quantification technique is a reliable and repeatable technique for patellar tendon and rectus femoris stiffness measurements according to intraobserver, interday, and interobserver ICC values.
This study was conducted to investigate the protective effects of lycopene against the toxic effects of Aflatoxin B
(AFB
) exposure in kidney and heart of rat by evaluating antioxidant defense ...systems and lipid peroxidation (LPO). Forty-two healthy three-month-old male Wistar-Albino rats were used in this study. The animals were randomly divided into six experimental groups including 7 rats in each. These groups were arranged as follows: control group, lycopene (5 mg/kg/day, orally for 15 days) group, AFB
(0.5 mg/kg/day, orally for 7 days) group, AFB
(1.5 mg/kg/day, orally for 3 days) group, AFB
(0.5 mg/kg/day, orally for 7 days) + lycopene (5 mg/kg/day, orally for 15 days) group and AFB
(1.5 mg/kg/day, orally for 3 days) + lycopene (5 mg/kg/day, orally for 15 days) group. The animals were sacrificed at the end of applications. In this study, malondialdehyde (MDA) levels significantly increased; while reduced glutathione (GSH), glutathione-S-transferase (GST), catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and glucose-6-phosphate-dehydrogenase (G6PD) activities decreased in kidney and heart tissues. The significant reduction in the activities of antioxidant enzymes and non-enzymatic antioxidant system in AF treated rats as compared to the control group could be responsible for increased MDA levels observed during AF induced kidney and heart damage. The results showed increased urea, creatinine levels, as well as reduction sodium concentrations in plasma of AFB
treated rats. There was lycopene showed protection against AF induced nephrotoxicity and cardiotoxicity.
Can HCG MoM Ratio Predict Preeclampsia? TOKGÖZ, Betül; KIZILBOĞA, Gizem; YILMAZ ERGANİ, Seval ...
Hitit medical journal,
02/2024, Volume:
6, Issue:
1
Journal Article
Peer reviewed
Open access
Objective: To predict preeclampsia by the ratio of the HCG MoM value in the first and second trimester screening tests. Material and Methods: The HCG-MoM values of 136 pregnant women with ...preeclampsia and 222 normatensive pregnant women used for first and second-trimester screening tests were proportioned , and the difference between the groups was examined. Numeric variables are expressed as mean ± standard deviation, median (minimum – maximum), and categorical variables as n (%). Results: HCG MoM values were not different between the two groups and were within the accepted international values. The ratio of the free HCG MoM value in the first trimester to the intact HCG MoM value in the second trimester was significantly higher in the control group (1.06) than in the study group (0.99) (p=0.02). Conclusion: Rating the MoM values of β-HCG, a biochemical marker used in screening tests for chromosomal anomaly, may predict preeclampsia in the later weeks of pregnancy.
Amaç: Birinci ve ikinci trimester tarama testlerindeki HCG MoM değerinin oranına göre preeklampsiyi öngörmek. Gereç ve Yöntemler: 136 preeklampsili gebe ile 222 normal tansiyonlu gebenin birinci ve ikinci trimester tarama testleri için kullandıkları HCG-MoM değerleri oranlanarak gruplar arasındaki fark incelendi. Sayısal değişkenler ortalama ± standart sapma, medyan (minimum – maksimum), kategorik değişkenler ise n (%) olarak ifade edilmiştir. Bulgular: İki grup arasında HCG MoM değerleri arasında fark izlenmedi ve kabul edilen uluslararası değerler içerisindeydi . Birinci trimesterdeki serbest HCG MoM değerinin, ikinci trimesterdeki intakt HCG MoM değerine oranı kontrol grubunda (1,06) çalışma grubuna göre (0,99) anlamlı olarak yüksekti (p=0,02). Sonuç: Kromozomal anomali tarama testlerinde kullanılan biyokimyasal bir belirteç olan β-HCG'nin MoM değerlerinin derecelendirilmesi, gebeliğin ileri haftalarında preeklampsiyi öngörebilir.
Melatonin is a component of the antioxidant defense system since it has radical scavenging and antioxidant activities. In the present study, we aimed to investigate the endogenous rhythm of ...antioxidant enzyme glutathione peroxidase (GSH-Px) activity, oxidized glutathione (GSSG) and lipid peroxidation levels in tissues of pinealectomized rats (PINX). Rats were sacrificed by decapitation at 4 h intervals. GSH-Px activity, GSSG and lipid peroxidation levels showed a daily rhythm both in controls and in PINX rats. GSH-Px and GSSG exhibited the peak levels after the peak time of melatonin which was determined previously by other groups. Lipid peroxidation levels increased progressively during the night and started to decline before the GSH-Px peak time. These findings suggest that endogenous melatonin is involved in the night time increase of GSH-Px activity and GSSG levels and modulates the daily rhythm pattern of GSH-Px. In conclusion, pinealectomy which eliminates the melatonin rhythm has a supressor effect on GSH-Px activity levels.
OBJECTIVE: Anxiety and depression are associated with the interaction of many social factors, but pregnancy and puerperium are periods when patients are particularly sensitive to depression and ...anxiety. Assessing the level of anxiety and depression in high-risk pregnancies during the pandemic process and their attitudes toward vaccination.
