We present here the synthesis of a novel fluorescent actinide polyrotaxane compound URCP1 through the utilization of an end‐cutting pseudorotaxane precursor with only the cucurbit6uril (CB6) ...macrocyclic components acting as linking struts. The non‐coordinating guest motif in the obtained polyrotaxane, with increased freedom and structural flexibility, can display intriguing temperature‐triggered conformational variations inside the cavity of CB6, which was clearly evidenced by crystallographic snapshots at different temperatures. Notably, this observation of temperature‐triggered structural dynamics in URCP1 represents the first report of actinide polyrotaxane with such feature in solid‐state. Moreover, URCP1 has a high photoluminescence quantum yield (PLQY) of 49.8 %, comparable to other luminescent uranyl compounds, and can work as a fluorescent probe to selectively detect Fe3+ over other eight competing cations in aqueous solution, with the limit of detection being as low as 4.4×10−3 ppm.
A new fluorescent uranyl‐coordination polyrotaxane only connected by macrocyclic host has been constructed. The non‐coordinating guest (C7BPy) in the obtained polyrotaxane shows thermal displacement and dynamic conformational variations with temperature increasing from 150 to 300 K. Meanwhile, this polyrotaxene can selectively detect Fe3+ over other eight competing cations in aqueous solution, with the limit of Fe3+ detection as low as 4.4×10−3 ppm.
Acupuncture has been widely used in the treatment of knee osteoarthritis (KOA), but the selection of acupoints is indeterminate and lacks biological basis. The skin temperature of acupoints can ...reflect the state of local tissue and may be a potential factor for guiding acupoint selection. This study aims to compare the skin temperature of acupoints between KOA patients and the healthy population.
This is a protocol for a cross-sectional case-control study with 170 KOA patients and 170 age- and gender-matched healthy individuals. Diagnosed patients aged 45 to 70 will be recruited in the KOA group. Participants in the healthy group will be matched with the KOA group based on mean age and gender distribution. Skin temperature of 11 acupoints (ST35, EX-LE5, GB33, GB34, EX-LE2, ST34, ST36, GB39, BL40, SP9, SP10) will be extracted from infrared thermography (IRT) images of the lower limbs. Other measurements will include demographic data (gender, age, ethnicity, education, height, weight, BMI) and disease-related data (numerical rating scale, pain sites, duration of pain, pain descriptors, pain activities).
The results of this study will provide biological evidence for acupoint selection. This study is a precondition for follow-up studies, in which the value of optimized acupoint selection will be verified.
ChiCTR2200058867.
Display omitted
•s-ZVI-coupled Myriophyllum aquaticum-derived biochar favors C storage more than I.H.•Biochar nature or feedstock may control C trap and N removal gains from Fe-C coupling.•Biochars ...trigger C dynamics depending on trade-offs between priming and increased MBC.•Enhanced iron gate contributes to synergistic C sequestration and C sink augmentation.•β-glucosidase activity and Fe-OC increases jointly drive C cumulation in Fe-C ditches.
Carbon dioxide (CO2) is a more powerful greenhouse gas due to its enhanced instantaneous radiative forcing. Since agricultural ecological ditches (eco-ditches) controlling agricultural non-point source pollution are potential CO2 hotspots, carbon capture technologies must be deployed immediately to sequester carbon and combat climate change. Zero-waste biochar, a negative-carbon technology, remains contentious regarding C sequestration. Iron (Fe) enhances long-term organic carbon (OC) preservation, but the cascading effects of Fe-biochar interactions on the promotion of C accumulation are unclear in eco-ditches. In this paper, we fill these research gaps and employ sponge iron (s-ZVI) and biochar coupling (Fe-C) to enhance the iron gate C protection in eco-ditches. The results reveal that Myriophyllum aquaticum(M.A.)-derived biochar prepared by low-temperature pyrolysis (Biochar_M.A.) enjoys the highest FI, the lowest SUVA254 and SUVA280 and moderate dissolution stability. The coupling of s-ZVI and environmentally compatible Biochar_M.A. achieve a triple benefit situation of C sequestration, C sink, and pollutant removal. Overall carbon gain is jointly determined by abiotic controls and biotic controls. The carbon burial effects of biochar-amended eco-ditches are mainly controlled by the trade-offs between Fe-bound OC mineralization via the biochar-induced prime effect and preservation of OC derived from microbial biomass. The unique Fe-C boosts the rusty sink and yields a negative priming as a result of the dominance of Fe-OC. The upregulated β-glucosidase activity and new-formed Fe-OC indirectly and directly contribute to overall C gain in Fe-C-filled eco-ditches, respectively. These findings provide novel insights into the coupled biotic-abiotic contribution mechanisms underlying the iron gate and reverse enzyme latch phenomenon and advance more robust and prospective theoretical knowledge of C dynamics that are not restricted to eco-ditches, soil, constructed wetlands C storage.
