Liver cancer accounts for 6% of all malignancies causing death worldwide, and hepatocellular carcinoma (HCC) is the most common histological type. HCC is a heterogeneous cancer, but how the tumour ...microenvironment (TME) of HCC contributes to the progression of HCC remains unclear. In this study, we investigated the immune microenvironment by multiomics analysis. The tumour immune infiltration characteristics of HCC were determined at the genomic, epigenetic, bulk transcriptome and single-cell levels by data from The Cancer Genome Atlas portal and the Gene Expression Omnibus (GEO). An epigenetic immune-related scoring system (EIRS) was developed to stratify patients with poor prognosis. SPP1, one gene in the EIRS system, was identified as an immune-related predictor of poor survival in HCC patients. Through receptor-ligand pair analysis in single-cell RNA-seq, SPP1 was indicated to mediate the crosstalk between HCC cells and macrophages via SPP1-CD44 and SPP1-PTGER4 association. In vitro experiments further validate SPP1 can trigger the polarization of macrophages to M2-phenotype tumour-associated macrophages (TAMs).
Changing film thickness to manipulate microstructural properties has been considered as a potential method in practical application. Here, we report that atomic-scale structural properties are ...regulated by film thickness in an NiCO2O4(NCO)/CuFe2O4(CFO) bilayer heterostructure prepared on (001)-MgAl2O4 (MAO) substrate by means of aberration-corrected scanning transmission electron microscopy (STEM). The misfit dislocations at the NCO/CFO interface and antiphase boundaries (APBs) bound to dislocations within the films are both found in NCO (40 nm)/CFO (40 nm)/MAO heterostructures, contributing to the relaxation of mismatch lattice strain. In addition, the non-overlapping a/4101-APB is found and the structural transformation of this kind of APB is resolved at the atomic scale. In contrast, only the interfacial dislocations form at the interface without the formation of APBs within the films in NCO (10 nm)/CFO (40 nm)/MAO heterostructures. Our results provide evidence that the formation of microstructural defects can be regulated by changing film thickness to tune the magnetic properties of epitaxial bilayer spinel oxide films.
Colorectal neuroendocrine carcinomas (CRNECs) are highly aggressive tumours with poor prognosis and low incidence. To date, the genomic landscape and molecular pathway alterations have not been ...elucidated.
Tissue sections and clinical information of CRNEC (n = 35) and CR neuroendocrine tumours (CRNETs) (n = 25) were collected as an in-house cohort (2010-2020). Comprehensive genomic and expression panels (AmoyDx® Master Panel) were applied to identify the genomic and genetic alterations of CRNEC. Through the depiction of the genomic landscape and transcriptome profile, we compared the difference between CRNEC and CRNET. Reverse transcription-polymerase chain reaction and immunofluorescence staining were performed to confirm the genetic alterations.
High tumour mutation load was observed in CRNEC compared with CRNET. CRNECs showed a "cold" immune landscape and increased endothelial cell activity compared with NETs. Importantly, PAX5 was aberrantly expressed in CRNEC and predicted a poor prognosis of CRNECs. CCL5, a factor that is considered an immunosuppressive factor in several tumour types, was strongly expressed in CRNEC patients with long-term survival and correlated with high CD8
T cell infiltration.
Through the depiction of the genomic landscape and transcriptome profile, we demonstrated alterations in molecular pathways and potential targets for immunotherapy in CRNEC.
Lanthanide metal-organic frameworks (Ln-MOFs) have attracted extensive attention because of their structural adjustability and wide optical function applications. However, MOFs with a wide linear pH ...response and stable framework structures in acidic or alkaline solutions are rare to date. Here, we used 4,4',4″-s-triazine-2,4,6-triyltribenzoate (H
TATB) as an organic ligand, coordinated with lanthanide ions (Eu
/Tb
), and synthesized a new metal-organic framework material. The material has a porous three-dimensional square framework structure and emits bright red or green fluorescence under 365 nm UV light. The carboxyl group of the ligand is prone to protonation in an acidic environment, and negatively charged OH
and ligand (TATB
) have a competitive effect in an alkaline environment, which could affect the coordination ability of ligand. The luminescence degree of the framework decreases with the increase in the degree of acid and base. In particular, such fluorescence changes have a wide linear response (pH = 0-14), which can be used as a potential fluorescence sensing material for pH detection.
Clarifying the electronic and magnetic properties of lutetium, lutetium dihydride, and lutetium oxide is very helpful to understand the emergent phenomena in lutetium-based compounds (such as ...superconductivity-like transitions near room temperature). However, this kind of study is still scarce at present. Here, we report on the electronic and magnetic properties of lutetium metals, lutetium dihydride powders, and lutetium oxide powders. Crystal structures and chemical compositions of these samples were characterized by x-ray diffraction and x-ray photoemission spectroscopy, respectively. Electrical transport measurements show that the resistance of lutetium has a linear behavior depending on temperature, whereas the resistance of lutetium dihydride powders changes little with decreasing the temperature. More interestingly, paramagnetism-ferromagnetism-spin glass transitions were observed at near 240 and 200 K, respectively, in lutetium metals. To understand their origins, the measurement of inductively coupled plasma optical emission spectroscopy was performed, revealing that the concentrations of dilute magnetic impurities in lutetium and lutetium dihydride are around 0.01%, which can lead to the presence of spin glassy behavior. Our work uncovers the complex magnetic properties of lutetium and lutetium dihydride and suggests their close connections to the electronic and magnetic transitions of Lutetium-based compounds (such as Lu–H–N).
