The diagnosis of TAPVC in the prenatal period, especially if it is in the obstructive form, is very important in terms of both delivery planning and postnatal management. Here we show how a rare ...obstructive mixed type abnormal pulmonary venous connection is diagnosed prenatally.
This study aims to investigate the effects of Ozone therapy used in traditional and complementary medicine on possible toxicity caused by Acetaminophen (APAP). There was no intervention in first ...group (control). The second group received 150 µg·kg-1 day, i.p. for three weeks of ozone, 2 g·kg-1 orally single dose APAP to third group, and APAP+Ozone to fourth group. APAP was administered on 21st day of ozone application. Malondialdehyde (MDA) levels, reduced glutathione (GSH) levels, and antioxidant enzyme activities were all measured to assess their contribution to pathogenesis of toxicity in blood tissues. Compared to the control group, the group receiving APAP showed increased MDA levels (P=0.009) and decreased GSH levels (P<0.001), as well as reduced CAT (P<0.001), GSH–Px (P<0.001) and SOD (P<0.001) enzyme activities. However, in the group treated with ozone and APAP, levels of MDA and GSH, as well as the activities of the antioxidant enzymes, were similar to those of the control group, indicating a protective effect of ozone against APAP–induced oxidative stress. In conclusion, the results of the study showed that APAP caused oxidative stress in blood tissue. The present study showed that ozone had potential protective effects against toxicity induced with APAP through various mechanisms in different cellular processes. This may be related to cytoprotective and antioxidant properties of ozone. Ozone can provide a chemical basis for some health benefits against toxicities. Ozone may be protective against APAP–induced oxidative damage. As a result, it was concluded that ozone may be a natural and effective antioxidant that can be used to reduce the toxicity caused by APAP.
This study aimed to investigate the effect of propolis on pyruvate kinase (PK) which is a key enzyme in glycolysis and superoxide dismutase (SOD), an antioxidant enzyme on toxicity induced by DOX in ...different tissues. Using molecular docking, It was looked into how propolis affected the enzymes responsible for glycolysis and the antioxidant system. There was no application in the first group (control). The second group received 100 mg·kg-1 day of propolis by gavage needle for 7 days, a single dose of 20 mg·kg-1 intraperitoneal DOX to the third group, and propolis+DOX to the fourth group. Two days prior to DOX administration, propolis application began, and it lasted for seven days. PK and SOD activities were determined in liver, heart, kidney, and testis tissues, and molecular docking was applied to ratify the activity of some propolis components (caffeic acid phenethyl ester (CAPE) and Quercetin) on PK and SOD enzymes. When the DOX group was compared with the control group, a decrease in PK and SOD activities were found, and significant difference was found in PK and SOD activities. Administration of DOX decreased PK and SOD activities of liver, heart, kidney, and testis tissues. In conclusion, our study reveals that DOX disrupts glycolysis in rat tissues. CAPE and Quercetin compounds were shown to interact similarly with the cocrystal ligands of PK and SOD. In addition, when the interaction types of these compounds especially on PK and the docking scores obtained were examined, it can be said that they show higher affinity than DOX.
The aim of this study was to investigate the effects of lycopene on cisplatin-induced nephrotoxicity and oxidative stress in rats. Adult male Sprague–Dawley rats were randomly divided into four ...groups. The control group (group 1) received physiological saline; animals in group 2 received only cisplatin; a 10 days of lycopene pre-treatment was applied to the animals in group 3 before administration of cisplatin; a 5 days of lycopene treatment was performed following administration of cisplatin for the animals in group 4. Cisplatin (7
mg/kg) was intraperitoneally injected as a single dose and lycopene (4
mg/kg) was administered by gavage in corn oil. Biochemical and histopathological methods were utilised for evaluation of the nephrotoxicity. The concentrations of creatinine, urea, Na+ and K+ in plasma and levels of malondialdehyde and reduced glutathione as well as glutathione peroxidase and catalase activities were determined in kidney tissue. Administration of cisplatin to rats induced a marked renal failure, characterized with a significant increase in plasma creatinine and urea concentrations. Na
+ and K
+ levels of rats received cisplatin alone were not significantly different compared to control group, but they had higher kidney malondialdehyde, and lower reduce glutathione concentrations, glutathione peroxidase and catalase activities. Lycopene administration produced amelioration in biochemical indices of nephrotoxicity in both plasma and kidney tissues when compared to group 2; pre-treatment with lycopene being more effective. Results from this study indicate that the novel natural antioxidant lycopene might have protective effect against cisplatin-induced nephrotoxicity and oxidative stress in rat.
