Urea, sodium dodecyl sulfate (SDS) and N-ethylmaleimide (NEM) were employed to monitor the contributions of hydrogen bonds, hydrophobic interactions and disulfide bonds in heat-induced surimi gel. ...Surimi gels were prepared with the bond disrupting agents followed by texture profile, gel strength, rheological and Raman analysis. The rheological analysis demonstrated that remarkable changes of storage moduli (G′) were presented below the critical temperature, especially for SDS treatments. Gel strength significantly decreased as the concentration of urea and NEM increased, while it was slightly improved by 1% SDS. With the involvement of bond disrupting agents, hardness and gumminess of surimi gel presented a dramatical decrease. The Raman results indicated that protein secondary structure tended to transform to random coil, with content of α-helix and β-sheet decreasing. Textural properties were in correlation with protein conformation. Less of random coil led to improvement of gel strength, and springiness was mainly contributed by β-sheet and β-turn. The results suggested that different bond disrupting agents induced various changes in surimi gel properties and protein secondary structure.
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•Urea, SDS and NEM impacted surimi gel properties by disrupting hydrogen bonds, hydropholic interactions and disulfide bonds.•Secondary structure of surimi gels was changed in the presence of bond disrupting agents. More random coil was formed accompany with decreasing content of β-sheet.•Textural properties were in correlation with protein secondary structure.
Hyperglycemia is generally considered to be an important cause of diabetic retinopathy (DR). The aim of the present study was to investigate the role of miR-5195-3p in high glucose (HG)-induced human ...retinal pigment epithelial ARPE-19 cell injury. Here, we first found that the expression level of miR-5195-3p was significantly downregulated in HG-stimulated ARPE-19 cells using reverse transcription quantitative PCR. Overexpression of miR-5195-3p attenuated the impaired cell viability, increased apoptosis and pro-inflammatory cytokines secretion in ARPE-19 cells under HG condition using CCK-8 assay, flow cytometry and ELISA assay, respectively. Luciferase reporter assay showed that miR-5195-3p could specifically bind to the 3'UTR of glia maturation factor-β (GMFB). GMFB overexpression reversed, while knockdown enhanced the protective effects of miR-5195-3p overexpression against HG-induced ARPE-19 cell injury. In summary, miR-5195-3p targeting GMFB might be a potential therapeutic target for DR.
In this paper, a novel joint sparse representation-based image fusion method is proposed. Since the sensors observe related phenomena, the source images are expected to possess common and innovation ...features. We use sparse coefficients as image features. The source image is represented with the common and innovation sparse coefficients by joint sparse representation. The sparse coefficients are consequently weighted by the mean absolute values of the innovation coefficients. Furthermore, since sparse representation has been significantly successful in the development of image denoising algorithms, our method can carry out image denoising and fusion simultaneously, while the images are corrupted by additive noise. Experiment results show that the performance of the proposed method is better than that of other methods in terms of several metrics, as well as in the visual quality.
•1% chitosan-hawthorn leaf extract coating notably delayed strawberries spoilage.•1% chitosan-hawthorn leaf extract coating bolstered antioxidative properties.•Chitosan-hawthorn leaf extract coating ...represents a valuable option to protect fruits.
Strawberries rapidly deteriorate postharvest, necessitating effective measures to extend their shelf life. This study focused on developing an eco-friendly chitosan-based protective film for strawberry preservation. Strawberries were treated with a coating solution containing varying concentrations of hawthorn leaf extract (HLE) (0.4%, 0.7%, and 1.0%), 1.5% chitosan (CH), and 1% acetic acid. The results demonstrated that coating strawberry fruit with 1% CH-HLE notably delayed fruit spoilage. In-depth analysis revealed that, compared with the uncoated strawberry fruits, the 1% CH-HLE coating effectively reduced weight loss, the respiration intensity, malondialdehyde (MDA) levels, and superoxide anion (O2·−) production. Additionally, the coated strawberries exhibited improved firmness, total soluble solids (TSS), vitamin C (Vc) content, titratable acidity (TA), and total phenolic compound (TPC) content. The enzyme activities of superoxide dismutase (SOD) and catalase (CAT) in the CH-HLE-coated strawberries were greater than those in their uncoated counterparts. The application of a 1% CH-HLE coating successfully delayed spoilage and extend the shelf life of the strawberries by approximately 4–5 days. These findings suggest that CH-HLE has significant potential as a resource for protecting fruits and vegetables, offering an environmentally sustainable solution for postharvest preservation.