The division of the territorial space functional area is the primary method to study the rational exploitation and use of land space. The research on the Production–Living–Ecological Space (PLES) ...change and its motivating factors has major implications for managing and optimizing spatial planning and may open up a new research direction for inquiries into environmental change on a global scale. In this study, the transfer matrix and landscape pattern index methods were used to analyze the temporal changes as well as the evolution features of the landscape pattern of the PLES in the Chaohu Lake Basin from 2000 to 2020. Using principal component analysis and grey correlation analysis, the primary driving indicators of the spatial changes of the PLES in the Chaohu Lake Basin and the degree of the influence of various driving factors on various spatial types were determined. The study concluded with a few findings. First, from the standpoint of landscape structure, the Chaohu Lake Basin’s agricultural production space (APS) makes up more than 60% of the total area, and it and urban living space (ULS) are the two most visible spatial categories. Second, the pattern of the landscape demonstrates that the area used for agricultural production holds a significant advantage within the overall structure of the landscape. Although there is less connectedness between different landscape types, less landscape dominance, and more landscape fragmentation, the structure of different landscape types tends to be more varied. Third, the findings of the driving analysis demonstrate that the natural climate, population structure of agricultural development, and industrial structure of economic development are the three driving indicators of the change of the PLES. Finally, in order to promote the formation of a territorial space development pattern with intensive and efficient production space, appropriate living space, and beautiful ecological space, it is proposed to carry out land regulation according to natural factors, economic development, national policies, and other actual conditions.
Based on the physical quantity of log data, the accurate identification of oil- and gas-bearing properties may be caused by the prestack inversion of fluid prediction, which will affect the success ...rate of exploration and development. Prestack data contain more information of amplitude and frequency. Using the frequency-dependent viscoelastic impedance equation and Bayesian inversion framework, the objective function of frequency-dependent elastic impedance inversion can be established to realize the frequency-dependent impedance inversion at different angles. According to the elastic impedance equation of the frequency-varying viscoelastic fluid factor, the relationship between elastic impedance and the frequency-dependent viscoelastic fluid factor is established, and the prestack seismic inversion method of the frequency-dependent viscoelastic fluid factor is studied. However, one of the important factors easily neglected is that we have been using logging data to establish fluid-sensitive parameters and the lithophysical version for fluid identification, so there are differences between logging and seismic frequency bands for fluid identification. The indicator factors with higher sensitivity to fluid can be selected by laboratory measurements. This article applies this method on Luojia oilfield data and verifies this method with log interpretation results, based on the sample of rock physics obtained in a low-frequency rock physics experiment; the technique of dispersion and fluid-sensitive parameters is studied, and the fluid prediction technology of a multifrequency band rock physics template is adopted, which can build the relationship between rock physical elastic parameters and fluid properties by the multifrequency broadband impedance method.
I3D-LSTM: A New Model for Human Action Recognition Wang, Xianyuan; Miao, Zhenjiang; Zhang, Ruyi ...
IOP conference series. Materials Science and Engineering,
07/2019, Volume:
569, Issue:
3
Journal Article
Peer reviewed
Open access
Action recognition has already been a heated research topic recently, which attempts to classify different human actions in videos. The current main-stream methods generally utilize ...ImageNet-pretrained model as features extractor, however it's not the optimal choice to pretrain a model for classifying videos on a huge still image dataset. What's more, very few works notice that 3D convolution neural network(3D CNN) is better for low-level spatial-temporal features extraction while recurrent neural network(RNN) is better for modelling high-level temporal feature sequences. Consequently, a novel model is proposed in our work to address the two problems mentioned above. First, we pretrain 3D CNN model on huge video action recognition dataset Kinetics to improve generality of the model. And then long short term memory(LSTM) is introduced to model the high-level temporal features produced by the Kinetics-pretrained 3D CNN model. Our experiments results show that the Kinetics-pretrained model can generally outperform ImageNet-pretrained model. And our proposed network finally achieve leading performance on UCF-101 dataset.
Abstract Background With the conflict between the promise of ageing in health and longevity and the limited availability of health resources and social support, older adults in China inevitably ...experience anxieties surrounding health risks. This study aims to investigate how older adults perceive the health risks that come with getting older, explore the degree to which health risks affect older adults, and advocate for active engagement in practices for managing health risks. Methods Using purposive sampling, three districts of Beijing (Xicheng District, Fengtai District, and Daxing District, respectively) were selected for the research. Qualitative semi-structured and in-depth interviews were conducted with 70 community-dwelling older adults who participated in the study. Data were extracted and analyzed based on a thematic framework approach. Results Three main themes were identified: (i) the anxieties of older adults concerning health risks in ageing; (ii) the priorities of older adults for health risk management in ageing; (iii) the expectations of older adults for health risk management in ageing. The primary health concerns among older adults included disease incidence and function decline. It was found that basic health management emerged as a critical need for older adults to mitigate health risks. Moreover, it was observed that healthcare support for older adults from familial, institutional, and governmental levels exhibited varying degrees of inadequacy. Conclusions The primary source of anxieties among older adults regarding health risks predominantly stems from a perceived sense of health deprivation. It is often compounded by persistent barriers to primary care of priorities in managing health risks among older adults. In addition, the expectations of older adults for health risk management emphasize the necessity for integrated care approaches. Therefore, further research should give priority to the prevention and management of health risks, aim to reduce anxieties, provide integrated care to meet the primary needs and expectations of older adults, and ultimately strive toward the overarching goal of promoting health and longevity.
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