The study determined the damage caused by formaldehyde (FA) exposure in blood and liver samples using biochemical markers. Histopathological analysis was performed using the terminal deoxynucleotidyl ...transferase dUTP nick end labeling (TUNEL) method and measurement of CD68 cell density. To what extent the antioxidant molecules thymoquinone (TQ) and ozone (O
) reversed the damage caused by FA exposure was investigated, both when used alone and combined.
Fifty-six Sprague-Dawley male rats of eight to ten weeks of age were used in the experiment. The rats were divided into eight groups, with seven rats in each group: the untreated control group, the group treated with TQ (10 mg/kg/day), the group treated with O
(150 μg/kg/day), the group treated with TQ+O
, the group exposed to FA (10 ppm 8 h/day), the group receiving FA+TQ, the group receiving FA+O
, and the group receiving FA+TQ+O
. Serum aspartate transaminase (AST), alanine transaminase (ALT), total antioxidant (TAS, U/mL), and total oxidant (TOS, nmol/mL) levels were analyzed. TAS and TOS levels, CD68 cell density, and apoptotic cells were determined in liver tissues.
FA exposure caused an increase in serum AST and ALT levels of (p<0.05) experimental animals, a decrease in TAS levels in serum (p=0.03) and liver (p>0.05) and an increase in TOS levels (p>0.05), TUNEL positivity (p<0.001), and CD68 cell density (p=0.004). Administration of TQ and O
as antioxidants significantly reversed biochemical and histopathological alterations in the serum and liver.
TQ and ozone therapy suppressed oxidative stress caused by FA exposure and reversed the emerging histopathological deteriorations. Ozone therapy did not suppress the effects of TQ. Therefore, ozone therapy can be given as a supportive therapy along with the main therapeutic agents. We think TQ and ozone therapy may be useful to protect individuals exposed to FA.
The positive effects of grape seed proanthocyanidin extract (GSPE) on bone health, which is a potent antioxidant, are known but its effects on fracture healing are not sufficiently covered in the ...literature. This study aims to investigate the effects of GSPE on fracture healing and biomechanics of healing bone.
Sixty-four adult Wistar-Albino male rats were divided into 8 groups of 8 animals in each group. Osteotomy was performed to the right femurs of all groups except the negative control (G1) and positive control (G2) groups, and intramedullary Kirchner wire was used for fixation. GSPE was given to half of the rats (G2-G4-G6-G8) 100 mg/kg/day by oral gavage. The rats were sacrificed on the tenth (G3-G4), twentieth (G5-G6), and thirtieth (G1-G2-G7-G8) days, respectively, and histopathological, radiological, and biomechanical examinations were performed.
Histopathological examination of the specimens from the callus tissues revealed that bone healing was more prominent in the groups supplemented with GSPE (G4, G6, G8). There was a statistically significant improvement in radiological recovery scores and callus volumes in groups with GSPE. When biomechanical strengths were evaluated, it was found that GSPE increased bone strength not only in fracture groups but also in the positive control group (G2).
As a result, this study showed that GSPE, a potent anti-oxidant, had a positive effect on bone healing and improved mechanical strength of the healing bone.
To evaluate the incidence, the risk factors, and the treatment outcomes of Non-tubal ectopic pregnancies (NTEP) treated in a tertiary care center.