Aging is the greatest risk factor for human diseases, as it results in cellular growth arrest, impaired tissue function and metabolism, ultimately impacting life span. Two different mechanisms are ...thought to be primary causes of aging. One is cumulative DNA damage induced by a perpetuating cycle of oxidative stress; the other is nutrient-sensing adenosine monophosphate-activated protein kinase (AMPK) and rapamycin (mTOR)/ ribosomal protein S6 (rpS6) pathways. As the main bioactive component of natural Chinese medicine rhizoma coptidis (
), berberine has recently been reported to expand life span in
and attenuate premature cellular senescence. Most components of
including berberine, coptisine, palmatine, and jatrorrhizine have been found to have beneficial effects on hyperlipidemia, hyperglycemia and hypertension aging-related diseases. The mechanism of these effects involves multiple cellular kinase and signaling pathways, including anti-oxidation, activation of AMPK signaling and its downstream targets, including mTOR/rpS6, Sirtuin1/ forkhead box transcription factor O3 (FOXO3), nuclear factor erythroid-2 related factor-2 (Nrf2), nicotinamide adenine dinucleotide (NAD
) and nuclear factor-κB (NF-κB) pathways. Most of these mechanisms converge on AMPK regulation on mitochondrial oxidative stress. Therefore, such evidence supports the possibility that rhizoma coptidis, in particular berberine, is a promising anti-aging natural product, and has pharmaceutical potential in combating aging-related diseases via anti-oxidation and AMPK cellular kinase activation.
Atherosclerosis (AS) is a life-threatening vascular disease. RNA N6-methyladenosine (m6A) modification level is dysregulated in multiple pathophysiologic processes including AS. In this text, the ...roles and molecular mechanisms of m6A writer METTL3 in AS progression were explored
and
. In the present study, cell proliferative, migratory, and tube formation capacities were assessed through CCK-8, Transwell migration, and tube formation assays, respectively. RNA m6A level was examined through a commercial kit. RNA and protein levels of genes were measured through RT-qPCR and western blot assays, respectively. VEGF secretion level was tested through ELISA assay. JAK2 mRNA stability was detected through actinomycin D assay. The relationship of METTL3, IGF2BP1, and JAK2 was investigated through bioinformatics analysis, MeRIP, RIP, RNA pull-down, and luciferase reporter assays. An AS mouse model was established to examine the effect of METTL3 knockdown on AS development
. The angiogenetic activity was examined through chick chorioallantoic membrane assay
. The results showed that METTL3 was highly expressed in ox-LDL-induced dysregulated HUVECs. METTL3 knockdown inhibited cell proliferation, migration, tube formation, and VEGF expression/secretion in ox-LDL-treated HUVECs, hampered AS process
, and prevented
angiogenesis of developing embryos. METTL3 positively regulated JAK2 expression and JAK2/STAT3 pathway in an m6A dependent manner in HUVECs. IGF2BP1 positively regulated JAK2 expression through directly binding to an m6A site within JAK2 mRNA in HUVECs. METTL3 knockdown weakened the interaction of JAK2 and IGF2BP1. METTL3 exerted its functions through JAK2/STAT3 pathway. In conclusion, METTL3 knockdown prevented AS progression by inhibiting JAK2/STAT3 pathway via IGF2BP1.
In the present paper, granular activated carbon (GAC) supported titanium dioxide (TiO
@GAC) particles were prepared by sol-gel process. Their performance in simultaneous adsorption of dye and Cu
from ...wastewater was studied. X-ray diffraction (XRD) indicated that TiO
of the TiO
@GAC microsphere is anatase type, and Fourier transform infrared spectroscopy (FT-IR) showed that the samples have obvious characteristic peaks in 400-800 cm
, which indicated that there are Ti-O-Ti bonds. The experimental results showed that the adsorption of TiO
@GAC for Methylene blue (MB) and Cu
were favorable under acidity condition, the adsorption of Methyl orange (MO) was favorable under alkalecent condition. The reaction kinetics of TiO
@GAC for MO, MB and Cu
were well described as pseudo-second-order kinetic model; The reaction isotherms for MO, MB and Cu
were well fitted by Langmuir model. The maximum adsorption capacity of TiO
@GAC for MO, MB and Cu
in the single systems were 32.36 mg/g, 25.32 mg/g and 23.42 mg/g, respectively. As for adsorption, Cu
had a suppression effect on MB, and a promotion effect on MO, however, the impact of MO and MB on Cu
were negligible.
Objective
The aim of this study was to investigate serum levels of soluble B-cell maturation antigen (sBCMA) in childhood-onset systemic lupus erythematous (cSLE) patients with renal involvement, and ...to elucidate their association with clinical characteristics.
