•An electrostatic self-assembly strategy was proposed to prepare CQDs/HpCN nanocomposite.•Carbon quantum dots (CQDs) attached onto surface of proton-functionalized graphitic carbon nitride (HpCN) ...through electrostatic attraction.•The CQDs/HpCN nanocomposite exhibited excellent photocatalytic and photoelectrochemical properties.
Carbon quantum dots (CQDs) and graphitic carbon nitride (g-C3N4), as advanced metal-free material catalysts have been the focus of considerable attention because of their superior photocatalytic activities. In this study, we developed a novel approach to obtain CQDs/g-C3N4 nanocomposite with effective interfacial contact by incorporating negatively charged CQDs and tailor-made proton-functionalized g-C3N4via the electrostatic self-assembly strategy. Then, the morphology and microstructure of the new nanocomposite were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), ultraviolet–visible diffuse reflectance spectroscopy (DRS) and X-ray photoelectron spectroscopy (XPS). The CQDs and proton-functionalized g-C3N4 nanocomposite exhibited excellent electron transfer properties though electrochemical impedance spectroscopy (EIS), significantly enhanced photoactivity in the photoelectrochemical i–t curve test and degradation of methylene blue solution under visible light irradiation. These results demonstrated that the electrostatic self-assembly strategy process is a promising method of fabricating uniform metal-free material catalysts for an extensive range of applications.
In nanofluid minimum quantity lubrication (NMQL) milling of aviation aluminum alloy, it is the bottleneck problem to adjust the position parameters (target distance, incidence angle, and elevation ...angle) of the nozzle to improve the surface roughness of milling, which has large and uncontrollable errors. In this paper, the influence law of milling cutter speed, helical angle, and cavity shape on the flow field around the milling cutter was studied, and the optimal nozzle profile parameters were obtained. Using 7050 aluminum alloy as the workpiece material, the milling experiment of the NMQL cavity was conducted by utilizing cottonseed oil-based Al2O3 nanofluid. Results show that the high velocity of the surrounding air flow field and the strong gas barrier could be attributed to high rotating velocities of the milling cutter. The incidence angle of the nozzle was consistent with the helical angle of the milling cutter, the target distance was appropriate at 25–30 mm, and the elevation angle was suitable at 60°–65°. The range and variance analyses of the signal-to-noise ratio of milling force and roughness were performed, and the chip morphology was observed and analyzed. The results show that the optimal combination of nozzle position parameters was the target distance of 30 mm, the incidence angle of 35°, and the elevation angle of 60°. Among these parameters, target distance had the largest impact on cutting performance with a contribution rate of more than 55%, followed by incidence angle and elevation contribution rate. Analysis by orthogonal experiment revealed that the nozzle position parameters were appropriate, and Ra (0.087 μm) was reduced by 30.4% from the maximum value (0.125 μm). Moreover, Rsm (0.05 mm) was minimum, which was 36% lower than that of the seventh group (Rsm = 0.078 mm).
To solve the problem of low recognition accuracy and long recognition time in conventional methods for digital moving image feature recognition, a method for digital image feature recognition on ...mobile platforms based on machine learning is proposed. The support vector machine method is used to cluster the dataset, and then the weight reconstruction method is used to reduce the dimension of digital moving image data. The reduced dimension of the digital moving image is embedded in the coordinates to obtain the reduced dimension of the image data information and complete the feature recognition of the digital moving image. The simulation results show that the recognition accuracy of this method in recognizing features of moving digital images is basically stable at more than 95% and the running time is 0.42s. The recognition effect of the proposed method is good, the recognition efficiency is high, and it has some practical application value.
The factors regulating the heterogeneity of interleukin-17A (IL-17A)-expressing CD4
+
T cells in inflammatory bowel diseases remain unclear. In the current study, we characterised the expression and ...function of zinc finger protein 189 (ZFP189) in a murine colitis model. Mice were given dextran sulphate sodium to induce acute colitis. Flow cytometry was applied to recognise and enrich Th17 and Th17.1 cells based on the expression of IL-17A, interferon-γ (IFN-γ), C-X-C motif chemokine receptor 3 (CXCR3), and C-C motif chemokine receptor 4 (CCR4). The expression of ZFP189 in Th17 and Th17.1 cells was determined by Immunoblotting. Lentivirus-mediated ZFP189 knockdown was conducted to evaluate the effect of ZFP189 on the differentiation of Th17 and Th17.1 cells. The adoptive transfer was performed to analyse the pathogenicity of Th17.1 cells in vivo. We found that ZFP189 was mildly up-regulated in IL-17A-expressing CD4
+
T cells in colonic lamina propria. Lamina propria Th17.1 cells expressed higher ZFP189 than Th17 cells. In vitro ZFP189 knockdown in CD4
+
T cells did not impact Th17 cell differentiation but suppressed Th17.1 cell differentiation, as evidenced by lower T-box expressed in T cells (T-bet) and IFN-γ. When adoptively transferred into mice, ZFP189-deficient Th17.1 cells produced fewer IFN-γ, tumour necrosis factor-alpha (TNF-α), and granulocyte-macrophage colony-stimulating factor (GM-CSF) than ZFP189-expressing Th17.1 cells. Moreover, ZFP189-deficient Th17.1 cells induced less severe colitis than ZFP189-expressing Th17.1 cells, as evidenced by less body weight loss, a lower disease activity index, and a lower colon histological score. In summary, ZFP189 acts as a positive regulator of the differentiation and pathogenicity of lamina propria Th17.1 cells in colitis.