A total of 110 NTEP cases treated between 2014 and ...2019 were included in the retrospective study. The study cohort was divided into 6 groups according to the pregnancy localization: 87 cesarean scar pregnancies (CSPs), 7 ovarian pregnancies, 6 interstitial pregnancies, 4 rudimentary horn pregnancies, 4 abdominal pregnancies, and 2 cervical pregnancies. One woman rejected all treatment modalities. Demographic characteristics, treatment modalities, and outcomes of each group were evaluated.
In the study cohort, expectant management was performed in one (0.9%) woman. The methotrexate (MTX) treatment was administered in 29 (26.3%) women. Seventeen (15.4%) women underwent surgery, and 63 (57.2%) women underwent manual vacuum aspiration (MVA). A woman rejected all treatment modalities. Although 70.1% (n = 61) of CSPs were cured with MVA, 24.1% (n = 21) of them were treated with a single-dose MTX regimen in addition to MVA. The higher mean gestational sac size (33,9 ± 12,96 mm vs. 17,34 ± 9,87 mm), the higher mean gestational week (8,43 ± 1,16w vs. 6,66 ± 1,49w), the presence of fetal heartbeat (FHB) (90.5% vs. 26,2%) and the history of pelvic ınflammatory disease (PID) (38.1% vs. 6,6%) were found in the CSPs with MVA treatment failure (p < 0.05).
The management of NTEPs should be individualized according to the clinical and ultrasonographic findings. The size of the ectopic pregnancy mass, the gestational week, the presence of FHB, and the PID history were the predictive factors for the failure of MVA in CSP cases.
Çalışmada, kanser ve malignant olmayan hastalıkların tedavisinde etkili olduğu kanıtlanmış geniş klinik kullanımlı alkilleyici kemoterapötik bir ilaç olan siklofosfamid (CP)’in kardiyotoksisitesi ...üzerine propolisin etkileri incelenmiştir. Ratlar; kontrol grubu, propolis uygulanan grup (200 mg/kg/gün gavaj, 7 gün), CP uygulanan grup (150 mg/kg i.p. tek doz) ve CP+propolis uygulanan grup şeklinde 4 gruba ayrılmıştır. Propolis uygulamasına CP uygulamasından 2 gün önce başlanmış ve 7 gün süre ile devam edilmiştir. Kalp dokuları biyokimyasal ve histopatolojik incelemeler için alınmıştır. Kalp dokusunda malondialdehid (MDA), redükte glutatyon (GSH) düzeyleri, katalaz (CAT), glutatyon peroksidaz (GSH-Px), glutatyon-S-transferaz (GST) ve süperoksit dismutaz (SOD) aktiviteleri tayini yapılmıştır. Histopatolojik inceleme için kalp dokusu örnekleri uygun yöntemlerle hazırlanıp Masson’nun Trichrome ve Hematoksilen-Eozin tekniği ile boyandıktan sonra incelenmiştir. CP uygulanan grupta MDA, GSH düzeyleri ve CAT aktivitesinde artış, GSH-Px, GST ve SOD aktivitelerinde düşüş saptanmıştır (p<0.05). CP+propolis uygulanan grupta MDA, GSH düzeyleri ve antioksidan enzim aktivitelerinin normal düzeylere ulaştığı gözlenmiştir. Propolis uygulanan grup ile CP+propolis uygulanan gruplarda istatistiksel olarak önemli bir değişiklik gözlenmemiştir. Histopatolojik incelemelerde kontrol grubunda kalp dokusuna ait kesitler normal yapıda gözlenmiştir. Propolis uygulanan grupta kalp dokusu kontrol grubu ile benzer yapı göstermiştir. CP uygulanan grupta kalp kası lifleri arasında bazı alanlarda hemoraji ve vasküler konjesyon gözlenmiştir. CP+propolis uygulaması yer yer vasküler konjesyona yol açmıştır. CP’e bağlı kardiyotoksisitenin patogenezinde oksidatif stresin rol oynayabileceği, propolisin CP’e bağlı yan etkileri azaltabileceği görülmektedir.