A redox flow lithium-oxygen battery (RFLOB) by using soluble redox catalysts with good performance was demonstrated for large-scale energy storage. The new device enables the reversible formation and ...decomposition of Li2O2 via redox targeting reactions in a gas diffusion tank, spatially separated from the electrode, which obviates the passivation and pore clogging of the cathode.
Glutathione S-transferases (GSTs) play an active role in the development of drug resistance by numerous cancer cells, including melanoma cells, which is a major cause of chemotherapy failure. As part ...of our continuous effort to explore why dietary polyphenols bearing the catechol moiety (dietary catechols) show usually anticancer activity, catechol-type diphenylbutadiene (3,4-DHB) was selected as a model of dietary catechols to probe whether they work as pro-oxidative chemosensitizers via GST inhibition in melanoma cells. It was found that, in human melanoma A375 cells, 3,4-DHB is easily converted to its ortho-quinone via copper-containing tyrosinase-mediated two-electron oxidation along with generation of reactive oxygen species (ROS) derived from the oxidation; the resulting ortho-quinone and ROS are responsible for its ability to sensitize the cisplatin-resistant cells by inhibiting GST, followed by induction of apoptosis in an ASK1-JNK/p38 signaling cascade and mitochondria-dependent pathway. This work provides further evidence to support that dietary catechols exhibit antimelanoma activity by virtue of their tyrosinase-dependent pro-oxidative role and gives useful information for designing polyphenol-inspired GST inhibitors and sensitizers in chemotherapy against melanoma.
Objective
Oral lichen planus (OLP) is a T cell-mediated inflammatory condition in the oral cavity. Mucosal-associated invariant T (MAIT) cells are gaining more relevance in immune diseases because ...they can be activated by cytokines without T cell receptor stimulation. Herein, we tested the effect of interleukin-23 (IL-23) on the activation status of OLP MAIT cells.
Methods
Peripheral blood mononuclear cells (PBMCs) isolated from OLP patients were stimulated by IL-23 in the absence or presence of phorbol myristate acetate (PMA) and ionomycin. The activation status of MAIT cells was analyzed by flow cytometry after staining for CD3, CD4, CD8, CD161, TCR Vα7.2, and CD69.
Results
The fraction of MAIT cells in OLP peripheral blood was approximately 0.38% to 3.97%, and CD8+ subpopulations overwhelmed CD4+ cells. The mean percentages of OLP MAIT cells in PBMCs and CD8+MAIT cells in MAIT cells were approximately 40%. PMA and ionomycin significantly increased CD69 expression on OLP T cells, MAIT cells, and CD8+MAIT cells. Cells with enhanced activation had different responsiveness to exogenous IL-23, showing increased CD69 expression on OLP T cells, decreased CD69 on OLP CD8+MAIT cells, and no significant change on OLP MAIT cells.
Conclusions
IL-23 showed different effects on the activation status of OLP MAIT cells and CD8+MAIT cells.