Methods
116 cases of cSLE patients with renal involvement (84 females and 32 males; median age 11.6 (10.1, 12.9) years) hospitalized in Department of Pediatric Nephrology and Rheumatology, the First Affiliated Hospital, Sun Yat-sen University and 31 healthy controls (HCs) were enrolled. Serum concentrations of sBCMA were determined using enzyme-linked immunosorbent assay (ELISA). Clinical and laboratory information of cSLE patients were retrospectively analyzed.
Results
Serum sBCMA levels were significantly increased in primary cSLE when compared with treated cSLE patients and HCs, whereas there was no significant difference between treated cSLE patients and HCs. Patients with high disease activity displayed higher serum sBCMA levels compared with those with no or mild to moderate disease activity. Positive correlation was observed between serum sBCMA levels and systemic lupus erythematosus disease activity index-2K (SLEDAI-2K), antinuclear antibody titers, anti-double-stranded DNA titers, erythrocyte sedimentation rate, and immunoglobulin G levels, while sBCMA levels were negatively correlated with blood white blood cell count, hemoglobin, platelet count, complement C3 and C4 levels. Serum sBCMA levels decreased as disease ameliorated after treatments among 11 cases with follow-up examinations.
Conclusions
In cSLE patients with renal involvement, serum sBCMA levels correlated significantly with disease activity, immunological, and hematological parameters, but not with renal parameters. Our results suggest the potential and significance of serum sBCMA as a biomarker in cSLE patients.
This work investigated the effects of calcium chloride (CaCl
2
) as sodium replacement on the physicochemical properties of silver carp surimi. As NaCl replaced by 0.32% and 0.63% CaCl
2
, surimi ...gels presented better textural properties, breaking force and rheological properties, while 0.95%, 1.27% and 1.58% CaCl
2
led to the deterioration of gel strength. With the addition of CaCl
2
, water holding capacity (WHC) decreased significantly (p < .05), the T
23
relaxation time decreased and the proportion of T
23
increased. Compared with control, the sulphydryl content in surimi gel with 0.63% CaCl
2
increased by 23.61% and the solubility decreased by 17.31%. Furthermore, the Raman spectra data demonstrated that more β-sheet structures and random coils were induced in surimi gels prepared with CaCl
2
. Then surimi with and without CaCl
2
was used as food-ink for three-dimensional (3D) printing, it indicated that surimi with 0.32% and 0.63% CaCl
2
could effectively improve the 3D printability.
Type 2 diabetes mellitus (T2DM) is known to have obesity as a risk factor. Body mass index cannot distinguish between lean mass and fat mass. We aimed to examine the association between predicted fat ...mass, predicted lean mass, predicted percent fat and risk of T2DM in Japanese adults. We also explored whether these three new parameters could predict T2DM better than other obesity markers.
This present study is a secondary data analysis. The study enrolled 20,944 Japanese individuals who participated in the NAGALA medical assessment program between 2004 and 2015. 15,453 participants who are eligible and have complete information were included to our analysis. Through the use of Kaplan-Meier curve, restricted cubic spline and univariate and multivariate Cox regression analysis, the relationship between predicted fat mass, predicted lean mass, predicted percent fat and T2DM risk was examined. The area under the curve method was used to assess the differences between these markers of obesity.
A total of 373 cases of T2DM occurred over a median time of 5.4 years. In the male group, we found a U-shaped connection between predicted fat mass, predicted lean mass, and T2DM onset (p value, non-linearity < 0.05). A linear relationship was found between predicted percent fat and T2DM onset. The linear relationship was also found in the female group for predicted fat mass, and predicted percent fat. And for women, predicted lean mass was not an independent predictor. The area under the curve (AUC) for predicted fat mass, predicted lean mass, predicted percent fat in men was 0.673 (95%CI: 0.639 ~ 0.707), 0.598 (95%CI: 0.561 ~ 0.635), 0.715 (95%CI: 0.684 ~ 0.745), respectively. In males, WHtR was the strongest predictor (AUC 0.7151, 95%CI: 0.684 ~ 0.746), followed by predicted percent fat (AUC 0.7150, 95%CI: 0.684 ~ 0.745). In the females, WHtR was also the strongest predictor (AUC 0.758, 95%CI: 0.703 ~ 0.813), followed by body mass index (AUC 0.757, 95%CI: 0.704 ~ 0.811) and predicted percent fat (AUC 0.742, 95%CI: 0.687 ~ 0.798).
Predicted fat mass, predicted lean mass, predicted percent fat were strongly connected with an increased risk for developing T2DM in Japanese, particularly in males. WHtR and predicted percent fat had a slightly better discrimination than other common obesity indicators in males. In the females, predicted fat mass and predicted percent fat were associated with T2DM risk, WHtR and body mass index had the slightly higher predictive power.
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