STUDY DESIGN: In a case-control study, the Beck Depression and Beck Anxiety Scales were administered to 62 high-risk pregnant women and 296 normal pregnant women who registered at our hospital. At the end of the entire quarantine period and the use of the vaccine in our country, all patients were called and asked whether they had been vaccinated.
RESULTS: Anxiety and depression scores of normal patients were higher than those of patients in the risk group (p=0.002 and p<0.001, respectively). The anxiety and depression scores of the normal pregnant patients who were treated as outpatients were higher than those of the patients who were treated as inpatients (p=0.003 and p<0.001, respectively). Depression scores of patients in the last trimester of pregnancy were higher than those of patients at a smaller gestational age (p=0.009). Looking back to the pandemic period after vaccine discovery, depression scores of high-risk pregnant women were found to be higher and those who had a normal pregnancy had higher anxiety and depression scores (p=0.002, p<0.001, p<0.001).CONCLUSIONS: Hospitalization had different effects on high-risk and normal pregnant women; while hospitalization increased anxiety in high-risk pregnancies, outpatient follow-up increased anxiety and depression in the normal group.
Oxidative damage caused by aflatoxin (AF) in rat liver tissue and the inhibition effect of lycopene against this injury was investigated. Groups were formed as; control group (not treated), lycopene ...group (5 mg/kg/day, gavage for 15 days), AFB1 group (0.5 mg/kg/day, gavage for 7 days) and AFB1 + lycopene group. Lycopene administered simultaneously with AFB1. It was observed significant increase in malondialdehyde level, decrease in glutathione level, antioxidant enzyme activities in liver tissue of AFB1 group when compared with control group. It was determined to significantly increase in plasma aspartate transaminase, alanine transaminase, lactate dehydrogenase activities in AFB1 group when compared with control group. It was determined significant decrease in malondialdehyde level, plasma aspartate transaminase, alanine transaminase, lactate dehydrogenase activities and increase in glutathione level, antioxidant enzyme activities in AFB1 + lycopene group when compared with AFB1 group. This study suggests that lycopene which has antioxidant properties can be prevented from AFB1 induced hepatotoxicity.
The present study investigated the effects of propolis and vitamin E supplementation in diets of juvenil rainbow trout subjected to two different flow rates with or without flow stress (0.9 and 2.1 ...l/min, respectively) on final weigth (FW), condition factor (CF), feed conservation ratio (FCR), protein efficiency ratio (PER) and vitamin A, C and E concentrations and malondialdehyde (MDA) levels in serum as well as plasma superoxide dismutase (SOD) activity. The experimental groups were as follows: group C was fed a basal diet, group P10 was fed a basal diet supplemented with 10 g propolis/kg, group P30 was fed a basal diet supplemented with 30 g propolis/kg, group E60 was fed a basal diet supplemented with 60 mg vitamin E/kg. CF and PER were not different among all diets groups for both flow rate treatments (p > 0.05). The FCR improved in P10, P30 and E60 diet groups compared to C diet group at 2.1 l/min flow rate (p < 0.05). The FCR of E60 and P30 were found to be better than C and P10 diet groups at 0.9 l/min flow rate (p < 0.05). Vitamin E and propolis supplementations did not affect vitamin A concentration of serum for either flow rate (p > 0.05). Fish fed with diet E60had higher serum vitamin E concentration than other groups (p < 0.05). Serum Vitamin C concentration was only affected by the propolis supplemented diets. MDA level of E60 group was found significantly lower comperad to other groups for both flow rate (p < 0.05). Plasma SOD activity significantly decreased in the E60 group compared to other groups at 2.1 l/min flow rate (p < 0.05). But plasma SOD activity was not different among all diets groups at 0.9 l/min flow rate (p > 0.05). PUBLICATION ABSTRACT
The aim of this study was to evaluate potential protective effects of propolis on furan-induced hepatic damage by assessing the levels of malondialdehyde (MDA) and reduced glutathione (GSH), ...antioxidant enzyme activities, and histopathological changes in the liver.
Albino Wistar rats were divided into six groups: a control, propolis-treated (100 mg/kg b.w./day), low-dose furan-treated (furan-L group; 2 mg/kg b.w./day), high-dose furan-treated (furan-H group; 16 mg/kg b.w./day), furan-L+propolis treated, and furan-H+propolis treated group. Propolis and furan were applied by gavage; propolis for 8 days, and furan for 20 days in furan-L groups and 10 days in furan-H groups.
While MDA levels were elevated in furan-treated groups, levels of GSH and activities of antioxidant enzymes decreased (p < 0.001). The levels of MDA and GSH and activities of antioxidant enzymes were normal in the furan+propolis groups, especially in the furan-L+propolis group (p < 0.001). While the aspartate transaminase, alanine transaminase, alkaline phosphatase, and lactate pdehydrogenase activities were elevated in the furan-H treated group (p < 0.05 and p < 0.001), they were unchanged in the furan-L treated group. Histopathologically, several lesions were observed in the liver tissues of the furan-treated groups, especially in the higher-dose group. It was determined that these changes were milder in both of the furan+propolis groups.
The results indicate that propolis exhibits good hepatoprotective and antioxidant potential against furan-induced hepatocellular damage in rats.
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