The coal seam bifurcation area (CSBA) exists widely in coal measure strata, where the geological weak surface (GWS) slip in overburden structure is easy to induce coal burst. The coal mass of coal ...face shows overall instability failure and high-speed throwing characteristics during the coal burst, seriously threatening the safe and efficient coal mine production. In order to understand the GWS-induced coal burst caused by the slip in CSBA and find the main controlling factors of GWS slip, the GWS slip criterion in CSBA was established based on the coal burst case analysis of overburden structure in CSBA of 1305 coal face (1305CF) in Zhaolou Coal Mine. The case study showed that the angle and range of CSBA are the main controlling factors affecting GWS slip. The FLAC3D numerical model of CSBA was established to analyze the influence effect of main control factors. The results show that the increase of angle and range of CSBA will increase the influence scope and degree of coal face mining, improving coal face burst risk. However, the peak point region of abutment pressure will not be affected, gradually reaching its peak within 0 m ∼ 10 m from the coal seam merging area. With the increase of the angle of CSBA, the integrity of the triangular wedged rock mass along the GWS slip will be enhanced, aggravating the dynamic disturbance to the coal mass. With the increase of the range of CSBA, the slip of triangular wedged rock mass along GWS gradually changes from integral slip to phased slip, which will intermittently disturb the coal mass of the coal face. The research results have certain theoretical significance and practical value for preventing and controlling coal bursts in CSBA.
Glycolytic reprogramming is a typical feature of many cancers; however, key regulators of glucose metabolism reengineering are poorly understood, especially in cutaneous squamous cell carcinoma ...(cSCC). Here, Homeobox A9 (HOXA9), a direct target of onco-miR-365, is identified to be significantly downregulated in cSCC tumors and cell lines. HOXA9 acts as a tumor suppressor and inhibits glycolysis in cSCC in vitro and in vivo by negatively regulating HIF-1α and its downstream glycolytic regulators, HK2, GLUT1 and PDK1. Mechanistic studies show that HOXA9-CRIP2 interaction at glycolytic gene promoters impeds HIF-1α binding, repressing gene expression in trans. Our results reveal a miR-365-HOXA9-HIF-1α regulatory axis that contributes to the enhanced glycolysis in cSCC development and may represent an intervention target for cSCC therapy.
Kangaroo mother care (KMC) refers to the mother and baby after the birth of the early start of continuous skin contact way of a newborn care, which is a simple operation, easy controlled and with low ...cost, no large or high consumption of equipment.So it is very suitable for developing in areas where medical resources are relatively scarce, such as GuiZhou province where is a relatively poor province in China with many ethnic minorities.
This study selected the pregnant women who gave birth in Liping County, Guizhou Province, China, as the research object, to explore the impact of kangaroo mother care on the physiologic status of newborns in liping county, Guizhou Province.
A total of 347 hospitalized parturient women were divided into the KMC group and the control group. The results showed that the KMC group showed obvious advantages in stabilizing newborn vital signs, health indicators, promoting the success rate of breastfeeding and reducing newborn pain.
Research shows that kangaroo mother care is beneficial to postpartum maternal and infant health, and has advantages suitable for local characteristics, which is worth further promotion in minority areas of Guizhou Province.
Increasing demands for efficient and versatile chemical reactions have prompted innovations in enzyme engineering. A major challenge in engineering α-ketoglutarate-dependent oxygenases is to develop ...a rational strategy which can be widely used for directly evolving the desired mutant to generate new products. Herein, we report a strategy for rational redesign of a model enzyme, 4-hydroxyphenylpyruvate dioxygenase (HPPD), based on quantum mechanics/molecular mechanics (QM/MM) calculation and molecular dynamic simulations. This strategy enriched our understanding of the HPPD catalytic reaction pathway and led to the discovery of a series of HPPD mutants producing hydroxyphenylacetate (HPA) as the alternative product other than the native product homogentisate. The predicted HPPD–Fe(IV)O–HPA intermediate was further confirmed by the crystal structure of Arabidopsis thaliana HPPD/S267W complexed with HPA. These findings not only provide a good understanding of the structure–function relationship of HPPD but also demonstrate a generally applicable platform for the development of biocatalysts.
PDF