Key message
Due to high incidence of tuberculosis in Taiwan, there is a tendency for overdiagnosis of tuberculosis. Differential diagnosis between malignant diseases and pulmonary tuberculosis is ...extremely important for us.
We present a case of misdiagnosis of tuberculosis (TB). While TB is common in Taiwan, this case highlights the importance of differential diagnoses to avoid misdiagnosis and ensure appropriate treatment.
,
, and
are very closely related
species.
has long been proposed as a probiotic, whereas studies on functional characterization for
and
are some compared to
.
. In this study,
FBL6,
FBL7, and
FBL8 ...were isolated from raw milk, and their probiotic properties were investigated. Genomic analysis demonstrated the role of
and
as probiotic candidates. The three strains were tolerant to acid and bile salt, with inhibitory action against pathogenic bacterial strains and capacity of antioxidants. Complete genome sequences of the three strains were analyzed to highlight the probiotic properties at the genetic level, which results in the discovery of genes corresponding to phenotypic characterization. Moreover, genes known to confer probiotic characteristics were identified, including genes related to biosynthesis, defense machinery, adhesion, and stress adaptation. The comparative genomic analysis with other available genomes revealed 256, 214, and 32 unique genes for FBL6, FBL7, and FBL8, respectively. These genomes contained individual genes encoding proteins that are putatively involved in carbohydrate transport and metabolism, prokaryotic immune system for antiviral defense, and physiological control processes. In particular,
.
FBL6 had a bacteriocin gene cluster that was not present in other genomes of
.
, resulting in this strain may exhibit a wide range of antimicrobial activity compared to other
.
strains. Our data can help us understand the probiotic functionalities of the three strains and suggest that
and
species, which are closely related to
, can also be considered as novel potential probiotic candidate strains.
Background
HLA‐B*58:01 is a well‐known risk factor for allopurinol‐induced severe cutaneous adverse reactions (SCARs). However, only a minority of HLA‐B*58:01 carriers suffer SCARs after taking ...allopurinol. The aim of this study was to investigate subsidiary genetic markers that could identify those at further increased risk of developing allopurinol‐induced drug reaction with eosinophilia and systemic symptoms (DRESS) in subjects with HLA‐B*58:01.
Methods
Subjects with B*58:01 were enrolled (21 allopurinol‐induced DRESS and 52 allopurinol‐tolerant control). HLA‐A, ‐B, ‐C and ‐DRB1 alleles were compared. Comparison of risk between HLAs and allopurinol‐induced SCAR in separate populations was performed to support the results. Kruskal‐Wallis test, Pearson's chi‐square test, Fisher's exact test and binary logistic regression were used to analyze the risk of SCAR development.
Results
Frequencies of A*24:02 (71.4 vs. 17.3%, p < 0.001, odds ratio OR = 12.0; 95% confidence interval CI, 3.6–39.2) were significantly higher in B*58:01 (+) DRESS than B*58:01 (+) tolerant controls. In addition, DRB1*13:02 further increased the risk of DRESS. The phenotype frequency of A*24:02/DRB1*13:02 was significantly higher in the B*58:01 (+) DRESS group than in the B*58:01 (+) tolerant controls (52.4% vs. 5.8%, p < 0.001, OR, 66.0; 95% CI, 6.1–716.2). In 2782 allopurinol user cohort, the overall prevalence of DRESS was 0.22%, which increased to 1.62% and 2.86% in the presence of B*58:01 and B*58:01/A*24:02, respectively.
Conclusion
The additional secondary screening with A*24:02 and DRB1*13:02 alleles may identify those at further increased risk of allopurinol‐induced DRESS in B*58:01 carriers.
Concentric traveling ionosphere disturbances (CTIDs) in total electron content triggered by Super Typhoon Meranti on 13 September 2016 are detected by using the ground‐based Global Navigation ...Satellite Systems network in Taiwan. The CTIDs emanated outward before the typhoon landfall and lasted for more than 10 h. The characteristics of CTIDs agree with the gravity wave theory and exhibit spatial and temporal scales in wave periods of ~8–30 min, horizontal wavelengths of ~160–200 km, and horizontal phase velocities of ~106–220 m/s. We also observe the CTIDs showing the stationary wave character. Broad spectra of CTIDs are excited after the rainbands of typhoon impinged on Central Mountain Range of Taiwan. The ray‐tracing technique confirms that the CTIDs were excited by convective clouds, spiral rainbands, and the eyewall of Typhoon Meranti. This study provides new evidence of typhoon‐induced concentric gravity waves in the ionosphere.
Key Points
Concentric traveling ionosphere disturbances (CTIDs) in total electron content (TEC) are observed during Super Typhoon Meranti (2016)
The CTIDs agree with the gravity wave dispersion relation suggesting the upward propagation of typhoon‐induced gravity waves
Comparison between CTIDs and meteorological radar refractivity reveals multiple sources of excited waves
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