The renin-angiotensin-aldosterone system was shown to be activated in severe COVID-19 infection. We aimed to investigate the relationship between angiotensin converting enzyme (ACE) levels, ACE gene ...polymorphism, type 2 diabetes (T2DM), and hypertension (HT) and the prognosis of COVID-19 infection.
This cross-sectional study analyzed the clinical features of adult patients with SARS-CoV-2 infection. ACE gene analysis and ACE level measurements were performed. The patients were grouped according to ACE gene polymorphism (DD, ID or II), disease severity (mild, moderate, or severe), and the use of dipeptidyl peptidase-4 enzyme inhibitor (DPP4i), ACE-inhibitor (ACEi) or angiotensin receptor blocker (ARB). Intensive care unit (ICU) admissions and mortality were also recorded.
A total of 266 patients were enrolled. Gene analysis detected DD polymorphism in the ACE 1 gene in 32.7% (n = 87), ID in 51.5% (n = 137), and II in 15.8% (n = 42) of the patients. ACE gene polymorphisms were not associated with disease severity, ICU admission, or mortality. ACE levels were higher in patients who died (p = 0.004) or were admitted to the ICU (p<0.001) and in those with severe disease compared to cases with mild (p = 0.023) or moderate (p<0.001) disease. HT, T2DM, and ACEi/ARB or DPP4i use were not associated with mortality or ICU admission. ACE levels were similar in patients with or without HT (p = 0.374) and with HT using or not using ACEi/ARB (p = 0.999). They were also similar in patients with and without T2DM (p = 0.062) and in those with and without DPP4i treatment (p = 0.427). ACE level was a weak predictor of mortality but an important predictor of ICU admission. It predicted ICU admission in total (cutoff value >37.092 ng/mL, AUC: 0.775, p<0.001).
Our findings suggest that higher ACE levels, but not ACE gene polymorphism, ACEi/ARB or DPP4i use, were associated with the prognosis of COVID-19 infection. The presence of HT and T2DM and ACEi/ARB or DPP4i use were not associated with mortality or ICU admission.
OBJECTIVE: We aimed to determine the effect of uterine leiomyoma characteristics and cesarean myomectomy on maternal and perinatal outcomes. STUDY DESIGN: The study included patients with singleton ...pregnancies and uterine leiomyomas who had delivered at or beyond 24 weeks' gestation; without comorbidities, uterine anomalies, or fetal malformations. Data from 240 patients were studied between 2012 and 2022 in the perinatology clinic of a tertiary care center. Maternal and perinatal outcomes were obtained from medical records. RESULTS: Among the women with uterine leiomyomas, 21.7% were delivered vaginally and 78.3% via cesarean section. Myomectomy was performed in 150 out of 188 (79.8%) patients undergoing cesarean section. It was found that cases with leiomyomas ≥7 cm, compared to those with <4cm, had deliveries at earlier gestational weeks (36w6/7±2d vs. 37w+6/7±2d, p=0.018) and had lower newborn birthweight (2849.44±516.74 g vs. 3237.5±350.6 g, p<0.001), longer operation time (105.92±34.78 min vs. 68.21± 22.31 min, p<0.001) and a higher rate of neonatal intensive care unit requirement (48.1% vs. 13.7%, p<0.001). In cases with ≥2 leiomyomas compared to those with single leiomyomas, gestational age at birth was smaller and birthweight was lower (p<0.05). The amount of blood loss (969.66±427.21 mL vs. 738.15±337.2 mL and 553.84±366.46 mL), duration of surgery (83.23±29.56 min vs. 64.47±17.96 min) and transfusion requirements (36% vs. 5.3% and 28.8%) were higher in women who underwent myomectomy during cesarean section than in the other women undergoing only cesarean section or delivering vaginally (p<0.05). CONCLUSION: It was found that a leiomyoma size of ≥7 cm and number of ≥2 were both associated with earlier gestational age at birth and lower birth weight. Myomectomy performed during cesarean section increased the blood loss, duration of surgery, and the need for transfusion.
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